IL70474A - Methods of purification and reactivation of precipitated heterologous proteins - Google Patents

Methods of purification and reactivation of precipitated heterologous proteins

Info

Publication number
IL70474A
IL70474A IL70474A IL7047483A IL70474A IL 70474 A IL70474 A IL 70474A IL 70474 A IL70474 A IL 70474A IL 7047483 A IL7047483 A IL 7047483A IL 70474 A IL70474 A IL 70474A
Authority
IL
Israel
Prior art keywords
reactivation
purification
methods
heterologous proteins
precipitated
Prior art date
Application number
IL70474A
Other languages
English (en)
Other versions
IL70474A0 (en
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27575443&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=IL70474(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of IL70474A0 publication Critical patent/IL70474A0/xx
Publication of IL70474A publication Critical patent/IL70474A/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Enzymes And Modification Thereof (AREA)
IL70474A 1982-12-22 1983-12-18 Methods of purification and reactivation of precipitated heterologous proteins IL70474A (en)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US45235682A 1982-12-22 1982-12-22
US45234482A 1982-12-22 1982-12-22
US45235782A 1982-12-22 1982-12-22
US45225282A 1982-12-22 1982-12-22
US45218782A 1982-12-22 1982-12-22
US45236382A 1982-12-22 1982-12-22
US45225382A 1982-12-22 1982-12-22
US45235582A 1982-12-22 1982-12-22

Publications (2)

Publication Number Publication Date
IL70474A0 IL70474A0 (en) 1984-03-30
IL70474A true IL70474A (en) 1989-09-28

Family

ID=27575443

Family Applications (1)

Application Number Title Priority Date Filing Date
IL70474A IL70474A (en) 1982-12-22 1983-12-18 Methods of purification and reactivation of precipitated heterologous proteins

Country Status (12)

Country Link
EP (1) EP0114506B1 (en, 2012)
JP (1) JP2575604B2 (en, 2012)
AU (1) AU568109B2 (en, 2012)
BR (1) BR8307086A (en, 2012)
CA (1) CA1219234A (en, 2012)
DE (1) DE3380850D1 (en, 2012)
GR (1) GR79124B (en, 2012)
HU (1) HU197357B (en, 2012)
IL (1) IL70474A (en, 2012)
MX (1) MX166885B (en, 2012)
NZ (1) NZ206615A (en, 2012)
PT (1) PT77866B (en, 2012)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USD1086051S1 (en) * 2022-09-30 2025-07-29 Maxcyte, Inc. Electroporation gasket

