ES2629502T3 - Métodos y composiciones relacionados con exosomas de células madre mesenquimales - Google Patents
Métodos y composiciones relacionados con exosomas de células madre mesenquimales Download PDFInfo
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- ES2629502T3 ES2629502T3 ES12709484.5T ES12709484T ES2629502T3 ES 2629502 T3 ES2629502 T3 ES 2629502T3 ES 12709484 T ES12709484 T ES 12709484T ES 2629502 T3 ES2629502 T3 ES 2629502T3
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| CN103648509B (zh) * | 2011-03-11 | 2019-02-22 | 儿童医学中心公司 | 与间充质干细胞外来体相关的方法和组合物 |
| ITRM20110403A1 (it) * | 2011-07-28 | 2013-01-29 | Ospedale Pediatrico Bambino Gesu | Microvescicole isolate da cellule mesenchimali come agenti immunosoppressori. |
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| JP6433896B2 (ja) | 2012-08-13 | 2018-12-05 | シーダーズ−サイナイ・メディカル・センターＣｅｄａｒｓ−Ｓｉｎａｉ Ｍｅｄｉｃａｌ Ｃｅｎｔｅｒ | 組織再生のためのエキソソームおよびマイクロリボ核酸 |
| CN103865873B (zh) * | 2012-12-12 | 2017-04-05 | 中国医学科学院基础医学研究所 | 亚全能干细胞分泌的外来体及其应用 |
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| JP6343671B2 (ja) * | 2013-12-12 | 2018-06-13 | サムスン ライフ パブリック ウェルフェア ファウンデーション | トロンビンを利用した幹細胞由来のエキソソームの生成促進方法 |
| US11078462B2 (en) | 2014-02-18 | 2021-08-03 | ReCyte Therapeutics, Inc. | Perivascular stromal cells from primate pluripotent stem cells |
| KR101661847B1 (ko) | 2014-03-18 | 2016-09-30 | 사회복지법인 삼성생명공익재단 | 줄기세포 유래 엑소좀을 유효성분으로 포함하는 뇌 염증성 질환의 치료용 조성물 |
| CN106714781B (zh) | 2014-05-18 | 2021-08-10 | 儿童医学中心公司 | 涉及外排体的方法和组合物 |
| WO2015191545A1 (en) * | 2014-06-09 | 2015-12-17 | University Of Washington | Methods of protection against ischemia reperfusion injury |
| RU2715866C2 (ru) * | 2014-06-30 | 2020-03-03 | Тихеникс С.А.У. | Мезенхимальные стромальные клетки для лечения синдрома сепсиса |
| US10240127B2 (en) | 2014-07-03 | 2019-03-26 | ReCyte Therapeutics, Inc. | Exosomes from clonal progenitor cells |
| EP3209768A1 (en) * | 2014-07-25 | 2017-08-30 | Recellerate Inc. | Methods of treating exercise-induced pulmonary hemorrhage |
| CA2962444C (en) | 2014-10-03 | 2023-09-05 | Cedars-Sinai Medical Center | Cardiosphere-derived cells and exosomes secreted by such cells in the treatment of muscular dystrophy |
| US20170304368A1 (en) * | 2014-10-06 | 2017-10-26 | Cedars-Sinai Medical Center | Polarization of macrophages to a healing phenotype by cardiosphere-derived cells and by the exosomes secreted by such cells |
| WO2016072821A1 (ko) * | 2014-11-07 | 2016-05-12 | 한양대학교 에리카산학협력단 | 줄기세포 유래 엑소좀을 함유하는 지방세포 분화유도, 지방조직 재생, 피부 미백 또는 주름개선용 조성물 |
| CN118161529A (zh) * | 2014-11-07 | 2024-06-11 | 胞外体干细胞株式会社 | 用于成脂分化诱导、脂肪组织再生、皮肤美白或改善皱纹的包含干细胞来源外泌体的组合物 |
| US20160160181A1 (en) * | 2014-12-03 | 2016-06-09 | Capricor Thera[eitocs, Inc. | Processes for producing exosomes in reduced oxygen culture conditions |
| CN104560877B (zh) * | 2014-12-18 | 2017-07-28 | 福州市传染病医院 | 一种快速分离细胞外吐小体的方法 |
