JP2000515389A - ジカルボン酸の生産方法 - Google Patents
ジカルボン酸の生産方法Info
- Publication number
- JP2000515389A JP2000515389A JP10533141A JP53314198A JP2000515389A JP 2000515389 A JP2000515389 A JP 2000515389A JP 10533141 A JP10533141 A JP 10533141A JP 53314198 A JP53314198 A JP 53314198A JP 2000515389 A JP2000515389 A JP 2000515389A
- Authority
- JP
- Japan
- Prior art keywords
- organism
- acid
- carbon source
- succinic acid
- anaerobic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 57
- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 title claims description 7
- 238000000034 method Methods 0.000 claims abstract description 45
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 28
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 28
- 239000012298 atmosphere Substances 0.000 claims abstract description 27
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- 230000001965 increasing effect Effects 0.000 claims abstract description 16
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 14
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- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 claims description 151
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 81
- 239000001384 succinic acid Substances 0.000 claims description 71
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 claims description 48
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 43
- 235000011090 malic acid Nutrition 0.000 claims description 42
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 39
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- 241000588724 Escherichia coli Species 0.000 claims description 33
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 31
- 239000001530 fumaric acid Substances 0.000 claims description 24
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims description 24
- 101710104378 Putative malate oxidoreductase [NAD] Proteins 0.000 claims description 17
- 108090000790 Enzymes Proteins 0.000 claims description 12
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- 102000004190 Enzymes Human genes 0.000 claims description 10
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 claims description 10
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- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 32
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- 238000009825 accumulation Methods 0.000 description 4
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- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 4
- 239000004310 lactic acid Substances 0.000 description 4
- 235000014655 lactic acid Nutrition 0.000 description 4
- 101150052159 maeA gene Proteins 0.000 description 4
- ZLNQQNXFFQJAID-UHFFFAOYSA-L magnesium carbonate Chemical compound [Mg+2].[O-]C([O-])=O ZLNQQNXFFQJAID-UHFFFAOYSA-L 0.000 description 4
- 239000001095 magnesium carbonate Substances 0.000 description 4
- 229910000021 magnesium carbonate Inorganic materials 0.000 description 4
- 229940049920 malate Drugs 0.000 description 4
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 4
- 230000009466 transformation Effects 0.000 description 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 4
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 3
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- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 2
- YEJRWHAVMIAJKC-UHFFFAOYSA-N 4-Butyrolactone Chemical compound O=C1CCCO1 YEJRWHAVMIAJKC-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
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- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
