DE68921186T2 - Verfahren zur gewinnung von heterologe proteine enthaltenden refraktilkörpern aus mikrobiellen wirten. - Google Patents

Verfahren zur gewinnung von heterologe proteine enthaltenden refraktilkörpern aus mikrobiellen wirten.

Info

Publication number
DE68921186T2
DE68921186T2 DE68921186T DE68921186T DE68921186T2 DE 68921186 T2 DE68921186 T2 DE 68921186T2 DE 68921186 T DE68921186 T DE 68921186T DE 68921186 T DE68921186 T DE 68921186T DE 68921186 T2 DE68921186 T2 DE 68921186T2
Authority
DE
Germany
Prior art keywords
obtaining
protein
disruption
microbial hosts
refractile bodies
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE68921186T
Other languages
English (en)
Other versions
DE68921186D1 (de
Inventor
Glenn Dorin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Original Assignee
Cetus Oncology Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cetus Oncology Corp filed Critical Cetus Oncology Corp
Application granted granted Critical
Publication of DE68921186D1 publication Critical patent/DE68921186D1/de
Publication of DE68921186T2 publication Critical patent/DE68921186T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
DE68921186T 1988-05-31 1989-05-09 Verfahren zur gewinnung von heterologe proteine enthaltenden refraktilkörpern aus mikrobiellen wirten. Expired - Lifetime DE68921186T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US20074188A 1988-05-31 1988-05-31
PCT/US1989/001986 WO1989012092A1 (en) 1988-05-31 1989-05-09 Process for recovering refractile bodies containing heterologous proteins from microbial hosts

Publications (2)

Publication Number Publication Date
DE68921186D1 DE68921186D1 (de) 1995-03-23
DE68921186T2 true DE68921186T2 (de) 1995-06-14

Family

ID=22742989

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68921186T Expired - Lifetime DE68921186T2 (de) 1988-05-31 1989-05-09 Verfahren zur gewinnung von heterologe proteine enthaltenden refraktilkörpern aus mikrobiellen wirten.

Country Status (7)

Country Link
EP (1) EP0377023B1 (de)
JP (1) JP2812760B2 (de)
AT (1) ATE118544T1 (de)
AU (1) AU3754389A (de)
CA (1) CA1329373C (de)
DE (1) DE68921186T2 (de)
WO (1) WO1989012092A1 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5109119A (en) * 1989-06-06 1992-04-28 Schering Corporation Crystalline r-h-gm-csf and method
GB9107846D0 (en) * 1990-04-30 1991-05-29 Ici Plc Polypeptides
EP0591548A4 (en) * 1992-03-30 1999-04-28 Suntory Ltd Anti-hbs antibody gene and expression plasmid thereof
AU2020329234A1 (en) 2019-08-13 2022-03-24 Repligen Corporation Control systems and methods for automated clarification of cell culture with high solids content

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GR79124B (de) * 1982-12-22 1984-10-02 Genentech Inc
US4748234A (en) * 1985-06-26 1988-05-31 Cetus Corporation Process for recovering refractile bodies containing heterologous proteins from microbial hosts
US4734362A (en) * 1986-02-03 1988-03-29 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and products thereof

Also Published As

Publication number Publication date
JP2812760B2 (ja) 1998-10-22
CA1329373C (en) 1994-05-10
ATE118544T1 (de) 1995-03-15
WO1989012092A1 (en) 1989-12-14
AU3754389A (en) 1990-01-05
EP0377023B1 (de) 1995-02-15
EP0377023A1 (de) 1990-07-11
JPH02504473A (ja) 1990-12-20
DE68921186D1 (de) 1995-03-23

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: CHIRON CORP. (N.D.GES.D. STAATES DELAWARE), EMERYV

8327 Change in the person/name/address of the patent owner

Owner name: NOVARTIS VACCINES AND DIAGNOSTICS,INC.(N.D.GES, US