ES2569033T3 - Procesos para producir productos de fermentación - Google Patents
Procesos para producir productos de fermentación Download PDFInfo
- Publication number
- ES2569033T3 ES2569033T3 ES11768432.4T ES11768432T ES2569033T3 ES 2569033 T3 ES2569033 T3 ES 2569033T3 ES 11768432 T ES11768432 T ES 11768432T ES 2569033 T3 ES2569033 T3 ES 2569033T3
- Authority
- ES
- Spain
- Prior art keywords
- amylase
- substitutions
- reference alpha
- alpha
- processes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Proceso para producir productos de fermentación a partir de material que contiene almidón que incluye las etapas de: i) licuefacción del material que contiene almidón utilizando una alfa-amilasa; ii) sacarificación utilizando una glucoamilasa que tiene al menos 80% de identidad con el polipéptido maduro mostrado en SEC ID n.º: 9; iii) fermentación utilizando un organismo fermentador.
Description
- Alfa-amilasa de referencia A con las sustituciones V59A+Q89R+E129V+ K177L+R179E+K220P+N224L+ Q254S+1270L
- 168 21 ND
- Alfa-amilasa de referencia A con las sustituciones V59A+Q89R+E129V+ K177L+R179E+K220P+N224L+ Q254S+H274K
- >180 24 ND
- Alfa-amilasa de referencia A con las sustituciones V59A+Q89R+E129V+ K177L+R179E+K220P+N224L+ Q254S+Y276F
- 91 15 ND
- Alfa-amilasa de referencia A con las sustituciones V59A+E129V+ R157Y+K177L+R179E+K220P+ N224L+S242Q+Q254S
- 141 41 ND
- Alfa-amilasa de referencia A con las sustituciones V59A+E129V+ K177L+R179E+H208Y+K220P+ N224L+S242Q+Q254S
- >180 62 ND
- Alfa-amilasa de referencia A con las sustituciones V59A+E129V+ K177L+R179E+K220P+N224L+ S242Q+Q254S
- >180 49 >480
- Alfa-amilasa de referencia A con las sustituciones V59A+E129V+ K177L+R179E+K220P+N224L+ S242Q+Q254S+H274K
- >180 53 ND
- Alfa-amilasa de referencia A con las sustituciones V59A+E129V+ K177L+R179E+K220P+N224L+ S242Q+Q254S+Y276F
- >180 57 ND
- Alfa-amilasa de referencia A con las sustituciones V59A+E129V+ K177L+R179E+K220P+N224L+ S242Q+Q254S+D281N
- >180 37 ND
- Alfa-amilasa de referencia A con las sustituciones V59A+E129V+ K177L+R179E+K220P+N224L+ S242Q+Q254S+M284T
- >180 51 ND
- Alfa-amilasa de referencia A con las sustituciones V59A+E129V+ K177L+R179E+K220P+N224L+ S242Q+Q254S+G416V
- >180 45 ND
- Alfa-amilasa de referencia A con las sustituciones V59A+E129V+ K177L+R179E+K220P+N224L+ Q254S
- 143 21 >480
- Alfa-amilasa de referencia A con las sustituciones V59A+E129V+ K177L+R179E+K220P+N224L+ Q254S+M284T
- >180 22 ND
- Alfa-amilasa de referencia A con las sustituciones A91L+M961+E129V+ K177L+R179E+K220P+N224L+ S242Q+Q254S
- >180 38 ND
- Alfa-amilasa de referencia A con las sustituciones E129V+K177L+ R179E
- 57 11 402
- Alfa-amilasa de referencia A con las sustituciones E129V+K177L+ R179E+K220P+N224L+S242Q+ Q254S
- 174 44 >480
- Alfa-amilasa de referencia A con las sustituciones E129V+K177L+R179E+K220P+N224L+S242Q+ Q254S+Y276F+L427M
- >180 49 >480
- Alfa-amilasa de referencia A con las sustituciones E129V+K177L+ R179E+K220P+N224L+S242Q+ Q254S+M284T
- >180 49 >480
- Alfa-amilasa de referencia A con las sustituciones E129V+K177L+ R179E+K220P+N224L+S242Q+ Q254S+N376*+I377*
- 177 36 >480
- Alfa-amilasa de referencia A con las sustituciones E129V+K177L+ R179E+K220P+N224L+Q254S
- 94 13 >480
- Alfa-amilasa de referencia A con las sustituciones E129V+K177L+ R179E+K220P+N224L+Q254S+ M284T
- 129 24 >480
- Alfa-amilasa de referencia A con las sustituciones E129V+K177L+ R179E+S242Q
- 148 30 >480
- Alfa-amilasa de referencia A con las sustituciones E129V+K177L+ R179V
- 78 9 >480
- Alfa-amilasa de referencia A con las sustituciones E129V+K177L+R179V+K220P+N224L+S242Q+ Q254S
- 178 31 >480
- Alfa-amilasa de referencia A con las sustituciones K220P+N224L+ S242Q+Q254S
- 66 17 >480
- Alfa-amilasa de referencia A con las sustituciones K220P+N224L+ Q254S
- 30 6 159
- Alfa-amilasa de referencia A con la sustitución M284T
- 35 7 278
- Alfa-amilasa de referencia A con las sustituciones M284V
- 59 13 ND
- ND no determinado
- [0118] Los resultados demuestran que las variantes de alfa-amilasa tienen significativamente superior a las de la alfa-amilasa de referencia.
