FI119697B - Subtilisin variantteja - Google Patents
Subtilisin variantteja Download PDFInfo
- Publication number
- FI119697B FI119697B FI961631A FI961631A FI119697B FI 119697 B FI119697 B FI 119697B FI 961631 A FI961631 A FI 961631A FI 961631 A FI961631 A FI 961631A FI 119697 B FI119697 B FI 119697B
- Authority
- FI
- Finland
- Prior art keywords
- subtilisin
- ala
- ser
- gly
- amino acid
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- 239000002243 precursor Substances 0.000 claims abstract description 45
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Classifications
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38609—Protease or amylase in solid compositions only
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Detergent Compositions (AREA)
- Saccharide Compounds (AREA)
- Fats And Perfumes (AREA)
- Peptides Or Proteins (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13724093A | 1993-10-14 | 1993-10-14 | |
| US13724093 | 1993-10-14 | ||
| US9411562 | 1994-10-13 | ||
| PCT/US1994/011562 WO1995010615A1 (en) | 1993-10-14 | 1994-10-13 | Subtilisin variants |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI961631A0 FI961631A0 (fi) | 1996-04-12 |
| FI961631L FI961631L (fi) | 1996-04-15 |
| FI119697B true FI119697B (fi) | 2009-02-13 |
Family
ID=22476438
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI961631A FI119697B (fi) | 1993-10-14 | 1996-04-12 | Subtilisin variantteja |
Country Status (24)
| Country | Link |
|---|---|
| US (2) | US6586221B2 (pl) |
| EP (2) | EP0723590B1 (pl) |
| JP (1) | JP4086207B2 (pl) |
| KR (1) | KR100344976B1 (pl) |
| CN (1) | CN1056649C (pl) |
| AT (1) | ATE284962T1 (pl) |
| AU (1) | AU700373B2 (pl) |
| BR (1) | BR9407825A (pl) |
| CA (1) | CA2173973C (pl) |
| CZ (1) | CZ289323B6 (pl) |
| DE (1) | DE69434187T2 (pl) |
| DK (1) | DK0723590T3 (pl) |
| ES (1) | ES2235164T3 (pl) |
| FI (1) | FI119697B (pl) |
| HK (1) | HK1000649A1 (pl) |
| MA (1) | MA23346A1 (pl) |
| NO (1) | NO325568B1 (pl) |
| NZ (1) | NZ274998A (pl) |
| PL (1) | PL178478B1 (pl) |
| PT (1) | PT723590E (pl) |
| RU (1) | RU2136756C1 (pl) |
| TW (1) | TW448230B (pl) |
| WO (1) | WO1995010615A1 (pl) |
| ZA (1) | ZA948086B (pl) |
Families Citing this family (89)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2287931T3 (es) * | 1993-10-14 | 2007-12-16 | THE PROCTER & GAMBLE COMPANY | Composiciones limpiadoras que contienen proteasa. |
| MA23346A1 (fr) * | 1993-10-14 | 1995-04-01 | Genencor Int | Variantes de la subtilisine |
| AU1253695A (en) * | 1993-10-14 | 1995-05-04 | Procter & Gamble Company, The | Bleaching compositions comprising protease enzymes |
| EP0687733A1 (en) * | 1994-06-16 | 1995-12-20 | The Procter & Gamble Company | Detergent composition containing wool compatible high alkaline proteases |
| US6066611A (en) * | 1994-10-13 | 2000-05-23 | The Procter & Gamble Company | Bleaching compositions comprising protease enzymes |
| US5837517A (en) * | 1995-05-05 | 1998-11-17 | Novo Nordisk A/S | Protease variants and compositions |
| EP0827531A2 (en) * | 1995-05-05 | 1998-03-11 | Unilever N.V. | Subtilisin variants |
