JP2001502908A - ニトリラーゼを用いる2―ヒドロキシ―4―メチルチオ酪酸の製造方法 - Google Patents
ニトリラーゼを用いる2―ヒドロキシ―4―メチルチオ酪酸の製造方法Info
- Publication number
- JP2001502908A JP2001502908A JP10520112A JP52011298A JP2001502908A JP 2001502908 A JP2001502908 A JP 2001502908A JP 10520112 A JP10520112 A JP 10520112A JP 52011298 A JP52011298 A JP 52011298A JP 2001502908 A JP2001502908 A JP 2001502908A
- Authority
- JP
- Japan
- Prior art keywords
- hydroxy
- acid
- nitrilase
- prpa
- plasmid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108010033272 Nitrilase Proteins 0.000 title claims abstract description 58
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1. a)第一段階として、ニトリラーゼ活性を有する生物学的材料を調製し 、 b)第二段階として、それを固定化し、 c)第三段階として、2−ヒドロキシ−4−メチルチオ酪酸のアンモニ ウム塩を得るために、このように固定化された生物学的材料に2−ヒドロキシ− 4−メチルチオブチロニトリルをさらし、 d)第四段階として、場合により、段階c)で得られた塩を対応する酸 に転化してもよく、そして e)第五段階として、段階c)またはd)で得られた生成物を濃縮する ことを特徴とする、2−ヒドロキシ−4−メチルチオブチロニトリルの酵素加水 分解による2−ヒドロキシ−4−メチルチオ酪酸及び/または2−ヒドロキシ− 4−メチルチオ酪酸のアンモニウム塩の製造方法。 2. ニトリラーゼ活性がアルカリゲネス、好ましくはファエカリスのニトリ ラーゼから得られることを特徴とする、請求の範囲1の方法。 3. 宿主微生物において発現されるニトリラーゼをコードする遺伝情報を用 いることを特徴とする、請求の範囲1または請求の範囲2の方法。 4. 宿主微生物がエシェリキア・コリまたは属バシラス、コリネバクテリア 、ストレプトマイセス、サッカロマイセス、クルイベロマイセス、ペニシリウム 及びアスペルギルスのメンバーから選択されることを特徴とする、請求の範囲1 ないし3のいずれかの方法。 5. ニトリラーゼ活性が番号CBS 998−96でCBSに寄託 されたプラスミドpRPA−BCAT3中にクローン化された遺伝子によりコー ドされるニトリラーゼから得られることを特徴とする、請求の範囲1の方法。 6. ニトリラーゼがシャペロンタンパク質と同時発現されることを特徴とす る、請求の範囲1ないし5のいずれかの方法。 7. シャペロンタンパク質がエシェリキア・コリのGroESLであること を特徴とする、請求の範囲5の方法。 8. 段階a)の生物学的材料が固体担体上に固定されることを特徴とする、 先行する請求の範囲のいずれかの方法。 9. 固体担体が1μmないし500μm、好ましくは10μmないし200 μmの間の粒度を有し、そして生物学的材料の重量で0.01ないし200%、 好ましくは10ないし100%の量でそれを添加することを特徴とする、請求の 範囲8の方法。 10. 固体担体が ‐イオン交換樹脂、 ‐アルミナ、 ‐合成シリカまたは珪藻土及びシリカゲル、 ‐ゼオライト、 ‐木炭、 ‐ある程度水溶性のタンパク質、並びに ‐多糖 から選択されることを特徴とする、請求の範囲9の方法。 11. 固体担体がグルテンであることを特徴とする、請求の範囲10の方法 。 12. 生物学的材料及び担体を架橋及び/または不溶化するために1つまた はそれより多い化学薬剤を用いることを特徴とする、請求の範囲8の方法。 13. 化学薬剤が ‐ポリアゼチジンポリマー、 ‐ポリエチレンイミンポリマー、 ‐ポリアミドポリマー、 ‐イソシアネートポリマー、 ‐アルギン酸ゲル、 ‐k−カラギーナンゲル、 ‐アミン、 ‐アルデヒド、 ‐カルボン酸、及び ‐イソシアネート から選択されることを特徴とする、請求の範囲12の方法。 14. 化学薬剤の存在下で生物学的材料及び担体を混合してペーストを得、 それを押出し、次に乾燥させることを特徴とする、請求の範囲10ないし13の 方法。 15. 生物学的材料及び化学薬剤を混合し、次に薄層の形態で担体上に置く ことを特徴とする、請求の範囲10ないし13の方法。 16. 少なくとも60%のHMTBA、好ましくは少なくとも80%のHM TBAを含有し、残りがHMTBSであるHMTBS及びHMTBAの混合物を 対応するアンモニウム塩の電気透析解離により得ることを特徴とする、先行する 請求の範囲のいずれかの方法。 17. 3区画を有する両極性膜を用いた電気透析器において解離を実施する ことを特徴とする、請求の範囲16の方法。 18. 2区画を有する両極性膜を用いた電気透析器において解離を実施する ことを特徴とする、請求の範囲16の方法。 19. アンモニウム塩溶液を加熱することによりHMTBS及びHMTBA の混合物を得ることを特徴とする、請求の範囲1ないし15のいずれかの方法。 20. 遊離した水酸化アンモニウムを蒸留し、濃縮し、そしてHCN合成に 再循環させることを特徴とする、請求の範囲16ないし19の方法。 21. HMTBA/(HMTBA+HMTBS)重量比が5ないし99.9 %、好ましくは10ないし50%であるHMTBA及びHMTBSの混合物から なる、請求の範囲16ないし19により得られる水溶液。 22. ‐ニトリラーゼ活性を有する固定化された酵素またはニトリラーゼ活 性を有する酵素を発現する固定された宿主微生物を充填した1つまたはそれより 多い反応器(3、4)、 ‐場合により、1つまたはそれより多い電気透析手段(9)、 ‐最終生成物の濃縮のための手段(6、12) を含んでなる本発明の方法を実施するためのプラント。 23. 番号CBS 998−96でCBSに寄託されたプラスミドpRPA −BCAT3。
