CO6160247A2 - Variantes de pululanasa con productividad elevada y metodos para prepararlas - Google Patents
Variantes de pululanasa con productividad elevada y metodos para prepararlasInfo
- Publication number
- CO6160247A2 CO6160247A2 CO09017236A CO09017236A CO6160247A2 CO 6160247 A2 CO6160247 A2 CO 6160247A2 CO 09017236 A CO09017236 A CO 09017236A CO 09017236 A CO09017236 A CO 09017236A CO 6160247 A2 CO6160247 A2 CO 6160247A2
- Authority
- CO
- Colombia
- Prior art keywords
- group
- expression construct
- seq
- composition
- host organism
- Prior art date
Links
- 239000002773 nucleotide Substances 0.000 abstract 3
- 125000003729 nucleotide group Chemical group 0.000 abstract 3
- 108020004414 DNA Proteins 0.000 abstract 2
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 abstract 1
- 241000228245 Aspergillus niger Species 0.000 abstract 1
- 241000194108 Bacillus licheniformis Species 0.000 abstract 1
- 244000063299 Bacillus subtilis Species 0.000 abstract 1
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 abstract 1
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 241000233866 Fungi Species 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 241000235070 Saccharomyces Species 0.000 abstract 1
- 241000499912 Trichoderma reesei Species 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 210000003527 eukaryotic cell Anatomy 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
- C12N9/2457—Pullulanase (3.2.1.41)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01041—Pullulanase (3.2.1.41)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US83973506P | 2006-08-23 | 2006-08-23 | |
| US90324707P | 2007-02-23 | 2007-02-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CO6160247A2 true CO6160247A2 (es) | 2010-05-20 |
Family
ID=38969548
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CO09017236A CO6160247A2 (es) | 2006-08-23 | 2009-02-20 | Variantes de pululanasa con productividad elevada y metodos para prepararlas |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US7968691B2 (https=) |
| EP (1) | EP2054512B1 (https=) |
| JP (1) | JP2010501181A (https=) |
| KR (1) | KR20090041410A (https=) |
| CN (2) | CN103898080B (https=) |
| BR (1) | BRPI0715619A2 (https=) |
| CA (2) | CA2663948C (https=) |
| CO (1) | CO6160247A2 (https=) |
| EA (1) | EA017712B1 (https=) |
| ES (1) | ES2547857T3 (https=) |
| HU (1) | HUE025068T2 (https=) |
| MX (1) | MX2009001717A (https=) |
| PL (1) | PL2054512T3 (https=) |
| WO (1) | WO2008024372A2 (https=) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7968691B2 (en) * | 2006-08-23 | 2011-06-28 | Danisco Us Inc. | Pullulanase variants with increased productivity |
| US20120096600A1 (en) | 2009-07-01 | 2012-04-19 | Rene Ruiter | Methods and means for obtaining plants with enhanced glyphosate tolerance |
| DK2970931T3 (en) | 2013-03-11 | 2018-01-22 | Danisco Us Inc | COMBINATORY ALPHA AMYLASE VARIATIONS |
| HUE040545T2 (hu) | 2013-07-17 | 2019-03-28 | Novozymes As | Pullulanáz kimérák és az azokat kódoló polinukleotidok |
| BR112016009416A2 (pt) | 2013-10-28 | 2017-10-03 | Danisco Us Inc | Levedura seca ativa geneticamente modificada de grande escala |
| US20160272957A1 (en) | 2013-11-20 | 2016-09-22 | Danisco Us Inc. | Variant alpha-amylases having reduced susceptibility to protease cleavage, and methods of use, thereof |
