ES2807530T3 - Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona - Google Patents

Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona Download PDF

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Publication number
ES2807530T3
ES2807530T3 ES17151869T ES17151869T ES2807530T3 ES 2807530 T3 ES2807530 T3 ES 2807530T3 ES 17151869 T ES17151869 T ES 17151869T ES 17151869 T ES17151869 T ES 17151869T ES 2807530 T3 ES2807530 T3 ES 2807530T3
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Spain
Prior art keywords
gdh
mutant
glucose
amino acid
improve
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Active
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ES17151869T
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English (en)
Spanish (es)
Inventor
Mara Boenitz-Dulat
Daniela Beck
Peter Kratzsch
Rainer Schmuck
Der Eltz Herbert Von
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
ES17151869T 2006-04-13 2007-04-11 Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona Active ES2807530T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP06007779 2006-04-13

Publications (1)

Publication Number Publication Date
ES2807530T3 true ES2807530T3 (es) 2021-02-23

Family

ID=36579921

Family Applications (2)

Application Number Title Priority Date Filing Date
ES17151869T Active ES2807530T3 (es) 2006-04-13 2007-04-11 Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona
ES07724148.7T Active ES2624805T3 (es) 2006-04-13 2007-04-11 Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES07724148.7T Active ES2624805T3 (es) 2006-04-13 2007-04-11 Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona

Country Status (14)

Country Link
US (1) US7732179B2 (https=)
EP (2) EP2010649B1 (https=)
JP (3) JP5683809B2 (https=)
KR (1) KR101168623B1 (https=)
CN (1) CN101421396B (https=)
AU (1) AU2007237478B2 (https=)
BR (1) BRPI0710131B1 (https=)
CA (1) CA2649135C (https=)
ES (2) ES2807530T3 (https=)
HU (2) HUE049911T2 (https=)
MX (1) MX2008013001A (https=)
PL (2) PL2010649T3 (https=)
PT (2) PT2010649T (https=)
WO (1) WO2007118647A1 (https=)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5753720B2 (ja) * 2010-04-22 2015-07-22 アークレイ株式会社 バイオセンサ
ES2568464T3 (es) 2010-07-23 2016-04-29 F. Hoffmann-La Roche Ag Composiciones que contienen tampón zwiteriónico y usos en dispositivos y métodos electroanalíticos
AU2011355209B2 (en) 2010-12-20 2015-09-17 Lek Pharmaceuticals D.D. Enzymatic synthesis of active pharmaceutical ingredient and intermediates thereof
EP2465936A1 (en) 2010-12-20 2012-06-20 LEK Pharmaceuticals d.d. Enzymatic synthesis of statins and intermediates thereof
EP3901624B1 (en) 2010-12-22 2024-01-31 Roche Diabetes Care GmbH Methods to compensate for sources of error during electrochemical testing
CN102559624B (zh) * 2012-03-05 2013-10-16 浙江德清汇宁生物科技有限公司 一种吡咯喹啉醌依赖型葡萄糖脱氢酶的热稳定性突变体及其高通量筛选方法
KR101728597B1 (ko) 2012-04-19 2017-04-19 에프. 호프만-라 로슈 아게 혈액 내 분석물 농도를 결정하는 방법 및 디바이스
EP2811299A1 (en) 2013-06-07 2014-12-10 Roche Diagniostics GmbH Test element for detecting at least one analyte in a body fluid
CN108026560A (zh) 2015-09-25 2018-05-11 豪夫迈·罗氏有限公司 在低共熔溶剂中使用分选酶的转酰胺反应
CN108138204B (zh) 2015-09-25 2021-12-31 豪夫迈·罗氏有限公司 使用分选酶a生产硫酯的方法
CN105331590B (zh) * 2015-10-29 2020-02-28 英科隆生物技术（杭州）有限公司 葡萄糖特异性改善的可溶性吡咯喹啉醌依赖性葡萄糖脱氢酶突变体、聚核苷酸及其应用
CN105331591B (zh) * 2015-10-29 2020-02-28 英科隆生物技术（杭州）有限公司 PQQ-sGDH突变体、聚核苷酸及葡萄糖检测装置
CN108350439A (zh) 2015-12-21 2018-07-31 豪夫迈·罗氏有限公司 来自粪产碱菌的突变体3-羟基丁酸脱氢酶及其相关方法和用途
EP3414325B1 (en) 2016-02-09 2020-01-29 Roche Diabetes Care GmbH Mutant 3-hydroxybutyrate dehydrogenase from rhodobacter sphaeroides as well as methods and uses involving the same
CA3035874C (en) 2016-10-05 2025-09-09 F. Hoffmann-La Roche Ag DETECTION REAGENTS AND ELECTRODE ARRANGEMENTS FOR MULTI-ANALYTICAL DIAGNOSTIC TEST ELEMENTS, AS WELL AS THEIR METHODS OF USE
CN115820608B (zh) * 2022-08-31 2024-06-04 中国海洋大学 λ-卡拉胶酶突变体OUC-CglA-DPQQ及其应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3716957A1 (de) 1987-05-20 1988-12-01 Boehringer Mannheim Gmbh Expressionsvektor zur regulierbaren expression von fremdgenen in prokaryonten
DE4311464A1 (de) 1993-04-08 1994-10-13 Boehringer Mannheim Gmbh Verfahren zur kolorimetrischen Bestimmung eines Analyten mit einer PQQ-abhängigen Dehydrogenase
DE19639169A1 (de) 1996-09-24 1998-04-02 Boehringer Mannheim Gmbh Redoxaktive Verbindungen und deren Anwendung
JPH10243786A (ja) 1997-03-03 1998-09-14 Koji Hayade 改変型グルコース脱水素酵素
US5997817A (en) 1997-12-05 1999-12-07 Roche Diagnostics Corporation Electrochemical biosensor test strip
JPH11243949A (ja) 1998-03-03 1999-09-14 Toyobo Co Ltd Ｐｑｑを補欠分子族とするグルコースデヒドロゲナーゼおよびその製造方法
KR20010103017A (ko) * 1999-02-19 2001-11-17 플레믹 크리스티안 글루코스 데히드로게나제 융합 단백질 및 발현계에서의그의 용도
JP2000350588A (ja) * 1999-04-08 2000-12-19 Koji Hayade グルコース脱水素酵素
TWI224136B (en) 1999-04-30 2004-11-21 Koji Sode Glucose dehydrogenase
AU1596602A (en) * 2000-10-27 2002-05-06 Peter Kratzsch Variants of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase
JP2004344145A (ja) * 2002-05-27 2004-12-09 Toyobo Co Ltd 基質特異性または安定性に優れたピロロキノリンキノン（ｐｑｑ）依存性グルコースデヒドロゲナーゼ改変体
JP2004173538A (ja) 2002-11-25 2004-06-24 Amano Enzyme Inc ピロロキノリンキノン依存性グルコース脱水素酵素
AU2005263591B2 (en) * 2004-07-20 2008-08-14 F. Hoffmann-La Roche Ag Genetically engineered pyrroloquinoline quinone dependent glucose dehydrogenase comprising an amino acid insertion