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- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1. カルボン酸の生産方法であって、 a)炭素源を有する培地にバイオマスを有するカルボン酸生産生物を接種するこ と; b)該生物を好気的雰囲気下インキュベートし、該生物の急速増殖を促進し該生 物のバイオマスを増加させること; c)酸素を制御しながら供給し、好気的雰囲気を維持すること; d)該生物に炭素源を有する溶液を制御しながら与え、該培地の炭素源の濃度を 約0.5g/L〜約1g/Lに維持すること; e)好気的雰囲気から酸素を奪い、嫌気的雰囲気にし該生物に嫌気的代謝をさせ ること; f)該生物に炭素源を有する溶液を制御しながら与え、培地の炭素源の濃度を1 g/L以上に維持すること;及び g)該生物の嫌気的代謝を用い、炭素源をカルボン酸に転換すること; の各工程を含む該方法。 2. 該生物が大腸菌及び乳酸桿菌からなる群から選択される請求項1に記載の 方法。 3. 該生物は耐浸透圧性であり、それによって該生物は、該生物の代謝の阻害 無くして有機酸を生産可能である請求項1に記載の方法。 4. 大腸菌はAFP−111である請求項2に記載の方法。 5. 該生物は、ピルビン酸ギ酸リアーゼ及び乳酸脱水素酵素の遺伝子欠損の親 生物から由来の大腸菌である請求項2に記載の方法。 6. 該生物がピルビン酸をジカルボン酸に転換するのを可能とする酵素を発現 するように、該生物が遺伝子操作されている請求項1に記載の方法。 7. 該酵素はリンゴ酸酵素である請求項6に記載の方法。 8. 嫌気的代謝を用いる生物は、コハク酸、酢酸、及びエタノールを生産し、 コハク酸が主産物である請求項7に記載の方法。 9. 嫌気的代謝を用いる生物は、リンゴ酸、酢酸、及びエタノールを生産し、 リンゴ酸が主産物である請求項7に記載の方法。 10. 嫌気的代謝を用いる生物は、フマル酸、酢酸、及びエタノールを生産し 、フマル酸が主産物である請求項7に記載の方法。 11. カルボン酸の生産方法であって、 a)炭素源を有する培地にバイオマスを有するカルボン酸生産生物を接種するこ と; b)該生物を維持されたpH値と好気的雰囲気を有する環境中でインキュベート し、該生物の急速増殖を促進し該生物のバイオマスを増加させること; c)酸素を制御しながら供給し、好気的雰囲気を維持すること; d)該生物に炭素源を有する溶液を制御しながら与え、該培地の炭素源の濃度を 約0.5g/L〜約1g/Lに維持すること; e)増加したバイオマスを有する該生物を嫌気的雰囲気を有する生産発酵槽へ移 し、該生物に嫌気的代謝をさせること; f)該生物に炭素源を有する溶液を制御しながら与え、生産発酵槽の炭素源の濃 度を1g/L以上に維持すること;及び g)該生物の嫌気的代謝を用い、炭素源をカルボン酸に転換すること; の各工程を含む該方法。 12. 該生物が大腸菌及び乳酸桿菌からなる群から選択される請求項11に記 載の方法。 13. 該生物は耐浸透圧性であり、それによって該生物は、該生物の代謝の阻 害無くして有機酸を生産可能である請求項11に記載の方法。 14. 大腸菌はAFP−111である請求項12に記載の方法。 15. 該生物は、ピルビン酸ギ酸リアーゼ及び乳酸脱水素酵素の遺伝子欠損の 親生物から由来の大腸菌である請求項12に記載の方法。 16. 該生物がピルビン酸をジカルボン酸に転換するのを可能とする酵素を発 現するように、該生物が遺伝子操作されている請求項11に記載の方法。 17. 該酵素はリンゴ酸酵素である請求項16に記載の方法。 18. 嫌気的代謝を用いる生物は、コハク酸、酢酸、及びエタノールを生産し 、コハク酸が主産物である請求項17に記載の方法。 19. 嫌気的代謝を用いる生物は、リンゴ酸、酢酸、及びエタノールを生産し 、リンゴ酸が主産物である請求項17に記載の方法。 20. 嫌気的雰囲気を用いる生物は、フマル酸、酢酸、及びエタノールを生産 し、フマル酸が主産物である請求項17に記載の方法。
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US08/792,655 | 1997-01-31 | ||
US08/792,655 US5869301A (en) | 1995-11-02 | 1997-01-31 | Method for the production of dicarboxylic acids |
PCT/US1998/001877 WO1998033930A1 (en) | 1997-01-31 | 1998-01-30 | A method for the production of dicarboxylic acids |
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JP3681404B2 JP3681404B2 (ja) | 2005-08-10 |
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JP53314198A Expired - Fee Related JP3681404B2 (ja) | 1997-01-31 | 1998-01-30 | ジカルボン酸の生産方法 |
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US (1) | US5869301A (ja) |
EP (1) | EP1012323B1 (ja) |
JP (1) | JP3681404B2 (ja) |
CN (1) | CN1121500C (ja) |
AT (1) | ATE360084T1 (ja) |
AU (1) | AU724274B2 (ja) |
BR (1) | BR9806822B1 (ja) |
CA (1) | CA2278892C (ja) |
DE (1) | DE69837607T2 (ja) |
DK (1) | DK1012323T3 (ja) |
ES (1) | ES2285757T3 (ja) |
HU (1) | HU227509B1 (ja) |
PT (1) | PT1012323E (ja) |
WO (1) | WO1998033930A1 (ja) |
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JP2005295998A (ja) * | 2003-11-07 | 2005-10-27 | Mitsubishi Chemicals Corp | 有機酸アンモニウム溶液の製造方法 |
JP4537862B2 (ja) * | 2005-01-18 | 2010-09-08 | 財団法人地球環境産業技術研究機構 | 好気性細菌による高効率な有機酸の製造方法 |
JP2006197821A (ja) * | 2005-01-18 | 2006-08-03 | Research Institute Of Innovative Technology For The Earth | 好気性細菌による高効率な有機酸の製造方法 |
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JP2007197654A (ja) * | 2005-04-22 | 2007-08-09 | Mitsubishi Chemicals Corp | ポリエステル及びその製造方法 |
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Also Published As
Publication number | Publication date |
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EP1012323B1 (en) | 2007-04-18 |
CN1121500C (zh) | 2003-09-17 |
AU724274B2 (en) | 2000-09-14 |
HUP0001997A2 (hu) | 2000-10-28 |
EP1012323A4 (en) | 2002-01-16 |
CA2278892A1 (en) | 1998-08-06 |
AU6052398A (en) | 1998-08-25 |
CA2278892C (en) | 2009-02-24 |
DE69837607T2 (de) | 2007-12-27 |
EP1012323A1 (en) | 2000-06-28 |
US5869301A (en) | 1999-02-09 |
CN1246155A (zh) | 2000-03-01 |
ES2285757T3 (es) | 2007-11-16 |
JP3681404B2 (ja) | 2005-08-10 |
PT1012323E (pt) | 2007-05-31 |
BR9806822A (pt) | 2000-05-09 |
ATE360084T1 (de) | 2007-05-15 |
HU227509B1 (en) | 2011-07-28 |
BR9806822B1 (pt) | 2009-05-05 |
DK1012323T3 (da) | 2007-06-18 |
WO1998033930A1 (en) | 1998-08-06 |
DE69837607D1 (de) | 2007-05-31 |
HUP0001997A3 (en) | 2002-01-28 |
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