FI113787B - Vektor för expression av genprodukt i cellen - Google Patents
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- FI113787B FI113787B FI970674A FI970674A FI113787B FI 113787 B FI113787 B FI 113787B FI 970674 A FI970674 A FI 970674A FI 970674 A FI970674 A FI 970674A FI 113787 B FI113787 B FI 113787B
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Claims (12)
1. En vektor för expressionen av en önskad genprodukt med terapeutisk använd- barhet i en cell, kännetecknat av att innefattar en strukturell gen, som kodar den önskade genprodukten, manövrerbart kopplad tili en promotor under kontroll av ett DNA-repressorelement, som interagerar med en cellcykelspecifik repressor, för att reglera genexpression pä ett cellcykelspecifikt sätt.
2. En vektor enligt patentkrav 1, kännetecknat av att promotorn vidare kontrol- leras av ett vävnads- eller celltypspecifikt reglerande element.
3. En vektor enligt patentkrav 2, kännetecknat av att det vävnads- eller celltyp-specifika reglerande element är en enhanser.
4. En vektor enligt patentkrav 2 eller 3, kännetecknat av att det reglerande ele-15 ment är en enhanser, som företrädesvis kan aktiveras med glutaminrika aktivatorer.
5. En vektor enligt nägot av de föregäende patentkraven, kännetecknat av att DNA-repressorelement innefattar minst en del av sekvensen 5’-GCTGGCGGAA- : ’ GGTTTGAATGG-3’, eller en funktionellt likvärdig mutant eller homolog därav.
6. En vektor enligt nägot av de föregäende patentkraven, kännetecknat av att . ··. 20 DNA-repressorelement innefattar minst en del av sekvensen 5’-GCTGGCGGAA- " GGTTTGAATGG-3’ och ett transkriptionsinitieringsställe säsom det första huvud- sakligt transkriptionsinitieringsstället hos cdc25C-genen, följt av genen som kodar den önskade genprodukten.
7. En vektor enligt nägot av de föregäende patentkraven, kännetecknat av att » » . 1 · ·. 25 DNA-repressorelement är härlett frän en cellcykelreglerad gen.
8. En vektor enligt nägot av de föregäende patentkraven, kännetecknat av att :··· DNA-repressorelement härleds frän cdc25C-genen, cdc2-genen eller cyklin A-ge- *. nen.
9. En vektor enligt nägot av de föregäende patentkraven, kännetecknat av att 30 genprodukten är cytotoxisk eller cytostatisk. 113787
10. En vektor enligt nägot av patentkraven 1 tili 8, kännetecknat av att genpro-dukten är ett prodrugaktiverande enzym.
11. En vektor enligt patentkrav 10, kännetecknat av att prodrugen efter aktive-ring har allmän cytotoxisk eller cytostatisk aktivitet.
12. En vektor enligt nägot av patentkraven 1 till 11, för användning inom medici- nen. 1
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GBGB9506466.3A GB9506466D0 (en) | 1994-08-26 | 1995-03-29 | Cell cycle regulated repressor and dna element |
GB9502000 | 1995-08-23 | ||
PCT/GB1995/002000 WO1996006943A1 (en) | 1994-08-26 | 1995-08-23 | Cell cycle regulated repressor and dna element |
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FI970674A FI113787B (sv) | 1994-08-26 | 1997-02-18 | Vektor för expression av genprodukt i cellen |
FI970768A FI970768A (sv) | 1994-08-26 | 1997-02-24 | Genterapeutisk behandling av tumörer med ett endotelcellspecifikt aktivt ämne som är beroende av cellcykeln |
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FI970768A FI970768A (sv) | 1994-08-26 | 1997-02-24 | Genterapeutisk behandling av tumörer med ett endotelcellspecifikt aktivt ämne som är beroende av cellcykeln |
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US6750329B1 (en) * | 1989-05-05 | 2004-06-15 | Research Development Foundation | Antibody delivery system for biological response modifiers |
US6710174B2 (en) * | 2001-09-13 | 2004-03-23 | Isis Pharmaceuticals, Inc. | Antisense inhibition of vascular endothelial growth factor receptor-1 expression |
ATE198492T1 (de) * | 1994-08-26 | 2001-01-15 | Aventis Pharma Gmbh | Gentherapie von erkrankungen, die durch das immunsystem bedingt sind, mit hilfe eines zellspezifischen zellzyklusregulierten wirkstoffes |
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