FI109029B - Förfarande för framställning av ett ytprotein, som finns i stammar av Haemophilus influenzae eller till denna relaterade Haemophilus arter - Google Patents
Förfarande för framställning av ett ytprotein, som finns i stammar av Haemophilus influenzae eller till denna relaterade Haemophilus arter Download PDFInfo
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- FI109029B FI109029B FI925460A FI925460A FI109029B FI 109029 B FI109029 B FI 109029B FI 925460 A FI925460 A FI 925460A FI 925460 A FI925460 A FI 925460A FI 109029 B FI109029 B FI 109029B
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- protein
- igd
- variants
- haemophilus
- influenzae
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- A61K39/102—Pasteurellales, e.g. Actinobacillus, Pasteurella; Haemophilus
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- C07K14/285—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pasteurellaceae (F), e.g. Haemophilus influenza
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- C—CHEMISTRY; METALLURGY
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- C07K16/1203—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria
- C07K16/1242—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria from Pasteurellaceae (F), e.g. Haemophilus influenza
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- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
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Claims (20)
1. Förfarande for framställning av ett ytprotein, som firms i flera stammar av Haemophilus influenzae eller till denna relaterade Haemophilus arter, med en synlig 5 molekylvikt pä 42000 och förmäga att binda mänskligt IgD och som har en DNA sekvens, som beskrivits i figur 9, eller naturligt förekommande eller artificiellt modifierade varianter av denna med samma function som nämnda protein, eller en immunogen eller IgD -bindande del av nämnda protein eller varianter, k ä n n e t e c k n a d därav, att i förfarandet nämnda ytprotein isoleras frän en bakterie cell som 10 uttrycker proteinet och frän odlingsmediet och renas.
2. Plasmid eller fag, kännetecknad därav, att plasmid eller fag som innehäller en genetisk kod för ett protein av Haemophilus influenzae eller till denna relaterade Haemophilus arter, varvid nämnda protein har en synlig molekylvikt pä 15 42000, förmaga att binda mänskligt IgD och nämnda protein har en DNA sekvens som beskrivits i figur 9, eller för naturligt förekommande eller artificiellt modifierade varianter av denna, med samma function som nämnda protein, eller för en immunogen ' · · ·' eller IgD -bindande del av nämnda protein eller varianter. ;"; 20 3. Icke-mänsklig värd, kännetecknad därav, att den innehäller en plasmid Τ'. eller en fag som defmierats i patentkrav 2 och har förmäga att producera nämnda ’ protein eller varianter av detta eller en del av nämnda protein eller av varianter, vilken värd väljs bland bakterier, jäster och växter. . · · *. 25 4. Värd enligt patentkrav 3, kännetecknad därav, att den är E. coli. • *
5. Ett DNA segment, kännetecknat därav, att det innefattar en DNA sekvens * t som kodar ett protein av Haemophilus influenzae eller av relaterade Haemophilus T;' arter, som har en DNA sekvens som beskrivits i figur 9, varvid nämnda protein har 30 en synlig molekylvikt pä 42000 och förmäga att binda mänskligt IgD, eller naturligt förekommande eller artificiellt modifierade varianter av denna med samma function i 30 109029 som nämnda protein, eller en immunogen eller IgD -bindande del av nämnda protein eller varianter av detta.
6. Rekombinant DNA molekyl, kännetecknad därav, att den innehäller en 5 nukleotidsekvens som kodar för ett ytprotein av Haemophilus influenzae eller till denna relaterade Haemophilus arter, varvid nämnda protein har en synlig molekylvikt pä 42000 och förmäga att binda mänskligt IgD, och har en DNA sekvens som beskrivits i figur 9, eller för naturligt förekommande eller artificiellt modifierade varianter av denna med samma function som nämnda protein, eller för en immunogen 10 eller IgD -bindande del av nämnda protein eller varianter, vilken nukleotidsekvens är förenad med en annan gen.
