ES2546184T3 - Secuencia de ADN artificial con función líder optimizada en 5' (5'-UTR) para la expresión mejorada de proteínas heterólogas en plantas - Google Patents
Secuencia de ADN artificial con función líder optimizada en 5' (5'-UTR) para la expresión mejorada de proteínas heterólogas en plantas Download PDFInfo
- Publication number
- ES2546184T3 ES2546184T3 ES07858186.5T ES07858186T ES2546184T3 ES 2546184 T3 ES2546184 T3 ES 2546184T3 ES 07858186 T ES07858186 T ES 07858186T ES 2546184 T3 ES2546184 T3 ES 2546184T3
- Authority
- ES
- Spain
- Prior art keywords
- plants
- utr
- heterologous proteins
- artificial dna
- dna sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000014509 gene expression Effects 0.000 title abstract description 4
- 108020003589 5' Untranslated Regions Proteins 0.000 title abstract description 3
- 108090000623 proteins and genes Proteins 0.000 title abstract description 3
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 title 1
- 108020004414 DNA Proteins 0.000 abstract 1
- 230000002349 favourable effect Effects 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 101100437498 Escherichia coli (strain K12) uidA gene Proteins 0.000 description 9
- 241000196324 Embryophyta Species 0.000 description 5
- 108020004463 18S ribosomal RNA Proteins 0.000 description 4
- 238000013518 transcription Methods 0.000 description 4
- 230000035897 transcription Effects 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- 238000011529 RT qPCR Methods 0.000 description 3
- 102000053187 Glucuronidase Human genes 0.000 description 2
- 108010060309 Glucuronidase Proteins 0.000 description 2
- 238000002123 RNA extraction Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 238000011897 real-time detection Methods 0.000 description 2
- 238000013207 serial dilution Methods 0.000 description 2
- 230000009261 transgenic effect Effects 0.000 description 2
- 108091093088 Amplicon Proteins 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 238000010818 SYBR green PCR Master Mix Methods 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 102000042567 non-coding RNA Human genes 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
- A01H6/4636—Oryza sp. [rice]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Animal Behavior & Ethology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Pharmacology & Pharmacy (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Physiology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITUD20060280 | 2006-12-29 | ||
| IT000280A ITUD20060280A1 (it) | 2006-12-29 | 2006-12-29 | Sequenza artificiale di dna evente funzione di leader in 5' (5'-utr) ottimizzata per la sovraespressione di proteine eterologhe in pianta |
| PCT/EP2007/064590 WO2008080954A1 (en) | 2006-12-29 | 2007-12-27 | Artificial dna sequence with optimized leader function in 5' (5'-utr) for the improved expression of heterologous proteins in plants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2546184T3 true ES2546184T3 (es) | 2015-09-21 |
Family
ID=39205172
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES07858186.5T Active ES2546184T3 (es) | 2006-12-29 | 2007-12-27 | Secuencia de ADN artificial con función líder optimizada en 5' (5'-UTR) para la expresión mejorada de proteínas heterólogas en plantas |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US9085775B2 (enExample) |
| EP (1) | EP2118291B1 (enExample) |
| JP (2) | JP2010514432A (enExample) |
| KR (1) | KR101596229B1 (enExample) |
| CN (1) | CN101617048B (enExample) |
| AU (1) | AU2007341217A1 (enExample) |
| BR (1) | BRPI0720641A8 (enExample) |
| CA (1) | CA2674170C (enExample) |
| CY (1) | CY1116649T1 (enExample) |
| DK (1) | DK2118291T3 (enExample) |
| ES (1) | ES2546184T3 (enExample) |
| HU (1) | HUE027484T2 (enExample) |
| IT (1) | ITUD20060280A1 (enExample) |
| NZ (1) | NZ578518A (enExample) |
| PL (1) | PL2118291T3 (enExample) |
| PT (1) | PT2118291E (enExample) |
| RU (1) | RU2009129091A (enExample) |
| WO (1) | WO2008080954A1 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ITUD20080055A1 (it) * | 2008-03-13 | 2009-09-14 | Transactiva S R L | Procedimento per la produzione di una proteina umana in pianta, in particolare un enzima lisosomiale umano ricombinante in endosperma di cereali |
| KR101703540B1 (ko) * | 2012-06-04 | 2017-02-08 | 대한민국 | 3'' 영역의 염기 변이를 통한 전체 단백질의 발현 조절 방법 |
| ITUD20130002A1 (it) * | 2013-01-16 | 2014-07-17 | Transactiva S R L | Sequenza artificiale di dna avente funzione di leader in 5' (5'-utr) ottimizzata per la sovraespressione di proteine ricombinanti in pianta e metodo per la produzione di proteine ricombinanti in pianta |
