CO6160247A2 - Variantes de pululanasa con productividad elevada y metodos para prepararlas - Google Patents
Variantes de pululanasa con productividad elevada y metodos para prepararlasInfo
- Publication number
- CO6160247A2 CO6160247A2 CO09017236A CO09017236A CO6160247A2 CO 6160247 A2 CO6160247 A2 CO 6160247A2 CO 09017236 A CO09017236 A CO 09017236A CO 09017236 A CO09017236 A CO 09017236A CO 6160247 A2 CO6160247 A2 CO 6160247A2
- Authority
- CO
- Colombia
- Prior art keywords
- group
- expression construct
- seq
- composition
- host organism
- Prior art date
Links
- 239000002773 nucleotide Substances 0.000 abstract 3
- 125000003729 nucleotide group Chemical group 0.000 abstract 3
- 108020004414 DNA Proteins 0.000 abstract 2
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 abstract 1
- 241000228245 Aspergillus niger Species 0.000 abstract 1
- 241000194108 Bacillus licheniformis Species 0.000 abstract 1
- 244000063299 Bacillus subtilis Species 0.000 abstract 1
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 abstract 1
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 241000233866 Fungi Species 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 241000235070 Saccharomyces Species 0.000 abstract 1
- 241000499912 Trichoderma reesei Species 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 210000003527 eukaryotic cell Anatomy 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
- C12N9/2457—Pullulanase (3.2.1.41)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01041—Pullulanase (3.2.1.41)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US83973506P | 2006-08-23 | 2006-08-23 | |
| US90324707P | 2007-02-23 | 2007-02-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CO6160247A2 true CO6160247A2 (es) | 2010-05-20 |
Family
ID=38969548
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CO09017236A CO6160247A2 (es) | 2006-08-23 | 2009-02-20 | Variantes de pululanasa con productividad elevada y metodos para prepararlas |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US7968691B2 (https=) |
| EP (1) | EP2054512B1 (https=) |
| JP (1) | JP2010501181A (https=) |
| KR (1) | KR20090041410A (https=) |
| CN (2) | CN101506360B (https=) |
| BR (1) | BRPI0715619A2 (https=) |
| CA (2) | CA2663948C (https=) |
| CO (1) | CO6160247A2 (https=) |
| EA (1) | EA017712B1 (https=) |
| ES (1) | ES2547857T3 (https=) |
| HU (1) | HUE025068T2 (https=) |
| MX (1) | MX2009001717A (https=) |
| PL (1) | PL2054512T3 (https=) |
| WO (1) | WO2008024372A2 (https=) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7968691B2 (en) * | 2006-08-23 | 2011-06-28 | Danisco Us Inc. | Pullulanase variants with increased productivity |
| IN2012DN00427A (https=) | 2009-07-01 | 2015-05-15 | Bayer Bioscience Nv | |
| ES2676895T5 (es) | 2013-03-11 | 2022-04-27 | Danisco Us Inc | Variantes combinatorias de alfa-amilasa |
| EP3022300B1 (en) * | 2013-07-17 | 2018-07-11 | Novozymes A/S | Pullulanase chimeras and polynucleotides encoding same |
| BR112016009416A2 (pt) | 2013-10-28 | 2017-10-03 | Danisco Us Inc | Levedura seca ativa geneticamente modificada de grande escala |