7. Plasmid eller fag, kännetecknad därav, att den innehäller en förenad nukleotidsekvens enligt patentkrav 6. 15
8. Icke-mänsklig värd, kännetecknad därav, att den innehäller ätminstone en plasmid eller fag enligt patentkrav 7, vilken värd väljes bland bakterier, jäster eller " · · · ’ växter. * · · « « · « · · '.“I 20 9. Värd enligt patentkrav 8, kännetecknad därav, att den är E. coli. ♦ » · • « * · • ·
10. Förfarande för framställning av ett förenat protein eller förenad polypeptid, kännetecknat därav, att ett ytprotein av Haemophilus influenzae eller av relaterade Haemophilus arter, varvid nämnda protein har en synlig molekylvikt pä .' · *. 25 42000 och förmäga att binda mänskligt IgD och har en DNA sekvens som beskrivits .' , i figur 9, eller naturligt förekommande eller artificiellt modifierade varianter av denna 1 med samma function som nämnda protein, eller en immunogen eller IgD -bindande del av nämnda protein eller varianter, kombineras med ett annat protein genom att • » · ’,,; använda en rekombinant DNA molekyl enligt patentkrav 6. 30
13. Förfarande för framställning av en fusionsprodukt, kännetecknat därav, 31 109029 att i fusionsprodukt ett ytprotein av Haemophilus influenzae eller av relaterade Haemophilus arter, varvid nämnda protein har en synlig molekylvikt pä 42000 och förmäga att binda mänskligt IgD och har en DNA sekvens som beskrivits i figur 9, eller naturligt förekommande eller artificiellt modifierade varianter av denna med 5 samma function som nämnda protein, eller en immunogen eller IgD-bindande del av nämnda protein eller varianter, bindas pä kovalent sätt eller pä annat sätt till ett protein, kolhydrat eller matris.
12. Förfarande för framställning av ett vaccin som innehäller ett ytprotein av Hae-10 mophilus influenzae eller av relaterade Haemophilus arter, varvid nämnda protein har en synlig molekylvikt pä 42000 och förmäga att binda mänskligt IgD och har en DNA sekvens som beskrivits i figur 9, eller naturligt förekommande eller artificiellt modifierade varianter av denna med samma function som nämnda protein, eller en immunogen eller IgD -bindande del av denna, kännetecknat därav, att i 15 förfarandet nämnda ytprotein eller varianter eller del av denna inkluderas i vaccinet.
13. Förfarande för framställning av ett vaccin som innehäller ett ytprotein av Hae- ' · · ·' mophilus influenzae eller av relaterade Haemophilus arter, varvid nämnda protein har ’ * ‘ · * en synlig molekylvikt pä 42000 och förmäga att binda mänskligt IgD och har en DNA ;··; 20 sekvens som beskrivits i figur 9, eller naturligt förekommande eller artificiellt • · · ’ I * * modifierade varianter av denna med samma function som nämnda protein, eller en !..* immunogen eller IgD -bindande del av denna, kännetecknat därav, att i förfarandet nämnda ytprotein eller varianter eller del av denna inkluderas i vaccinet _ och vaccinet kombineras med ett annat vaccin. .···. 25 . 14. Förfarande för framställning av ett vaccin som innehäller ett ytprotein av Hae- ! mophilus influenzae eller av relaterade Haemophilus arter, varvid nämnda protein har en synlig molekylvikt pä 42000 och förmäga att binda mänskligt IgD och har en sekvens som beskrivits i figur 9, eller naturligt förekommande eller artificiellt 30 modifierade varianter av denna med samma function som nämnda protein, eller en immunogen eller IgD -bindande del av denna, kännetecknat därav, att i 32 109029 förfarandet nämnda ytprotein eller varianter eller del av derma inkluderas i vaccinet och vaccinet kombineras med en immunogen del av en annan molekyl.
15. En hybridomcell med förmäga att producera en monoklonal antikropp tili en 5 immunogen del av ett ytprotein av Haemophilus influenzae eller av relaterade Haemophilus arter, varvid nämnda protein har en synlig molekylvikt pä 42000 och förmäga att binda mänskligt IgD och har en DNA sekvens som beskrivits i figur 9.
