FR2674254B1 - Detection non radioactive de la presence d'un acide nucleique determine dans un echantillon biologique. - Google Patents

Detection non radioactive de la presence d'un acide nucleique determine dans un echantillon biologique.

Info

Publication number
FR2674254B1
FR2674254B1 FR9103398A FR9103398A FR2674254B1 FR 2674254 B1 FR2674254 B1 FR 2674254B1 FR 9103398 A FR9103398 A FR 9103398A FR 9103398 A FR9103398 A FR 9103398A FR 2674254 B1 FR2674254 B1 FR 2674254B1
Authority
FR
France
Prior art keywords
nucleic acid
biological sample
radioactive detection
determined nucleic
radioactive
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
FR9103398A
Other languages
English (en)
Other versions
FR2674254A1 (fr
Inventor
Cohen Jacques
Tabary Thierry
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universite de Reims Champagne Ardenne URCA
Original Assignee
Universite de Reims Champagne Ardenne URCA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universite de Reims Champagne Ardenne URCA filed Critical Universite de Reims Champagne Ardenne URCA
Priority to FR9103398A priority Critical patent/FR2674254B1/fr
Priority to PCT/FR1992/000251 priority patent/WO1992016654A1/fr
Priority to AU16460/92A priority patent/AU1646092A/en
Publication of FR2674254A1 publication Critical patent/FR2674254A1/fr
Application granted granted Critical
Publication of FR2674254B1 publication Critical patent/FR2674254B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Urology & Nephrology (AREA)
  • AIDS & HIV (AREA)
  • Inorganic Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
FR9103398A 1991-03-20 1991-03-20 Detection non radioactive de la presence d'un acide nucleique determine dans un echantillon biologique. Expired - Lifetime FR2674254B1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FR9103398A FR2674254B1 (fr) 1991-03-20 1991-03-20 Detection non radioactive de la presence d'un acide nucleique determine dans un echantillon biologique.
PCT/FR1992/000251 WO1992016654A1 (fr) 1991-03-20 1992-03-19 Detection non radioactive de la presence d'un acide nucleique determine dans un echantillon biologique
AU16460/92A AU1646092A (en) 1991-03-20 1992-03-19 Non-radioactive determination of the presence of a given nucleic acid in a biological sample

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9103398A FR2674254B1 (fr) 1991-03-20 1991-03-20 Detection non radioactive de la presence d'un acide nucleique determine dans un echantillon biologique.

Publications (2)

Publication Number Publication Date
FR2674254A1 FR2674254A1 (fr) 1992-09-25
FR2674254B1 true FR2674254B1 (fr) 1995-10-06

Family

ID=9410947

Family Applications (1)

Application Number Title Priority Date Filing Date
FR9103398A Expired - Lifetime FR2674254B1 (fr) 1991-03-20 1991-03-20 Detection non radioactive de la presence d'un acide nucleique determine dans un echantillon biologique.

Country Status (3)

Country Link
AU (1) AU1646092A (fr)
FR (1) FR2674254B1 (fr)
WO (1) WO1992016654A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0630974A3 (fr) * 1993-06-25 1995-11-15 Clinical Diagnostic Syst Procédé et housse d'essai de détection d'orthophosphate inorganique dans l'amplification d'acides nucléiques cibles.
EP0663447B1 (fr) * 1993-12-28 2003-07-09 Eiken Chemical Co., Ltd. Procédé pour la détection de polynucléotides spécifiques
GB9620209D0 (en) * 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
WO1998015655A1 (fr) * 1996-10-07 1998-04-16 The Perkin-Elmer Corporation Reaction d'extension d'amorce utilisant une paire d'enzymes d'un meme substrat pour consommer le pyrophosphate
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
GB0103622D0 (en) * 2001-02-14 2001-03-28 Univ Cambridge Tech Methods for detecting DNA polymerisation
US7141370B2 (en) 2001-07-03 2006-11-28 The Board Of Trustees Of The Leland Stanford Junior University Bioluminescence regenerative cycle (BRC) for nucleic acid quantification
JP4185763B2 (ja) * 2002-12-12 2008-11-26 富士フイルム株式会社 ターゲット核酸の定量方法
JP2004187604A (ja) * 2002-12-12 2004-07-08 Fuji Photo Film Co Ltd 遺伝子発現量の分析方法
WO2004090167A1 (fr) * 2003-04-14 2004-10-21 Temasek Life Sciences Laboratory Limited Detection d'un acide nucleique cible par reaction de la polymerase, et detection enzymatique de pyrophosphate libere
GB0416944D0 (en) 2004-07-29 2004-09-01 Lumora Ltd Method for determining the amount of template nucleic acid present in a sample

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4971903A (en) * 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids
JP2997043B2 (ja) * 1989-04-12 2000-01-11 プレジデント アンド フェロウズ オブ ハーバード カレッジ 改良されたプライマー伸長反応

Also Published As

Publication number Publication date
AU1646092A (en) 1992-10-21
FR2674254A1 (fr) 1992-09-25
WO1992016654A1 (fr) 1992-10-01

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Legal Events

Date Code Title Description
CL Concession to grant licences
ST Notification of lapse

Effective date: 20061130

D3 Decision to revoke the decision of lapse