| MX2016006489A (es) | 2013-11-20 | 2016-08-03 | Danisco Us Inc | Alfa-amilasas variantes que tienen susceptibilidad reducida a la escision por proteasas y metodos de uso. |
| WO2016062875A2 (en) | 2014-10-23 | 2016-04-28 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
| CN108064306B (zh) | 2014-12-23 | 2022-11-01 | 营养与生物科学美国4公司 | 酶促产生的纤维素 |
| EP3253869A4 (en) * | 2015-02-04 | 2018-11-07 | Nanjing Bestzyme Bio-Engineering Co. Ltd. | Truncated pullulanases, methods of production, and methods of use thereof |
| US20180030456A1 (en) | 2015-02-19 | 2018-02-01 | Danisco Us Inc. | Enhanced protein expression |
| CN109071615A (zh) | 2016-03-04 | 2018-12-21 | 丹尼斯科美国公司 | 用于在微生物中产生蛋白质的工程化核糖体启动子 |
| CN105925594A (zh) * | 2016-06-13 | 2016-09-07 | 广西大学 | 生淀粉糖化酶及其制备方法与其在生淀粉水解和生料同步糖化发酵产酒精中的应用 |
| US20190367932A1 (en) | 2017-01-23 | 2019-12-05 | Danisco Us Inc. | Methods and compositions for obtaining natural comptence in bacillus host cells |
| JP7231228B2 (ja) | 2017-02-24 | 2023-03-01 | ダニスコ・ユーエス・インク | バチルス・リケニフォルミスにおける増加したタンパク質産生のための組成物及び方法 |
| EP3703661A1 (en) | 2017-11-02 | 2020-09-09 | Danisco US Inc. | Freezing point depressed solid matrix compositions for melt granulation of enzymes |
| KR102715197B1 (ko) | 2018-01-03 | 2024-10-08 | 다니스코 유에스 인크. | 증가된 단백질 생산을 위한 돌연변이체 및 유전자 변형된 바실러스 세포 및 이의 방법 |
| WO2020172465A1 (en) * | 2019-02-20 | 2020-08-27 | Duke University | Compositions and methods for the treatment of genetic diseases |
| CN110184258B (zh) * | 2019-06-11 | 2020-11-06 | 南京林业大学 | 一种普鲁兰酶突变体 |
| CN110791513B (zh) * | 2019-11-15 | 2021-05-25 | 中国科学院南海海洋研究所 | 一种密码子优化的玉足海参α-淀粉酶基因及其重组表达载体与应用 |
| CN111662933B (zh) * | 2020-06-24 | 2023-03-24 | 江南大学 | 一种利用重组酵母进行高支链淀粉酒精发酵的方法 |
| US12508305B2 (en) * | 2020-12-26 | 2025-12-30 | Duke University | Compositions and methods for treating and/or preventing glycogen storage disease type III |
| EP4387982A2 (en) * | 2021-08-20 | 2024-06-26 | Danisco US Inc. | Methods and compositions for enhanced protein production in bacillus cells |
| EP4525615A2 (en) | 2022-05-14 | 2025-03-26 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4760025A (en) * | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
| US5264366A (en) * | 1984-05-29 | 1993-11-23 | Genencor, Inc. | Protease deficient bacillus |
| US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| DE3650202T3 (de) | 1985-08-29 | 2004-06-03 | Genencor International, Inc., South San Francisco | Expression von heterologen Polypeptiden in filamentösen Pilzen, Verfahren zu deren Herstellung und Vektoren zu deren Herstellung. |
| DK0605040T3 (da) * | 1992-12-28 | 2000-03-13 | Genencor Int | Pullulanase, mikroorganismer producerende denne fremgangsmåde il fremstilling af denne pullulanase og anvendelse deraf |
| US5605793A (en) * | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US6537776B1 (en) * | 1999-06-14 | 2003-03-25 | Diversa Corporation | Synthetic ligation reassembly in directed evolution |
| JP4307563B2 (ja) * | 1997-04-07 | 2009-08-05 | ユニリーバー・ナームローゼ・ベンノートシャープ | 糸状菌、特にアスペルギルス属に属する糸状菌のアグロバクテリウム媒介性形質転換 |
| EP0981628A1 (en) * | 1997-05-06 | 2000-03-01 | E.I. Dupont De Nemours And Company | Corn pullulanase |
| JP2002505108A (ja) * | 1998-03-04 | 2002-02-19 | ジェネンコア インターナショナル インコーポレーテッド | プルラナーゼの修飾形態 |
