CN101035908B - 确定样本中模板核酸数量的方法 - Google Patents

确定样本中模板核酸数量的方法 Download PDF

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Publication number
CN101035908B
CN101035908B CN2005800254365A CN200580025436A CN101035908B CN 101035908 B CN101035908 B CN 101035908B CN 2005800254365 A CN2005800254365 A CN 2005800254365A CN 200580025436 A CN200580025436 A CN 200580025436A CN 101035908 B CN101035908 B CN 101035908B
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nucleic acid
sample
reaction
time
amplification reaction
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Chinese (zh)
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CN101035908A (zh
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劳伦斯·C·第希
詹姆斯·A·H·默里
奥尔加·甘德尔曼
维多利亚·L·丘奇
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Lumora Ltd
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Lumora Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
CN2005800254365A 2004-07-29 2005-07-29 确定样本中模板核酸数量的方法 Expired - Fee Related CN101035908B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0416944.7 2004-07-29
GBGB0416944.7A GB0416944D0 (en) 2004-07-29 2004-07-29 Method for determining the amount of template nucleic acid present in a sample
PCT/GB2005/002998 WO2006010948A1 (en) 2004-07-29 2005-07-29 Method for determining the amount of template nucleic acid present in a sample

Publications (2)

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CN101035908A CN101035908A (zh) 2007-09-12
CN101035908B true CN101035908B (zh) 2011-11-16

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CN2005800254365A Expired - Fee Related CN101035908B (zh) 2004-07-29 2005-07-29 确定样本中模板核酸数量的方法

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US (1) US8309308B2 (https=)
EP (1) EP1776467B8 (https=)
JP (1) JP5055118B2 (https=)
CN (1) CN101035908B (https=)
AU (1) AU2005266143B2 (https=)
CA (1) CA2575217C (https=)
ES (1) ES2557158T3 (https=)
GB (1) GB0416944D0 (https=)
WO (1) WO2006010948A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0517005D0 (en) * 2005-08-19 2005-09-28 Enigma Diagnostics Ltd Analytical method and kit
JP5520449B2 (ja) * 2007-03-06 2014-06-11 株式会社ビー・エム・エル 核酸の検出方法
JP5570731B2 (ja) * 2008-02-28 2014-08-13 旭化成ファーマ株式会社 ピロリン酸の測定方法
GB0812862D0 (en) 2008-07-14 2008-08-20 Lumora Ltd Nucleic acid amplification
US9677981B2 (en) 2013-03-15 2017-06-13 3M Innovative Properties Company Sample concentrator and method of use
CN106661600B (zh) 2014-07-16 2021-04-06 唐恩生物科技股份有限公司 用于扩增核酸样品的等温方法
US11559801B2 (en) 2014-11-03 2023-01-24 Tangen Biosciences, Inc. Apparatus and method for cell, spore, or virus capture and disruption
AU2019235867A1 (en) * 2018-03-13 2020-10-29 Sarmal, Inc. Methods for single molecule sequencing
US12325883B2 (en) 2019-01-15 2025-06-10 Neogen Food Safety Us Holdco Corporation Loop-mediated isothermal amplification primers for Shiga toxin-producing E. coli (STEC) detection
US11008627B2 (en) 2019-08-15 2021-05-18 Talis Biomedical Corporation Diagnostic system
JPWO2021192965A1 (https=) * 2020-03-26 2021-09-30
CN115216521B (zh) * 2022-08-05 2025-03-21 苏州卫生职业技术学院 核酸扩增产物检测试剂及检测方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998055653A1 (en) * 1997-06-06 1998-12-10 Nexstar Pharmaceuticals, Inc. Nucleic acid detection
CN1297488A (zh) * 1998-02-19 2001-05-30 英国国防部 用pcr检测目标核酸的方法
WO2001042496A2 (en) * 1999-12-10 2001-06-14 Pyrosequencing Ab A method of assessing the amount of nucleic acid in a sample
WO2002090586A2 (en) * 2001-05-09 2002-11-14 The Secretary Of State For Defence Analytical method and kit