- una vida media y una estabilidad
- 5
- Ejemplo 2
- Preparación de variantes de proteasa y prueba de termoestabilidad
- 10
- [0119] Los productos químicos usados fueron productos comerciales de al menos calidad reactiva.
- 15
- JTP095
- N26R/T46R/D79L/S87P/A112P/D142L 62%
- JTP096
- T46R/D79L/S87P/T116V/D142L 67%
- JTP099
- D79L/P81R/S87P/A112P/D142L 80%
- JTP101
- A27K/D79L/S87P/A112P/T124V/D142L 81%
- JTP116
- D79L/Y82F/S87P/A112P/T124V/D142L 59%
- JTP117
- D79L/Y82F/S87P/A112P/T124V/D142L 94%
- JTP127
- D79L/S87P/A112P/T124V/A126V/D142L 53%
- JTP050
- D79L S87P A112P D142L 23% 9%
- JTP134
- D79L Y82F S87P A112P D142L 40%
- JTP135
- S38T D79L S87P A112P A126V D142L 62%
- JTP136
- D79L Y82F S87P A112P A126V D142L 59%
- JTP137
- A27K D79L S87P A112P A126V D142L 54%
- JTP145
- S49P D79L S87P A112P D142L 59%
- JTP146
- S50P D79L S87P A112P D142L 63%
- JTP148
- D79L S87P D104P A112P D142L 64%
- JTP161
- D79L Y82F S87G A112P D142L 30% 12%
- JTP180
- S70V D79L Y82F S87G Y97W A112P D142L 52%
- JTP181
- D79L Y82F S87G Y97W D104P A112P D142L 45%
- JTP187
- S70V D79L Y82F S87G A112P D142L 45%
- JTP188
- D79L Y82F S87G D104P A112P D142L 43%
- JTP189
- D79L Y82F S87G A112P A126V D142L 46%
- JTP193
- Y82F S87G S70V D79L D104P A112P D142L 15%
- JTP194
- Y82F S87G D79L D104P A112P A126V D142L 22%
- JTP196
- A27K D79L Y82F S87G D104P A112P A126V D142L 18%
- Tabla 4. Actividad relativa de variantes sustitución(es) comienza desde N-terminal aminoácidos 1 a 177 de SEC ID n.º: 3.
- de proteasa. Numeración de del péptido maduro en los
- Variante
- Sustituciones Actividad relativa
- 80°C/70°C
- JTP196
- A27K D79L Y82F S87G A112P A126V D142L D104P 55%
- JTP210
- A27K Y82F S87G D104P A126V D142L A112P 36%
- JTP211
- A27K D79L Y82F D104P A126V D142L A112P 44%
- JTP213
- A27K Y82F D104P A112P D142L A126V 37%
5 Perfil de temperatura de variantes de proteasa seleccionadas utilizando enzimas purificadas
[0138] Las variantes de proteasa seleccionadas que mostraron buena termoestabilidad fueron purificadas y las enzimas purificadas fueron usadas en un ensayo de ceína-BCA se describe más adelante. La actividad de la
10 proteasa restante fue determinada a 60°C tras la incubación de la enzima a temperaturas elevadas como se indica durante 60 min.
Ensayo de ceína-BCA:
19
De los resultados se puede observar que la temperatura óptima para la glucoamilasa de Penicillium oxalicum en las condiciones dadas es entre 50°C y 70°C y la glucoamilasa mantiene más del 80% de la actividad a 95°C.
5 [0147] Termoestabilidad. Para valorar la termoestabilidad de la glucoamilasa de Penicillium oxalicum el ensayo de condición de reacción fue modificado en que la solución enzimática y el tampón de acetato fue preincubado durante 15 min a 20, 30, 40, 50, 60, 70, 75, 80, 85, 90 y 95°C. Después de la incubación, 20 microL de almidón se añadió a la solución y el ensayo fue realizado como se ha descrito anteriormente.
10 [0148] Los resultados se muestran en la tabla 7.
Tabla 7 Termoestabilidad
- Temperatura (°C)
- 20 30 40 50 60 70 80 85 90 95
- Actividad relativa (%)
- 91,0 92,9 88,1 100,0 96,9 86,0 34,8 36,0 34,2 34,8
15 De los resultados se puede observar que la glucoamilasa de Penicillium oxalicum es estable hasta 70 °C después de la preincubación durante 15 min en que mantiene más del 80% de la actividad.
