FI116298B - Syntetisering av adipinsyra från kolkällor som härledds ur biomassa - Google Patents

Syntetisering av adipinsyra från kolkällor som härledds ur biomassa Download PDF

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Publication number
FI116298B
FI116298B FI961234A FI961234A FI116298B FI 116298 B FI116298 B FI 116298B FI 961234 A FI961234 A FI 961234A FI 961234 A FI961234 A FI 961234A FI 116298 B FI116298 B FI 116298B
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cis
adipic acid
acid
catechol
synthase
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FI961234A
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Finnish (fi)
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FI961234A (sv
FI961234A0 (sv
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John W Frost
Karen M Draths
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Purdue Research Foundation
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/11—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of two atoms of oxygen (1.13.11)
    • C12Y113/11001—Catechol 1,2-dioxygenase (1.13.11.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/852—Klebsiella

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
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  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
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  • Microbiology (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Claims (10)

1. Heterolog E. coli-värdcelltransformant, som förmär använda en i ett odlingsmedium befintlig kolkalla, 5 varvid värdcelltransformanten har en endogen biosyntesväg gemensam för aromatiska aminosyror, kännetecknad av att celltransformanten uttrycker heterologa strukturgener, som kodar 3-dehydroshikimatdehydratas och protokatechuat-dekarboxylas, vilka är endogena i förhällande tili Klebsi- 10 elia pneumoniae, och katekol-1,2-dioxigenas, som är endogen i förhällande tili Äcinetobacter calcoaceticus.
2. Heterolog celltransformant enligt patentkrav 1, kännetecknad av att den är ytterligare transformerad med uttryckbara rekombinanta DNA-sekvenser, som kodar en- 15 zymer transketolas, 3-deoxi-D-arabino-heptulosonat-7-fosfat-syntas (DAHP-syntas) och 3-dehydrokinatsyntas (DHQ-syntas).
3. Heterolog celltransformant enligt patentkrav 2, kännetecknad av att nämnda värdcell väljs bland säda-na mutantcellinjer, som uppvisar mutationer, vilka blocke- 20 rar 3-dehydroshikimatets omvandling tili korismat pä aromatiska aminosyrors gemensamma biosyntesväg.
: 4. Heterolog celltransformant enligt patentkrav 1, V kännetecknad av att värdcellen väljs bland sädana mu- tantcellinjer, som innehäller mutationer, vilka blockerar ··; 25 3-dehydroshikimatets omvandling tili korismat pä aromatis- : ka aminosyrors gemensamma biosyntesväg.
. 5. Förfarande för framställning av adipinsyra, kännetecknat av att nämnda förfarande omfattar steg, . där en transformerad E. Coli-bakteriecell odlas, som ut- 30 trycker heterologa strukturgener, som kodar 3-dehyd-roshikimatdehydratas och protokatechuatdekarboxylas, vilka • är endogena i förhällande till Klebsiella pneumoniae, och katekol-1,2-dioxigenas, som är endogen i förhällande tili t Äcinetobacter calcoaceticus, i ett medium, som innehäller * I » 35 en kolkälla, som omvandlas tili 3-dehydroshikimat av enzy-mer pä den gemensamma biosyntesvägen för nämnda cells aro- 116298 matiska aminosyror, sä att cis,cis-mukonsyra produceras genom 3-dehydroshikimatets biokatalytiska omvandling, och cis,cis-mukonsyran reduceras till adipinsyra.
6. Förfarande för framställning av adipinsyra en-5 ligt patentkrav 5, kännetecknat av att den transfor- merade värdcellen ytterligare uttrycker transketolas, 3- deoxi-D-arajbino-heptulosonat-7-fosfatsyntas (DAHP-syntas) och 3-dehydrokinatsyntas (DHQ-syntas).
7. Förfarande för framställning av adipinsyra en-10 ligt patentkrav 6, kännetecknat av att värdcellen väljs bland sädana mutantcellinjer, som pä aromatiska ami-nosyrors gemensamma biosyntesväg innehäller mutationer, vilka blockerar 3-dehydroshikimatets omvandling tili ko-rismat. 15
8. Förfarande för framställning av adipinsyra en- ligt patentkrav 7, kännetecknat av att celltransfor-manten odlas under förhällanden, som möjliggör kolkällans omvandling tili cis,cis-mukonsyra, och cis,cis-mukonsyran hydreras tili adipinsyra. 20
9. Förfarande enligt patentkrav 8, känneteck nat av att cis,cis-mukonsyra framställs pä en hastighet : som överskrider 0,35 mmol/l/h.
10. Heterolog celltransformant enligt patentkrav 3, kännetecknad av att mutationen är aroE. * • * t »
FI961234A 1993-09-16 1996-03-15 Syntetisering av adipinsyra från kolkällor som härledds ur biomassa FI116298B (sv)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US08/122,920 US5487987A (en) 1993-09-16 1993-09-16 Synthesis of adipic acid from biomass-derived carbon sources
US12292093 1993-09-16
US9410263 1994-09-02
PCT/US1994/010263 WO1995007996A1 (en) 1993-09-16 1994-09-02 Synthesis of adipic acid from biomass-derived carbon sources

Publications (3)

Publication Number Publication Date
FI961234A FI961234A (sv) 1996-03-15
FI961234A0 FI961234A0 (sv) 1996-03-15
FI116298B true FI116298B (sv) 2005-10-31

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US (2) US5487987A (sv)
EP (1) EP0722504A4 (sv)
JP (1) JPH09505463A (sv)
AU (1) AU687874B2 (sv)
CA (1) CA2171327C (sv)
FI (1) FI116298B (sv)
NZ (1) NZ273231A (sv)
WO (1) WO1995007996A1 (sv)

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