Families Citing this family (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4342832A (en) * 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
US4476049A (en) * 1983-09-20 1984-10-09 Hoffmann-La Roche Inc. Method for the extraction of immune interferon
JPH064680B2 (ja) * 1985-01-09 1994-01-19 武田薬品工業株式会社 高濃度ヒトγ型インターフェロンフラグメント水溶液の製造法
JPS60155137A (ja) * 1984-01-23 1985-08-15 Takeda Chem Ind Ltd 高濃度ヒトγ型インタ−フエロン水溶液の製造法
US4530787A (en) * 1984-03-28 1985-07-23 Cetus Corporation Controlled oxidation of microbially produced cysteine-containing proteins
JPS60258129A (ja) * 1984-06-05 1985-12-20 Kyowa Hakko Kogyo Co Ltd インタ−フエロンの活性化法
ATE64956T1 (de) * 1984-06-14 1991-07-15 Ciba Geigy Ag Verfahren zur herstellung von thrombininhibitoren.
CA1267615A (en) * 1984-08-27 1990-04-10 Dan Hadary Method for recovering purified growth hormones from genetically engineered microorganisms
US5871956A (en) * 1984-12-06 1999-02-16 Amgen Inc. Recombinant methods for production of serine inhibitors and DNA sequences useful for same
US6291662B1 (en) 1984-12-05 2001-09-18 Amgen Inc. Recombinant methods for production of serine protease inhibitors and DNA sequences
US6132990A (en) * 1984-12-06 2000-10-17 Amgen Boulder Inc. Recombinant methods for production of serine protease inhibitors and DNA sequences useful for same
IL95223A (en) * 1984-12-06 1992-08-18 Synergen Biolog Inc Dna sequence for production of serine protease inhibitors
US5900400A (en) * 1984-12-06 1999-05-04 Amgen Inc. Serine protease inhibitor analogs
WO1986004608A1 (en) * 1985-02-05 1986-08-14 Synergen Biologicals, Inc. Metalloproteinase inhibitor sequence recombinant vector system for using same and recombinant-dna method for the manufacture of same
US4731440A (en) * 1985-02-22 1988-03-15 Monsanto Company Method of somatotropin solubilization using dimethylsulfone and naturation
US4861868A (en) 1985-02-22 1989-08-29 Monsanto Company Production of proteins in procaryotes
ES8800957A1 (es) * 1985-02-22 1987-12-01 Monsanto Co Un metodo para la solubilizacion y renaturalizacion de proteina somatotropina
US4652630A (en) * 1985-02-22 1987-03-24 Monsanto Company Method of somatotropin naturation
US6084073A (en) * 1985-03-25 2000-07-04 Chiron Corporation Recombinant ricin toxin
FR2579223B1 (fr) * 1985-03-25 1988-12-09 Genetica Procede de preparation microbiologique de la serum-albumine humaine
DE3511011A1 (de) * 1985-03-27 1986-10-02 Hoechst Ag, 6230 Frankfurt Verfahren zur isolierung und reinigung von (alpha)-interferonen
GB8508340D0 (en) * 1985-03-29 1985-05-09 Creighton T E Production of protein
DE3683186D1 (de) 1985-04-25 1992-02-13 Hoffmann La Roche Rekombinantes humaninterleukin-1.
JPH0640832B2 (ja) * 1985-05-10 1994-06-01 味の素株式会社 インタ−ロイキン２ポリペプチドの回収方法
EP0226639B1 (en) * 1985-05-29 1990-11-14 The Green Cross Corporation Process for preparing heterogenic protein
US5248769A (en) * 1985-06-26 1993-09-28 Cetus Oncology Corporation Process for recovering refractile bodies containing heterologous proteins from microbial hosts
EP0229110B1 (en) 1985-06-26 1992-05-20 The Upjohn Company Solubilization and oxidation of somatotropin from transformed microorganisms
US4748234A (en) * 1985-06-26 1988-05-31 Cetus Corporation Process for recovering refractile bodies containing heterologous proteins from microbial hosts
US4766224A (en) * 1985-08-19 1988-08-23 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
US4656255A (en) * 1985-09-09 1987-04-07 International Minerals & Chemical Corp. Protein recovery
DE3537708A1 (de) * 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
US5453363A (en) * 1985-10-23 1995-09-26 Boehringer Mannheim Gmbh Process for the activation of t-PA or Ing after genetic expression in prokaryotes
US4766205A (en) * 1985-11-13 1988-08-23 Beatrice Companies, Inc. Method for isolation of recombinant polypeptides in biologically active forms
US4734362A (en) * 1986-02-03 1988-03-29 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and products thereof
US5831022A (en) * 1986-02-18 1998-11-03 Hoffmann-La Roche Inc. Purification of recombinant human IL-1α
FR2594846B1 (fr) * 1986-02-21 1989-10-20 Genetica Procede de preparation de la serum albumine humaine mature
US4689401A (en) * 1986-03-06 1987-08-25 Cetus Corporation Method of recovering microbially produced recombinant ricin toxin a chain
DE3611817A1 (de) * 1986-04-08 1987-10-15 Boehringer Mannheim Gmbh Verfahren zur renaturierung von proteinen
US4705848A (en) * 1986-06-02 1987-11-10 International Minerals & Chemical Corp. Isolation of bioactive, monomeric growth hormone
DE3618817A1 (de) * 1986-06-04 1987-12-10 Behringwerke Ag Verfahren zur gewinnung aktiver proteine aus einer biologisch inaktiven form
US4801686A (en) * 1986-09-04 1989-01-31 Immunex Corporation Purification of recombinant interleukin-1
US5179199A (en) * 1986-10-20 1993-01-12 Genzyme Corporation Protein purification
AU612133B2 (en) * 1987-02-20 1991-07-04 Natinco Nv Production of proteins in active forms