| WO2016062875A2 (en) | 2014-10-23 | 2016-04-28 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
| CN107109450A (zh) | 2014-12-23 | 2017-08-29 | 纳幕尔杜邦公司 | 酶促产生的纤维素 |
| JP6505240B2 (ja) * | 2015-02-04 | 2019-04-24 | ナンジン ベストザイム バイオ−エンジニアリング コンパニー リミテッド | 切断型プルラナーゼの製造および使用方法 |
| EP3259358B1 (en) * | 2015-02-19 | 2024-06-19 | Danisco US Inc. | Enhanced protein expression |
| WO2017152169A1 (en) | 2016-03-04 | 2017-09-08 | Danisco Us Inc. | Engineered ribosomal promoters for protein production in microorganisms |
| CN105925594A (zh) * | 2016-06-13 | 2016-09-07 | 广西大学 | 生淀粉糖化酶及其制备方法与其在生淀粉水解和生料同步糖化发酵产酒精中的应用 |
| EP3571217B1 (en) | 2017-01-23 | 2023-06-21 | Danisco US Inc. | Methods and compositions for obtaining natural competence in bacillus host cells |
| EP3585910B1 (en) | 2017-02-24 | 2024-03-20 | Danisco US Inc. | Compositions and methods for increased protein production in bacillus licheniformis |
| EP3703661A1 (en) | 2017-11-02 | 2020-09-09 | Danisco US Inc. | Freezing point depressed solid matrix compositions for melt granulation of enzymes |
| JP7280269B2 (ja) | 2018-01-03 | 2023-05-23 | ダニスコ・ユーエス・インク | タンパク質の産生増加のための変異及び遺伝子改変バチルス属（ｂａｃｉｌｌｕｓ）細胞、並びにその方法 |
| WO2020172465A1 (en) * | 2019-02-20 | 2020-08-27 | Duke University | Compositions and methods for the treatment of genetic diseases |
| CN110184258B (zh) * | 2019-06-11 | 2020-11-06 | 南京林业大学 | 一种普鲁兰酶突变体 |
| CN110791513B (zh) * | 2019-11-15 | 2021-05-25 | 中国科学院南海海洋研究所 | 一种密码子优化的玉足海参α-淀粉酶基因及其重组表达载体与应用 |
| CN111662933B (zh) * | 2020-06-24 | 2023-03-24 | 江南大学 | 一种利用重组酵母进行高支链淀粉酒精发酵的方法 |
| US12508305B2 (en) * | 2020-12-26 | 2025-12-30 | Duke University | Compositions and methods for treating and/or preventing glycogen storage disease type III |
| WO2023023642A2 (en) * | 2021-08-20 | 2023-02-23 | Danisco Us Inc. | Methods and compositions for enhanced protein production in bacillus cells |
| CN119630280A (zh) | 2022-05-14 | 2025-03-14 | 诺维信公司 | 用于预防、处理、抑制和/或消除植物病原性侵染和感染的组合物和方法 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4760025A (en) | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
| US5264366A (en) | 1984-05-29 | 1993-11-23 | Genencor, Inc. | Protease deficient bacillus |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| DE3650202T3 (de) | 1985-08-29 | 2004-06-03 | Genencor International, Inc., South San Francisco | Expression von heterologen Polypeptiden in filamentösen Pilzen, Verfahren zu deren Herstellung und Vektoren zu deren Herstellung. |
| EP0605040B1 (fr) * | 1992-12-28 | 1999-08-11 | Genencor International, Inc. | Pullulanase, microorganismes la produisant, procédés de préparation de cette pullulanase et utilisations de celle-ci |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US6537776B1 (en) | 1999-06-14 | 2003-03-25 | Diversa Corporation | Synthetic ligation reassembly in directed evolution |
| US6255115B1 (en) | 1997-04-07 | 2001-07-03 | Unilever Patent Holdings Bv | Agrobacterium mediated transformation of moulds, in particular those belonging to the genus Aspergillus |
| CA2284688C (en) | 1997-05-06 | 2010-02-16 | E.I. Du Pont De Nemours And Company | Corn pullulanase |
| BR9908422A (pt) * | 1998-03-04 | 2000-10-31 | Genencor Int | Pululanase modificada, ácido nucleico, vetor de expressão, microorganismo hospedeiro, processo para a produção de uma pululanase modificada em uma célula hospedeira, composição enzimática, processo para sacarificação de amido, e, b. licheniformis |