| DE19530816A1 (de) * | 1995-08-23 | 1997-02-27 | Cognis Bio Umwelt | Verwendung von mutierter Subtilisin-Protease in kosmetischen Produkten |
| DE19535082A1 (de) | 1995-09-21 | 1997-03-27 | Henkel Ecolab Gmbh & Co Ohg | Pastenförmiges Wasch- und Reinigungsmittel |
| US5858948A (en) * | 1996-05-03 | 1999-01-12 | Procter & Gamble Company | Liquid laundry detergent compositions comprising cotton soil release polymers and protease enzymes |
| DE19636035A1 (de) | 1996-09-05 | 1998-03-12 | Henkel Ecolab Gmbh & Co Ohg | Pastenförmiges Wasch- und Reinigungsmittel |
| BR9712878A (pt) * | 1996-11-04 | 2000-02-01 | Novo Nordisk As | Variante de enzima subtilase, processos para a identificação de uma variante de protease apresentando estabilidade autoproteolìtica e paraq a produção de uma enzima subtilase mutante e de uma variante de subtilase, sequência de dna, vetor, célula hospedeira microbiana, composição e uso de uma variante de subtilase. |
| DE19703364A1 (de) | 1997-01-30 | 1998-08-06 | Henkel Ecolab Gmbh & Co Ohg | Pastenförmiges Wasch- und Reinigungsmittel |
| ES2210751T3 (es) * | 1997-06-04 | 2004-07-01 | THE PROCTER & GAMBLE COMPANY | Particulas de enzimas detergentes que tienen una capa barrera de carboxilato soluble en agua y composiciones que incluyen las mismas. |
| CA2291646A1 (en) * | 1997-06-04 | 1998-12-10 | Saroj Rai | Mixed protease enzyme systems for cleaning protein based soils and compositions incorporating same |
| DK0985042T3 (da) * | 1997-06-04 | 2007-01-15 | Genencor Int | Proteaseenzymer til vanskelig rengöring og/eller plet- og filmreduktion samt præparater, der inkorporerer disse |
| US6284246B1 (en) | 1997-07-30 | 2001-09-04 | The Procter & Gamble Co. | Modified polypeptides with high activity and reduced allergenicity |
| AU2002325461B2 (en) * | 1997-10-23 | 2006-05-11 | Genencor International, Inc. | Multiply-substituted protease variants with altered net charge for use in detergents |
| AR015977A1 (es) | 1997-10-23 | 2001-05-30 | Genencor Int | Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes |
| ES2368718T3 (es) * | 1997-10-23 | 2011-11-21 | Danisco Us Inc. | Variantes de subtilisina con múltiples sustituciones. |
| AU2207099A (en) * | 1997-12-24 | 1999-07-19 | Genencor International, Inc. | An improved method of assaying for a preferred enzyme and/or preferred detergentcomposition |
| US6599731B1 (en) | 1997-12-30 | 2003-07-29 | Genencor International, Inc. | Proteases from gram positive organisms |
| US6465186B1 (en) | 1997-12-30 | 2002-10-15 | Genecor International, Inc. | Proteases from gram positive organisms |
| GB9727470D0 (en) | 1997-12-30 | 1998-02-25 | Genencor Int Bv | Proteases from gram positive organisms |
| US6528255B1 (en) | 1997-12-30 | 2003-03-04 | Genencor International, Inc. | Proteases from gram positive organisms |
| CN1301305A (zh) | 1998-03-26 | 2001-06-27 | 宝洁公司 | 具有氨基酸取代的丝氨酸蛋白酶变体 |
| US6908757B1 (en) | 1998-03-26 | 2005-06-21 | The Procter & Gamble Company | Serine protease variants having amino acid deletions and substitutions |