Also Published As

Publication number Publication date
CA2649135C (en) 2013-11-19
JP2009533033A (ja) 2009-09-17
PL2010649T3 (pl) 2017-08-31
AU2007237478A1 (en) 2007-10-25
HK1131633A1 (zh) 2010-01-29
PT2010649T (pt) 2017-05-03
PL3246402T3 (pl) 2020-10-19
EP3246402B1 (en) 2020-05-20
EP3246402A1 (en) 2017-11-22
MX2008013001A (es) 2008-10-17
ES2624805T3 (es) 2017-07-17
KR101168623B1 (ko) 2012-08-03
JP5683809B2 (ja) 2015-03-11
CN101421396A (zh) 2009-04-29
US7732179B2 (en) 2010-06-08
CA2649135A1 (en) 2007-10-25
AU2007237478B2 (en) 2011-05-19
WO2007118647A1 (en) 2007-10-25
HUE032804T2 (en) 2017-11-28
CN101421396B (zh) 2016-01-20
JP5937547B2 (ja) 2016-06-22
BRPI0710131B1 (pt) 2020-03-10
HUE049911T2 (hu) 2020-11-30
JP2016119904A (ja) 2016-07-07
KR20080106349A (ko) 2008-12-04
BRPI0710131A2 (pt) 2011-08-02
EP2010649A1 (en) 2009-01-07
JP2013198497A (ja) 2013-10-03
PT3246402T (pt) 2020-07-14
EP2010649B1 (en) 2017-03-08
US20090148874A1 (en) 2009-06-11
JP6185617B2 (ja) 2017-08-23

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ES2807530T3 (es) Mutantes mejorados de glucosa deshidrogenasa soluble dependiente de pirroloquinolina quinona
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NO340499B1 (no) Variant av den løselige formen av pyrrolokinolinkinonavhengig glukosedehydrogenase som omfatter én aminosyre insersjon og minst én aminosyre substitusjon, et isolert polynukleotid som koder for varianten, en ekspresjonsvektor som omfatter det isolert polynukleotidet, en vertscelle som omfatter ekspresjonsvektoren, en fremgangsmåte for fremstilling av s-GDH-varianter som omfatter dyrking av vertscellen, en fremgangsmåte for fremstilling av s-GDH-varianter i et cellefritt peptidsyntesesystem, en fremgangsmåte for påvisning, bestemmelse eller måling av glukose i en prøve ved å anvende en variant, samt en innretning for påvisningen eller målingen av glukose i en prøve.
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