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5498523A (en) 1988-07-12 1996-03-12 President And Fellows Of Harvard College DNA sequencing with pyrophosphatase
FR2674254B1 (fr) 1991-03-20 1995-10-06 Univ Reims Champagne Ardenne Detection non radioactive de la presence d'un acide nucleique determine dans un echantillon biologique.
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
GB9210176D0 (en) 1992-05-12 1992-06-24 Cemu Bioteknik Ab Chemical method
WO1995025180A1 (en) * 1994-03-16 1995-09-21 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
WO1996022376A1 (en) 1995-01-20 1996-07-25 The Secretary Of State For Defence Mutant luciferases
EP0904408A1 (en) 1996-05-17 1999-03-31 E.I. Du Pont De Nemours And Company Identification of target bacteria by fluorescence detection of primer directed amplification products
EP1033411B1 (en) 1996-06-04 2006-02-22 University of Utah Research Foundation Fluorescent donor-acceptor pair
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
JPH10108679A (ja) 1996-10-03 1998-04-28 Kikkoman Corp ピルベートオルトフォスフェートジキナーゼ遺伝子、組み換え体ｄｎａ及びピルベートオルトフォスフェートジキナーゼの製造法
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US20030104372A1 (en) 1996-12-23 2003-06-05 Pyrosequencing Ab. Allele specific primer extension
EP2860252A1 (en) 1997-09-19 2015-04-15 Promega Corporation Thermostable luciferases and methods of production
GB0021977D0 (en) 2000-09-07 2000-10-25 Pyrosequencing Ab Method of sequencing DNA
GB0103622D0 (en) 2001-02-14 2001-03-28 Univ Cambridge Tech Methods for detecting DNA polymerisation
JP2002306180A (ja) 2001-04-16 2002-10-22 Hitachi Ltd 核酸塩基配列解析法および核酸塩基配列解析試薬キットおよび核酸塩基配列解析装置
AU2002367744A1 (en) 2001-07-03 2003-10-27 The Board Of Trustees Of The Leland Stanford University Bioluminescence regenerative cycle (brc) for nucleic acid quantification
US6902921B2 (en) * 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US20040197793A1 (en) 2002-08-30 2004-10-07 Arjang Hassibi Methods and apparatus for biomolecule detection, identification, quantification and/or sequencing
GB0300802D0 (en) 2003-01-14 2003-02-12 Murray James A H Method for detecting DNA polymerisation
EP1616027A4 (en) 2003-04-14 2009-07-22 Temasek Life Sciences Lab Ltd DETECTION OF TARGET NUCLEIC ACID BY MEANS OF POLYMERIC ACTION AND ENZYMATIC DETECTION OF UNLOCKED PYROPHOSPHATE
JP2008142874A (ja) 2006-12-13 2008-06-26 Pascal Engineering Corp 回転テーブルおよびこの回転テーブルを備えた工作機械

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998055653A1 (en) * 1997-06-06 1998-12-10 Nexstar Pharmaceuticals, Inc. Nucleic acid detection
CN1297488A (zh) * 1998-02-19 2001-05-30 英国国防部 用pcr检测目标核酸的方法
WO2001042496A2 (en) * 1999-12-10 2001-06-14 Pyrosequencing Ab A method of assessing the amount of nucleic acid in a sample
WO2002090586A2 (en) * 2001-05-09 2002-11-14 The Secretary Of State For Defence Analytical method and kit

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Arakawa H, et al.A new assay for determining pyrophosphate using pyruvatephosphate dikinase and its application to DNA analysis.Luminescence19.2004,19第132页. *
Arakawa H, et al.Detection of cariogenic bacteria genes by a combination ofallele-specific polymerase chain reactions and a novelbioluminescent pyrophosphate assay.Analytical Biochemistry333.2004,333第296-298页摘要,材料与方法. *
Maeda M.Determination of biological substances using bioluminescentreaction based on luciferin-luciferase.The Japanese Journal of Clinical Pathology52 7.2004,52(7),第595-603页摘要,第I-II部分,图1-6.
Maeda M.Determination of biological substances using bioluminescentreaction based on luciferin-luciferase.The Japanese Journal of Clinical Pathology52 7.2004,52(7),第595-603页摘要,第I-II部分,图1-6. *

Also Published As

Publication number Publication date
AU2005266143A1 (en) 2006-02-02
EP1776467B8 (en) 2015-10-28
US8309308B2 (en) 2012-11-13
CA2575217A1 (en) 2006-02-02
EP1776467A1 (en) 2007-04-25
JP2008510455A (ja) 2008-04-10
CN101035908A (zh) 2007-09-12
WO2006010948A1 (en) 2006-02-02
GB0416944D0 (en) 2004-09-01
EP1776467B1 (en) 2015-09-23
AU2005266143B2 (en) 2011-06-16
JP5055118B2 (ja) 2012-10-24
ES2557158T3 (es) 2016-01-22
US20080118921A1 (en) 2008-05-22
CA2575217C (en) 2016-10-11

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