16. Förfarande för framställning av en renad antikropp som är specifik för en 10 immunogen del av ytprotein av Haemophilus influenzae eller av relaterade Haemophilus arter, varvid nämnda protein har en synlig molekylvikt pä 42000 och förmäga att binda mänskligt IgD och har en DNA sekvens som beskrivits i figur 9, kännetecknat därav, att i förfarandet hybrider som producerar antikroppar tili nämnda ytprotein förenas, klonas och odlas därefter i paslig medium. 15
17. Förfarande för detektering av närvaron av Haemophilus influenzae eller av relaterade Haemophilus arter i ett prov, kännetecknat därav, att nämnda '···' prov bringas i kontakt med en antikropp enligt patentkrav 16 i närvaro av en indikator. —: 20 • * * » # '1*1 18. Förfarande för att detektera närvaron av Haemophilus influenzae eller av relatera- 1..' de Haemophilus arter i ett prov, kännetecknat därav, att nämnda prov bringas i kontakt med ett DNA prov eller en primer som konstruerats att svara mot ____: nukleinsyror som kodar för ett ytprotein av Haemophilus influenzae eller av proteiner . - · *. 25 med avtäckt yta av relaterade Haemophilus arter, varvid nämnda protein har en synlig .* , molekylvikt pä 42000 och en förmäga att binda mänskligt IgD och har en DNA 1 sekvens som bestkrivits i figur 9, eller för naturligt förekommande eller artificiellt . modifierade varianter av derma med samma function som nämnda protein, eller för f » I • ’ en immunogen eller IgD -bindande del av nämnda protein eller varianter. 30
19. Förfarande för detektering av IgD, kännetecknat därav, att ett ytprotein 33 109029 av Haemophilus influenzae eller till denna relaterade Haemophilus arter används, varvid nämnda protein har en synlig molekylvikt pä 42000 och förmäga att binda mänskligt IgD och har en DNA sekvens som beskrivits i figur 9, eller av naturligt förekommande eller artificiellt modifierade varianter av denna, med samma function 5 som nämnda protein, eller av en immunogen eller IgD -bindande del av nämnda protein eller varianter valbart märkt och/eller bundet till en matris.
20. Förfarande för separering av IgD, kännetecknat därav, att ett ytprotein av Haemophilus influenzae eller till denna relaterade Haemophilus arter används, 10 varvid nämnda protein har en synlig molekylvikt pä 42000 och förmäga att binda mänskligt IgD och har en DNA sekvens som beskrivits i figur 9, eller av naturligt förekommande eller artificiellt modifierade varianter av denna, med samma function som nämnda protein, eller av en immunogen eller IgD -bindande del av nämnda protein eller varianter valbart bundet till en matris. 15 t t * » * * · · i
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE9001949A SE466259B (sv) | 1990-05-31 | 1990-05-31 | Protein d - ett igd-bindande protein fraan haemophilus influenzae, samt anvaendning av detta foer analys, vacciner och uppreningsaendamaal |
| SE9001949 | 1990-05-31 | ||
| PCT/SE1991/000129 WO1991018926A1 (en) | 1990-05-31 | 1991-02-21 | PROTEIN D - AN IgD-BINDING PROTEIN OF HAEMOPHILUS INFLUENZAE |
| SE9100129 | 1991-02-21 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI925460A FI925460A (fi) | 1992-11-30 |
| FI925460A0 FI925460A0 (fi) | 1992-11-30 |
| FI109029B true FI109029B (sv) | 2002-05-15 |
Family
ID=20379641
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI925460A FI109029B (sv) | 1990-05-31 | 1992-11-30 | Förfarande för framställning av ett ytprotein, som finns i stammar av Haemophilus influenzae eller till denna relaterade Haemophilus arter |
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| US (9) | US5989828A (sv) |
| EP (1) | EP0594610B1 (sv) |
| JP (1) | JP3066072B2 (sv) |
| AT (1) | ATE170531T1 (sv) |
| AU (1) | AU650011B2 (sv) |
| CA (1) | CA2083172C (sv) |
| DE (3) | DE69130116T2 (sv) |
| DK (1) | DK0594610T3 (sv) |
| ES (1) | ES2119776T3 (sv) |
| FI (1) | FI109029B (sv) |
