FI105481B - Nya proteolytiska enzymer och deras användning i tvättmedel - Google Patents
Nya proteolytiska enzymer och deras användning i tvättmedel Download PDFInfo
- Publication number
- FI105481B FI105481B FI894799A FI894799A FI105481B FI 105481 B FI105481 B FI 105481B FI 894799 A FI894799 A FI 894799A FI 894799 A FI894799 A FI 894799A FI 105481 B FI105481 B FI 105481B
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- FI
- Finland
- Prior art keywords
- protease
- mutant
- detergent
- residue
- washing
- Prior art date
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- 0 *1C2=C[C@@]1CO2 Chemical compound *1C2=C[C@@]1CO2 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Detergent Compositions (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Claims (12)
1. Ett mutantproteas för använding i tvättroedel, känne-tecknat därav, att det har ätminstone 70 %:s homologi 5 tili aminosyrasekvensen av serinproteaset PB92, vilken amino- syrasekvens är följande: h2n-a-q-s-v-p-w-g-i-s-r-v-q-a-p-a-a-h-n-r-g-l-t-g-s-g-v-k- V-A-V-L-D-T-G-I-S-T-H-P-D-L-N-I-R-G-G-A-S-F-V-P-G-E-P-S-T-Q-D-G-N-G-H-G-T-H-V-A-G-T-I-A-A-L-N-N-S-I-G-V-L-G-V-A-10 P-N-A-E-L-Y-A-V-K-V-L-G-A-S-G-S-G-S-V-S-S-I-A-Q-G-L-E-W-A-G-N-N-G-M-H-V-A-N-L-S-L-G-S-P-S-P-S-A-T-L-E-Q-A-V-N-S-A-T-S-R-G-V-L-V-V-A-A-S-G-N-S-G-A-G-S-I-S-Y-P-A-R-Y-A-N-A-M-A-V-G-A-T-D-Q-N-N-N-R-A-S-F-S-Q-Y-G-A-G-L-D-I-V-A-P-G-V-N-V-Q-S-T-Y-P-G-S-T-Y-A-S-L-N-G-T-S-M-A-T-P-H-V-A“G-15 A-A-A-L-V-K-Q-K-N-P-S-W-S-N-V-Q-I-R-N-H-L-K-N-T-A-T-S-L-G-S-T-N-L-Y-G-S-G-L-V-N-A-E-A-A-T-R-COOH, att det skiljer sig frän nämnda PB92-serinproteas ätmins-tone i avseende pk en aminosyrarest i positioner, vilka motsvarar positionerna 99, 102, 116, 126, 127, 128, 130, 20 160, 203, 211, 212 och 216 av PB92-serinproteaset, att det har en förbättrad tvätteffekt och/eller en för-bättrad stabilitet jämfört med nämnda PB92-serinproteas, : i ! och att det kan framställas genom ett förfarande, i vilket 25 man odlar under lämpliga jäsningsförhällanden en värd- stamm, vilken är transformerad med en uttryckningsvektor, • · « vilken innehäller en DNA-sekvens, som kodar för ett mu- • · · ’·* * tantproteas och nämnda framställda proteas tas tillvara. • · · • · * • ·
2. Mutantproteas enligt patentkravet 1, kännetecknat där- • · « av, att det har den i patentkravet 1 framställda aminosy- :]*[: rasekvensen, under förutsättning, att i nämnda aminosyra- f.* . sekvens ätminstone en av följande positioner är muterade: * · G i positionen 116 till resten V, ”·’ 35 S i positionen 126 tili resten F, H, L, M, Q, R eller V. P i positionen 127 tili resten A, E, L, M, N, Q, S eller • f · v' 105481 59 S i positionen 128 tili resten A, G, K, P, T eller V, S i positionen 160 tili resten C, D, G eller N, N i positionen 212 tili resten D och M i positionen 216 tili resten Q eller S. 5
3. Mutantproteas enligt patentkravet 1 eller 2, kanne-tecknat därav, att det är en mutant av PB92-serinprotea-set: [M216S]; [M216Q]; [N212D]; [S160D]; [S160G, N212D]; 10 [S160D, M216Q]; [S160D, M216S]; [A166D, M169I]; [G116V, S126V, P127E, S128K]; [G116V, S126G, P127Q, S128I]; [G116V, S126L, P127N, S128V]; [G116V, S126L, P127Q, S128A3; [G116V, S126V, P127M]; [G116V, S126H, P127Y]; [G116V, S126R, P127S, S128P]; [G116V, S126F, P127Q]; 15 [G116V, S126F, P127L, S128T]; [S126M, P127A, S128G]; [S126M, P127A, S128G, S160D]; tai [G116V, S126N, P127S, S128A, S160D].