| US6495136B1 (en) | 1998-03-26 | 2002-12-17 | The Procter & Gamble Company | Proteases having modified amino acid sequences conjugated to addition moieties |
| DE19857687A1 (de) | 1998-12-15 | 2000-06-21 | Henkel Ecolab Gmbh & Co Ohg | Pastenförmiges Waschmittel |
| CA2355580C (en) * | 1998-12-18 | 2011-03-15 | Novozymes A/S | Subtilase enzymes of the i-s1 and i-s2 sub-groups having an additional amino acid residue in the active site loop region |
| EP1141335B1 (en) | 1998-12-21 | 2009-08-05 | Genencor International, Inc. | Chemically modified enzymes with multiple charged variants |
| US6946128B1 (en) | 1999-07-22 | 2005-09-20 | The Procter & Gamble Company | Protease conjugates having sterically protected epitope regions |
| JP2003505067A (ja) | 1999-07-22 | 2003-02-12 | ザ、プロクター、エンド、ギャンブル、カンパニー | 特定のエピトープ領域にアミノ酸欠失及び置換を有するサブチリシンプロテアーゼの変異体 |
| MXPA02000840A (es) | 1999-07-22 | 2002-07-30 | Procter & Gamble | Variantes de proteasa de subtilisina que tienen substituciones de aminoacidos en regiones de epitopes definidas. |
| EP1196548A2 (en) | 1999-07-22 | 2002-04-17 | The Procter & Gamble Company | Protease conjugates having sterically protected clip sites |
| AU5911599A (en) * | 1999-09-09 | 2001-04-10 | Procter & Gamble Company, The | A detergent composition containing a protease |
| AU782372B2 (en) * | 1999-12-15 | 2005-07-21 | Novozymes A/S | Subtilase variants having an improved wash performance on egg stains |
| JP2003516751A (ja) * | 1999-12-15 | 2003-05-20 | ノボザイムス アクティーゼルスカブ | 卵の染みに及ぼす洗浄性能改良を示すズブチラーゼ変異体 |
| US6777218B1 (en) * | 2000-03-14 | 2004-08-17 | Novozymes A/S | Subtilase enzymes having an improved wash performance on egg stains |
| US7303907B2 (en) | 2001-01-08 | 2007-12-04 | Health Protection Agency | Degradation and detection of TSE infectivity |
| WO2002077187A2 (en) * | 2001-03-23 | 2002-10-03 | Genencor International, Inc. | Proteins producing an altered immunogenic response and methods of making and using the same |
| DK200101090A (da) | 2001-07-12 | 2001-08-16 | Novozymes As | Subtilase variants |
| US7153820B2 (en) | 2001-08-13 | 2006-12-26 | Ecolab Inc. | Solid detergent composition and method for solidifying a detergent composition |
| US20030157645A1 (en) * | 2001-12-21 | 2003-08-21 | Direvo Bio Tech Ag. | Subtilisin variants with improved characteristics |
| JP2005535284A (ja) * | 2001-12-31 | 2005-11-24 | ジェネンコー・インターナショナル・インク | 免疫反応の変化を生じるプロテアーゼ、ならびにその製造および利用方法 |
| DK1523553T3 (da) * | 2002-01-16 | 2010-04-19 | Genencor Int | Multi-substituerede proteasevarianter |
| AU2003210551A1 (en) * | 2002-01-16 | 2003-09-02 | Genencor International, Inc. | Multiply-substituted protease variants |
| BRPI0409992A (pt) * | 2003-05-07 | 2006-05-09 | Novozymes As | enzima subtilase, polinucleotìdeo isolado, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, método para produzir a subtilase, composição limpadora ou detergente, usos de uma subtilase e de uma composição limpadora ou detergente, e, métodos para limpar ou lavar louças, lavar uma superfìcie dura ou lavar roupas e para remover manchas de ovo de uma superfìcie dura ou de roupas para lavar |
