JP2008510455A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2008510455A5 JP2008510455A5 JP2007523157A JP2007523157A JP2008510455A5 JP 2008510455 A5 JP2008510455 A5 JP 2008510455A5 JP 2007523157 A JP2007523157 A JP 2007523157A JP 2007523157 A JP2007523157 A JP 2007523157A JP 2008510455 A5 JP2008510455 A5 JP 2008510455A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- present
- light output
- sample
- template nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000007523 nucleic acids Chemical class 0.000 claims description 52
- 108020004707 nucleic acids Proteins 0.000 claims description 50
- 102000039446 nucleic acids Human genes 0.000 claims description 50
- 238000000034 method Methods 0.000 claims description 44
- 230000003321 amplification Effects 0.000 claims description 21
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 21
- 238000006243 chemical reaction Methods 0.000 claims description 16
- 102000004190 Enzymes Human genes 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 9
- 239000000758 substrate Substances 0.000 claims description 7
- 238000005415 bioluminescence Methods 0.000 claims description 6
- 230000029918 bioluminescence Effects 0.000 claims description 6
- 238000003556 assay Methods 0.000 claims description 5
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 claims description 4
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 claims description 4
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 claims description 4
- 108060001084 Luciferase Proteins 0.000 claims description 4
- 239000005089 Luciferase Substances 0.000 claims description 4
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 claims description 4
- 102000004523 Sulfate Adenylyltransferase Human genes 0.000 claims description 3
- 108010022348 Sulfate adenylyltransferase Proteins 0.000 claims description 3
- 230000002068 genetic effect Effects 0.000 claims description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 2
- 238000012544 monitoring process Methods 0.000 claims description 2
- 230000007423 decrease Effects 0.000 claims 5
- 239000002253 acid Substances 0.000 claims 2
- 239000000872 buffer Substances 0.000 claims 2
- MWMOPIVLTLEUJO-UHFFFAOYSA-N 2-oxopropanoic acid;phosphoric acid Chemical compound OP(O)(O)=O.CC(=O)C(O)=O MWMOPIVLTLEUJO-UHFFFAOYSA-N 0.000 claims 1
- 241000606161 Chlamydia Species 0.000 claims 1
- 101100409013 Mesembryanthemum crystallinum PPD gene Proteins 0.000 claims 1
- 235000010730 Ulex europaeus Nutrition 0.000 claims 1
- 240000003864 Ulex europaeus Species 0.000 claims 1
- 230000001351 cycling effect Effects 0.000 claims 1
- 238000002405 diagnostic procedure Methods 0.000 claims 1
- 201000001371 inclusion conjunctivitis Diseases 0.000 claims 1
- 239000004615 ingredient Substances 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 244000052769 pathogen Species 0.000 claims 1
- 230000001717 pathogenic effect Effects 0.000 claims 1
- 101150063097 ppdK gene Proteins 0.000 claims 1
- 206010044325 trachoma Diseases 0.000 claims 1
- 108020004414 DNA Proteins 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 239000012131 assay buffer Substances 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 1
- 235000011180 diphosphates Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0416944.7A GB0416944D0 (en) | 2004-07-29 | 2004-07-29 | Method for determining the amount of template nucleic acid present in a sample |
| GB0416944.7 | 2004-07-29 | ||
| PCT/GB2005/002998 WO2006010948A1 (en) | 2004-07-29 | 2005-07-29 | Method for determining the amount of template nucleic acid present in a sample |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2008510455A JP2008510455A (ja) | 2008-04-10 |
