CA2173973A1 - Subtilisin variants - Google Patents

Subtilisin variants

Info

Publication number
CA2173973A1
CA2173973A1 CA002173973A CA2173973A CA2173973A1 CA 2173973 A1 CA2173973 A1 CA 2173973A1 CA 002173973 A CA002173973 A CA 002173973A CA 2173973 A CA2173973 A CA 2173973A CA 2173973 A1 CA2173973 A1 CA 2173973A1
Authority
CA
Canada
Prior art keywords
carbonyl
hydrolase
amino acid
precursor
hydrolases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002173973A
Other languages
French (fr)
Other versions
CA2173973C (en
Inventor
Thomas P. Graycar
Richard R. Bott
Lori J. Wilson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Thomas P. Graycar
Richard R. Bott
Lori J. Wilson
Genencor International, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Thomas P. Graycar, Richard R. Bott, Lori J. Wilson, Genencor International, Inc. filed Critical Thomas P. Graycar
Publication of CA2173973A1 publication Critical patent/CA2173973A1/en
Application granted granted Critical
Publication of CA2173973C publication Critical patent/CA2173973C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38609—Protease or amylase in solid compositions only
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Abstract

Novel carbonyl hydrolase variants derived from the DNA sequences of naturally-occuring or recombinant non-human carbonyl hydrolases are disclosed. The variant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such variant carbonyl hydrolases have properties which are different from those of the precursor hydrolase, such as altered proteolytic activity, altered stability, etc. The substituted amino acid residues correspond to positions +76 in combination with one or more of the folloqing residues +99, +101, +103, +104, +107, +123, +27, +105, +109, +126, +128, +135, +156, +166, +195, +197, +204, +206, +210, +216, +217, +218, +222, +260, +265 and/or +274 in Bacillus amyloliquefaciens subtilisin.
CA002173973A 1993-10-14 1994-10-13 Subtilisin variants Expired - Lifetime CA2173973C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13724093A 1993-10-14 1993-10-14
US08/137,240 1993-10-14
PCT/US1994/011562 WO1995010615A1 (en) 1993-10-14 1994-10-13 Subtilisin variants

Publications (2)

Publication Number Publication Date
CA2173973A1 true CA2173973A1 (en) 1995-04-20
CA2173973C CA2173973C (en) 2009-10-06

Family

ID=22476438

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002173973A Expired - Lifetime CA2173973C (en) 1993-10-14 1994-10-13 Subtilisin variants

Country Status (24)

Country Link
US (2) US6586221B2 (en)
EP (2) EP1526182A3 (en)
JP (1) JP4086207B2 (en)
KR (1) KR100344976B1 (en)
CN (1) CN1056649C (en)
AT (1) ATE284962T1 (en)
AU (1) AU700373B2 (en)
BR (1) BR9407825A (en)
CA (1) CA2173973C (en)
CZ (1) CZ289323B6 (en)
DE (1) DE69434187T2 (en)
DK (1) DK0723590T3 (en)
ES (1) ES2235164T3 (en)
FI (1) FI119697B (en)
HK (1) HK1000649A1 (en)
MA (1) MA23346A1 (en)
NO (1) NO325568B1 (en)
NZ (1) NZ274998A (en)
PL (1) PL178478B1 (en)
PT (1) PT723590E (en)
RU (1) RU2136756C1 (en)
TW (1) TW448230B (en)
WO (1) WO1995010615A1 (en)
ZA (1) ZA948086B (en)

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DE19636035A1 (en) 1996-09-05 1998-03-12 Henkel Ecolab Gmbh & Co Ohg Paste-like detergent and cleaning agent
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DE19703364A1 (en) 1997-01-30 1998-08-06 Henkel Ecolab Gmbh & Co Ohg Paste-like detergent and cleaning agent
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ES2273417T3 (en) * 1997-06-04 2007-05-01 Genencor International, Inc. ENZYMES PROTEASES TO ELIMINATE DIFFICULT DIRT AND / OR REDUCE THE FORMATION OF SPOTS AND FILMS AND COMPOSCITIONS THAT INCLUDE THEM.
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Also Published As

Publication number Publication date
BR9407825A (en) 1997-05-06
CN1056649C (en) 2000-09-20
PL178478B1 (en) 2000-05-31
KR100344976B1 (en) 2002-11-23
FI961631A (en) 1996-04-15
AU8015794A (en) 1995-05-04
DK0723590T3 (en) 2005-04-25
HK1000649A1 (en) 2005-06-03
EP0723590A1 (en) 1996-07-31
NO961468D0 (en) 1996-04-12
PT723590E (en) 2005-04-29
FI961631A0 (en) 1996-04-12
DE69434187T2 (en) 2005-12-15
EP1526182A2 (en) 2005-04-27
EP1526182A3 (en) 2005-05-04
US20040023353A1 (en) 2004-02-05
FI119697B (en) 2009-02-13
US6586221B2 (en) 2003-07-01
EP0723590B1 (en) 2004-12-15
NO961468L (en) 1996-04-12
RU2136756C1 (en) 1999-09-10
WO1995010615A1 (en) 1995-04-20
NZ274998A (en) 1997-04-24
CA2173973C (en) 2009-10-06
CZ106596A3 (en) 1996-09-11
DE69434187D1 (en) 2005-01-20
CZ289323B6 (en) 2002-01-16
JPH09504170A (en) 1997-04-28
MA23346A1 (en) 1995-04-01
AU700373B2 (en) 1999-01-07
US7250281B2 (en) 2007-07-31
PL313942A1 (en) 1996-08-05
ES2235164T3 (en) 2005-07-01
JP4086207B2 (en) 2008-05-14
CN1133068A (en) 1996-10-09
NO325568B1 (en) 2008-06-23
ATE284962T1 (en) 2005-01-15
TW448230B (en) 2001-08-01
US20030077807A1 (en) 2003-04-24
ZA948086B (en) 1995-06-07

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