AR044219A1 - Deteccion de transgenes de organismos geneticamente modificados mediante piroluminiscencia - Google Patents

Deteccion de transgenes de organismos geneticamente modificados mediante piroluminiscencia

Info

Publication number
AR044219A1
AR044219A1 ARP040101250A ARP040101250A AR044219A1 AR 044219 A1 AR044219 A1 AR 044219A1 AR P040101250 A ARP040101250 A AR P040101250A AR P040101250 A ARP040101250 A AR P040101250A AR 044219 A1 AR044219 A1 AR 044219A1
Authority
AR
Argentina
Prior art keywords
nucleic acid
target nucleic
target
sample
replication
Prior art date
Application number
ARP040101250A
Other languages
English (en)
Original Assignee
Temasek Life Sciences Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Temasek Life Sciences Lab filed Critical Temasek Life Sciences Lab
Publication of AR044219A1 publication Critical patent/AR044219A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Abstract

La presente proporciona métodos para identificar la presencia de un ácido nucleico objetivo en una muestra, en donde el ácido nucleico objetivo es replicado, y dicha replicación del objetivo se detecta como el consumo de precursores de desoxinucleótido trifosfato, por lo cual cualquier liberación de PPi indica la incorporación de desoxinucleótido o didesoxinucleótido, y por lo tanto, la replicación del ADN objetivo. Provee equipos y mezclas de reacción utilizados en los métodos de la presente. Reivindicación 1: Un método para identificar la presencia de un ácido nucleico objetivo en una muestra, en donde el ácido nucleico objetivo es replicado, y dicha replicación del objetivo se detecta como el consumo de un precursor de desoxinucleótido trifosfato, en donde el método comprende: agregar un cebador de oligonucleótidos que hibrida al ácido nucleico objetivo de la muestra; someter el ADN de la muestra y cebador a una reacción de la polimerasa en presencia de una mezcla de todos los dNTP necesarios para la replicación del ácido nucleico objetivo, con lo cual los desoxinucleótidos se incorporarán y liberarán pirofosfato (PPi) proporcional a la longitud del producto de extensión de ADN; y detectar de manera enzimática cualquier liberación de PPi; por lo cual cualquier liberación de PPi indica la incorporación de desoxinucleótido o didesoxinucleótido y la presencia del ADN objetivo.
ARP040101250A 2003-04-14 2004-04-14 Deteccion de transgenes de organismos geneticamente modificados mediante piroluminiscencia AR044219A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US46230803P 2003-04-14 2003-04-14

Publications (1)

Publication Number Publication Date
AR044219A1 true AR044219A1 (es) 2005-09-07

Family

ID=33159849

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP040101250A AR044219A1 (es) 2003-04-14 2004-04-14 Deteccion de transgenes de organismos geneticamente modificados mediante piroluminiscencia

Country Status (5)

