FI108944B - Kloning och uttryckning av xylanasgener av svamphärstamning - Google Patents

Kloning och uttryckning av xylanasgener av svamphärstamning Download PDF

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Publication number
FI108944B
FI108944B FI921231A FI921231A FI108944B FI 108944 B FI108944 B FI 108944B FI 921231 A FI921231 A FI 921231A FI 921231 A FI921231 A FI 921231A FI 108944 B FI108944 B FI 108944B
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FI
Finland
Prior art keywords
dna sequence
xylanase
dna
gene
expression
Prior art date
Application number
FI921231A
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English (en)
Finnish (fi)
Other versions
FI921231A0 (fi
FI921231A (fi
Inventor
Ooyen Albert Johannes Jose Van
Graaff Leendert Hendrik De
Jacob Visser
Den Broeck Henriette Catha Van
Abraham Harder
Jan Dirk Rene Hille
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Dsm Nv
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8205086&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=FI108944(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dsm Nv filed Critical Dsm Nv
Publication of FI921231A0 publication Critical patent/FI921231A0/fi
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Publication of FI108944B publication Critical patent/FI108944B/sv

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D8/00—Methods for preparing or baking dough
    • A21D8/02—Methods for preparing dough; Treating dough prior to baking
    • A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
    • A23K30/10—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder
    • A23K30/15—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging
    • A23K30/18—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01032—Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase

Claims (15)

1. Renad och isolerad DNA-sekvens, som kodar för en polypeptid med xylanasaktivitet, kännetecknad därav, att 5 DNA-sekvensen är a) en DNA-sekvens härstammande frän svamp som sträcker sig frän positionen 1030 till positionen 1634 i fig. 8, b) en DNA-sekvens som förmär hybridiseras med DNA-sekvensen i föregäende punkt a) eller med en sond, som 10 innehäller nukleotider frän position 749 till position 1706 av DNA-sekvensen enligt fig. 8, i hybridiseringsförhällanden, som motsvarar förhällanden, vid vilke tvä tvättningar i 5 x SSC, 0,1 % SDS vid 60 °C I och tvä tvättningar i 3 x SSC vid 60 °C utförs, eller 15 c) en DNA-sekvens, som skiljer sig frän DNA-sekvensen enligt punkten a) eller b) endast till följd av degeneration av den genetiska koden; och vidare därav, att DNA-sekvensen inte är den följande DNA-sekvensen visad i patentansökan FI 92 5730: 20 ATGAAGGTCA CTGCGGCTTT TGCAGGTCTT TTGGTCACGG CATTCGCCGC TCCTGTGCCG GAACCTGTTC TGGTGTCGCG AAGTGCTGGT ATTAACTACG :·*: TGCAAAACTA CAACGGCAAC CTTGGTGATT TCACCTATGA CGAGAGTGCC GGAACATTTT CCATGTACTG GGAAGATGGA GTGAGCTCCG ACTTTGTCGT ’’ TGGTCTGGGC TGGACCACTG GTTCTTCTAA GTGAGTGACT GTATTCTTTA ACCAAAGTCT AGGATCTAAC GTTTTCTAGC GCTATCACCT ACTCTGCCGA ·:·*: 25 ATACAGTGCT TCTGGCTCCT CTTCCTACCT CGCTGTGTAC GGCTGGGTCA .·. : ACTATCCTCA GGCTGAATAC TACATCGTCG AGGATTACGG TGATTACAAC I./’ CCTTGCAGCT CGGCCACAAG CCTTGGTACC GTGTACTCTG ATGGAAGCAC ; CTACCAAGTC TGCACCGACA CTCGAACTAA CGAACCGTCC ATCACGGGAA CAAGCACGTT CACGCAGTAC TTCTCCGTTC GAGAGAGCAC GCGCACATCT ....: GGAACGGTGA CTGTTGCCAA CCATTTCAAC TTCTGGGCGC AGCATGGGTT CGGAAATAGC GACTTCAATT ATCAGGTCAT GGCAGTGGAA GCATGGAGCG ···’’ 3 0 GTGCTGGCAG CGCCAGTGTC ACGATCTCCT CTTAA. I * * i · » · t
» * *:”· 2· DNA-sekvens enligt patentkravet 1, kännetecknad därav, : .*. att den härstammar frän en svamp av släktet Aspergillus. 74 108944
3. DNA-sekvens enligt patentkravet 1, kännetecknad därav, att den härstammar frän en svamp av släktet Trichoderma.
4. DNA-sekvens enligt patentkravet 1, kännetecknad därav, 5 att DNA-sekvensen härstammar frän arterna Aspergillus tubigensis, Aspergillus niger, Aspergillus awamori eller Aspergillus aculeatus.
5. DNA-sekvens enligt patentkravet 3, kännetecknad därav, 10 att DNA-sekvensen härstammar frän arten Trichoderma reesei.
6. DNA-konstruktion, kännetecknad därav, att den innehäller en DNA-sekvens enligt nägot av patenkraven 1-5 15 funktionellt sammanlänkad med regleringsregioner som förmär styra överuttryckningen av polypeptiden med xylanasaktivitet i en lämplig uttrycksvärd.
7. DNA-konstruktion enlig patentkravet 6, kännetecknad 20 ytterligare därav, att regleringsregionen innehäller en promotor, som härstammar frän en amyloglukosidasgen eller är en naturlig promotor för xylanasgenen. • · ·
8. DNA-konstruktion enlig patentkravet 7, kännetecknad ·;··· 25 ytterligare därav, att regleringsregionen innehäller en utsöndringsledarsekvens, som härstammar frän en amyloglukosidasgen eller är en naturlig utsöndringsledarsekvens för xylanasgenen. » · · ‘ : 30
9. Transformerad mikrobvärd, som förmär producera en .···, polypeptid med xylanasaktivitet, kännetecknad därav, att ♦ · mikrobvärden innehäller en uttrycskonstruktion enligt . *. nägot av patentkraven 6-8. • ·
FI921231A 1990-07-24 1992-03-20 Kloning och uttryckning av xylanasgener av svamphärstamning FI108944B (sv)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP90202020 1990-07-24
EP90202020 1990-07-24
NL9100137 1991-01-28
PCT/NL1991/000137 WO1992001793A1 (en) 1990-07-24 1991-07-24 Cloning and expression of xylanase genes from fungal origin

