DE69434187D1 - Subtilisin varianten - Google Patents

Subtilisin varianten

Info

Publication number
DE69434187D1
DE69434187D1 DE69434187T DE69434187T DE69434187D1 DE 69434187 D1 DE69434187 D1 DE 69434187D1 DE 69434187 T DE69434187 T DE 69434187T DE 69434187 T DE69434187 T DE 69434187T DE 69434187 D1 DE69434187 D1 DE 69434187D1
Authority
DE
Germany
Prior art keywords
carbonyl
hydrolase
amino acid
precursor
hydrolases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69434187T
Other languages
English (en)
Other versions
DE69434187T2 (de
Inventor
P Graycar
R Bott
J Wilson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc filed Critical Genencor International Inc
Application granted granted Critical
Publication of DE69434187D1 publication Critical patent/DE69434187D1/de
Publication of DE69434187T2 publication Critical patent/DE69434187T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38609—Protease or amylase in solid compositions only
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Separation Of Suspended Particles By Flocculating Agents (AREA)
  • Saccharide Compounds (AREA)
  • Fats And Perfumes (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE69434187T 1993-10-14 1994-10-13 Subtilisin varianten Expired - Lifetime DE69434187T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13724093A 1993-10-14 1993-10-14
US137240 1993-10-14
PCT/US1994/011562 WO1995010615A1 (en) 1993-10-14 1994-10-13 Subtilisin variants

Publications (2)

Publication Number Publication Date
DE69434187D1 true DE69434187D1 (de) 2005-01-20
DE69434187T2 DE69434187T2 (de) 2005-12-15

Family

ID=22476438

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69434187T Expired - Lifetime DE69434187T2 (de) 1993-10-14 1994-10-13 Subtilisin varianten

Country Status (24)

Country Link
US (2) US6586221B2 (de)
EP (2) EP1526182A3 (de)
JP (1) JP4086207B2 (de)
KR (1) KR100344976B1 (de)
CN (1) CN1056649C (de)
AT (1) ATE284962T1 (de)
AU (1) AU700373B2 (de)
BR (1) BR9407825A (de)
CA (1) CA2173973C (de)
CZ (1) CZ289323B6 (de)
DE (1) DE69434187T2 (de)
DK (1) DK0723590T3 (de)
ES (1) ES2235164T3 (de)
FI (1) FI119697B (de)
HK (1) HK1000649A1 (de)
MA (1) MA23346A1 (de)
NO (1) NO325568B1 (de)
NZ (1) NZ274998A (de)
PL (1) PL178478B1 (de)
PT (1) PT723590E (de)
RU (1) RU2136756C1 (de)
TW (1) TW448230B (de)
WO (1) WO1995010615A1 (de)
ZA (1) ZA948086B (de)

Families Citing this family (85)