| US20090060933A1 (en) * | 2004-06-14 | 2009-03-05 | Estell David A | Proteases producing an altered immunogenic response and methods of making and using the same |
| PL2586456T3 (pl) | 2004-10-29 | 2016-07-29 | Ratiopharm Gmbh | Remodeling i glikopegilacja czynnika wzrostu fibroblastów (FGF) |
| KR20150115023A (ko) * | 2006-07-18 | 2015-10-13 | 다니스코 유에스 인크. | 광범위한 온도에 걸쳐 활성인 프로테아제 변이형 |
| MX2009003637A (es) * | 2006-10-16 | 2009-04-22 | Danisco Us Inc Genencor Div | Detergentes sin fosfato para lavar platos. |
| US8093200B2 (en) | 2007-02-15 | 2012-01-10 | Ecolab Usa Inc. | Fast dissolving solid detergent |
| KR101575741B1 (ko) | 2007-10-18 | 2015-12-08 | 에코랍 인코퍼레이티드 | 압축된 왁시 고형 세정 조성물 및 이의 제조 방법 |
| EP2297314B1 (en) * | 2008-06-06 | 2015-04-15 | Danisco US Inc. | Compositions and methods comprising variant microbial proteases |
| EP3031894A1 (en) * | 2008-11-11 | 2016-06-15 | Danisco US Inc. | Proteases comprising one or more combinable mutations |
| BRPI0920925A2 (pt) | 2008-11-11 | 2015-08-18 | Danisco Us Inc | Composições e métodos compreendendo uma variante de subtilisina |
| CN102421893B (zh) * | 2009-04-30 | 2014-10-29 | 花王株式会社 | 碱性蛋白酶变体 |
| DE102009029513A1 (de) * | 2009-09-16 | 2011-03-24 | Henkel Ag & Co. Kgaa | Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Proteasen |
| BR112012028467B1 (pt) * | 2010-05-06 | 2021-02-17 | Danisco Us Inc. | variantes de subtilisina, seu método de produção, ácido nucleico, e método de limpeza |
| DE102010028951A1 (de) | 2010-05-12 | 2011-11-17 | Henkel Ag & Co. Kgaa | Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Protease und Lipase |
| DE102010063458A1 (de) * | 2010-12-17 | 2012-06-21 | Henkel Ag & Co. Kgaa | Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Protease und Amylase |
| DE102010063457A1 (de) * | 2010-12-17 | 2012-06-21 | Henkel Ag & Co. Kgaa | Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Protease und Cellulase |
| CN103764823B (zh) * | 2011-05-05 | 2018-05-11 | 丹尼斯科美国公司 | 包含丝氨酸蛋白酶变体的组合物和方法 |
| BR112013028007A2 (pt) | 2011-05-20 | 2017-01-10 | Ecolab Usa Inc | detergentes não-fosfato e ácidos não-fosfóricos em um sistema alternado alcalinidade/acidez para a lavagem de louça |
| CA2856820C (en) | 2011-05-20 | 2019-10-29 | Ecolab Usa Inc. | Acid formulations for use in a system for warewashing |
| CN103998590B (zh) | 2011-12-13 | 2019-02-01 | 艺康美国股份有限公司 | 浓的器皿洗涤组合物和方法 |
| DE102012201522A1 (de) * | 2012-02-02 | 2013-08-08 | Basf Se | Lagerstabiles flüssiges Geschirrspülmittel enthaltend Protease und Amylase |
| EP2628785B1 (en) * | 2012-02-17 | 2016-05-18 | Henkel AG & Co. KGaA | Detergent compositions comprising subtilase variants |
| DE102012215107A1 (de) | 2012-08-24 | 2014-02-27 | Basf Se | Festes Geschirrspülmittel mit verbesserter Proteaseleistung |
| US9745543B2 (en) | 2012-09-10 | 2017-08-29 | Ecolab Usa Inc. | Stable liquid manual dishwashing compositions containing enzymes |
| CN103013960A (zh) * | 2012-12-21 | 2013-04-03 | 青岛蔚蓝生物集团有限公司 | 一种碱性蛋白酶及其重组表达工程菌 |
| CN105518118B (zh) | 2013-09-09 | 2019-09-17 | 艺康美国股份有限公司 | 通过螯合剂组合来协同去污 |