Country Link
US (1) US20070077561A1 (es)
EP (1) EP1616027A4 (es)
JP (1) JP2006523455A (es)
AR (1) AR044219A1 (es)
WO (1) WO2004090167A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0300802D0 (en) 2003-01-14 2003-02-12 Murray James A H Method for detecting DNA polymerisation
GB0416944D0 (en) 2004-07-29 2004-09-01 Lumora Ltd Method for determining the amount of template nucleic acid present in a sample
WO2007001899A1 (en) * 2005-06-20 2007-01-04 Cargill, Incorporated Method, apparatus and system for quantifying the content of genetically modified material in a sample comprising contaminations
JP2007068450A (ja) * 2005-09-06 2007-03-22 Hitachi Ltd 段階反応を用いたｄｎａ塩基配列決定方法
CN109797195A (zh) * 2019-01-18 2019-05-24 东南大学 基于分析pcr副产物焦磷酸的特异pcr产物快速检测方法及其应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4971903A (en) * 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids
FR2674254B1 (fr) * 1991-03-20 1995-10-06 Univ Reims Champagne Ardenne Detection non radioactive de la presence d'un acide nucleique determine dans un echantillon biologique.
EP0630974A3 (en) * 1993-06-25 1995-11-15 Clinical Diagnostic Syst Method and test kit for the detection of inorganic orthophosphate in the amplification of target nucleic acids.
CA2255673C (en) * 1996-05-17 2000-04-04 Pioneer Hi-Bred International, Inc. Promoter elements conferring root-preferred gene expression
GB9620209D0 (en) * 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) * 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
GB2324865B (en) * 1997-05-02 2001-05-02 William Joseph Harris Materials and methods for detecting amplified nucleic acids
WO1998055653A1 (en) * 1997-06-06 1998-12-10 Nexstar Pharmaceuticals, Inc. Nucleic acid detection
GB9828785D0 (en) * 1998-12-30 1999-02-17 Amersham Pharm Biotech Ab Sequencing systems
WO2001040488A1 (en) * 1999-11-30 2001-06-07 Novartis Ag Increased transgene expression in retroviral vectors having scaffold attachment region
AU2001293044B2 (en) * 2000-09-29 2006-07-13 Monsanto Technology Llc Glyphosate tolerant wheat plant 33391 and compositions and methods for detection thereof
GB0103622D0 (en) * 2001-02-14 2001-03-28 Univ Cambridge Tech Methods for detecting DNA polymerisation
US7141370B2 (en) * 2001-07-03 2006-11-28 The Board Of Trustees Of The Leland Stanford Junior University Bioluminescence regenerative cycle (BRC) for nucleic acid quantification

Also Published As

Publication number Publication date
WO2004090167A1 (en) 2004-10-21
JP2006523455A (ja) 2006-10-19
EP1616027A4 (en) 2009-07-22
EP1616027A1 (en) 2006-01-18
US20070077561A1 (en) 2007-04-05

Similar Documents

Publication Publication Date Title
JP7104730B2 (ja) 連続的な増幅反応のための方法および装置
DK1421213T3 (da) Mærkede nukleosidpolyphosphater
US8119352B2 (en) Multi-stage amplification reactions by control of sequence replication times
CN104540964A (zh) 使用序列标签的单细胞分析
KR20070073935A (ko) 폐쇄 시스템의 다단계 핵산 증폭 반응
ATE549419T1 (de) Verfahren und zusammensetzungen für die universelle grössenspezifische polymerasekettenreaktion
ES2531055T3 (es) Cebadores de polinucleótidos para detectar mutaciones de PIK3CA
KR910006497A (ko) 프라이머 3' 말단에서의 프라이머-표적 오합(mismatch)을 극복한 진단 및 증폭방법
WO2006138183A3 (en) Multiplexed polymerase chain reaction for genetic sequence analysis
US20080286772A1 (en) Method for Direct Amplification from Crude Nucleic Acid Samples
AR090558A1 (es) Evento de maiz dp-004114-3 y metodos para su deteccion
AR044219A1 (es) Deteccion de transgenes de organismos geneticamente modificados mediante piroluminiscencia
ATE533847T1 (de) Verfahren für den nachweis und die quantifizierung einer endogenen dna-sequenz aus weizen
CN104263831A (zh) 一种简便快速的碱性磷酸酶活性测定方法
US11332780B2 (en) Simplified polynucleotide sequence detection method
DE602008004702D1 (de) Verfahren zur Amplifikation von Nukleinsäure
WO2011104694A3 (en) Detection of braf v600e mutation by allele specific real time quantitative pcr (as-qpcr) using locked nucleic acids primers and beacon probes
US11072822B2 (en) RNA amplification method, RNA detection method and assay kit
DE50213026D1 (de) Tifikation in einer probe
JP2006075167A (ja) ピロホスファターゼの添加を伴うリアルタイムｐｃｒ
KR101835006B1 (ko) 중합효소 연쇄 반응의 효율 및 감도를 증가시키는 방법 및 이를 위한 조성물
JP6565147B2 (ja) マルチプレックスｐｃｒ法
JP6728657B2 (ja) 核酸増幅法
WO2022092013A1 (ja) 複数の核酸プローブからなる核酸プローブセット
EA201990928A1 (ru) Амплификация нуклеиновых кислот с использованием экзонуклеазы и замещения цепи

Legal Events

Date Code Title Description
FA Abandonment or withdrawal