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FR96/13077 | 1996-10-25 | ||
FR9613077A FR2755143B1 (fr) | 1996-10-25 | 1996-10-25 | Procede de preparation de l'acide 2-hydroxy-4-methylthio-butyrique par utilisation d'une nitrilase |
PCT/FR1997/001913 WO1998018941A1 (fr) | 1996-10-25 | 1997-10-24 | Procede de preparation de l'acide 2-hydroxy 4-methylthio butyrique par utilisation d'une nitrilase |
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EP (2) | EP1411126B1 (ja) |
JP (1) | JP4350801B2 (ja) |
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IL (3) | IL129395A0 (ja) |
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PL (1) | PL190773B1 (ja) |
PT (2) | PT1411126E (ja) |
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JP2008022706A (ja) * | 2006-07-18 | 2008-02-07 | Gifu Univ | ニトリラーゼおよびニトリラーゼを用いるカルボン酸の製造方法 |
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JPH10179183A (ja) * | 1996-12-20 | 1998-07-07 | Daicel Chem Ind Ltd | カルボン酸の製造方法 |
AU6029998A (en) | 1997-01-17 | 1998-08-07 | Regents Of The University Of Minnesota | Dna molecules and protein displaying improved triazine compound degrading ability |
FR2780416B1 (fr) * | 1998-06-10 | 2002-12-20 | Rhone Poulenc Nutrition Animal | Procede industriel de production de proteines heterologues chez e. coli et souches utiles pour le procede |
FR2787121B1 (fr) * | 1998-12-11 | 2003-09-12 | Aventis Cropscience Sa | Nouvelle methode d'isolement et de selection de genes codant pour des enzymes, et milieu de culture approprie |
FR2791668B1 (fr) * | 1999-04-02 | 2001-05-04 | Rhone Poulenc Animal Nutrition | Procede de preparation d' beta-hydroxy acides |
US6368804B1 (en) * | 1999-07-12 | 2002-04-09 | E. I. Du Pont De Nemours & Company | Method for stabilizing nitrilase activity and preserving microbial cells |
FR2800749B1 (fr) * | 1999-11-08 | 2004-07-16 | Rhone Poulenc Animal Nutrition | Procede de modulation de la selective des nitrilases, nouvelles nitrilases obtenues par ce procede et leur utilisation |
US6673582B2 (en) | 2000-01-25 | 2004-01-06 | Regents Of The University Of Minnesota | Microbes and methods for remediation |
EP1167521A1 (en) * | 2000-06-30 | 2002-01-02 | Aventis Animal Nutrition S.A. | Coated enzyme-containing catalyst |
FR2824823A1 (fr) * | 2001-05-21 | 2002-11-22 | Aventis Animal Nutrition Sa | Procede de preparation d'acides carboxyliques aliphatiques par hydrolyse enzymatique de composes nitriles aliphatiques |
JP5143838B2 (ja) * | 2007-07-25 | 2013-02-13 | 日本曹達株式会社 | α−ヒドロキシ酸アンモニウム塩からのα−ヒドロキシ酸の製造方法 |
EP2338987A1 (en) | 2009-11-16 | 2011-06-29 | ZYRUS Beteiligungsgesellschaft mbH & Co. Patente I KG | Whole cell biocatalyst |
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CN103933861B (zh) * | 2013-08-02 | 2017-04-26 | 浙江赛特膜技术有限公司 | 一种双极膜电渗析制备蛋氨酸和氢氧化钠的方法 |
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