| CA2972226A1 (en) * | 2014-12-24 | 2016-06-30 | Ube Industries, Ltd. | Cell culture supernatant fluid derived from lung tissue |
| US10342830B2 (en) * | 2015-01-05 | 2019-07-09 | Gary M. Petrucci | Methods and materials for treating lung disorders |
| US9687511B2 (en) | 2015-03-06 | 2017-06-27 | Vivex Biomedical, Inc. | Acellular biologic composition and method of manufacture |
| US11160904B2 (en) * | 2017-05-09 | 2021-11-02 | Vivex Biologies Group, Inc. | Biological composition in a protectant shroud and methods |
| US20180325830A1 (en) | 2017-05-09 | 2018-11-15 | Vivex Biomedical, Inc. | Coated biological composition |
| KR101566450B1 (ko) * | 2015-04-03 | 2015-11-05 | (유)스템메디케어 | 중간엽 줄기세포에서 단백질의 대량 생산 방법 |
| MX2017013852A (es) | 2015-04-28 | 2019-04-15 | Texas A & M Univ Sys | Produccion escalable de vesiculas extracelulares estandarizadas, preparaciones de vesiculas extracelular y usos de estos. |
| WO2016201220A1 (en) * | 2015-06-10 | 2016-12-15 | Children's National Medical Center | Adipocyte-derived exosomes, and compositions, kits, and methods of using the same for detection and screening |
| MX2017015962A (es) | 2015-06-10 | 2018-07-06 | Univ Texas | Uso de exosomas para el tratamiento de enfermedades. |
| US11123376B2 (en) * | 2015-06-12 | 2021-09-21 | Monash University | Method of treatment |
| US11077147B2 (en) * | 2015-07-20 | 2021-08-03 | Vivex Biologics Group, Inc. | Acellular biologic composition and method of manufacture |
| CA2993224A1 (en) * | 2015-07-31 | 2017-02-09 | Zen-Bio, Inc. | Exosome compositions and use thereof for soft tissue repair |
| CA3007733A1 (en) | 2015-12-07 | 2017-06-15 | Biotime, Inc. | Methods for the re-derivation of diverse pluripotent stem cell-derived brown fat cells |
| US11253551B2 (en) | 2016-01-11 | 2022-02-22 | Cedars-Sinai Medical Center | Cardiosphere-derived cells and exosomes secreted by such cells in the treatment of heart failure with preserved ejection fraction |
| EP3922253A1 (en) * | 2016-01-15 | 2021-12-15 | Orbsen Therapeutics Limited | Sdc-2 exosome compositions and methods of isolation and use |
| CN109414340B (zh) | 2016-01-29 | 2021-09-10 | 帕格尼亚医疗公司 | 用于肺递送的干细胞或干细胞衍生物的雾化 |
| KR101843634B1 (ko) * | 2016-04-15 | 2018-03-30 | 사회복지법인 삼성생명공익재단 | 트롬빈 처리 줄기세포에서 유래된 엑소좀을 포함하는 만성폐질환 치료용 조성물 |
| US11351200B2 (en) | 2016-06-03 | 2022-06-07 | Cedars-Sinai Medical Center | CDC-derived exosomes for treatment of ventricular tachyarrythmias |
| KR20190041456A (ko) * | 2016-06-17 | 2019-04-22 | 유나이티드 쎄러퓨틱스 코포레이션 | 향상된 효능을 가진 세포외 소포 |
| US10946047B2 (en) * | 2016-06-17 | 2021-03-16 | United Therapeutics Corporation | Extracellular vesicles with enhanced potency |
| KR102464099B1 (ko) * | 2016-08-03 | 2022-11-08 | 히로시 야부키 | 다능성 간세포에 의한 허혈재관류 폐장애의 경감 및 치료 |
| US12083149B2 (en) | 2016-08-03 | 2024-09-10 | Tohoku University | Amelioration and treatment of chronic lung disease using pluripotent stem cells |
| CN109562129B (zh) * | 2016-08-05 | 2023-02-17 | 胞外体干细胞株式会社 | 含有从脂肪来源的干细胞分离的外排体作为活性成分的用于预防或治疗肺纤维化的组合物 |