| CN1415016B (zh) * | 1999-12-30 | 2012-07-11 | 金克克国际有限公司 | Trichoderma reesei木聚糖酶 |
| US6350599B1 (en) * | 2000-01-12 | 2002-02-26 | Novozymes A/S | Pullulanase variants and methods for preparing such variants with predetermined properties |
| AU2663801A (en) * | 2000-01-12 | 2001-07-24 | Novozymes A/S | Pullulanase variants and methods for preparing such variants with predetermined properties |
| US7886330B2 (en) | 2000-06-30 | 2011-02-08 | Jlb Ventures Llc | Enhanced home entertainment system with removable long-term storage |
| WO2002014490A2 (en) | 2000-08-11 | 2002-02-21 | Genencor International, Inc. | Bacillus transformation, transformants and mutant libraries |
| US7713725B2 (en) * | 2002-09-10 | 2010-05-11 | Danisco Us Inc. | Induction of gene expression using a high concentration sugar mixture |
| US7968691B2 (en) * | 2006-08-23 | 2011-06-28 | Danisco Us Inc. | Pullulanase variants with increased productivity |
-
2007
- 2007-08-20 US US11/841,526 patent/US7968691B2/en not_active Expired - Fee Related
- 2007-08-21 CN CN200780031317.XA patent/CN101506360B/zh not_active Expired - Fee Related
- 2007-08-21 BR BRPI0715619-7A patent/BRPI0715619A2/pt not_active Application Discontinuation
- 2007-08-21 HU HUE07837179A patent/HUE025068T2/en unknown
- 2007-08-21 MX MX2009001717A patent/MX2009001717A/es active IP Right Grant
- 2007-08-21 CN CN201410181518.XA patent/CN103898080B/zh not_active Expired - Fee Related
- 2007-08-21 EP EP20070837179 patent/EP2054512B1/en not_active Not-in-force
- 2007-08-21 CA CA2663948A patent/CA2663948C/en not_active Expired - Fee Related
- 2007-08-21 WO PCT/US2007/018523 patent/WO2008024372A2/en not_active Ceased
- 2007-08-21 CA CA2949536A patent/CA2949536A1/en not_active Abandoned
- 2007-08-21 PL PL07837179T patent/PL2054512T3/pl unknown
- 2007-08-21 JP JP2009525612A patent/JP2010501181A/ja active Pending
- 2007-08-21 ES ES07837179.6T patent/ES2547857T3/es active Active
- 2007-08-21 KR KR1020097003544A patent/KR20090041410A/ko not_active Abandoned
- 2007-08-21 EA EA200970212A patent/EA017712B1/ru not_active IP Right Cessation
-
2009
- 2009-02-20 CO CO09017236A patent/CO6160247A2/es unknown
-
2011
- 2011-02-28 US US13/036,749 patent/US8354101B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| KR20090041410A (ko) | 2009-04-28 |
| EA017712B1 (ru) | 2013-02-28 |
| US7968691B2 (en) | 2011-06-28 |
| JP2010501181A (ja) | 2010-01-21 |
| WO2008024372A2 (en) | 2008-02-28 |
| EP2054512A2 (en) | 2009-05-06 |
| HK1135137A1 (en) | 2010-05-28 |
| BRPI0715619A2 (pt) | 2013-06-25 |
| CN103898080B (zh) | 2017-04-12 |
| US20080108126A1 (en) | 2008-05-08 |
| CA2663948C (en) | 2017-01-03 |
| US8354101B2 (en) | 2013-01-15 |
| CA2949536A1 (en) | 2008-02-28 |
| CN101506360A (zh) | 2009-08-12 |
| ES2547857T3 (es) | 2015-10-09 |
| EP2054512B1 (en) | 2015-04-22 |
| CA2663948A1 (en) | 2008-02-28 |
| HUE025068T2 (en) | 2016-01-28 |
| MX2009001717A (es) | 2009-02-25 |
| US20110281326A1 (en) | 2011-11-17 |
| PL2054512T3 (pl) | 2015-09-30 |
| CN101506360B (zh) | 2014-06-11 |
| EA200970212A1 (ru) | 2009-08-28 |
| WO2008024372A3 (en) | 2008-04-24 |
| CN103898080A (zh) | 2014-07-02 |
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