FI104539B - Förfarande för att producera en modifierad grupp B polysacharid av Neisseria meningitidis eller en modifierad E. coli K1-kapsulär polysackarid - Google Patents
Förfarande för att producera en modifierad grupp B polysacharid av Neisseria meningitidis eller en modifierad E. coli K1-kapsulär polysackarid Download PDFInfo
- Publication number
- FI104539B FI104539B FI922737A FI922737A FI104539B FI 104539 B FI104539 B FI 104539B FI 922737 A FI922737 A FI 922737A FI 922737 A FI922737 A FI 922737A FI 104539 B FI104539 B FI 104539B
- Authority
- FI
- Finland
- Prior art keywords
- gbmp
- polysaccharide
- group
- butanoyl
- protein
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
- C07K16/1203—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria
- C07K16/1228—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K16/1232—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia from Escherichia (G)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K39/025—Enterobacteriales, e.g. Enterobacter
- A61K39/0258—Escherichia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K39/095—Neisseria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
- C07K16/1203—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria
- C07K16/1217—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria from Neisseriaceae (F)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/60—Medicinal preparations containing antigens or antibodies characteristics by the carrier linked to the antigen
- A61K2039/6031—Proteins
- A61K2039/6037—Bacterial toxins, e.g. diphteria toxoid [DT], tetanus toxoid [TT]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Epidemiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Claims (13)
1. Förfarande för att producera en modifierad grupp B polysackarid av Neisseria 10 meningitidis eller en modifierad E. coli Kl-kapsulär polysackarid, kännetecknat av att N-acetylgruppema i sialinsyraresten i den nativa polysackariden ersätts med C4-Cg-acylgrupper och den modifierade polysackariden har en medelmolekylvikt inom omrädet 10 000 tili 50 000 Dalton.
2. Förfarande enligt patentkrav 1, kännetecknat av att C4-C8-acylgruppen är vald 15 ur den grupp som bestär av n-butanoyl, iso-butanoyl, n-pentanoyl, n-hexanoyl, n- heptanoyl och n-oktanoyl.
3. Förfarande enligt patentkrav 2, kännetecknat av att acylgruppen är vald ur den grupp som bestär av n-butanoyl, iso-butanoyl, n-pentanoyl och n-hexanoyl.
4. Förfarande enligt patentkrav 2, kännetecknat av att acylgruppen är n-butano- . 20 yl.
5. Förfarande enligt patentkrav 1, kännetecknat av att ca 27-100 % av N-acetyl-gruppema i sialinsyraresten har ersatts med C4-C8-acylgrupper.
6. Förfarande enligt patentkrav 5, kännetecknat av att ca 90-100 % av N-acetylgruppema i sialinsyraresten har ersatts med C4-C8-acylgrupper. ·> 25
7. Förfarande för att producera ett antigent konjugat, kännetecknat av att en : modifierad grupp B polysackarid av Neisseria meningitidis, vars N-acetylgrupp i sialinsyraresten är ersatt med en N-C4-C8-acylgrupp, konjugeras tili ett immunolo-giskt lämpligt protein. 104539
8. Förfarande enligt patentkrav 7, kännetecknat av att acylgruppen är vald ur den grupp som bestär av n-butanoyl, iso-butanoyl, pentanoyl, hexanoyl, heptanoyl och oktanoyl. r
9. Förfarande enligt patentkrav 7, kännetecknat av att acylgruppen är vald ur 5 den grupp som bestär av n-butanoyl, iso-butanoyl, pentanoyl och hexanoyl.
10. Förfarande enligt patentkrav 6, kännetecknat av att acylgruppen är n-butanoyl·
11. Förfarande enligt patentkrav 7, kännetecknat av att proteinet är valt ur den grupp som bestär av tetanustoxoid, difteritoxoid, ett korsreagerande material (CRM) 10 och en bakteriell proteinbärare.
12. Förfarande enligt patentkrav 7, kännetecknat av att proteinet och polysackari-den är kovalent kopplade genom en -CH2-NH-protein-koppling.