[0149] PH óptimo. Para valorar el pH óptimo de la glucoamilasa de Penicillium oxalicum, el ensayo de condición de reacción anteriormente descrito fue realizado a pH 2,0, 3,0, 3,5, 4,0, 4,5, 5,0, 6,0, 7,0, 8,0, 9,0, 10,0 y 11,0. En vez
20 de usar el tampón de acetato descrito en el ensayo de condición de reacción, se usó el siguiente tampón 100mM de ácido succínico, HEPES, CHES, CAPSO, 1mM CaCl2,150mM KCI, 0,01% Tritón X-100, pH ajustado a 2,0, 3,0, 3,5, 4,0, 4,5, 5,0, 6,0 7,0, 8,0, 9,0,10,0 o 11,0 con HCl o NaOH.
[0150] Los resultados se muestran en la tabla 8. 25 Tabla 8 pH óptimo
- pH
- 2,0 3,0 3,5 4,0 4,5 5,0 6,0 7,0 8,0 9,0 10,0 11,0
- Actividad relativa (%)
- 71,4 78,6 77,0 91,2 84,2 100,0 55,5 66,7 30,9 17,8 15,9 16,1
De los resultados se puede observar que la glucoamilasa de Penicillium oxalicum en las condiciones dadas tiene la 30 actividad máxima en pH 5,0. La glucoamilasa de Penicillium oxalicum es activa en un margen de pH amplio en el que mantiene más del 50% de la actividad de pH 2 a 7.
[0151] Estabilidad de pH. Para valorar la termoestabilidad de la glucoamilasa de Penicillium oxalicum, el ensayo de condición de reacción fue modificado en que la solución enzimática (50 micro g/mL) fue preincubada durante 20
35 horas en tampones con pH 2,0, 3,0, 3,5, 4,0, 4,5, 5,0, 6,0 7,0, 8,0, 9,0,10,0 y 11,0 utilizando los tampones descritos bajo pH óptimo. Después de la preincubación, 20 microL de almidón soluble a un volumen final de 100 microL se añadió a la solución y el ensayo fue realizado como se ha descrito anteriormente.
[0152] Los resultados se muestran en la tabla 9. 40 Tabla 9 estabilidad de pH
- pH
- 2,0 3,0 3,5 4,0 4,5 5,0 6,0 7,0 8,0 9,0 10,0 11,0
- Actividad relativa (%)
- 17,4 98,0 98,0 103,2 100,0 93,4 71,2 90,7 58,7 17,4 17,0 17,2
De los resultados se puede observar que la glucoamilasa de Penicillium oxalicum es estable de pH 3 a pH 7 45 después de la preincubación durante 20 horas y reduce su actividad a pH 8.
[0153] Este ejemplo describe un proceso de producción de etanol donde la glucoamilasa de Penicillium oxalicum
50 termoestable se usa en un proceso de pre-sacarificación seguido de un proceso de fermentación sin usar glucoamilasa adicional. Este proceso se compara con un proceso de producción de etanol tradicional donde el proceso de licuefacción es seguido de un proceso de SSF utilizando una glucoamilasa no termoestable.
21
Claims (1)
-
imagen1 imagen2
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
WOPCT/CN2010/071753 | 2010-04-14 | ||
CN2010071753 | 2010-04-14 | ||
PCT/CN2011/072732 WO2011127820A1 (en) | 2010-04-14 | 2011-04-13 | Processes for producing fermentation products |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2569033T3 true ES2569033T3 (es) | 2016-05-06 |
Family
ID=44798280
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES11768414.2T Active ES2565060T3 (es) | 2010-04-14 | 2011-04-11 | Polipéptidos que tienen actividad de glucoamilasa y polinucleótidos que codifican los mismos |
ES11768432.4T Active ES2569033T3 (es) | 2010-04-14 | 2011-04-13 | Procesos para producir productos de fermentación |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES11768414.2T Active ES2565060T3 (es) | 2010-04-14 | 2011-04-11 | Polipéptidos que tienen actividad de glucoamilasa y polinucleótidos que codifican los mismos |
Country Status (8)
Country | Link |
---|---|
US (1) | US20130095522A1 (es) |
EP (2) | EP2558484B1 (es) |
AU (1) | AU2011239257B2 (es) |
CA (1) | CA2795806C (es) |
DK (1) | DK2558484T3 (es) |
ES (2) | ES2565060T3 (es) |
MX (1) | MX338068B (es) |
WO (2) | WO2011127802A1 (es) |
Families Citing this family (75)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103068975B (zh) * | 2010-01-04 | 2018-03-20 | 诺维信公司 | α‑淀粉酶变体和编码该变体的多核苷酸 |
US9617527B2 (en) | 2010-04-14 | 2017-04-11 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
ES2673940T3 (es) | 2010-12-22 | 2018-06-26 | Novozymes North America, Inc. | Proceso para producir productos de fermentación a partir de materiales que contienen almidón |