| US9796950B2 (en) | 2013-09-09 | 2017-10-24 | Ecolab Usa Inc. | Synergistic stain removal through an alkali metal hydroxide-based detergent composition with novel chelator combination |
| JP6254693B2 (ja) | 2013-10-24 | 2017-12-27 | エコラボ ユーエスエー インコーポレイティド | 表面から汚れを除去する組成物及び方法 |
| WO2016001450A2 (en) | 2014-07-04 | 2016-01-07 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
| CN107075493B (zh) * | 2014-12-04 | 2020-09-01 | 诺维信公司 | 枯草杆菌酶变体以及编码它们的多核苷酸 |
| RU2733987C2 (ru) * | 2015-05-13 | 2020-10-09 | ДАНИСКО ЮЭс ИНК. | Варианты протеазы клады aprl и их применения |
| CN108291179B (zh) | 2015-11-25 | 2020-07-07 | 荷兰联合利华有限公司 | 液体洗涤剂组合物 |
| EP3464599A1 (en) * | 2016-05-31 | 2019-04-10 | Danisco US Inc. | Protease variants and uses thereof |
| US20190185836A1 (en) * | 2016-09-02 | 2019-06-20 | The Regents Of The University Of California | Engineered subtiligase variants for versatile, site-specific labeling of proteins |
| EP3717643A1 (en) * | 2017-11-29 | 2020-10-07 | Danisco US Inc. | Subtilisin variants having improved stability |
| CN114207101B (zh) | 2019-07-31 | 2024-05-31 | 埃科莱布美国股份有限公司 | 免用个人防护设备的脱灰剂组合物 |
| US11492571B2 (en) * | 2019-10-24 | 2022-11-08 | The Procter & Gamble Company | Automatic dishwashing detergent composition comprising a protease |
| US20230263866A1 (en) * | 2020-05-15 | 2023-08-24 | Jnc Corporation | Antiviral agent |
| WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
| CN114836408B (zh) * | 2022-05-28 | 2023-09-19 | 湖北大学 | 含有前肽突变体的碱性蛋白酶及应用 |
| EP4446411A1 (en) * | 2023-04-14 | 2024-10-16 | Peaccel | Variant subtilisin proteases |
| WO2026030345A1 (en) | 2024-07-29 | 2026-02-05 | Danisco Us Inc. | Signal and pro-region sequence variants for enhanced protease production in bacillus cells |
Family Cites Families (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4760025A (en) * | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
| US5763257A (en) * | 1984-05-29 | 1998-06-09 | Genencor International, Inc. | Modified subtilisins having amino acid alterations |
| US5182204A (en) | 1984-05-29 | 1993-01-26 | Genencor International, Inc. | Non-human carbonyl hydrolase mutants, vectors encoding same and hosts transformed with said vectors |
| US5204015A (en) | 1984-05-29 | 1993-04-20 | Genencor International, Inc. | Subtilisin mutants |
| US5972682A (en) * | 1984-05-29 | 1999-10-26 | Genencor International, Inc. | Enzymatically active modified subtilisins |
| US5155033A (en) | 1989-01-06 | 1992-10-13 | Genencor, Inc. | Subtilisins modified at position 225 resulting in a shift in catalytic activity |
| US5185258A (en) | 1984-05-29 | 1993-02-09 | Genencor International, Inc. | Subtilisin mutants |
| US4990452A (en) | 1986-02-12 | 1991-02-05 | Genex Corporation | Combining mutations for stabilization of subtilisin |
| US5013657A (en) | 1988-04-12 | 1991-05-07 | Bryan Philip N | Subtilisin mutations |
| SG30639G (en) * | 1986-04-30 | 1995-09-01 | Genencor Int | Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors |
| EP0353250B1 (en) | 1987-04-06 | 1999-09-08 | Novo Nordisk A/S | The engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins |
| US4914031A (en) | 1987-04-10 | 1990-04-03 | Amgen, Inc. | Subtilisin analogs |