| JP6609551B2 (ja) * | 2013-06-25 | 2019-11-20 | ザ ユニバーシティー オブ ユタ リサーチ ファンデーション | ポリメラーゼ連鎖反応を行う方法及びその関連する使用 |
| IT201700042052A1 (it) * | 2017-04-14 | 2018-10-14 | Transactiva S R L | Vettore di espressione e metodo per la produzione di una proteina umana in pianta, in particolare un anticorpo ricombinante in endosperma di cereale |
| CN121022840A (zh) * | 2025-02-06 | 2025-11-28 | 北京臻知医学科技有限责任公司 | 一种5’UTR序列及其在提高mRNA翻译效率中的应用 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8613481D0 (en) * | 1986-06-04 | 1986-07-09 | Diatech Ltd | Translation of mrna |
| GB9206016D0 (en) * | 1992-03-19 | 1992-04-29 | Sandoz Ltd | Improvements in or relating to organic compounds |
| US5362865A (en) | 1993-09-02 | 1994-11-08 | Monsanto Company | Enhanced expression in plants using non-translated leader sequences |
| GB9515941D0 (en) | 1995-08-03 | 1995-10-04 | Zeneca Ltd | DNA constructs |
| JP3761628B2 (ja) * | 1995-07-14 | 2006-03-29 | 住友化学株式会社 | 植物プロモーターおよびその利用 |
| GB9516241D0 (en) | 1995-08-08 | 1995-10-11 | Zeneca Ltd | Dna constructs |
| ZA9811228B (en) * | 1997-12-12 | 1999-06-14 | Mogen Int | New constitutive plant promoters |
| US6376747B1 (en) | 1999-08-27 | 2002-04-23 | Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada | Plant-derived map kinase kinase |
| FR2815969B1 (fr) * | 2000-10-30 | 2004-12-10 | Aventis Cropscience Sa | Plantes tolerantes aux herbicides par contournement de voie metabolique |
| KR100474115B1 (ko) * | 2001-06-01 | 2005-03-08 | 대한민국 | 유전자변형식물에 대한 ｐｃｒ 검정용 프라이머 |
| US7847064B2 (en) * | 2004-07-09 | 2010-12-07 | Donald Danforth Plant Science Center | Methods and compositions for regulating gene expression in plant cells |
| US7517689B2 (en) * | 2004-07-09 | 2009-04-14 | Donald Danforth Plant Science Center | Methods and compositions for regulating gene expression in plant cells |
| US8065093B2 (en) * | 2004-10-06 | 2011-11-22 | Agency For Science, Technology, And Research | Methods, systems, and compositions for classification, prognosis, and diagnosis of cancers |
-
2006
- 2006-12-29 IT IT000280A patent/ITUD20060280A1/it unknown
-
2007
- 2007-12-27 BR BRPI0720641A patent/BRPI0720641A8/pt not_active IP Right Cessation
- 2007-12-27 DK DK07858186.5T patent/DK2118291T3/en active
- 2007-12-27 PL PL07858186T patent/PL2118291T3/pl unknown
- 2007-12-27 RU RU2009129091/10A patent/RU2009129091A/ru not_active Application Discontinuation
- 2007-12-27 KR KR1020097015452A patent/KR101596229B1/ko not_active Expired - Fee Related
- 2007-12-27 AU AU2007341217A patent/AU2007341217A1/en not_active Abandoned
- 2007-12-27 CN CN2007800518216A patent/CN101617048B/zh not_active Expired - Fee Related
- 2007-12-27 EP EP07858186.5A patent/EP2118291B1/en active Active
- 2007-12-27 WO PCT/EP2007/064590 patent/WO2008080954A1/en not_active Ceased
- 2007-12-27 PT PT78581865T patent/PT2118291E/pt unknown
- 2007-12-27 CA CA2674170A patent/CA2674170C/en not_active Expired - Fee Related
- 2007-12-27 JP JP2009543473A patent/JP2010514432A/ja active Pending
- 2007-12-27 US US12/521,355 patent/US9085775B2/en not_active Expired - Fee Related
- 2007-12-27 ES ES07858186.5T patent/ES2546184T3/es active Active
- 2007-12-27 NZ NZ578518A patent/NZ578518A/xx not_active IP Right Cessation
- 2007-12-27 HU HUE07858186A patent/HUE027484T2/en unknown
-
2014
- 2014-05-21 JP JP2014105535A patent/JP5913434B2/ja active Active
-
2015
- 2015-08-26 CY CY20151100750T patent/CY1116649T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CN101617048A (zh) | 2009-12-30 |
| JP5913434B2 (ja) | 2016-04-27 |
| JP2010514432A (ja) | 2010-05-06 |
| CA2674170C (en) | 2019-03-12 |
| BRPI0720641A8 (pt) | 2017-12-19 |
| US20110041222A1 (en) | 2011-02-17 |
| CA2674170A1 (en) | 2008-07-10 |
| PT2118291E (pt) | 2015-09-24 |
| HUE027484T2 (en) | 2016-09-28 |
| KR101596229B1 (ko) | 2016-03-07 |
| WO2008080954A1 (en) | 2008-07-10 |
| PL2118291T3 (pl) | 2015-11-30 |
| EP2118291A1 (en) | 2009-11-18 |
| DK2118291T3 (en) | 2015-08-31 |
| CY1116649T1 (el) | 2017-03-15 |
| CN101617048B (zh) | 2012-11-07 |
| EP2118291B1 (en) | 2015-06-03 |
| US9085775B2 (en) | 2015-07-21 |
| KR20090102825A (ko) | 2009-09-30 |
| AU2007341217A1 (en) | 2008-07-10 |
| JP2014221040A (ja) | 2014-11-27 |
| BRPI0720641A2 (pt) | 2014-03-18 |
| NZ578518A (en) | 2012-08-31 |
| RU2009129091A (ru) | 2011-02-10 |
| ITUD20060280A1 (it) | 2008-06-30 |
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