CN103502420B (zh) | 2011-04-15 | 2020-02-04 | 诺维信公司 | 生产啤酒麦汁的方法 |
WO2013036526A1 (en) | 2011-09-06 | 2013-03-14 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
WO2013055676A1 (en) * | 2011-10-11 | 2013-04-18 | Novozymes North America, Inc. | Processes for producing fermentation products |
WO2013053801A1 (en) | 2011-10-11 | 2013-04-18 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
IN2014CN04905A (es) | 2011-12-02 | 2015-09-18 | Novozymes As | |
ES2935920T3 (es) | 2012-03-30 | 2023-03-13 | Novozymes North America Inc | Procesos de elaboración de productos de fermentación |
BR112015008408A2 (pt) | 2012-10-17 | 2017-08-08 | Novozymes As | método de produção de um mosto cervejeiro |
EP2935574B1 (en) * | 2012-12-17 | 2019-11-13 | Novozymes A/S | Alpha-amylases and polynucleotides encoding same |
US9695406B2 (en) | 2013-03-07 | 2017-07-04 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
HUE057274T2 (hu) | 2013-06-24 | 2022-04-28 | Novozymes As | Eljárás olaj kinyerésére fermentációs termék elõállítására szolgáló eljárásokból és eljárások fermentációs termékek elõállítására |
US11939552B2 (en) | 2013-06-24 | 2024-03-26 | Novozymes A/S | Process of recovering oil |
CA2915336C (en) | 2013-07-17 | 2024-03-12 | Novozymes A/S | Pullulanase chimeras and polynucleotides encoding same |
US9951364B2 (en) | 2013-09-11 | 2018-04-24 | Novozymes A/S | Processes for producing fermentation products |
DK3097192T3 (en) | 2014-01-22 | 2018-11-19 | Novozymes As | PULLULANASE VARIATIONS AND POLYNUCLEOTIDES CODING THEM |
RU2019134734A (ru) * | 2014-02-07 | 2019-11-18 | Новозимс А/С | Композиции для получения глюкозных сиропов |
ES2712402T3 (es) | 2014-06-25 | 2019-05-13 | Novozymes As | Variantes de xilanasa y polinucleótidos que las codifican |
US20160007618A1 (en) * | 2014-07-08 | 2016-01-14 | Caravan Ingredients Inc. | Sugar-producing and texture-improving bakery methods and products formed therefrom |
US10584324B2 (en) | 2014-10-23 | 2020-03-10 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
WO2016087445A1 (en) | 2014-12-01 | 2016-06-09 | Novozymes A/S | Improved production of glucose syrups |
WO2016196202A1 (en) | 2015-05-29 | 2016-12-08 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
CN108350442A (zh) | 2015-06-18 | 2018-07-31 | 诺维信公司 | 具有海藻糖酶活性的多肽及其在产生发酵产物的方法中的用途 |
US20180208916A1 (en) | 2015-07-23 | 2018-07-26 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2017112539A1 (en) | 2015-12-22 | 2017-06-29 | Novozymes A/S | Process of extracting oil from thin stillage |
WO2018015444A1 (en) | 2016-07-22 | 2018-01-25 | Novozymes A/S | Crispr-cas9 genome editing with multiple guide rnas in filamentous fungi |
CN109476714A (zh) | 2016-07-22 | 2019-03-15 | 诺维信公司 | 改善的丝状真菌宿主 |
WO2018093285A1 (en) | 2016-11-18 | 2018-05-24 | Baltika Breweries - Part Of The Carlsberg Group | Method of producing a grain malt and the malt product obtained in this way |
WO2018098381A1 (en) | 2016-11-23 | 2018-05-31 | Novozymes A/S | Improved yeast for ethanol production |
US20190367952A1 (en) | 2016-11-23 | 2019-12-05 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
CA3055530A1 (en) * | 2017-03-07 | 2018-09-13 | Danisco Us Inc. | Thermostable glucoamylase and methods of use, thereof |
BR112019025391A2 (pt) | 2017-06-02 | 2020-07-07 | Novozymes A/S | levedura melhorada para a produção de etanol |
EP4015524A1 (en) | 2017-06-28 | 2022-06-22 | Novozymes A/S | Polypeptides having trehalase activity and polynucleotides encoding same |
US11220679B2 (en) | 2017-08-08 | 2022-01-11 | Novozymes A/S | Polypeptides having trehalase activity |
WO2019046232A1 (en) | 2017-08-30 | 2019-03-07 | Novozymes A/S | COMBINED USE OF ENDOPROTEASE OF FAMILY M35 AND EXOPROTEASE OF FAMILY S53 IN FERMENTATION OF STARCH |
CA3070730A1 (en) | 2017-09-15 | 2019-03-21 | Novozymes A/S | Enzyme blends and processes for improving the nutritional quality of animal feed |