| DK6488D0 (da) | 1988-01-07 | 1988-01-07 | Novo Industri As | Enzymer |
| WO1989006276A2 (en) | 1988-01-08 | 1989-07-13 | Dpz Deutsches Primatenzentrum Gesellschaft Mbh | Hiv-2-type retroviruses of primates, vaccines, diagnostic and pharmaceutical compositions |
| PT89702B (pt) | 1988-02-11 | 1994-04-29 | Gist Brocades Nv | Processo para a preparacao de novos enzimas proteoliticos e de detergentes que os contem |
| US5324653A (en) | 1988-02-11 | 1994-06-28 | Gist-Brocades N.V. | Recombinant genetic means for the production of serine protease muteins |
| US5116741A (en) * | 1988-04-12 | 1992-05-26 | Genex Corporation | Biosynthetic uses of thermostable proteases |
| SE8801426D0 (sv) * | 1988-04-15 | 1988-04-15 | Ulf Rothman | Forfarande och medel for blodbehandling |
| WO1990014420A1 (en) * | 1989-05-17 | 1990-11-29 | Amgen Inc. | Multiply mutated subtilisins |
| BR9006827A (pt) * | 1989-06-26 | 1991-08-06 | Unilever Nv | Composicoes detergentes enzimaticas |
| DK316989D0 (da) | 1989-06-26 | 1989-06-26 | Novo Nordisk As | Enzymer |
| JPH04500384A (ja) * | 1989-06-26 | 1992-01-23 | ユニリーバー・ナームローゼ・ベンノートシヤープ | 酵素洗剤組成物 |
| US5665587A (en) * | 1989-06-26 | 1997-09-09 | Novo Nordisk A/S | Modified subtilisins and detergent compositions containing same |
| JP2567282B2 (ja) * | 1989-10-02 | 1996-12-25 | 日本ゼオン株式会社 | ポジ型レジスト組成物 |
| DK271490D0 (da) * | 1990-11-14 | 1990-11-14 | Novo Nordisk As | Detergentkomposition |
| FI932832A7 (fi) | 1990-12-21 | 1993-08-13 | Novozymes As | Entsyymimutantteja, joilla on alhainen molekyylivarauksen vaihteluaste pH-alueella |
| US5340735A (en) | 1991-05-29 | 1994-08-23 | Cognis, Inc. | Bacillus lentus alkaline protease variants with increased stability |
| US5316935A (en) * | 1992-04-06 | 1994-05-31 | California Institute Of Technology | Subtilisin variants suitable for hydrolysis and synthesis in organic media |
| JP4028592B2 (ja) | 1992-07-17 | 2007-12-26 | ジェネンコー インターナショナル インコーポレイテッド | 高アルカリ性セリンプロテアーゼ |
| DK39093D0 (da) | 1993-04-01 | 1993-04-01 | Novo Nordisk As | Enzym |
| ES2287931T3 (es) * | 1993-10-14 | 2007-12-16 | THE PROCTER & GAMBLE COMPANY | Composiciones limpiadoras que contienen proteasa. |
| MA23346A1 (fr) * | 1993-10-14 | 1995-04-01 | Genencor Int | Variantes de la subtilisine |
| AU1253695A (en) * | 1993-10-14 | 1995-05-04 | Procter & Gamble Company, The | Bleaching compositions comprising protease enzymes |
| AR015977A1 (es) * | 1997-10-23 | 2001-05-30 | Genencor Int | Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes |
| US6835550B1 (en) * | 1998-04-15 | 2004-12-28 | Genencor International, Inc. | Mutant proteins having lower allergenic response in humans and methods for constructing, identifying and producing such proteins |
| EP1141335B1 (en) * | 1998-12-21 | 2009-08-05 | Genencor International, Inc. | Chemically modified enzymes with multiple charged variants |
| WO2002077187A2 (en) * | 2001-03-23 | 2002-10-03 | Genencor International, Inc. | Proteins producing an altered immunogenic response and methods of making and using the same |
-
1994
- 1994-10-11 MA MA23668A patent/MA23346A1/fr unknown