13. Förfarande enligt patentkrav 11, kännetecknat av att CRM är CRMi97. • ♦ « t ?
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US44819589A | 1989-12-14 | 1989-12-14 | |
US44819589 | 1989-12-14 | ||
PCT/CA1990/000437 WO1991008772A1 (en) | 1989-12-14 | 1990-12-13 | Improved meningococcal polysaccharide conjugate vaccine |
CA9000437 | 1990-12-13 |
Publications (2)
Publication Number | Publication Date |
---|---|
FI922737A0 FI922737A0 (fi) | 1992-06-12 |
FI104539B true FI104539B (sv) | 2000-02-29 |
Family
ID=23779371
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FI922737A FI104539B (sv) | 1989-12-14 | 1992-06-12 | Förfarande för att producera en modifierad grupp B polysacharid av Neisseria meningitidis eller en modifierad E. coli K1-kapsulär polysackarid |
FI991917A FI19991917A (sv) | 1989-12-14 | 1999-09-09 | Förbättrat meningokock-polysackaridkonjugatvaccin |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FI991917A FI19991917A (sv) | 1989-12-14 | 1999-09-09 | Förbättrat meningokock-polysackaridkonjugatvaccin |
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US (3) | US5576002A (sv) |
EP (1) | EP0504202B1 (sv) |
JP (1) | JP2637845B2 (sv) |
KR (1) | KR0158436B1 (sv) |
CN (4) | CN1049223C (sv) |
AR (1) | AR243934A1 (sv) |
AT (1) | ATE121947T1 (sv) |
AU (1) | AU641715B2 (sv) |
BR (1) | BR9007917A (sv) |
CA (1) | CA2071811C (sv) |
CZ (1) | CZ283530B6 (sv) |
DE (1) | DE69019164T2 (sv) |
DK (1) | DK0504202T3 (sv) |
ES (1) | ES2071288T3 (sv) |
FI (2) | FI104539B (sv) |
HR (1) | HRP920872A2 (sv) |
HU (2) | HU218146B (sv) |
IE (1) | IE68414B1 (sv) |
IL (1) | IL96676A (sv) |
IN (1) | IN171747B (sv) |
NO (2) | NO305275B1 (sv) |
NZ (1) | NZ236471A (sv) |
PH (1) | PH30305A (sv) |
PL (2) | PL166035B1 (sv) |
RO (1) | RO111416B1 (sv) |
RU (1) | RU2105568C1 (sv) |
SI (1) | SI20008B (sv) |
WO (1) | WO1991008772A1 (sv) |
YU (1) | YU24391A (sv) |
ZA (1) | ZA9010065B (sv) |
Families Citing this family (47)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5648241A (en) * | 1989-09-15 | 1997-07-15 | The General Hospital Corporation | Conjugate vaccine against group B streptococcus |
DK0504202T3 (da) * | 1989-12-14 | 1995-10-02 | Ca Nat Research Council | Forbedret meningokok-polysaccharid-konjugat vaccine |
DE69330464T2 (de) * | 1992-09-24 | 2001-11-08 | Brigham And Women's Hospital, Boston | Gruppe b streptococcus typ ii und typ v polysaccharid-protein konjugate als impfstoffe |
US6153406A (en) * | 1993-07-23 | 2000-11-28 | North American Vaccine, Inc. | Method for the high level expression, purification and refolding of the outer membrane protein P2 from Haemophilus influenzae type B |
US5439808A (en) * | 1993-07-23 | 1995-08-08 | North American Vaccine, Inc. | Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis |
US5679654A (en) * | 1994-09-02 | 1997-10-21 | Brigham & Women's Hospital, Inc. | Capsular polysaccharide immunomodulator |
US5700787A (en) * | 1994-09-02 | 1997-12-23 | Brigham & Women's Hospital, Inc. | Capsular polysaccharide immunomodulator |
US5747287A (en) * | 1995-04-28 | 1998-05-05 | North American Vaccine, Inc. | Method for the high level expression, purification and refolding of the outer membrane group B porin proteins from Neisseria meningitidis |