WO2019070883A1 (en) | 2017-10-04 | 2019-04-11 | Novozymes A/S | POLYPEPTIDES WITH PROTEASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME |
EP3701037A1 (en) | 2017-10-23 | 2020-09-02 | Novozymes A/S | Processes for reducing lactic acid in a biofuel fermentation system |
ES2955269T3 (es) | 2017-12-08 | 2023-11-29 | Novozymes As | Variantes de alfa-amilasa y polinucleótidos que codifican las mismas |
DK3720956T5 (da) | 2017-12-08 | 2024-08-26 | Novozymes As | Alfa-amylasevarianter og polynukleotider, der koder for dem |
CN113286871A (zh) | 2018-01-29 | 2021-08-20 | 诺维信公司 | 用于乙醇生产的氮利用提高的微生物 |
AU2019222480A1 (en) | 2018-02-15 | 2020-10-08 | Microbiogen Pty. Ltd. | Improved yeast for ethanol production |
WO2019197318A1 (en) | 2018-04-09 | 2019-10-17 | Novozymes A/S | Polypeptides having alpha-amylase activity and polynucleotides encoding same |
WO2019231944A2 (en) | 2018-05-31 | 2019-12-05 | Novozymes A/S | Processes for enhancing yeast growth and productivity |
BR112021000369A2 (pt) | 2018-07-11 | 2021-04-13 | Novozymes A/S | Processos para produção de produtos de fermentação |
CN112601818A (zh) | 2018-07-25 | 2021-04-02 | 诺维信公司 | 用于生产乙醇的表达酶的酵母 |
US11807889B2 (en) | 2018-10-08 | 2023-11-07 | Novozymes A/S | Yeast expressing a heterologous phospholipase for ethanol production |
BR112021014873A2 (pt) | 2019-01-31 | 2021-10-05 | Novozymes A/S | Polipeptídeo, combinação de enzimas, polinucleotídeo, construto de ácido nucleico ou vetor de expressão recombinante, célula hospedeira recombinante, método de produção de um polipeptídeo, e, processo de produção de um produto de fermentação |
US20220154226A1 (en) | 2019-03-18 | 2022-05-19 | Novozymes A/S | Polypeptides Having Pullulanase Activity Suitable For Use In Liquefaction |
BR112021017085A2 (pt) | 2019-04-02 | 2022-02-08 | Novozymes As | Processo de produção de um produto de fermentação |
CN114450390A (zh) | 2019-07-26 | 2022-05-06 | 诺维信公司 | 用于乙醇生产的氮转运提高的微生物 |
CA3144423A1 (en) | 2019-08-05 | 2021-02-11 | Kurt Creamer | Enzyme blends and processes for producing a high protein feed ingredient from a whole stillage byproduct |
WO2021025872A1 (en) | 2019-08-06 | 2021-02-11 | Novozymes A/S | Fusion proteins for improved enzyme expression |
MX2022002834A (es) | 2019-09-16 | 2022-04-06 | Novozymes As | Polipeptidos con actividad beta-glucanasa y polinucleotidos que los codifican. |
BR112022011271A2 (pt) | 2019-12-10 | 2022-09-06 | Novozymes As | Célula hospedeira recombinante, composição, métodos para produzir um derivado de uma célula e um produto de fermentação, e, uso de uma célula hospedeira recombinante |
WO2021126966A1 (en) | 2019-12-16 | 2021-06-24 | Novozymes A/S | Processes for producing fermentation products |
US20240228996A1 (en) | 2020-02-10 | 2024-07-11 | Novozymes A/S | Polypeptides having alpha-amylase activity and polynucleotides encoding same |
US20230142991A1 (en) | 2020-02-10 | 2023-05-11 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
CN111269950B (zh) * | 2020-02-26 | 2022-03-08 | 安徽丰原发酵技术工程研究有限公司 | 发酵生产l-苹果酸的培养基及其制备方法与应用 |
CN115427560A (zh) | 2020-03-12 | 2022-12-02 | 诺维信公司 | 使用无催化活性rna指导的内切核酸酶的crispr-aid |
BR112023005131A2 (pt) * | 2020-09-21 | 2023-04-25 | Dupont Nutrition Biosci Aps | Processo para fazer um produto de panificação com resiliência melhorada, produto de panificação, uso de uma exoamilase não maltogênica e uma glucoamilase, composição melhoradora para uma massa e massa |
AU2021370998A1 (en) | 2020-11-02 | 2023-05-25 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
AU2021372822A1 (en) * | 2020-11-02 | 2023-06-01 | Novozymes A/S | Baked and par-baked products with thermostable amg variants from penicillium |
EP4237430A1 (en) | 2020-11-02 | 2023-09-06 | Novozymes A/S | Leader peptides and polynucleotides encoding the same |
WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
WO2022251056A1 (en) | 2021-05-27 | 2022-12-01 | Novozymes A/S | Transcriptional regulators and polynucleotides encoding the same |
CN117795089A (zh) | 2021-06-07 | 2024-03-29 | 诺维信公司 | 用于改善的乙醇发酵的工程化微生物 |