4. DNA-sekvens, kännetecknad därav, att den kodar för ett 20 mutantproteas enligt nägot av patentkraven 1-3.
5. Uttryckningsvektor, kännetecknad därav, att den inne-häller en DNA-sekvens enligt patentkravet 4. f t c
6. Bacillus-värdstam, kännetecknad därav, att den är transformerad med en uttryckningsvektor enligt patantkra-vet 5 och den är företrädesvis B. subtilis, B. licheni- • · · *;*/ formis eller B. amyloliquefaciens. m m · • · · • ·
7. Bacillus-värdstam enligt patentkravet 6, kännetecknad • · · därav, att den är en alkalofil Bacillus-stamm Bacillus nov. arten PB92 eller en mutant därav. ·«« * * « i « « • I I
8. Bacillus-vä rdstam enligt patentkravet 6, kännetecknad • · 35 därav, att den väsentligen är oförmögen att producera cellens externa proteolytiska enzymer före nämnda trans-: formation. • M 60 105481
9. Förfarande för framställning av ett mutantproteas en-ligt nägot av patentkraven 1-3, kännetecknat därav, att man odlar under lämpliga jäsningsförhällanden en värd-stam, vilken är transformerad med en uttryckningsvektor, 5 vilken innehäller en DNA-sekvens, vilken kodar för ett mutantproteas och nämnda framställda proteas tas tillvar.
10. En tvättmedelssaromansättning, kännetecknad därav, att den innehäller ett eller flera mutantproteaser enligt 10 nägot av patentkraven 1-3.
11. Användning av ett mutantproteas enligt nägot av patentkraven 1-3 i en tvättmedelssaromansättning.
12. Användning av ett mutantproteas enligt nägot av pa tentkraven 1-3 i en tvättprocess. « · « • « f • · · f • · · • Il « I · • I tl • • · · • · · · • · · * · · • ♦ · * • · • · · • ? 1 • · • · · • · • m »«· « · · • · • · • · · I r e « « · · f I I I « « « · IM • · • · • · ·
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP88200255 | 1988-02-11 | ||
EP88200255 | 1988-02-11 | ||
PCT/NL1989/000005 WO1989007642A1 (en) | 1988-02-11 | 1989-02-13 | Novel proteolytic enzymes and their use in detergents |
NL8900005 | 1989-02-13 |
Publications (2)
Publication Number | Publication Date |
---|---|
FI894799A0 FI894799A0 (fi) | 1989-10-10 |
FI105481B true FI105481B (sv) | 2000-08-31 |
Family
ID=8199752
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FI894799A FI105481B (sv) | 1988-02-11 | 1989-10-10 | Nya proteolytiska enzymer och deras användning i tvättmedel |
Country Status (21)
Country | Link |
---|---|
US (1) | US5336611A (sv) |
EP (3) | EP0328229B2 (sv) |
JP (1) | JP2849141B2 (sv) |
KR (1) | KR970005249B1 (sv) |
CN (1) | CN1056187C (sv) |
AT (1) | ATE250669T1 (sv) |
AU (1) | AU629814B2 (sv) |
BG (1) | BG60566B1 (sv) |
BR (1) | BR8905580A (sv) |
CA (1) | CA1340366C (sv) |
DE (2) | DE68929484T3 (sv) |
DK (1) | DK175813B1 (sv) |
ES (1) | ES2061929T5 (sv) |
FI (1) | FI105481B (sv) |
GR (1) | GR3021934T3 (sv) |
IE (2) | IE63248B1 (sv) |
LT (1) | LT3963B (sv) |
LV (1) | LV10652B (sv) |
PT (1) | PT89702B (sv) |
RU (1) | RU2069695C1 (sv) |
WO (1) | WO1989007642A1 (sv) |
Families Citing this family (124)
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1989
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