| JP2008510455A5 true JP2008510455A5 (https=) | 2008-09-11 |
| JP5055118B2 JP5055118B2 (ja) | 2012-10-24 |
Family
ID=32947663
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007523157A Expired - Fee Related JP5055118B2 (ja) | 2004-07-29 | 2005-07-29 | 試料中に存在するテンプレート核酸の量を決定するための方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US8309308B2 (https=) |
| EP (1) | EP1776467B8 (https=) |
| JP (1) | JP5055118B2 (https=) |
| CN (1) | CN101035908B (https=) |
| AU (1) | AU2005266143B2 (https=) |
| CA (1) | CA2575217C (https=) |
| ES (1) | ES2557158T3 (https=) |
| GB (1) | GB0416944D0 (https=) |
| WO (1) | WO2006010948A1 (https=) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0517005D0 (en) * | 2005-08-19 | 2005-09-28 | Enigma Diagnostics Ltd | Analytical method and kit |
| JP5520449B2 (ja) * | 2007-03-06 | 2014-06-11 | 株式会社ビー・エム・エル | 核酸の検出方法 |
| JP5570731B2 (ja) * | 2008-02-28 | 2014-08-13 | 旭化成ファーマ株式会社 | ピロリン酸の測定方法 |
| GB0812862D0 (en) | 2008-07-14 | 2008-08-20 | Lumora Ltd | Nucleic acid amplification |
| US9677981B2 (en) | 2013-03-15 | 2017-06-13 | 3M Innovative Properties Company | Sample concentrator and method of use |
| US10364458B2 (en) | 2014-07-16 | 2019-07-30 | Tangen Biosciences, Inc. | Isothermal methods for amplifying nucleic acid samples |
| EP3215260B1 (en) | 2014-11-03 | 2020-01-15 | Tangen Biosciences Inc. | Apparatus and method for cell, spore, or virus capture and disruption |
| KR20210020864A (ko) * | 2018-03-13 | 2021-02-24 | 사르말, 인크. | 단일 분자 서열분석 방법 |
| US12325883B2 (en) | 2019-01-15 | 2025-06-10 | Neogen Food Safety Us Holdco Corporation | Loop-mediated isothermal amplification primers for Shiga toxin-producing E. coli (STEC) detection |
| US11008627B2 (en) | 2019-08-15 | 2021-05-18 | Talis Biomedical Corporation | Diagnostic system |
| WO2021192965A1 (ja) * | 2020-03-26 | 2021-09-30 | Ｐｈｃホールディングス株式会社 | 等温核酸増幅方法および等温核酸増幅装置 |
| CN115216521B (zh) * | 2022-08-05 | 2025-03-21 | 苏州卫生职业技术学院 | 核酸扩增产物检测试剂及检测方法 |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5498523A (en) | 1988-07-12 | 1996-03-12 | President And Fellows Of Harvard College | DNA sequencing with pyrophosphatase |
| FR2674254B1 (fr) | 1991-03-20 | 1995-10-06 | Univ Reims Champagne Ardenne | Detection non radioactive de la presence d'un acide nucleique determine dans un echantillon biologique. |
| US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| GB9210176D0 (en) | 1992-05-12 | 1992-06-24 | Cemu Bioteknik Ab | Chemical method |
| WO1995025180A1 (en) * | 1994-03-16 | 1995-09-21 | Gen-Probe Incorporated | Isothermal strand displacement nucleic acid amplification |
| DE69633171T2 (de) | 1995-01-20 | 2005-08-18 | The Secretary Of State For Defence, Salisbury | Mutierte luziferasen |
| JP2001501454A (ja) | 1996-05-17 | 2001-02-06 | イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー | プライマーに制御された増幅産物の蛍光検出による標的細菌の同定 |
| JP4540754B2 (ja) | 1996-06-04 | 2010-09-08 | ユニバーシティ オブ ユタ リサーチ ファウンデーション | Ｐｃｒ中のハイブリダイゼーションのモニタリング |
| GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
| JPH10108679A (ja) | 1996-10-03 | 1998-04-28 | Kikkoman Corp | ピルベートオルトフォスフェートジキナーゼ遺伝子、組み換え体ｄｎａ及びピルベートオルトフォスフェートジキナーゼの製造法 |
| US20030104372A1 (en) | 1996-12-23 | 2003-06-05 | Pyrosequencing Ab. | Allele specific primer extension |
| GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
| AU7813698A (en) * | 1997-06-06 | 1998-12-21 | Nexstar Pharmaceuticals, Inc. | Nucleic acid detection |
| CA2301828A1 (en) | 1997-09-19 | 1999-03-25 | Promega Corporation | Thermostable luciferases and methods of production |
| GB9803382D0 (en) | 1998-02-19 | 1998-04-15 | Secr Defence | Detection system |
| GB9929381D0 (en) * | 1999-12-10 | 2000-02-09 | Pyrosequencing Ab | A method of assessing the amount of nucleic acid in a sample |