WO2023208403A1 (en) * | 2022-04-24 | 2023-11-02 | Novozymes A/S | Method for preparing steamed flour-based products by using a thermostable glucoamylase |
WO2024118096A1 (en) * | 2022-11-30 | 2024-06-06 | Novozymes A/S | Baking at low-ph with thermostable glucoamylase variants |
WO2024137248A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Compositions comprising arabinofuranosidases and a xylanase, and use thereof for increasing hemicellulosic fiber solubilization |
WO2024137704A2 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast |
WO2024137252A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Process for reducing syrup viscosity in the backend of a process for producing a fermentation product |
WO2024137250A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same |
WO2024137246A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Carbohydrate esterase family 1 (ce1) polypeptides having ferulic acid esterase and/or acetyl xylan esterase activity and polynucleotides encoding same |
Family Cites Families (67)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK110031A (es) | 1965-12-02 | |||
JPS5534046A (en) | 1978-09-01 | 1980-03-10 | Cpc International Inc | Novel glucoamyrase having excellent heat resistance and production |
DK145679A (da) | 1979-04-09 | 1980-10-10 | Novo Industri As | Fremgangsmaade til termisk destabilisering af mikrobiel osteloebe |
US4560651A (en) | 1981-04-20 | 1985-12-24 | Novo Industri A/S | Debranching enzyme product, preparation and use thereof |
NO840200L (no) | 1983-01-28 | 1984-07-30 | Cefus Corp | Glukoamylase cdna. |
DK135983D0 (da) | 1983-03-25 | 1983-03-25 | Novo Industri As | Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse |
US4536477A (en) | 1983-08-17 | 1985-08-20 | Cpc International Inc. | Thermostable glucoamylase and method for its production |
US4587215A (en) | 1984-06-25 | 1986-05-06 | Uop Inc. | Highly thermostable amyloglucosidase |
US4628031A (en) | 1984-09-18 | 1986-12-09 | Michigan Biotechnology Institute | Thermostable starch converting enzymes |
JPS62126989A (ja) | 1985-11-26 | 1987-06-09 | Godo Shiyusei Kk | コルテイシウム属担子菌の生産する酵素を用いた澱粉質の無蒸煮糖化法 |
DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
DK564086A (da) | 1986-11-25 | 1988-06-17 | Novo Industri As | Enzymatisk detergent-additiv |
WO1989001969A1 (en) | 1987-09-04 | 1989-03-09 | Novo-Nordisk A/S | Process for the production of protein products in aspergillus and promoters for use in aspergillus |
US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
IL97645A (en) | 1990-03-23 | 1997-03-18 | Gist Brocades Nv | Production of enzymes in seeds and their use |
US5162210A (en) | 1990-06-29 | 1992-11-10 | Iowa State University Research Foundation | Process for enzymatic hydrolysis of starch to glucose |
WO1992002614A1 (en) | 1990-08-01 | 1992-02-20 | Novo Nordisk A/S | Novel thermostable pullulanases |
US6395966B1 (en) | 1990-08-09 | 2002-05-28 | Dekalb Genetics Corp. | Fertile transgenic maize plants containing a gene encoding the pat protein |
IL99552A0 (en) | 1990-09-28 | 1992-08-18 | Ixsys Inc | Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof |
DK47493D0 (da) | 1993-04-27 | 1993-04-27 | Novo Nordisk As | Fremgangsmaade til fremstilling af ost |
DK81293D0 (da) | 1993-07-06 | 1993-07-06 | Novo Nordisk As | Enzym |
DK81193D0 (da) | 1993-07-06 | 1993-07-06 | Novo Nordisk As | Enzym |
DE4343591A1 (de) | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
CN1192108C (zh) | 1994-06-03 | 2005-03-09 | 诺沃奇梅兹生物技术有限公司 | 纯化的毁丝霉属漆酶及编码该酶的核酸 |
ATE294871T1 (de) | 1994-06-30 | 2005-05-15 | Novozymes Biotech Inc | Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren |
AR000862A1 (es) | 1995-02-03 | 1997-08-06 | Novozymes As | Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del |
US6093562A (en) | 1996-02-05 | 2000-07-25 | Novo Nordisk A/S | Amylase variants |
DE69637940D1 (de) | 1995-02-03 | 2009-07-09 | Novozymes As | Eine methode zum entwurf von alpha-amylase mutanten mit vorbestimmten eigenschaften |