US5811102A (en) * | 1995-06-07 | 1998-09-22 | National Research Council Of Canada | Modified meningococcal polysaccharide conjugate vaccines |
ES2308962T3 (es) * | 1995-06-07 | 2008-12-16 | Glaxosmithkline Biologicals S.A. | Vacunas que comprenden un conjugado de antigeno polisacarido-proteina transportadora y una proteina transportadora libre. |
US6284884B1 (en) | 1995-06-07 | 2001-09-04 | North American Vaccine, Inc. | Antigenic group B streptococcus type II and type III polysaccharide fragments having a 2,5-anhydro-D-mannose terminal structure and conjugate vaccine thereof |
ZA97248B (en) * | 1996-01-18 | 1997-07-18 | Rohm & Haas | Method for identifying and quantifying polymers utilizing immunoassay techniques |
ATE252602T1 (de) * | 1996-08-27 | 2003-11-15 | Chiron Corp | Meningokokkus b-epitop ausbildende monoklonale antikoerper und deren verwendung zur herstellung von impfstoffzusammenstellungen |
DE69708318T3 (de) * | 1996-08-27 | 2006-11-16 | Novartis Vaccines and Diagnostics, Inc., Emeryville | Neisseria meningitidis serogruppe b glykokonjugate und verfahren zu deren verwendung |
US6426074B1 (en) | 1997-03-19 | 2002-07-30 | The Brigham And Women's Hospital Inc. | Group B Streptococcus vaccine |
EP0994723A1 (en) | 1997-06-24 | 2000-04-26 | Chiron Corporation | Methods of immunizing adults using anti-meningococcal vaccine compositions |
EP1007546B1 (en) * | 1997-08-27 | 2009-01-21 | Novartis Vaccines and Diagnostics, Inc. | Molecular mimetics of meningococcal b epitopes |
ES2346022T3 (es) | 1997-12-23 | 2010-10-07 | Baxter Healthcare S.A. | Procedimiento para la extraccion y el aislamiento de polisacaridos capsulares bacterianos para su uso como vacunas o ligandos a proteinas como vacunas de conjugados. |
JP2004505885A (ja) * | 1998-08-19 | 2004-02-26 | ノース アメリカン ワクチン, インコーポレイテッド | N−アクリロイル化ポリサッカリドを用いて産生されたワクチンとして有用な免疫原性β−プロピオンアミド連結ポリサッカリド−タンパク質結合体 |
US6436653B1 (en) | 1998-12-15 | 2002-08-20 | Exiqon A/S | Method for introduction of reporter groups into bacterial lipopolysaccharide-derived carbohydrates and the subsequent coupling of such derivatives onto solid surfaces |
US7083777B1 (en) * | 1999-04-02 | 2006-08-01 | The Brigham And Women's Hospital, Inc. | Immunomodulating polymers |
US6518037B2 (en) * | 1999-11-12 | 2003-02-11 | University Of Iowa Research Foundation | Two-component system that controls bacterial membrane synthesis |
GB9928196D0 (en) * | 1999-11-29 | 2000-01-26 | Chiron Spa | Combinations of B, C and other antigens |
SV2003000753A (es) | 2000-12-05 | 2003-06-16 | Brigham & Womens Hospital | Uso de polisacaridos zwitterionicos para la especifica modulacion del progreso inmunologico |
ATE455793T1 (de) | 2001-04-17 | 2010-02-15 | Novartis Vaccines & Diagnostic | Monoklonale antikörper gegen molekulare mimetika von meningokokken-b-epitopen |
GB0115176D0 (en) * | 2001-06-20 | 2001-08-15 | Chiron Spa | Capular polysaccharide solubilisation and combination vaccines |
GB0121591D0 (en) * | 2001-09-06 | 2001-10-24 | Chiron Spa | Hybrid and tandem expression of neisserial proteins |
AR045702A1 (es) | 2001-10-03 | 2005-11-09 | Chiron Corp | Composiciones de adyuvantes. |
WO2003080678A1 (en) * | 2002-03-26 | 2003-10-02 | Chiron Srl | Modified saccharides having improved stability in water |