| GB0021977D0 (en) | 2000-09-07 | 2000-10-25 | Pyrosequencing Ab | Method of sequencing DNA |
| GB0103622D0 (en) | 2001-02-14 | 2001-03-28 | Univ Cambridge Tech | Methods for detecting DNA polymerisation |
| JP2002306180A (ja) | 2001-04-16 | 2002-10-22 | Hitachi Ltd | 核酸塩基配列解析法および核酸塩基配列解析試薬キットおよび核酸塩基配列解析装置 |
| GB0111275D0 (en) | 2001-05-09 | 2001-06-27 | Secr Defence | Analytical method and kit |
| EP1415004A4 (en) | 2001-07-03 | 2005-01-19 | Univ Leland Stanford Junior | BIOLUMINESCENCE REGENERATING CIRCULATION (BRC) FOR NUCLEIC ACID QUANTIFICATION |
| US6902921B2 (en) * | 2001-10-30 | 2005-06-07 | 454 Corporation | Sulfurylase-luciferase fusion proteins and thermostable sulfurylase |
| US20040197793A1 (en) | 2002-08-30 | 2004-10-07 | Arjang Hassibi | Methods and apparatus for biomolecule detection, identification, quantification and/or sequencing |
| GB0300802D0 (en) | 2003-01-14 | 2003-02-12 | Murray James A H | Method for detecting DNA polymerisation |
| AR044219A1 (es) | 2003-04-14 | 2005-09-07 | Temasek Life Sciences Lab | Deteccion de transgenes de organismos geneticamente modificados mediante piroluminiscencia |
| JP2008142874A (ja) | 2006-12-13 | 2008-06-26 | Pascal Engineering Corp | 回転テーブルおよびこの回転テーブルを備えた工作機械 |
-
2004
- 2004-07-29 GB GBGB0416944.7A patent/GB0416944D0/en not_active Ceased
-
2005
- 2005-07-29 WO PCT/GB2005/002998 patent/WO2006010948A1/en not_active Ceased
- 2005-07-29 US US11/632,639 patent/US8309308B2/en not_active Expired - Lifetime
- 2005-07-29 AU AU2005266143A patent/AU2005266143B2/en not_active Ceased
- 2005-07-29 EP EP05767491.3A patent/EP1776467B8/en not_active Expired - Lifetime
- 2005-07-29 CN CN2005800254365A patent/CN101035908B/zh not_active Expired - Fee Related
- 2005-07-29 ES ES05767491.3T patent/ES2557158T3/es not_active Expired - Lifetime
- 2005-07-29 JP JP2007523157A patent/JP5055118B2/ja not_active Expired - Fee Related
- 2005-07-29 CA CA2575217A patent/CA2575217C/en not_active Expired - Lifetime
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Daher et al. | Recombinase polymerase amplification for diagnostic applications | |
| KR101098764B1 (ko) | 안정화된 핫스타트 ｐｃｒ용 건조 조성물 | |
| Karsai et al. | Evaluation of a homemade SYBR® Green I reaction mixture for real-time PCR quantification of gene expression | |
| EP2678445B1 (en) | Method for quantifying human dna | |
| JP2008510455A5 (https=) | ||
| Schmerer et al. | Optimized DNA extraction to improve reproducibility of short tandem repeat genotyping with highly degraded DNA as target | |
| EP2261371A3 (en) | Nucleic acid amplification | |
| US9200317B2 (en) | Nucleic acid amplification | |
| CN105087825A (zh) | 常温等温快速检测埃博拉病毒的方法、试剂及引物和探针 | |
| CN1852990B (zh) | 确定样本中核酸数量的方法 | |
| CN101260431A (zh) | 基于滚环扩增技术检测转基因物种的方法 | |
| JP2016019495A (ja) | 核酸増幅法 | |
| JP5055118B2 (ja) | 試料中に存在するテンプレート核酸の量を決定するための方法 | |
| US7989190B2 (en) | Composition for analyzing nucleic acid | |
| US12435360B2 (en) | Reaction condition composition for circularizing oligonucleotide probes | |
| Li et al. | Developmental validation of the EX20+ 4 system | |
| Park et al. | Development and characterization of novel polymorphic microsatellite markers for the Korean freshwater snail Semisulcospira coreana and cross-species amplification using next-generation sequencing | |
| JP2013538585A (ja) | Ｒｔ−ｐｃｒ反応緩衝液中での細胞溶解のための方法 | |
| CN100478453C (zh) | 添加焦磷酸酶的实时pcr | |
| JP2013017427A (ja) | 核酸の増幅検出方法およびキット | |
| JP6174999B2 (ja) | Ｐｃｒ反応緩衝液中での細胞溶解のための方法 | |
| KR101906217B1 (ko) | 돼지 젤라틴 검출 키트 및 이를 이용한 돼지 젤라틴 검출 방법 | |
| JP2017099361A (ja) | 核酸増幅法 | |
| WO2020059792A1 (ja) | 核酸増幅反応用組成物 | |
| JP6852267B2 (ja) | 核酸検出反応液中のプローブの安定化方法 |