| ATE288493T1 (de) | 1999-12-30 | 2005-02-15 | Genencor Int | Trichoderma reesei xylanase |
| US6350599B1 (en) | 2000-01-12 | 2002-02-26 | Novozymes A/S | Pullulanase variants and methods for preparing such variants with predetermined properties |
| EP1250423B1 (en) | 2000-01-12 | 2008-09-03 | Novozymes A/S | Pullulanase variants and methods for preparing such variants with predetermined properties |
| US7886330B2 (en) | 2000-06-30 | 2011-02-08 | Jlb Ventures Llc | Enhanced home entertainment system with removable long-term storage |
| CA2418317A1 (en) | 2000-08-11 | 2002-02-21 | Genencor International, Inc. | Bacillus transformation, transformants and mutant libraries |
| CA2498213C (en) | 2002-09-10 | 2013-07-16 | Genencor International, Inc. | Induction of gene expression using a high concentration sugar mixture |
| US7968691B2 (en) * | 2006-08-23 | 2011-06-28 | Danisco Us Inc. | Pullulanase variants with increased productivity |
-
2007
- 2007-08-20 US US11/841,526 patent/US7968691B2/en not_active Expired - Fee Related
- 2007-08-21 MX MX2009001717A patent/MX2009001717A/es active IP Right Grant
- 2007-08-21 PL PL07837179T patent/PL2054512T3/pl unknown
- 2007-08-21 CA CA2663948A patent/CA2663948C/en not_active Expired - Fee Related
- 2007-08-21 CN CN201410181518.XA patent/CN103898080B/zh not_active Expired - Fee Related
- 2007-08-21 BR BRPI0715619-7A patent/BRPI0715619A2/pt not_active Application Discontinuation
- 2007-08-21 ES ES07837179.6T patent/ES2547857T3/es active Active
- 2007-08-21 HU HUE07837179A patent/HUE025068T2/en unknown
- 2007-08-21 JP JP2009525612A patent/JP2010501181A/ja active Pending
- 2007-08-21 WO PCT/US2007/018523 patent/WO2008024372A2/en not_active Ceased
- 2007-08-21 EP EP20070837179 patent/EP2054512B1/en not_active Not-in-force
- 2007-08-21 KR KR1020097003544A patent/KR20090041410A/ko not_active Abandoned
- 2007-08-21 CA CA2949536A patent/CA2949536A1/en not_active Abandoned
- 2007-08-21 CN CN200780031317.XA patent/CN101506360B/zh not_active Expired - Fee Related
- 2007-08-21 EA EA200970212A patent/EA017712B1/ru not_active IP Right Cessation
-
2009
- 2009-02-20 CO CO09017236A patent/CO6160247A2/es unknown
-
2011
- 2011-02-28 US US13/036,749 patent/US8354101B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP2010501181A (ja) | 2010-01-21 |
| CN101506360B (zh) | 2014-06-11 |
| US8354101B2 (en) | 2013-01-15 |
| MX2009001717A (es) | 2009-02-25 |
| HK1135137A1 (en) | 2010-05-28 |
| US20110281326A1 (en) | 2011-11-17 |
| PL2054512T3 (pl) | 2015-09-30 |
| EA200970212A1 (ru) | 2009-08-28 |
| EA017712B1 (ru) | 2013-02-28 |
| CA2663948A1 (en) | 2008-02-28 |
| CA2949536A1 (en) | 2008-02-28 |
| EP2054512B1 (en) | 2015-04-22 |
| ES2547857T3 (es) | 2015-10-09 |
| CN103898080B (zh) | 2017-04-12 |
| BRPI0715619A2 (pt) | 2013-06-25 |
| WO2008024372A3 (en) | 2008-04-24 |
| EP2054512A2 (en) | 2009-05-06 |
| HUE025068T2 (en) | 2016-01-28 |
| CA2663948C (en) | 2017-01-03 |
| CN103898080A (zh) | 2014-07-02 |
| US7968691B2 (en) | 2011-06-28 |
| US20080108126A1 (en) | 2008-05-08 |
| KR20090041410A (ko) | 2009-04-28 |
| CN101506360A (zh) | 2009-08-12 |
| WO2008024372A2 (en) | 2008-02-28 |
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