CN101421396B - 吡咯并喹啉醌依赖性可溶性葡萄糖脱氢酶的改良突变体 - Google Patents

吡咯并喹啉醌依赖性可溶性葡萄糖脱氢酶的改良突变体 Download PDF

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CN101421396B
CN101421396B CN200780013259.8A CN200780013259A CN101421396B CN 101421396 B CN101421396 B CN 101421396B CN 200780013259 A CN200780013259 A CN 200780013259A CN 101421396 B CN101421396 B CN 101421396B
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mutant
gdh
glucose
amino acid
mutants
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CN101421396A (zh
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M·博尼茨-多莱特
D·贝克
P·克拉茨什
R·施穆克
H·冯德埃尔茨
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)

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  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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CN200780013259.8A 2006-04-13 2007-04-11 吡咯并喹啉醌依赖性可溶性葡萄糖脱氢酶的改良突变体 Active CN101421396B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP06007779.9 2006-04-13
EP06007779 2006-04-13
PCT/EP2007/003207 WO2007118647A1 (en) 2006-04-13 2007-04-11 Improved mutants of pyrroloquinoline quinone dependent soluble glucose dehydrogenase

Publications (2)

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CN101421396A CN101421396A (zh) 2009-04-29
CN101421396B true CN101421396B (zh) 2016-01-20

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Country Link
US (1) US7732179B2 (https=)
EP (2) EP2010649B1 (https=)
JP (3) JP5683809B2 (https=)
KR (1) KR101168623B1 (https=)
CN (1) CN101421396B (https=)
AU (1) AU2007237478B2 (https=)
BR (1) BRPI0710131B1 (https=)
CA (1) CA2649135C (https=)
ES (2) ES2807530T3 (https=)
HU (2) HUE049911T2 (https=)
MX (1) MX2008013001A (https=)
PL (2) PL2010649T3 (https=)
PT (2) PT2010649T (https=)
WO (1) WO2007118647A1 (https=)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5753720B2 (ja) * 2010-04-22 2015-07-22 アークレイ株式会社 バイオセンサ
ES2568464T3 (es) 2010-07-23 2016-04-29 F. Hoffmann-La Roche Ag Composiciones que contienen tampón zwiteriónico y usos en dispositivos y métodos electroanalíticos
AU2011355209B2 (en) 2010-12-20 2015-09-17 Lek Pharmaceuticals D.D. Enzymatic synthesis of active pharmaceutical ingredient and intermediates thereof
EP2465936A1 (en) 2010-12-20 2012-06-20 LEK Pharmaceuticals d.d. Enzymatic synthesis of statins and intermediates thereof
EP3901624B1 (en) 2010-12-22 2024-01-31 Roche Diabetes Care GmbH Methods to compensate for sources of error during electrochemical testing
CN102559624B (zh) * 2012-03-05 2013-10-16 浙江德清汇宁生物科技有限公司 一种吡咯喹啉醌依赖型葡萄糖脱氢酶的热稳定性突变体及其高通量筛选方法
KR101728597B1 (ko) 2012-04-19 2017-04-19 에프. 호프만-라 로슈 아게 혈액 내 분석물 농도를 결정하는 방법 및 디바이스
EP2811299A1 (en) 2013-06-07 2014-12-10 Roche Diagniostics GmbH Test element for detecting at least one analyte in a body fluid
CN108026560A (zh) 2015-09-25 2018-05-11 豪夫迈·罗氏有限公司 在低共熔溶剂中使用分选酶的转酰胺反应
CN108138204B (zh) 2015-09-25 2021-12-31 豪夫迈·罗氏有限公司 使用分选酶a生产硫酯的方法
CN105331590B (zh) * 2015-10-29 2020-02-28 英科隆生物技术（杭州）有限公司 葡萄糖特异性改善的可溶性吡咯喹啉醌依赖性葡萄糖脱氢酶突变体、聚核苷酸及其应用
CN105331591B (zh) * 2015-10-29 2020-02-28 英科隆生物技术（杭州）有限公司 PQQ-sGDH突变体、聚核苷酸及葡萄糖检测装置
CN108350439A (zh) 2015-12-21 2018-07-31 豪夫迈·罗氏有限公司 来自粪产碱菌的突变体3-羟基丁酸脱氢酶及其相关方法和用途
EP3414325B1 (en) 2016-02-09 2020-01-29 Roche Diabetes Care GmbH Mutant 3-hydroxybutyrate dehydrogenase from rhodobacter sphaeroides as well as methods and uses involving the same
CA3035874C (en) 2016-10-05 2025-09-09 F. Hoffmann-La Roche Ag DETECTION REAGENTS AND ELECTRODE ARRANGEMENTS FOR MULTI-ANALYTICAL DIAGNOSTIC TEST ELEMENTS, AS WELL AS THEIR METHODS OF USE
CN115820608B (zh) * 2022-08-31 2024-06-04 中国海洋大学 λ-卡拉胶酶突变体OUC-CglA-DPQQ及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1340104A (zh) * 1999-02-19 2002-03-13 默克专利股份有限公司 葡萄糖脱氢酶融合蛋白及其在表达系统中的用途
CN1350579A (zh) * 1999-04-08 2002-05-22 早出广司 葡萄糖脱氢酶
US20030104595A1 (en) * 2000-10-27 2003-06-05 Peter Kratzch Forms of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3716957A1 (de) 1987-05-20 1988-12-01 Boehringer Mannheim Gmbh Expressionsvektor zur regulierbaren expression von fremdgenen in prokaryonten
DE4311464A1 (de) 1993-04-08 1994-10-13 Boehringer Mannheim Gmbh Verfahren zur kolorimetrischen Bestimmung eines Analyten mit einer PQQ-abhängigen Dehydrogenase
DE19639169A1 (de) 1996-09-24 1998-04-02 Boehringer Mannheim Gmbh Redoxaktive Verbindungen und deren Anwendung
JPH10243786A (ja) 1997-03-03 1998-09-14 Koji Hayade 改変型グルコース脱水素酵素
US5997817A (en) 1997-12-05 1999-12-07 Roche Diagnostics Corporation Electrochemical biosensor test strip
JPH11243949A (ja) 1998-03-03 1999-09-14 Toyobo Co Ltd Ｐｑｑを補欠分子族とするグルコースデヒドロゲナーゼおよびその製造方法
TWI224136B (en) 1999-04-30 2004-11-21 Koji Sode Glucose dehydrogenase
JP2004344145A (ja) * 2002-05-27 2004-12-09 Toyobo Co Ltd 基質特異性または安定性に優れたピロロキノリンキノン（ｐｑｑ）依存性グルコースデヒドロゲナーゼ改変体
JP2004173538A (ja) 2002-11-25 2004-06-24 Amano Enzyme Inc ピロロキノリンキノン依存性グルコース脱水素酵素
AU2005263591B2 (en) * 2004-07-20 2008-08-14 F. Hoffmann-La Roche Ag Genetically engineered pyrroloquinoline quinone dependent glucose dehydrogenase comprising an amino acid insertion