US5026828A (en) * 1987-02-27 1991-06-25 Merck & Co., Inc. Method of purifying recombinant pres-1/S-2/S/S hepatitis B antigen from yeast
IL86090A (en) * 1987-04-16 1993-03-15 Cetus Oncology Corp Production of purified, biologically active, bacterially produced recombinant human csf-1
US4929700A (en) * 1987-04-16 1990-05-29 Cetus Corporation Production of purified, biologically active, bacterially produced recombinant human CSF-1
CA1339757C (en) 1987-04-16 1998-03-17 Robert F. Halenbeck Production of purified biologically active, bacterially produced recombinant human csf-1
WO1988008428A1 (en) * 1987-04-28 1988-11-03 Amgen Inc. Method for purifying granulocyte/macrophage colony stimulating factor
US5162507A (en) * 1987-05-11 1992-11-10 Cetus Corporation Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
WO1988008849A1 (en) * 1987-05-11 1988-11-17 Cetus Corporation Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
US4931543A (en) * 1987-05-11 1990-06-05 Cetus Corporation Process for recovering microbially produced interleukin-2
JP2669859B2 (ja) * 1987-08-04 1997-10-29 協和醗酵工業株式会社 タンパク質の再活性化法
US4985544A (en) * 1987-08-04 1991-01-15 Kyowa Hakko Kogyo Co., Ltd. Process for renaturing fish growth hormone
US5011915A (en) * 1987-10-26 1991-04-30 Merck & Co., Inc. Process for purifying recombinant hepatitis antigens
EP0325691B1 (de) * 1988-01-25 1993-04-07 Consortium für elektrochemische Industrie GmbH Verfahren zur Solubilisierung von unlöslichem Protein
JPH01257491A (ja) * 1988-04-08 1989-10-13 Tosoh Corp 不溶性融合異種蛋白質の処理方法
US4999422A (en) * 1988-04-15 1991-03-12 Biogen, N.V. Continuous method of refolding proteins
JP2812760B2 (ja) * 1988-05-31 1998-10-22 カイロン コーポレイション 微生物宿主から異種蛋白質を含有する屈折体を回収する方法
US5089473A (en) 1988-08-29 1992-02-18 Monsanto Company Somatotropin variants and their use
CA1340586C (en) * 1988-09-23 1999-06-08 Cetus Corporation Process for recovering microbially produced interferon-beta
DE3832898A1 (de) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh Praeparat von in prokaryonten exprimiertem plasminogenaktivator
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
US5179196A (en) * 1989-05-04 1993-01-12 Sri International Purification of proteins employing ctap-iii fusions
GB8927546D0 (en) * 1989-12-06 1990-02-07 Ciba Geigy Process for the production of biologically active tgf-beta
DE4039415A1 (de) 1990-02-03 1991-08-08 Boehringer Mannheim Gmbh Verfahren zur herstellung rekombinanter proteine ohne n-terminalen methioninrest
US5023323A (en) * 1990-09-12 1991-06-11 Monsanto Company Method of somatotropin naturation
JPH04113933U (ja) * 1991-03-22 1992-10-06 富泰 本多 化粧板
JPH084290Y2 (ja) * 1991-03-22 1996-02-07 富泰 本多 化粧板
US6869925B1 (en) * 1992-09-09 2005-03-22 Amgen Inc. Inhibition of retrovirus infection
US5399677A (en) * 1993-12-07 1995-03-21 Genetics Institute, Inc. Mutants of bone morphogenetic proteins
US6017880A (en) * 1994-03-09 2000-01-25 Amgen Inc. Inhibition of retrovirus infection
US5437986A (en) * 1994-06-22 1995-08-01 Schering Corporation Extraction of heterologous insoluble proteins from bacteria
FI970230L (fi) * 1994-07-25 1997-01-20 Ciba Geigy Ag Menetelmä proteiinien, kuten yhdistelmä-DNA-hirudiinin tai -epidermaalisen kasvutekijän laskostamiseksi
TW517059B (en) 1994-07-25 2003-01-11 Ciba Geigy Ag New process for the production of biologically active protein
US6037145A (en) * 1994-09-07 2000-03-14 Suntory Limited Process for production of protein
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
EP0904355B1 (de) * 1996-06-11 2004-03-03 Boehringer Mannheim Gmbh Verfahren zur aktivierung von denaturiertem protein
US5914390A (en) * 1997-05-12 1999-06-22 Celtrix Pharmaceuticals, Inc. Methods for increasing yields of recombinant proteins
WO1999010483A2 (de) * 1997-08-22 1999-03-04 Roche Diagnostics Gmbh Autokatalytisch aktivierbare vorstufen von proteasen und deren verwendung
JP3734634B2 (ja) 1999-03-03 2006-01-11 ヒゲタ醤油株式会社 タンパク質の活性化方法及び該装置
IN187900B (en, 2012) * 2000-02-01 2002-07-20 Torrent Pharmaceuticals Ltd
KR100409092B1 (ko) * 2000-12-22 2003-12-11 주)녹십자 단백질의 제조 방법
KR101099398B1 (ko) 2003-09-30 2011-12-27 아스비오파마 가부시키가이샤 ＯｍｐＴ 프로테아제 변이체를 이용한 폴리펩티드의 절단 방법
EP1910415A4 (en) * 2005-07-25 2010-08-11 Trubion Pharmaceuticals Inc COMPOSITIONS AND PROCESSES FOR PROTEIN DEGREGATION
EP2547699B1 (en) 2010-03-17 2016-10-19 ratiopharm GmbH Method for obtaining biologically active recombinant human g-csf
JP5805943B2 (ja) * 2010-12-08 2015-11-10 株式会社北里大塚バイオメディカルアッセイ研究所 百日咳菌のＦｉｍ３の製造方法、及び、百日咳の検出方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4350764A (en) * 1980-03-10 1982-09-21 The Regents Of The University Of California Microbiological synthesis of beta endorphin
AU564690B2 (en) * 1980-04-03 1987-08-20 Biogen Idec Ma Inc. Process for producing human fibroblast interferon-like polypeptides using recombinant dna
IL63916A0 (en) * 1980-09-25 1981-12-31 Genentech Inc Microbial production of human fibroblast interferon
AU561343B2 (en) * 1981-10-19 1987-05-07 Genentech Inc. Human immune interferon by recombinant dna
JPS58157800A (ja) * 1982-03-16 1983-09-19 Sankyo Co Ltd プラスミドｐｓａｎ１８１

