JP2008510455A5 - - Google Patents
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- Publication number
- JP2008510455A5 JP2008510455A5 JP2007523157A JP2007523157A JP2008510455A5 JP 2008510455 A5 JP2008510455 A5 JP 2008510455A5 JP 2007523157 A JP2007523157 A JP 2007523157A JP 2007523157 A JP2007523157 A JP 2007523157A JP 2008510455 A5 JP2008510455 A5 JP 2008510455A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- present
- light output
- sample
- template nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000007523 nucleic acids Chemical class 0.000 claims description 52
- 108020004707 nucleic acids Proteins 0.000 claims description 50
- 102000039446 nucleic acids Human genes 0.000 claims description 50
- 238000000034 method Methods 0.000 claims description 44
- 230000003321 amplification Effects 0.000 claims description 21
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 21
- 238000006243 chemical reaction Methods 0.000 claims description 16
- 102000004190 Enzymes Human genes 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 9
- 239000000758 substrate Substances 0.000 claims description 7
- 238000005415 bioluminescence Methods 0.000 claims description 6
- 230000029918 bioluminescence Effects 0.000 claims description 6
- 238000003556 assay Methods 0.000 claims description 5
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 claims description 4
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 claims description 4
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 claims description 4
- 108060001084 Luciferase Proteins 0.000 claims description 4
- 239000005089 Luciferase Substances 0.000 claims description 4
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 claims description 4
- 102000004523 Sulfate Adenylyltransferase Human genes 0.000 claims description 3
- 108010022348 Sulfate adenylyltransferase Proteins 0.000 claims description 3
- 230000002068 genetic effect Effects 0.000 claims description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 2
- 238000012544 monitoring process Methods 0.000 claims description 2
- 230000007423 decrease Effects 0.000 claims 5
- 239000002253 acid Substances 0.000 claims 2
- 239000000872 buffer Substances 0.000 claims 2
- MWMOPIVLTLEUJO-UHFFFAOYSA-N 2-oxopropanoic acid;phosphoric acid Chemical compound OP(O)(O)=O.CC(=O)C(O)=O MWMOPIVLTLEUJO-UHFFFAOYSA-N 0.000 claims 1
- 241000606161 Chlamydia Species 0.000 claims 1
- 101100409013 Mesembryanthemum crystallinum PPD gene Proteins 0.000 claims 1
- 235000010730 Ulex europaeus Nutrition 0.000 claims 1
- 240000003864 Ulex europaeus Species 0.000 claims 1
- 230000001351 cycling effect Effects 0.000 claims 1
- 238000002405 diagnostic procedure Methods 0.000 claims 1
- 201000001371 inclusion conjunctivitis Diseases 0.000 claims 1
- 239000004615 ingredient Substances 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 244000052769 pathogen Species 0.000 claims 1
- 230000001717 pathogenic effect Effects 0.000 claims 1
- 101150063097 ppdK gene Proteins 0.000 claims 1
- 206010044325 trachoma Diseases 0.000 claims 1
- 108020004414 DNA Proteins 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 239000012131 assay buffer Substances 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 1
- 235000011180 diphosphates Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0416944.7 | 2004-07-29 | ||
| GBGB0416944.7A GB0416944D0 (en) | 2004-07-29 | 2004-07-29 | Method for determining the amount of template nucleic acid present in a sample |
| PCT/GB2005/002998 WO2006010948A1 (en) | 2004-07-29 | 2005-07-29 | Method for determining the amount of template nucleic acid present in a sample |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2008510455A JP2008510455A (ja) | 2008-04-10 |
| JP2008510455A5 true JP2008510455A5 (https=) | 2008-09-11 |
| JP5055118B2 JP5055118B2 (ja) | 2012-10-24 |
Family
ID=32947663
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007523157A Expired - Fee Related JP5055118B2 (ja) | 2004-07-29 | 2005-07-29 | 試料中に存在するテンプレート核酸の量を決定するための方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US8309308B2 (https=) |
| EP (1) | EP1776467B8 (https=) |
| JP (1) | JP5055118B2 (https=) |
| CN (1) | CN101035908B (https=) |
| AU (1) | AU2005266143B2 (https=) |
| CA (1) | CA2575217C (https=) |
| ES (1) | ES2557158T3 (https=) |
| GB (1) | GB0416944D0 (https=) |
| WO (1) | WO2006010948A1 (https=) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0517005D0 (en) * | 2005-08-19 | 2005-09-28 | Enigma Diagnostics Ltd | Analytical method and kit |
| JP5520449B2 (ja) * | 2007-03-06 | 2014-06-11 | 株式会社ビー・エム・エル | 核酸の検出方法 |
| JP5570731B2 (ja) * | 2008-02-28 | 2014-08-13 | 旭化成ファーマ株式会社 | ピロリン酸の測定方法 |
| GB0812862D0 (en) | 2008-07-14 | 2008-08-20 | Lumora Ltd | Nucleic acid amplification |