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1340104A (zh) * 1999-02-19 2002-03-13 默克专利股份有限公司 葡萄糖脱氢酶融合蛋白及其在表达系统中的用途
CN1350579A (zh) * 1999-04-08 2002-05-22 早出广司 葡萄糖脱氢酶
US20030104595A1 (en) * 2000-10-27 2003-06-05 Peter Kratzch Forms of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase

Also Published As

Publication number Publication date
CA2649135C (en) 2013-11-19
JP2009533033A (ja) 2009-09-17
PL2010649T3 (pl) 2017-08-31
AU2007237478A1 (en) 2007-10-25
HK1131633A1 (zh) 2010-01-29
PT2010649T (pt) 2017-05-03
PL3246402T3 (pl) 2020-10-19
EP3246402B1 (en) 2020-05-20
EP3246402A1 (en) 2017-11-22
MX2008013001A (es) 2008-10-17
ES2624805T3 (es) 2017-07-17
KR101168623B1 (ko) 2012-08-03
JP5683809B2 (ja) 2015-03-11
CN101421396A (zh) 2009-04-29
US7732179B2 (en) 2010-06-08
CA2649135A1 (en) 2007-10-25
AU2007237478B2 (en) 2011-05-19
WO2007118647A1 (en) 2007-10-25
HUE032804T2 (en) 2017-11-28
JP5937547B2 (ja) 2016-06-22
BRPI0710131B1 (pt) 2020-03-10
HUE049911T2 (hu) 2020-11-30
JP2016119904A (ja) 2016-07-07
KR20080106349A (ko) 2008-12-04
BRPI0710131A2 (pt) 2011-08-02
EP2010649A1 (en) 2009-01-07
JP2013198497A (ja) 2013-10-03
PT3246402T (pt) 2020-07-14
ES2807530T3 (es) 2021-02-23
EP2010649B1 (en) 2017-03-08
US20090148874A1 (en) 2009-06-11
JP6185617B2 (ja) 2017-08-23

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