| LU (2) | LU91609I2 (sv) |
| NL (2) | NL300409I2 (sv) |
| NO (4) | NO309721B1 (sv) |
| SE (1) | SE466259B (sv) |
| WO (1) | WO1991018926A1 (sv) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| SE466259B (sv) * | 1990-05-31 | 1992-01-20 | Arne Forsgren | Protein d - ett igd-bindande protein fraan haemophilus influenzae, samt anvaendning av detta foer analys, vacciner och uppreningsaendamaal |
| SE9201331D0 (sv) * | 1992-04-28 | 1992-04-28 | Hightech Receptor C O Active | Protein l och hybridproteiner daerav |
| US6153406A (en) * | 1993-07-23 | 2000-11-28 | North American Vaccine, Inc. | Method for the high level expression, purification and refolding of the outer membrane protein P2 from Haemophilus influenzae type B |
| US6001564A (en) * | 1994-09-12 | 1999-12-14 | Infectio Diagnostic, Inc. | Species specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
| US20020055101A1 (en) | 1995-09-11 | 2002-05-09 | Michel G. Bergeron | Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
| NZ306316A (en) * | 1995-04-17 | 2001-06-29 | Smithkline Beecham Biolog S | use of lipoprotein and a type 2T independent antigen to induce an immune response to the antigen |
| US5994066A (en) * | 1995-09-11 | 1999-11-30 | Infectio Diagnostic, Inc. | Species-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
| US20100267012A1 (en) | 1997-11-04 | 2010-10-21 | Bergeron Michel G | Highly conserved genes and their use to generate probes and primers for detection of microorganisms |
| US20030049636A1 (en) | 1999-05-03 | 2003-03-13 | Bergeron Michel G. | Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories |
| US20050033022A1 (en) * | 1997-09-26 | 2005-02-10 | Smithkline Beecham Biologicals Sa | Fusion proteins comprising HIV-1 Tat and/or Nef proteins |
| GB9727262D0 (en) * | 1997-12-24 | 1998-02-25 | Smithkline Beecham Biolog | Vaccine |
| CZ298347B6 (cs) | 1998-02-05 | 2007-09-05 | Glaxosmithkline Biologicals S.A. | Fúzní protein z rodiny MAGE, kódová sekvence nukleové kyseliny, vektor, hostitelská bunka, vakcina a použití fúzního proteinu pro výrobu vakciny |
| US6518256B1 (en) | 1998-03-18 | 2003-02-11 | Corixa Corporation | Compounds and methods for therapy and diagnosis of lung cancer |
| US6312695B1 (en) | 1998-03-18 | 2001-11-06 | Corixa Corporation | Compounds and methods for therapy of lung cancer |
| US6531315B1 (en) | 1998-03-18 | 2003-03-11 | Corixa Corporation | Compositions and methods for the therapy and diagnosis of lung cancer |
| US6426072B1 (en) | 2000-08-02 | 2002-07-30 | Corixa Corporation | Compositions and methods for the therapy and diagnosis of lung cancer |
| US6706262B1 (en) | 1998-03-18 | 2004-03-16 | Corixa Corporation | Compounds and methods for therapy and diagnosis of lung cancer |
| US6660838B1 (en) | 1998-03-18 | 2003-12-09 | Corixa Corporation | Compounds and methods for therapy and diagnosis of lung cancer |
| US20020147143A1 (en) | 1998-03-18 | 2002-10-10 | Corixa Corporation | Compositions and methods for the therapy and diagnosis of lung cancer |
| US6482597B1 (en) | 1999-12-17 | 2002-11-19 | Corixa Corporation | Compounds and methods for therapy and diagnosis of lung cancer |
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| US3800798A (en) * | 1972-07-11 | 1974-04-02 | A Winkler | Hydrophobic catheter construction |
| DE2322533C2 (de) * | 1972-11-06 | 1986-08-28 | Pharmacia AB, Uppsala | Verfahren zum Binden von Immunoglobulin und Hilfsmittel zur Durchführung des Verfahrens |
| SE451596B (sv) * | 1983-06-22 | 1987-10-19 | Excorim Kb | Forfarande for utvinning av protein g |
| SE459503B (sv) * | 1985-05-03 | 1989-07-10 | Excorim Kommanditbolag | Hybrid-dna-molekyl innefattande dna-sekvens som kodar foer protein g samt foerfarande foer framstaellning av protein g |