EP0904360B1 (en) | 1996-04-30 | 2013-07-31 | Novozymes A/S | alpha-AMYLASE MUTANTS |
AU9434398A (en) | 1997-10-13 | 1999-05-03 | Novo Nordisk A/S | Alpha-amylase mutants |
JP4718005B2 (ja) | 1997-11-26 | 2011-07-06 | ノボザイムス アクティーゼルスカブ | 熱安定グルコアミラーゼ |
US6255084B1 (en) * | 1997-11-26 | 2001-07-03 | Novozymes A/S | Thermostable glucoamylase |
CN103352033B (zh) | 1998-02-27 | 2016-05-11 | 诺维信公司 | 麦芽α淀粉酶变体 |
WO2000004136A1 (en) | 1998-07-15 | 2000-01-27 | Novozymes A/S | Glucoamylase variants |
AU6188599A (en) | 1998-10-26 | 2000-05-15 | Novozymes A/S | Constructing and screening a dna library of interest in filamentous fungal cells |
EP2278016B1 (en) | 1999-03-22 | 2012-09-26 | Novozymes Inc. | Promoter sequences derived from Fusarium Venenatum and uses thereof |
EP1818396B1 (en) | 1999-03-30 | 2014-06-18 | Novozymes A/S | Alpha-amylase variants |
AU5061000A (en) * | 1999-06-02 | 2000-12-28 | Novozymes A/S | Novel glucoamylase |
WO2001004273A2 (en) | 1999-07-09 | 2001-01-18 | Novozymes A/S | Glucoamylase variant |
EP1250423B1 (en) | 2000-01-12 | 2008-09-03 | Novozymes A/S | Pullulanase variants and methods for preparing such variants with predetermined properties |
CA2402195A1 (en) | 2000-04-07 | 2001-10-18 | Novozymes A/S | Signal sequence trapping |
ES2248328T3 (es) | 2000-06-02 | 2006-03-16 | Novozymes A/S | Variantes de cutinasa. |
US20020155574A1 (en) | 2000-08-01 | 2002-10-24 | Novozymes A/S | Alpha-amylase mutants with altered properties |
EP2308979A3 (en) | 2000-08-01 | 2011-05-04 | Novozymes A/S | Alpha-amylase mutants with altered properties |
US7151204B2 (en) | 2001-01-09 | 2006-12-19 | Monsanto Technology Llc | Maize chloroplast aldolase promoter compositions and methods for use thereof |
EP1434861A2 (en) * | 2001-10-01 | 2004-07-07 | Novozymes A/S | Glucoamylase variants |
RU2318018C2 (ru) | 2001-12-07 | 2008-02-27 | Новозимс А/С | Полипептиды, обладающие протеазной активностью, и нуклеиновые кислоты, кодирующие указанные полипептиды |
US20030180900A1 (en) * | 2002-02-08 | 2003-09-25 | Oreste Lantero | Methods for producing ethanol from carbon substrates |
CN1788083B (zh) | 2003-03-10 | 2011-10-05 | 诺维信公司 | 酒精产品生产方法 |
WO2004111218A2 (en) * | 2003-06-13 | 2004-12-23 | Novozymes A/S | Method for producing glucoamylases and their uses |
WO2005003311A2 (en) | 2003-06-25 | 2005-01-13 | Novozymes A/S | Enzymes for starch processing |
WO2004113551A1 (en) | 2003-06-25 | 2004-12-29 | Novozymes A/S | Process for the hydrolysis of starch |
MXPA06004463A (es) | 2003-10-28 | 2006-06-27 | Novozymes North America Inc | Enzimas hibridas. |
EP1682655B1 (en) | 2003-10-30 | 2009-09-02 | Novozymes A/S | Carbohydrate-binding modules |
WO2006017294A1 (en) * | 2004-07-13 | 2006-02-16 | Novozymes North America, Inc | Liquefaction process |
WO2006069289A2 (en) | 2004-12-22 | 2006-06-29 | Novozymes North America, Inc | Polypeptides having glucoamylase activity and polynucleotides encoding same |
WO2006086792A2 (en) * | 2005-02-07 | 2006-08-17 | Novozymes North America, Inc. | Fermentation product production processes |
US7871800B2 (en) | 2006-04-19 | 2011-01-18 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
ATE448319T1 (de) | 2006-06-15 | 2009-11-15 | Novozymes As | Verfahren zur herstellung eines stärkehydrolysats |
EP2193195B1 (en) | 2007-09-18 | 2014-12-17 | Novozymes A/S | Polypeptides having tyrosinase activity and polynucleotides encoding same |
PL2514818T3 (pl) * | 2007-10-09 | 2014-11-28 | Danisco Us Inc | Odmiany glukoamylazy |
PL2201108T3 (pl) * | 2007-10-18 | 2015-03-31 | Danisco Us Inc | Mieszanki enzymów do fermentacji |
WO2009061381A2 (en) | 2007-11-05 | 2009-05-14 | Danisco Us Inc., Genencor Division | Alpha-amylase variants with altered properties |
ES2527586T3 (es) * | 2007-11-20 | 2015-01-27 | Danisco Us Inc. | Variantes de glucoamilasa con propiedades modificadas |
CA2726688A1 (en) | 2008-06-23 | 2010-01-21 | Novozymes A/S | Processes for producing fermentation products |