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CA2173105C (en) * 1993-10-14 2003-05-27 Andre Baeck Protease-containing cleaning compositions
BR9407833A (pt) * 1993-10-14 1997-05-13 Procter & Gamble Composições alvejantes compreendendo enzimas protease
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WO1996034935A2 (en) * 1995-05-05 1996-11-07 Unilever N.V. Subtilisin variants
US5837517A (en) * 1995-05-05 1998-11-17 Novo Nordisk A/S Protease variants and compositions
DE19530816A1 (de) * 1995-08-23 1997-02-27 Cognis Bio Umwelt Verwendung von mutierter Subtilisin-Protease in kosmetischen Produkten
DE19535082A1 (de) 1995-09-21 1997-03-27 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Wasch- und Reinigungsmittel
US5858948A (en) * 1996-05-03 1999-01-12 Procter & Gamble Company Liquid laundry detergent compositions comprising cotton soil release polymers and protease enzymes
DE19636035A1 (de) 1996-09-05 1998-03-12 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Wasch- und Reinigungsmittel
ATE409743T1 (de) * 1996-11-04 2008-10-15 Novozymes As Subtilase varianten und verbindungen
DE19703364A1 (de) 1997-01-30 1998-08-06 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Wasch- und Reinigungsmittel
JP2002502460A (ja) * 1997-06-04 2002-01-22 ザ、プロクター、エンド、ギャンブル、カンパニー タンパク質主体の汚れをクリーニングするための混合プロテアーゼ酵素系及びそれを含む組成物
ATE338823T1 (de) * 1997-06-04 2006-09-15 Genecor Int Inc Protease zur gründlichen reinigung und/oder flecken- und filmreduktion und zusammensetzungen damit
DK0988366T3 (da) * 1997-06-04 2004-03-29 Procter & Gamble Rensende enzympartikler, der har et vandopløseligt carboxylatbarrierelag, og sammensætninger, der indbefatter samme
US6284246B1 (en) 1997-07-30 2001-09-04 The Procter & Gamble Co. Modified polypeptides with high activity and reduced allergenicity
AR015977A1 (es) * 1997-10-23 2001-05-30 Genencor Int Variantes de proteasa multiplemente substituida con carga neta alterada para su empleo en detergentes
AU2002325461B2 (en) * 1997-10-23 2006-05-11 Genencor International, Inc. Multiply-substituted protease variants with altered net charge for use in detergents
ES2367505T3 (es) * 1997-10-23 2011-11-04 Danisco Us Inc. Variantes de proteasa con sustituciones múltiples con carga neta alterada para usar en detergentes.
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DE69941223D1 (de) 1998-12-21 2009-09-17 Billig Fritz Chemisch modifizierte enzymen mit mehrfachgeladenen varianten
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WO2001044452A1 (en) * 1999-12-15 2001-06-21 Novozymes A/S Subtilase variants having an improved wash performance on egg stains
JP2003516751A (ja) * 1999-12-15 2003-05-20 ノボザイムス アクティーゼルスカブ 卵の染みに及ぼす洗浄性能改良を示すズブチラーゼ変異体
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AU2007275864B2 (en) * 2006-07-18 2013-05-02 Danisco Us, Inc., Genencor Division Protease variants active over a broad temperature range
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US20110256609A1 (en) * 2008-06-06 2011-10-20 Basler Joshua R Compositions And Methods Comprising Variant Microbial Proteases
WO2010056634A1 (en) * 2008-11-11 2010-05-20 Danisco Us Inc. Compositions and methods comprising a subtilisin variant
RU2560978C2 (ru) * 2008-11-11 2015-08-20 ДАНИСКО ЮЭс ИНК. Протеазы, содержащие одну или несколько комбинируемых мутаций
EP2424981A2 (de) * 2009-04-30 2012-03-07 Kao Corporation Alkalische-protease-varianten
DE102009029513A1 (de) * 2009-09-16 2011-03-24 Henkel Ag & Co. Kgaa Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Proteasen
CA2798451C (en) * 2010-05-06 2020-10-06 Danisco Us Inc. Compositions and methods comprising subtilisin variants with t022a-e271f substitutions
DE102010028951A1 (de) 2010-05-12 2011-11-17 Henkel Ag & Co. Kgaa Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Protease und Lipase
DE102010063457A1 (de) * 2010-12-17 2012-06-21 Henkel Ag & Co. Kgaa Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Protease und Cellulase
DE102010063458A1 (de) * 2010-12-17 2012-06-21 Henkel Ag & Co. Kgaa Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Protease und Amylase
AR086281A1 (es) * 2011-05-05 2013-12-04 Danisco Us Inc Composiciones y metodos que comprenden variantes de serina proteasas
EP2710105A4 (de) 2011-05-20 2015-03-25 Ecolab Usa Inc Phosphatfreie reinigungsmittel und phosphatfreie säuren in einem alternierenden alkali/säuresystem zur reinigung von artikeln
CA2856820C (en) 2011-05-20 2019-10-29 Ecolab Usa Inc. Acid formulations for use in a system for warewashing
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DE102012201522A1 (de) * 2012-02-02 2013-08-08 Basf Se Lagerstabiles flüssiges Geschirrspülmittel enthaltend Protease und Amylase
ES2582608T3 (es) * 2012-02-17 2016-09-14 Henkel Ag & Co. Kgaa Composiciones detergentes que comprenden variantes de subtilasa
DE102012215107A1 (de) * 2012-08-24 2014-02-27 Basf Se Festes Geschirrspülmittel mit verbesserter Proteaseleistung
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CN103013960A (zh) * 2012-12-21 2013-04-03 青岛蔚蓝生物集团有限公司 一种碱性蛋白酶及其重组表达工程菌
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JP6254693B2 (ja) 2013-10-24 2017-12-27 エコラボ ユーエスエー インコーポレイティド 表面から汚れを除去する組成物及び方法
US10626388B2 (en) 2014-07-04 2020-04-21 Novozymes A/S Subtilase variants and polynucleotides encoding same
RU2710720C2 (ru) * 2014-12-04 2020-01-10 Новозимс А/С Варианты субтилазы и кодирующие их полинуклеотиды
EP4219704A3 (de) * 2015-05-13 2023-08-23 Danisco US Inc Aprl-clade-proteasevarianten und verwendungen davon
CN108291179B (zh) 2015-11-25 2020-07-07 荷兰联合利华有限公司 液体洗涤剂组合物
CA3026064A1 (en) * 2016-05-31 2017-12-07 Danisco Us Inc. Protease variants and uses thereof
US20190185836A1 (en) * 2016-09-02 2019-06-20 The Regents Of The University Of California Engineered subtiligase variants for versatile, site-specific labeling of proteins
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Also Published As

Publication number Publication date
ATE284962T1 (de) 2005-01-15
CZ289323B6 (cs) 2002-01-16
KR100344976B1 (ko) 2002-11-23
CN1133068A (zh) 1996-10-09
DE69434187T2 (de) 2005-12-15
EP0723590A1 (de) 1996-07-31
RU2136756C1 (ru) 1999-09-10
DK0723590T3 (da) 2005-04-25
HK1000649A1 (en) 2005-06-03
FI119697B (fi) 2009-02-13
ES2235164T3 (es) 2005-07-01
FI961631A0 (fi) 1996-04-12
FI961631A (fi) 1996-04-15
MA23346A1 (fr) 1995-04-01
EP1526182A3 (de) 2005-05-04
JP4086207B2 (ja) 2008-05-14
PL178478B1 (pl) 2000-05-31
NO961468D0 (no) 1996-04-12
CN1056649C (zh) 2000-09-20
AU8015794A (en) 1995-05-04
EP0723590B1 (de) 2004-12-15
NO961468L (no) 1996-04-12
US20030077807A1 (en) 2003-04-24
EP1526182A2 (de) 2005-04-27
TW448230B (en) 2001-08-01
PT723590E (pt) 2005-04-29
WO1995010615A1 (en) 1995-04-20
PL313942A1 (en) 1996-08-05
CA2173973A1 (en) 1995-04-20
NZ274998A (en) 1997-04-24
US7250281B2 (en) 2007-07-31
CZ106596A3 (en) 1996-09-11
US20040023353A1 (en) 2004-02-05
BR9407825A (pt) 1997-05-06
US6586221B2 (en) 2003-07-01
CA2173973C (en) 2009-10-06
NO325568B1 (no) 2008-06-23
AU700373B2 (en) 1999-01-07
ZA948086B (en) 1995-06-07
JPH09504170A (ja) 1997-04-28

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