- 1994-10-13 US US08/322,678 patent/US6586221B2/en not_active Expired - Lifetime
- 1994-10-13 DK DK94931345T patent/DK0723590T3/da active
- 1994-10-13 CZ CZ19961065A patent/CZ289323B6/cs not_active IP Right Cessation
- 1994-10-13 BR BR9407825A patent/BR9407825A/pt not_active IP Right Cessation
- 1994-10-13 JP JP51201395A patent/JP4086207B2/ja not_active Expired - Lifetime
- 1994-10-13 EP EP94931345A patent/EP0723590B1/en not_active Expired - Lifetime
- 1994-10-13 KR KR1019960701923A patent/KR100344976B1/ko not_active Expired - Lifetime
- 1994-10-13 PL PL94313942A patent/PL178478B1/pl unknown
- 1994-10-13 ES ES94931345T patent/ES2235164T3/es not_active Expired - Lifetime
- 1994-10-13 HK HK97102150A patent/HK1000649A1/en not_active IP Right Cessation
- 1994-10-13 AU AU80157/94A patent/AU700373B2/en not_active Expired
- 1994-10-13 WO PCT/US1994/011562 patent/WO1995010615A1/en not_active Ceased
- 1994-10-13 CN CN94193785A patent/CN1056649C/zh not_active Expired - Lifetime
- 1994-10-13 PT PT94931345T patent/PT723590E/pt unknown
- 1994-10-13 RU RU96109372A patent/RU2136756C1/ru active
- 1994-10-13 NZ NZ274998A patent/NZ274998A/en not_active IP Right Cessation
- 1994-10-13 AT AT94931345T patent/ATE284962T1/de active
- 1994-10-13 EP EP04024046A patent/EP1526182A3/en not_active Withdrawn
- 1994-10-13 DE DE69434187T patent/DE69434187T2/de not_active Expired - Lifetime
- 1994-10-13 CA CA002173973A patent/CA2173973C/en not_active Expired - Lifetime
- 1994-10-14 ZA ZA948086A patent/ZA948086B/xx unknown
-
1995
- 1995-01-18 TW TW084100420A patent/TW448230B/zh not_active IP Right Cessation
-
1996
- 1996-04-12 FI FI961631A patent/FI119697B/fi not_active IP Right Cessation
- 1996-04-12 NO NO19961468A patent/NO325568B1/no not_active IP Right Cessation
-
2002
- 2002-12-18 US US10/323,324 patent/US7250281B2/en not_active Expired - Fee Related
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| EP1183338B1 (en) | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 97 and 98 | |
| EP1141260B1 (en) | Subtilase enzymes of the i-s1 and i-s2 sub-groups having an additional amino acid residue in an active site loop region | |
| EP1141258B1 (en) | Subtilase enzymes of the i-s1 and i-s2 sub-groups having an additional amino acid residue in an active site loop region | |
| EP1183342B1 (en) | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 128 and 129 | |
| EP1183341B1 (en) | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 127 and 128 | |
| EP1141259B1 (en) | Subtilase enzymes of the i-s1 and i-s2 sub-groups having an additional amino acid residue in an active site loop region | |
| EP1803817B1 (en) | Subtilase enzymes of the I-S1 and I-S2 sub-groups having an additional amino acid residue in an active site loop region | |
| HK1071398A (en) | Subtilisin variants | |
| KR20010093167A (ko) | 활성부위 루프 영역에 추가적 아미노산 잔기를 가지는서브그룹 i-s1과 i-s2의 서브틸라제 효소 | |
| KR20010093172A (ko) | 활성부위 루프 영역에 추가적 아미노산 잔기를 가지는서브그룹 i-s1과 i-s2의 서브틸라제 효소 | |
| EP1183339A1 (en) | Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 129 and 130 |
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