| US11541078B2 (en) | 2016-09-20 | 2023-01-03 | Cedars-Sinai Medical Center | Cardiosphere-derived cells and their extracellular vesicles to retard or reverse aging and age-related disorders |
| CN110072991A (zh) * | 2016-10-13 | 2019-07-30 | Vbc控股有限责任公司 | 来源于发炎的细胞或组织的抗炎外泌体 |
| US20210024893A1 (en) * | 2016-10-13 | 2021-01-28 | Vbc Holdings Llc | Medical uses of exosomes |
| CN110520138A (zh) * | 2017-02-28 | 2019-11-29 | 洛林大学 | 从华顿氏胶获得的用于治疗脓毒症的间充质干细胞 |
| WO2018187686A1 (en) * | 2017-04-07 | 2018-10-11 | StemoniX Inc. | Method of manufacturing and purifying exosomes from non-terminally differentiated cells |
| EP3612191A4 (en) | 2017-04-19 | 2020-12-30 | Cedars-Sinai Medical Center | METHODS AND COMPOSITIONS FOR TREATING SKELETAL MUSCLE DYSTROPHY |
| TWI704923B (zh) * | 2017-06-26 | 2020-09-21 | 台灣基督長老教會馬偕醫療財團法人馬偕紀念醫院 | 包含胞外囊泡之製劑、用以製備該製劑之方法及其用途 |
| EP3648777A4 (en) | 2017-07-06 | 2021-04-21 | Children's National Medical Center | EXOSOMES AND METHODS OF USE |
| KR20190011213A (ko) * | 2017-07-24 | 2019-02-01 | 한양대학교 에리카산학협력단 | 줄기세포로부터 추출된 엑소좀을 유효성분으로 포함하는 골다공증 예방 또는 치료용 조성물 |
| US20220023347A9 (en) * | 2017-08-15 | 2022-01-27 | Children's Medical Center Corporation | Purified mesenchymal stem cell exosomes and uses thereof |
| US11471559B2 (en) * | 2017-11-20 | 2022-10-18 | Vivex Biologics Group, Inc. | Bioenergetic bone |
| US11660355B2 (en) | 2017-12-20 | 2023-05-30 | Cedars-Sinai Medical Center | Engineered extracellular vesicles for enhanced tissue delivery |
| US12146137B2 (en) | 2018-02-05 | 2024-11-19 | Cedars-Sinai Medical Center | Methods for therapeutic use of exosomes and Y-RNAS |
| CA3098514A1 (en) | 2018-04-30 | 2019-11-14 | Children's Medical Center Corporation | Mesenchymal stromal cell exosomes and uses thereof |
| US12031976B2 (en) | 2018-05-04 | 2024-07-09 | Samsung Life Public Welfare Foundation | Screening method for therapeutic substance for preventing or treating bronchopulmonary dysplasia (BPD) |
| CA3101971A1 (en) * | 2018-05-30 | 2019-12-05 | Direct Biologics Llc | A growth factor and extracellular vesicle frozen or powdered additive comprising a mesenchymal stem cell (msc) preparation and methods of use |
| JP7363003B2 (ja) * | 2018-06-11 | 2023-10-18 | ヘルス、アンド、バイオテック、フランス（アッシュ、アンド、ベー、フランス） | 間葉系幹細胞由来細胞外小胞 |
| CN112423768A (zh) * | 2018-06-29 | 2021-02-26 | 北卡罗来纳州立大学 | 通过肺球体细胞分泌因子的吸入来进行治疗性肺修复 |
| WO2020023594A1 (en) * | 2018-07-24 | 2020-01-30 | Mayo Foundation For Medical Education And Research | Compositions and methods involving transforming extracellular vesicles |
| US12029821B2 (en) * | 2018-08-10 | 2024-07-09 | Omnispirant Limited | Extracellular vesicles for inhalation |
| WO2020102684A1 (en) * | 2018-11-15 | 2020-05-22 | Washington University | Stem cell-derived extracellular vesicles and methods of use thereof |
| EP3930734A4 (en) * | 2019-02-28 | 2022-12-07 | Merakris Therapeutics, LLC | EXTRACELLULAR VESICLES DERIVED FROM AMBIENT WATER AND THEIR USES IN WOUND HEALING |