| SE459662B (sv) * | 1986-03-21 | 1989-07-24 | Pharmacia Ab | Hybrid-dna-molekyl som kodar foer ett protein med samma igg specificitet som protein g, plasmid innehaallande densamma samt foerfarande foer framstaellning av proteinet |
| US5173294A (en) * | 1986-11-18 | 1992-12-22 | Research Foundation Of State University Of New York | Dna probe for the identification of haemophilus influenzae |
| AU623353B2 (en) * | 1987-12-10 | 1992-05-14 | Board Of Regents, The University Of Texas System | Methods and compositions for the production of haemophilus influenzae type b major outer membrane protein antigens |
| ATE105189T1 (de) * | 1988-04-19 | 1994-05-15 | American Cyanamid Co | Haemophilus influenzae typ b polysaccharidaussermembranprotein-konjugat als impfstoff. |
| USRE37919E1 (en) | 1989-05-12 | 2002-12-03 | The General Hospital Corporation | Recombinant DNA method for production of parathyroid hormone |
| SE466259B (sv) | 1990-05-31 | 1992-01-20 | Arne Forsgren | Protein d - ett igd-bindande protein fraan haemophilus influenzae, samt anvaendning av detta foer analys, vacciner och uppreningsaendamaal |
| US6680057B1 (en) | 1995-03-23 | 2004-01-20 | Immunex Corporation | Methods of treating autoimmune disease by administering interleukin-17 receptor |
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1990
- 1990-05-31 SE SE9001949A patent/SE466259B/sv not_active IP Right Cessation
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- 1991-02-21 DE DE69130116T patent/DE69130116T2/de not_active Expired - Lifetime
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- 1991-02-21 EP EP91907067A patent/EP0594610B1/en not_active Expired - Lifetime
- 1991-02-21 WO PCT/SE1991/000129 patent/WO1991018926A1/en active IP Right Grant
- 1991-02-21 JP JP3506522A patent/JP3066072B2/ja not_active Expired - Lifetime
- 1991-02-21 DE DE122009000059C patent/DE122009000059I1/de active Pending
- 1991-02-21 DE DE200912000060 patent/DE122009000060I1/de active Pending
- 1991-02-21 AT AT91907067T patent/ATE170531T1/de active
- 1991-02-21 ES ES91907067T patent/ES2119776T3/es not_active Expired - Lifetime
- 1991-02-21 DK DK91907067T patent/DK0594610T3/da active
- 1991-02-21 AU AU75593/91A patent/AU650011B2/en not_active Expired
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1992
- 1992-11-24 NO NO924507A patent/NO309721B1/no not_active IP Right Cessation
- 1992-11-30 FI FI925460A patent/FI109029B/sv active Protection Beyond IP Right Term
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1996
- 1996-11-12 US US08/747,381 patent/US5989828A/en not_active Expired - Fee Related
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1997
- 1997-09-25 US US08/936,912 patent/US5888517A/en not_active Expired - Fee Related
- 1997-11-05 US US08/968,885 patent/US5858677A/en not_active Expired - Lifetime
- 1997-11-13 US US08/969,761 patent/US6025484A/en not_active Expired - Fee Related
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1999
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2000
- 2000-06-30 US US09/607,933 patent/US7115271B1/en not_active Ceased
- 2000-07-20 NO NO20003716A patent/NO322330B1/no not_active IP Right Cessation
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- 2006-09-15 US US11/521,598 patent/US7666621B2/en not_active Expired - Fee Related
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- 2008-10-02 US US12/285,368 patent/USRE41277E1/en not_active Expired - Fee Related
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- 2009-09-24 NO NO2009022C patent/NO2009022I1/no not_active Application Discontinuation
- 2009-09-24 NL NL300409C patent/NL300409I2/nl unknown
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- 2009-09-24 LU LU91610C patent/LU91610I2/fr unknown
- 2009-09-24 NO NO2009021C patent/NO2009021I1/no not_active Application Discontinuation
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