JP2009095342A - ハタケシメジの菌床栽培方法 - Google Patents
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Abstract
【解決手段】ハタケシメジの菌床栽培方法における子実体原基の生育工程において、相対湿度を段階的に下げることを特徴とするハタケシメジの菌床栽培方法を提供する。例えば、子実体原基の生育工程において相対湿度を90〜120%の範囲内で段階的に下げることが例示される。また更に、前期生育工程として相対湿度110〜120%の範囲内の加湿条件下での生育、後期生育工程として相対湿度90〜105%の範囲内の加湿条件下での生育を行うことが例示される。本発明により、高品質で製品化率が高く、かつ保存性が良好な株状ハタケシメジ子実体を安定的に生産することが可能となる。
【選択図】なし
Description
本発明の菌床栽培方法に好適なハタケシメジの菌株の例としては、ハタケシメジK−3303株(FERM BP−4347)、ハタケシメジK−3304株(FERM BP−4348)、ハタケシメジK−3305株(FERM BP−4349)、ハタケシメジF−623株(FERM P−13165)、ハタケシメジF−1154株(FERM P−13166)、ハタケシメジF−1488株(FERM P−13167)、及びこれらの変異株等があるが、本発明で使用できる菌株はこれらの菌株に限られるものではない。
PGY液体培地(組成:グルコース2.0%、ペプトン0.2%、酵母エキス0.2%、KH2PO40.05%、及びMgSO4・7H2O0.05%、pH6.0)100mLにハタケシメジK−3304(FERM BP−4348)を接種して、三角フラスコで25℃で14日間培養し液体種菌とした。一方、ポリプロピレン製の広口培養ビン(1100mL)に、1ビン当たりの培地組成が鋸屑(スギ材)134g、米糠130g、メタケイ酸アルミン酸マグネシウム〔富士化学工業(株)製、商品名ノイシリンFH1〕2.6g、炭酸カルシウム〔ナカライテスク(株)製、試薬一級〕6.5g、クエン酸一水塩〔ナカライテスク(株)製、試薬一級〕3.9g、水分含量63%となるように各種基材を良く混合し湿潤状態にしたものを圧詰して、中央に直径1cm程度の穴を開け、打栓後118℃、90分間高圧蒸気殺菌を行い、放冷して固形培養基としたものを準備した。これに上記の液体種菌約30mLを接種し、温度25℃、湿度55%の条件の下、培養基に見掛け上菌糸が廻るまで約60日間培養し、固体種菌を作成した。
Claims (12)
- ハタケシメジの菌床栽培方法における子実体原基の生育工程において、相対湿度を段階的に下げることを特徴とするハタケシメジの菌床栽培方法。
- 子実体原基の生育工程において、相対湿度を90〜120%の範囲内で段階的に下げる請求項1記載のハタケシメジの菌床栽培方法。
- 子実体原基の生育工程において、前期生育工程として相対湿度110〜120%の範囲内の加湿条件下での生育、及び後期生育工程として相対湿度90〜105%の範囲内の加湿条件下での生育を行う請求項2記載のハタケシメジの菌床栽培方法。
- 子実体原基の前期生育工程を相対湿度110〜115%の範囲内又は115〜120%の範囲内の加湿条件下での生育、後期生育工程を相対湿度90〜100%の範囲内又は95〜105%の範囲内の加湿条件下での生育を行う請求項3記載のハタケシメジの菌床栽培方法。
- 子実体原基の後期生育工程において、相対湿度95〜105%の範囲内の加湿条件下で生育を行った後、さらに相対湿度90〜100%の範囲内の加湿条件下で相対湿度を段階的に下げて生育を行なう請求項3記載のハタケシメジの菌床栽培方法。
- 14〜17℃で3〜10日間の前期生育工程及び14〜17℃で3〜10日間の後期生育工程で行う請求項3〜5記載のいずれか1項に記載のハタケシメジの菌床栽培方法。
- 子実体原基の生育工程をCO2濃度1000〜2000ppmの環境条件下で行う請求項1〜6のいずれか1項に記載のハタケシメジの菌床栽培方法。
- 芽出し工程での子実体原基形成を相対湿度115〜120%の範囲内の加湿条件下、子実体原基の前期生育工程を相対湿度110〜120%の範囲内の加湿条件下での生育、及び後期生育工程を相対湿度90〜105%の範囲内の加湿条件下での生育を行う請求項2記載のハタケシメジの菌床栽培方法。
- 子実体原基の前期生育工程を相対湿度110〜115%の範囲内又は115〜120%の範囲内の加湿条件下での生育、後期生育工程を相対湿度90〜100%の範囲内又は95〜105%の範囲内の加湿条件下での生育を行う請求項8記載のハタケシメジの菌床栽培方法。
- 子実体原基の後期生育工程において、相対湿度95〜105%の範囲内の加湿条件下で生育を行った後、さらに相対湿度90〜100%の範囲内の加湿条件下で相対湿度を段階的に下げて生育を行なう請求項8記載のハタケシメジの菌床栽培方法。
- 15〜17℃で9〜12日間の芽出し工程、14〜17℃で3〜10日間の前期生育工程、及び14〜17℃で3〜10日間の後期生育工程で行う請求項8〜10のいずれか1項に記載のハタケシメジの菌床栽培方法。
- 芽出し工程をCO2濃度1500ppm以下の環境条件下、前期生育工程及び後期生育工程をCO2濃度1000〜2000ppmの環境条件下で行う請求項8〜11のいずれか1項に記載のハタケシメジの菌床栽培方法。
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN104322281A (zh) * | 2014-10-22 | 2015-02-04 | 江苏菇本堂生物科技股份有限公司 | 一种瓶栽鹿茸菇出菇期培育方法 |
CN111887100A (zh) * | 2020-07-22 | 2020-11-06 | 贵州省贵福菌业发展有限公司 | 食用菌废菌棒活化再利用制备鹿茸菇栽培基质及制备方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0745A (ja) * | 1993-06-14 | 1995-01-06 | New Oji Paper Co Ltd | ハタケシメジの室内栽培法 |
JPH10178890A (ja) * | 1996-12-26 | 1998-07-07 | Takara Aguri Kk | ハタケシメジの人工栽培方法 |
JP2005137213A (ja) * | 2003-11-04 | 2005-06-02 | Sanki Eng Co Ltd | きのこ栽培方法とその方法に用いる人工栽培室 |
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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JPH0745A (ja) * | 1993-06-14 | 1995-01-06 | New Oji Paper Co Ltd | ハタケシメジの室内栽培法 |
JPH10178890A (ja) * | 1996-12-26 | 1998-07-07 | Takara Aguri Kk | ハタケシメジの人工栽培方法 |
JP2005137213A (ja) * | 2003-11-04 | 2005-06-02 | Sanki Eng Co Ltd | きのこ栽培方法とその方法に用いる人工栽培室 |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104322281A (zh) * | 2014-10-22 | 2015-02-04 | 江苏菇本堂生物科技股份有限公司 | 一种瓶栽鹿茸菇出菇期培育方法 |
CN111887100A (zh) * | 2020-07-22 | 2020-11-06 | 贵州省贵福菌业发展有限公司 | 食用菌废菌棒活化再利用制备鹿茸菇栽培基质及制备方法 |
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