| JP7372518B2 (ja) * | 2019-06-27 | 2023-11-01 | 国立大学法人山口大学 | 骨髄由来間葉系幹細胞の培養方法 |
| US12213995B2 (en) | 2019-07-18 | 2025-02-04 | Direct Biologics, Llc | Preparations comprising mesenchymal stem cells and cannabinoids and methods of their use |
| US20220273725A1 (en) * | 2019-07-31 | 2022-09-01 | Takahiro Ochiya | Therapeutic drugs for fibrosis, inflammation, and/or aging diseases |
| US20210052641A1 (en) * | 2019-08-23 | 2021-02-25 | United Therapeutics Corporation | Pre-conditioned extracellular vesicles and methods of production |
| CN110777113B (zh) * | 2019-09-10 | 2021-09-24 | 中山大学 | 一种用于日本血吸虫感染治疗的间质干细胞处理方法 |
| KR20220131902A (ko) * | 2019-12-04 | 2022-09-29 | 유나이티드 쎄러퓨틱스 코포레이션 | 세포외 소포 및 이의 용도 |
| CN113384597A (zh) * | 2020-03-13 | 2021-09-14 | 西比曼生物科技（上海）有限公司 | 含人体细胞衍生的细胞膜外囊泡的雾化吸入制剂、制法及其应用 |
| CN111346110A (zh) * | 2020-03-17 | 2020-06-30 | 遵义医学院附属医院 | 间充质干细胞上清液在制备治疗肺细胞损伤制剂中的应用 |
| WO2021216903A1 (en) * | 2020-04-22 | 2021-10-28 | Kenneth Allen Pettine | Methods and compositions for treating inflammatory conditions associated with infectious disease |
| AU2021306640A1 (en) * | 2020-07-09 | 2023-02-09 | Exo Biologics Sa | Process for the manufacturing of protein-associated extracellular vesicles |
| WO2022008657A1 (en) | 2020-07-09 | 2022-01-13 | Exo Biologics Sa | Extracellular vesicles and compositions thereof |
| AU2021313472B2 (en) * | 2020-07-20 | 2025-10-16 | Brainstorm Cell Therapeutics Ltd. | Methods and compositions for treating lung conditions |
| CN112043686A (zh) * | 2020-09-21 | 2020-12-08 | 沈阳三禾生物科技有限公司 | 脐带间充质干细胞外泌体雾化液在制备治疗哮喘产品中的应用 |
| CN112007049A (zh) * | 2020-09-21 | 2020-12-01 | 济南磐升生物技术有限公司 | 一种用于治疗膝骨关节炎的干细胞外泌体组合物 |
| JP2023547728A (ja) * | 2020-11-06 | 2023-11-13 | アーテック メディカル ジーエムビーエイチ | 間葉系幹細胞由来の細胞外小胞およびアルファ－１アンチトリプシンを含むウイルス感染症治療用組成物 |
| EP3995130A1 (en) * | 2020-11-06 | 2022-05-11 | JunctuCell Biomed Manufacturing GmbH | A composition comprising extra-cellular vesicles from mesenchymal stem cells and alpha-1 antitrypsin for the treatment of viral infections |
| EP4252761A4 (en) * | 2020-11-26 | 2024-06-05 | Samsung Life Public Welfare Foundation | COMPOSITION FOR THE TREATMENT OF INFECTIOUS DISEASES, COMPRISING EXOSOMES DERIVED FROM THROMBIN-PROCESSED STEM CELLS |
| USD1046870S1 (en) | 2020-12-08 | 2024-10-15 | Samsung Electronics Co., Ltd. | Accessory panel for television receiver |
| CN112675203A (zh) * | 2021-02-08 | 2021-04-20 | 瑞太生物科技(沈阳)有限公司 | 一种细胞来源的外泌体在制备治疗哮喘和/或肺纤维化生物制剂中的应用 |
| CN118369417A (zh) | 2021-09-16 | 2024-07-19 | 生命技术公司 | 细胞扩增方法和其中使用的组合物 |
| EP4501338A1 (en) | 2022-03-28 | 2025-02-05 | Hiroko Science Inc. | Pharmaceutical composition containing mir-140 and method for producing same |
| CN115725499B (zh) * | 2022-08-15 | 2025-08-15 | 哈尔滨医科大学 | 一种人脐带间充质干细胞膜仿生纳米囊泡的制备方法和应用 |
| CN116897887A (zh) * | 2023-07-31 | 2023-10-20 | 湖南中医药大学 | 一种病毒性哮喘动物模型的构建方法 |
| US12502407B2 (en) | 2024-04-25 | 2025-12-23 | Direct Biologics, Llc | Treatment of fistula with bone marrow mesenchymal stem cell derived extracellular vesicles |