WO2010036515A1 (en) | 2008-09-25 | 2010-04-01 | Danisco Us Inc. | Alpha-amylase blends and methods for using said blends |
-
2011
- 2011-04-11 DK DK11768414.2T patent/DK2558484T3/en active
- 2011-04-11 WO PCT/CN2011/072598 patent/WO2011127802A1/en active Application Filing
- 2011-04-11 ES ES11768414.2T patent/ES2565060T3/es active Active
- 2011-04-11 MX MX2012011774A patent/MX338068B/es active IP Right Grant
- 2011-04-11 AU AU2011239257A patent/AU2011239257B2/en active Active
- 2011-04-11 EP EP11768414.2A patent/EP2558484B1/en active Active
- 2011-04-11 CA CA2795806A patent/CA2795806C/en active Active
- 2011-04-13 WO PCT/CN2011/072732 patent/WO2011127820A1/en active Application Filing
- 2011-04-13 ES ES11768432.4T patent/ES2569033T3/es active Active
- 2011-04-13 US US13/639,233 patent/US20130095522A1/en not_active Abandoned
- 2011-04-13 EP EP11768432.4A patent/EP2558584B1/en not_active Not-in-force
Also Published As
Publication number | Publication date |
---|---|
CA2795806C (en) | 2019-01-08 |
ES2565060T3 (es) | 2016-03-31 |
EP2558584A1 (en) | 2013-02-20 |
MX2012011774A (es) | 2012-12-17 |
US20130095522A1 (en) | 2013-04-18 |
EP2558484A1 (en) | 2013-02-20 |
EP2558584A4 (en) | 2013-12-04 |
EP2558584B1 (en) | 2016-02-24 |
WO2011127802A1 (en) | 2011-10-20 |
AU2011239257A1 (en) | 2012-08-09 |
CA2795806A1 (en) | 2011-10-20 |
DK2558484T3 (en) | 2016-03-29 |
WO2011127820A1 (en) | 2011-10-20 |
MX338068B (es) | 2016-04-01 |
EP2558484A4 (en) | 2013-12-04 |
AU2011239257B2 (en) | 2015-06-25 |
EP2558484B1 (en) | 2016-01-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ES2569033T3 (es) | Procesos para producir productos de fermentación | |
Jansen et al. | Towards large scale fermentative production of succinic acid | |
ES2627987T3 (es) | Proceso de licuefacción para producir productos de fermentación | |
AR093680A1 (es) | Procesos para la produccion de productos de fermentacion | |
JP2013511280A5 (es) | ||
FI3720955T3 (fi) | Alfa-amylaasivariantteja ja niitä koodaavia polynukleotideja | |
WO2006086792A3 (en) | Fermentation product production processes | |
WO2008153815A3 (en) | Variants of an alpha-amylase with improved production levels in fermentation processes | |
AR090949A1 (es) | METODO DE USAR a-AMILASA DE ASPERGILLUS CLAVATUS PARA LA SACARIFICACION | |
CO6270375A2 (es) | Degradacion enzimatica de sustratos de biomasa que comprenden manano | |
RU2012133453A (ru) | Альфа-амилазы | |
MX2012001243A (es) | Combinacion de alfa-amilasa para procesamiento de almidon y metodo de uso de la misma. | |
MY161108A (en) | Fermentation medias and processes thereof | |
JP2016500267A5 (es) | ||
Jimenez-Quero et al. | Fungal fermentation of lignocellulosic biomass for itaconic and fumaric acid production | |
CN103981239B (zh) | 一种提高高浓度淀粉糖化产物纯度的方法 | |
BR112012019838A2 (pt) | metodo para a producao de um produto de fermentacao a partir de uma materia-prima lignocelulosica | |
RU2013150787A (ru) | Способ получения пивного сусла | |
MY166434A (en) | Mutant beta-glucosidase, enzyme composition for decomposing biomass, and method of producing sugar solution | |
BR112014000247A8 (pt) | Composição, método para degradação de uma biomassa lignocelulósica, método para produção de um produto de fermentação, método para produzir ácido glucônico, ácido xilônico e/ou ácido xilobiônico, método para aumentar a produção de ácido glucônico, ácido xilônico e/ou ácido xilobiônico, método para aumentar a produção de açúcares e uso da composição | |
ES2625501T3 (es) | Procedimiento mejorado de producción de enzimas celulolíticas y/o hemicelulolíticas | |
WO2010098694A3 (en) | Abe fermentation by simultaneous saccharification and fermentation, wherein the products are separated empoying a liquid membrane | |
RU2009136073A (ru) | Способ получения мальтозного сиропа | |
BR112014026885A2 (pt) | método para produzir etanol mediante fermentação de um produto de grãos integrais | |
ES2542621B1 (es) | Polipéptidos con actividad polisacárido monooxigenasa y su uso para la producción de azúcares fermentables |