EP1638581A2 (en) * | 2003-03-31 | 2006-03-29 | The Brigham And Women's Hospital, Inc. | Zwitterionic immunomodulators for the treatment of asthma and allergy |
US7731967B2 (en) | 2003-04-30 | 2010-06-08 | Novartis Vaccines And Diagnostics, Inc. | Compositions for inducing immune responses |
KR101113726B1 (ko) * | 2003-08-12 | 2012-02-27 | 리폭센 테크놀로지즈 리미티드 | 단백질 유도 및 컨쥬게이션용 시알산 유도체 |
US8148335B2 (en) | 2004-06-23 | 2012-04-03 | Children's Hospital & Research Center Oakland | De-N-acetyl sialic acid antigens, antibodies thereto, and methods of use in cancer therapy |
JP2008509886A (ja) | 2004-06-23 | 2008-04-03 | チルドレンズ ホスピタル アンド リサーチ センター アット オークランド | 多糖誘導体および免疫応答の誘導における用途 |
GB0428394D0 (en) * | 2004-12-24 | 2005-02-02 | Chiron Srl | Saccharide conjugate vaccines |
US9931397B2 (en) | 2005-06-27 | 2018-04-03 | Glaxosmithkline Biologicals S.A. | Immunogenic composition |
US8206726B2 (en) | 2006-02-06 | 2012-06-26 | The Brigham And Women's Hospital, Inc. | Zwitterionic polysaccharides for promotion of immune system maturation and health |
GB0611914D0 (en) | 2006-06-15 | 2006-07-26 | Teti Giuseppe | Peptides that mimic non-human cross-reactive protective epitopes of the group Bmeningococcal capsulsar polysaccharide |
EP1872791A1 (en) | 2006-06-30 | 2008-01-02 | Institut Pasteur | Use of bacterial polysaccharides for biofilm inhibition |
WO2008157590A1 (en) * | 2007-06-20 | 2008-12-24 | Baxter International Inc. | Modified polysaccharides for conjugate vaccines |
ES2664753T3 (es) | 2007-12-07 | 2018-04-23 | Glaxosmithkline Biologicals Sa | Composiciones de inducción de respuestas inmunes |
EP2387417B1 (en) | 2009-01-16 | 2016-05-11 | University of Maryland, Baltimore | Broad spectrum vaccine against non-typhoidal salmonella |
JP6273200B2 (ja) | 2011-07-12 | 2018-01-31 | ザ・ブリガーム・アンド・ウーメンズ・ホスピタル・インコーポレーテッド | 脂質含有psa組成物、その単離の方法および使用の方法 |
EP2752403A1 (de) * | 2013-01-08 | 2014-07-09 | Sika Technology AG | Amin für emissionsarme Epoxidharz-Produkte |
EP3337321A4 (en) | 2015-08-19 | 2019-07-17 | President and Fellows of Harvard College | LIPIDED PSA COMPOSITIONS AND METHOD |
EP3484441A4 (en) | 2016-07-15 | 2020-03-18 | President and Fellows of Harvard College | GLYCOLIPID COMPOSITIONS AND METHODS OF USE |
WO2018142280A2 (en) * | 2017-01-31 | 2018-08-09 | Pfizer Inc. | Neisseria meningitidis compositions and methods thereof |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4057685A (en) * | 1972-02-02 | 1977-11-08 | Abbott Laboratories | Chemically modified endotoxin immunizing agent |
US4356170A (en) * | 1981-05-27 | 1982-10-26 | Canadian Patents & Development Ltd. | Immunogenic polysaccharide-protein conjugates |
US4673574A (en) * | 1981-08-31 | 1987-06-16 | Anderson Porter W | Immunogenic conjugates |
US4644059A (en) * | 1982-07-06 | 1987-02-17 | Connaught Laboratories, Inc. | Haemophilus influenzae B polysaccharide-diptheria toxoid conjugate vaccine |
US4496538A (en) * | 1982-07-06 | 1985-01-29 | Connaught Laboratories, Inc. | Haemophilus influenzae b polysaccharide-diphtheria toxoid conjugate vaccine |
US4619828A (en) * | 1982-07-06 | 1986-10-28 | Connaught Laboratories, Inc. | Polysaccharide exotoxoid conjugate vaccines |
US4695624A (en) * | 1984-05-10 | 1987-09-22 | Merck & Co., Inc. | Covalently-modified polyanionic bacterial polysaccharides, stable covalent conjugates of such polysaccharides and immunogenic proteins with bigeneric spacers, and methods of preparing such polysaccharides and conjugates and of confirming covalency |