Publications (3)

Publication Number Publication Date
FI921231A0 FI921231A0 (fi) 1992-03-20
FI921231A FI921231A (fi) 1992-03-20
FI108944B true FI108944B (sv) 2002-04-30

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Application Number Title Priority Date Filing Date
FI921232A FI921232A (fi) 1990-07-24 1992-03-20 Enzymatisk behandling av ensilage.
FI921231A FI108944B (sv) 1990-07-24 1992-03-20 Kloning och uttryckning av xylanasgener av svamphärstamning

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Application Number Title Priority Date Filing Date
FI921232A FI921232A (fi) 1990-07-24 1992-03-20 Enzymatisk behandling av ensilage.

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US (1) US5358864A (sv)
EP (3) EP0468596B1 (sv)
JP (2) JPH05500907A (sv)
KR (1) KR100212232B1 (sv)
AT (2) ATE124844T1 (sv)
AU (2) AU647170B2 (sv)
CA (2) CA2067329A1 (sv)
DE (3) DE463706T1 (sv)
DK (2) DK0468596T3 (sv)
ES (1) ES2086267T3 (sv)
FI (2) FI921232A (sv)
HU (2) HU215234B (sv)
IE (3) IE68859B1 (sv)
IL (1) IL98941A (sv)
NO (2) NO307347B1 (sv)
NZ (2) NZ239083A (sv)
PL (2) PL291225A1 (sv)
PT (1) PT98419B (sv)
WO (2) WO1992001389A1 (sv)

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AU647170B2 (en) 1994-03-17
US5358864A (en) 1994-10-25
KR920702421A (ko) 1992-09-04
AU8320591A (en) 1992-02-18
JPH05501657A (ja) 1993-04-02
FI921231A0 (fi) 1992-03-20
ES2086267T1 (es) 1996-07-01
NO921133D0 (no) 1992-03-23
PL291226A1 (en) 1992-09-07
EP0463706B2 (en) 2010-01-27
CA2067329A1 (en) 1992-01-25
IL98941A (en) 2000-12-06
DK0463706T3 (da) 2003-06-23
NZ239085A (en) 1993-08-26
NO307347B1 (no) 2000-03-20
ATE124844T1 (de) 1995-07-15
JPH05500907A (ja) 1993-02-25
WO1992001793A1 (en) 1992-02-06
PT98419A (pt) 1992-06-30
NO921134D0 (no) 1992-03-23
FI921231A (fi) 1992-03-20
FI921232A0 (fi) 1992-03-20
DK0468596T3 (da) 1995-11-20
PL291225A1 (en) 1992-09-21
CA2066734A1 (en) 1992-01-25
PL171271B1 (pl) 1997-03-28
IE912583A1 (en) 1992-01-29
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HU215234B (hu) 1998-11-30
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DE69133201T2 (de) 2003-12-11
IE20040144A1 (en) 2004-05-19
DE69111148D1 (de) 1995-08-17
HU9200961D0 (en) 1992-06-29
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