| WO2025250774A1 (en) * | 2024-05-31 | 2025-12-04 | Leksum LLC | Extracellular vesicles for therapy |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5486359A (en) | 1990-11-16 | 1996-01-23 | Osiris Therapeutics, Inc. | Human mesenchymal stem cells |
| FR2766205B1 (fr) * | 1997-07-16 | 2002-08-30 | Inst Nat Sante Rech Med | Nouveau procede de sensibilisation de cellules presentatrices d'antigene et nouveaux moyens pour la mise en oeuvre du procede |
| US20030221199A1 (en) * | 1998-10-20 | 2003-11-27 | Whitsett Jeffrey A. | Surfactant protein D for the prevention and diagnosis of pulmonary emphysema |
| US20040214783A1 (en) * | 2002-05-08 | 2004-10-28 | Terman David S. | Compositions and methods for treatment of neoplastic disease |
| US20080095749A1 (en) * | 2004-03-22 | 2008-04-24 | Sudeepta Aggarwal | Mesenchymal stem cells and uses therefor |
| US8021847B2 (en) | 2004-06-02 | 2011-09-20 | Proxy Life Science Holdings, Inc. | Microvesicle-based compositions and methods |
| US8337815B2 (en) * | 2004-12-23 | 2012-12-25 | Discovery Laboratories, Inc. | Pulmonary surfactant formulations |
| WO2006110582A1 (en) | 2005-04-08 | 2006-10-19 | Xcyte Therapies, Inc. | Compositions and methods for the treatment of burns and sepsis |
| BRPI0617084A2 (pt) | 2005-09-02 | 2011-07-12 | Agency Science Tech & Res | método, linhagem de célula progenitora, célula diferenciada e método para gerar uma célula diferenciada de uma célula-tronco (es) embrionária |
| US20090274665A1 (en) * | 2006-04-27 | 2009-11-05 | Cell Therapy Technologies, Inc. | Stem Cells For Treating Lung Diseases |
| EP2054506A1 (en) | 2006-08-15 | 2009-05-06 | Agency for Science, Technology and Research | Mesenchymal stem cell conditioned medium |
| EP2578081B1 (en) | 2006-10-11 | 2016-03-09 | The General Hospital Corporation | Compositions, methods, and devices for treating liver disease |
| CN102014934B (zh) | 2008-02-22 | 2014-08-20 | 新加坡科技研究局 | 间充质干细胞颗粒 |
| US20120107413A1 (en) * | 2009-07-23 | 2012-05-03 | Singapore Health Services Pte Ltd | Pre-natal mesenchymal stem cells |
| SG2014010698A (en) | 2009-11-02 | 2014-05-29 | Agency Science Tech & Res | Methods of monitoring cellular states |
| KR101189655B1 (ko) * | 2010-06-30 | 2012-10-11 | 성균관대학교산학협력단 | 줄기세포 배양액을 포함하는 폐질환 치료용 조성물 |
| CN101890050B (zh) * | 2010-07-14 | 2012-07-04 | 江苏大学 | 脐带间质干细胞来源膜性囊泡及其应用 |
| CN103210089A (zh) | 2010-08-13 | 2013-07-17 | 格拉斯哥大学大学行政评议会 | 微泡和相关的微小rna的治疗用途 |
| AU2012220872A1 (en) | 2011-02-22 | 2013-09-12 | Caris Life Sciences Switzerland Holdings Gmbh | Circulating biomarkers |
| CN103648509B (zh) * | 2011-03-11 | 2019-02-22 | 儿童医学中心公司 | 与间充质干细胞外来体相关的方法和组合物 |
| ES2691296T3 (es) | 2012-04-03 | 2018-11-26 | Reneuron Limited | Micropartículas de células madre |
| CN106714781B (zh) | 2014-05-18 | 2021-08-10 | 儿童医学中心公司 | 涉及外排体的方法和组合物 |
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