US4727136A (en) * | 1985-10-01 | 1988-02-23 | Canadian Patents And Development Ltd. | Modified meningococcal group B polysaccharide for conjugate vaccine |
DK0504202T3 (da) * | 1989-12-14 | 1995-10-02 | Ca Nat Research Council | Forbedret meningokok-polysaccharid-konjugat vaccine |
-
1990
- 1990-12-13 DK DK91900142.0T patent/DK0504202T3/da active
- 1990-12-13 RO RO92-0789A patent/RO111416B1/ro unknown
- 1990-12-13 AU AU68987/91A patent/AU641715B2/en not_active Ceased
- 1990-12-13 HU HU9201966A patent/HU218146B/hu unknown
- 1990-12-13 ES ES91900142T patent/ES2071288T3/es not_active Expired - Lifetime
- 1990-12-13 AT AT91900142T patent/ATE121947T1/de not_active IP Right Cessation
- 1990-12-13 EP EP91900142A patent/EP0504202B1/en not_active Expired - Lifetime
- 1990-12-13 KR KR1019920701403A patent/KR0158436B1/ko not_active IP Right Cessation
- 1990-12-13 DE DE69019164T patent/DE69019164T2/de not_active Expired - Fee Related
- 1990-12-13 CA CA002071811A patent/CA2071811C/en not_active Expired - Fee Related
- 1990-12-13 RU SU5052391A patent/RU2105568C1/ru active
- 1990-12-13 JP JP3500908A patent/JP2637845B2/ja not_active Expired - Fee Related
- 1990-12-13 BR BR909007917A patent/BR9007917A/pt not_active Application Discontinuation
- 1990-12-13 HU HU9201966A patent/HU9201966D0/hu unknown
- 1990-12-13 WO PCT/CA1990/000437 patent/WO1991008772A1/en active IP Right Grant
- 1990-12-14 IE IE451390A patent/IE68414B1/en not_active IP Right Cessation
- 1990-12-14 SI SI9110243A patent/SI20008B/sl not_active IP Right Cessation
- 1990-12-14 AR AR90318627A patent/AR243934A1/es active
- 1990-12-14 ZA ZA9010065A patent/ZA9010065B/xx unknown
- 1990-12-14 IL IL9667690A patent/IL96676A/en not_active IP Right Cessation
- 1990-12-14 IN IN1033/CAL/90A patent/IN171747B/en unknown
- 1990-12-14 NZ NZ236471A patent/NZ236471A/en unknown
- 1990-12-14 CZ CS906284A patent/CZ283530B6/cs unknown
- 1990-12-14 PL PL90288271A patent/PL166035B1/pl not_active IP Right Cessation
- 1990-12-14 PL PL90302476A patent/PL166659B1/pl not_active IP Right Cessation
- 1990-12-14 CN CN90110444A patent/CN1049223C/zh not_active Expired - Fee Related
- 1990-12-14 YU YU24391A patent/YU24391A/sh unknown
- 1990-12-14 PH PH41724A patent/PH30305A/en unknown
-
1992
- 1992-06-12 FI FI922737A patent/FI104539B/sv active
- 1992-06-12 NO NO922316A patent/NO305275B1/no unknown
- 1992-10-02 HR HRP-243/91A patent/HRP920872A2/hr not_active Application Discontinuation
-
1993
- 1993-11-22 CN CN93119467A patent/CN1036504C/zh not_active Expired - Fee Related
- 1993-11-22 CN CN93114764A patent/CN1072506C/zh not_active Expired - Fee Related
- 1993-11-22 CN CN93114763A patent/CN1036522C/zh not_active Expired - Fee Related
-
1994
- 1994-05-05 US US08/238,600 patent/US5576002A/en not_active Expired - Lifetime
-
1995
- 1995-06-07 US US08/485,623 patent/US5683699A/en not_active Expired - Lifetime
-
1997
- 1997-07-17 NO NO973311A patent/NO307835B1/no unknown
- 1997-10-17 US US08/953,771 patent/US5902586A/en not_active Expired - Fee Related
-
1999
- 1999-09-09 FI FI991917A patent/FI19991917A/sv unknown
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