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USD1086051S1 (en) * 2022-09-30 2025-07-29 Maxcyte, Inc. Electroporation gasket

Also Published As

Publication number Publication date
IL70474A0 (en) 1984-03-30
NZ206615A (en) 1988-03-30
PT77866B (en) 1986-04-09
MX166885B (es) 1993-02-10
CA1219234A (en) 1987-03-17
EP0114506B1 (en) 1989-11-15
BR8307086A (pt) 1985-02-12
AU2259483A (en) 1984-06-28
GR79124B (en, 2012) 1984-10-02
AU568109B2 (en) 1987-12-17
HU197357B (en) 1989-03-28
EP0114506A1 (en) 1984-08-01
JP2575604B2 (ja) 1997-01-29
JPS59161321A (ja) 1984-09-12
DE3380850D1 (en) 1989-12-21
PT77866A (en) 1984-01-01

Similar Documents

Publication Publication Date Title
DE3380850D1 (en) Methods of purification and reactivation of precipitated heterologous proteins
GB8310425D0 (en) Filtration of suspension
GB2178041B (en) Antigenic modification of peptides
GB2147822B (en) Purification of helium
PT79555A (en) Highly purified protein production and use thereof
GB8332644D0 (en) Affinity purification of antigens
PH19216A (en) Purification of interferon
DE3361838D1 (en) Purification method of human interferon
GB8530016D0 (en) Treatment of particulate material
AU576191B2 (en) Reactivation of recombinant rennet
IL71754A0 (en) Process of purification of dextranesaccharase
ZA836577B (en) Immunomodulator and method of use for immunomodulation
IL80448A0 (en) Purification of proteins
GB2119298B (en) Method of abrading surfaces
ZA839431B (en) Methods of purification and reactivation of precipitated heterologous proteins
GB8529533D0 (en) Treatment of particles
PH24747A (en) Novel aryloxycycloalkanolaminoalkylene aryl ketons and method of use thereof
PH20266A (en) Treatment of endometriosis
ZA841395B (en) Purification of interferon
IL72511A0 (en) Process for the isolation and purification of interferon
GB2119794B (en) Purification of trehalose dimycolates
GB8314853D0 (en) Production and immobillisation of modified proteins
GB8308454D0 (en) Manipulation of particles
GB8332045D0 (en) Orientation of magnetic particles
GB8328990D0 (en) Manipulation of particulate matter

Legal Events

Date Code Title Description
KB Patent renewed
KB Patent renewed
EXP Patent expired