| US9677981B2 (en) | 2013-03-15 | 2017-06-13 | 3M Innovative Properties Company | Sample concentrator and method of use |
| CN106661600B (zh) | 2014-07-16 | 2021-04-06 | 唐恩生物科技股份有限公司 | 用于扩增核酸样品的等温方法 |
| US11559801B2 (en) | 2014-11-03 | 2023-01-24 | Tangen Biosciences, Inc. | Apparatus and method for cell, spore, or virus capture and disruption |
| AU2019235867A1 (en) * | 2018-03-13 | 2020-10-29 | Sarmal, Inc. | Methods for single molecule sequencing |
| US12325883B2 (en) | 2019-01-15 | 2025-06-10 | Neogen Food Safety Us Holdco Corporation | Loop-mediated isothermal amplification primers for Shiga toxin-producing E. coli (STEC) detection |
| US11008627B2 (en) | 2019-08-15 | 2021-05-18 | Talis Biomedical Corporation | Diagnostic system |
| JPWO2021192965A1 (https=) * | 2020-03-26 | 2021-09-30 | ||
| CN115216521B (zh) * | 2022-08-05 | 2025-03-21 | 苏州卫生职业技术学院 | 核酸扩增产物检测试剂及检测方法 |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5498523A (en) | 1988-07-12 | 1996-03-12 | President And Fellows Of Harvard College | DNA sequencing with pyrophosphatase |
| FR2674254B1 (fr) | 1991-03-20 | 1995-10-06 | Univ Reims Champagne Ardenne | Detection non radioactive de la presence d'un acide nucleique determine dans un echantillon biologique. |
| US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| GB9210176D0 (en) | 1992-05-12 | 1992-06-24 | Cemu Bioteknik Ab | Chemical method |
| WO1995025180A1 (en) * | 1994-03-16 | 1995-09-21 | Gen-Probe Incorporated | Isothermal strand displacement nucleic acid amplification |
| WO1996022376A1 (en) | 1995-01-20 | 1996-07-25 | The Secretary Of State For Defence | Mutant luciferases |
| EP0904408A1 (en) | 1996-05-17 | 1999-03-31 | E.I. Du Pont De Nemours And Company | Identification of target bacteria by fluorescence detection of primer directed amplification products |
| EP1033411B1 (en) | 1996-06-04 | 2006-02-22 | University of Utah Research Foundation | Fluorescent donor-acceptor pair |
| GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
| JPH10108679A (ja) | 1996-10-03 | 1998-04-28 | Kikkoman Corp | ピルベートオルトフォスフェートジキナーゼ遺伝子、組み換え体ｄｎａ及びピルベートオルトフォスフェートジキナーゼの製造法 |
| GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
| US20030104372A1 (en) | 1996-12-23 | 2003-06-05 | Pyrosequencing Ab. | Allele specific primer extension |
| WO1998055653A1 (en) * | 1997-06-06 | 1998-12-10 | Nexstar Pharmaceuticals, Inc. | Nucleic acid detection |
| EP2860252A1 (en) | 1997-09-19 | 2015-04-15 | Promega Corporation | Thermostable luciferases and methods of production |
| GB9803382D0 (en) | 1998-02-19 | 1998-04-15 | Secr Defence | Detection system |
| GB9929381D0 (en) | 1999-12-10 | 2000-02-09 | Pyrosequencing Ab | A method of assessing the amount of nucleic acid in a sample |
| GB0021977D0 (en) | 2000-09-07 | 2000-10-25 | Pyrosequencing Ab | Method of sequencing DNA |
| GB0103622D0 (en) | 2001-02-14 | 2001-03-28 | Univ Cambridge Tech | Methods for detecting DNA polymerisation |
| JP2002306180A (ja) | 2001-04-16 | 2002-10-22 | Hitachi Ltd | 核酸塩基配列解析法および核酸塩基配列解析試薬キットおよび核酸塩基配列解析装置 |
| GB0111275D0 (en) * | 2001-05-09 | 2001-06-27 | Secr Defence | Analytical method and kit |
| AU2002367744A1 (en) | 2001-07-03 | 2003-10-27 | The Board Of Trustees Of The Leland Stanford University | Bioluminescence regenerative cycle (brc) for nucleic acid quantification |
| US6902921B2 (en) * | 2001-10-30 | 2005-06-07 | 454 Corporation | Sulfurylase-luciferase fusion proteins and thermostable sulfurylase |
| US20040197793A1 (en) | 2002-08-30 | 2004-10-07 | Arjang Hassibi | Methods and apparatus for biomolecule detection, identification, quantification and/or sequencing |
| GB0300802D0 (en) | 2003-01-14 | 2003-02-12 | Murray James A H | Method for detecting DNA polymerisation |
| EP1616027A4 (en) | 2003-04-14 | 2009-07-22 | Temasek Life Sciences Lab Ltd | DETECTION OF TARGET NUCLEIC ACID BY MEANS OF POLYMERIC ACTION AND ENZYMATIC DETECTION OF UNLOCKED PYROPHOSPHATE |
| JP2008142874A (ja) | 2006-12-13 | 2008-06-26 | Pascal Engineering Corp | 回転テーブルおよびこの回転テーブルを備えた工作機械 |
-
2004
- 2004-07-29 GB GBGB0416944.7A patent/GB0416944D0/en not_active Ceased
-
2005
- 2005-07-29 ES ES05767491.3T patent/ES2557158T3/es not_active Expired - Lifetime
- 2005-07-29 CN CN2005800254365A patent/CN101035908B/zh not_active Expired - Fee Related
- 2005-07-29 US US11/632,639 patent/US8309308B2/en not_active Expired - Lifetime
- 2005-07-29 WO PCT/GB2005/002998 patent/WO2006010948A1/en not_active Ceased
- 2005-07-29 CA CA2575217A patent/CA2575217C/en not_active Expired - Lifetime
- 2005-07-29 EP EP05767491.3A patent/EP1776467B8/en not_active Expired - Lifetime
- 2005-07-29 JP JP2007523157A patent/JP5055118B2/ja not_active Expired - Fee Related
- 2005-07-29 AU AU2005266143A patent/AU2005266143B2/en not_active Ceased
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