ES2493166T3 - Haptenos, conjugados de haptenos, composiciones de los mismos y método para su preparación y uso - Google Patents

Haptenos, conjugados de haptenos, composiciones de los mismos y método para su preparación y uso Download PDF

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ES2493166T3
ES2493166T3 ES12151524.1T ES12151524T ES2493166T3 ES 2493166 T3 ES2493166 T3 ES 2493166T3 ES 12151524 T ES12151524 T ES 12151524T ES 2493166 T3 ES2493166 T3 ES 2493166T3
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conjugates
fluorescence microscopy
haptens
haptenos
compositions
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Jerry W. Kosmeder
Mark Lefever
Donald Johnson
Michael Farrell
Zhanna Zhilina
Christopher Bieniarz
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Ventana Medical Systems Inc
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Abstract

Un conjugado que tiene una fórmula (hapteno)m-(conector)n-(grupo reactivo)0-(vehículo)p donde: el hapteno es un mono- o dinitro pirazol; y, m, n, o y p son 1.

Description

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Dos variedades recientemente desarrolladas de DsRed, conocidas como T1 y E57, despliegan una maduración mejorada, lo que hace que resulten preferibles para su uso en experimentos a dos colores. La fluorescencia de algunas variantes de GFP puede ser "fotoactivada" mediante una iluminación específica, que
5 ofrece la ventaja de que la fluorescencia se puede activar en un punto temporal seleccionado. Se han desarrollado tres proteínas fluorescentes que se someten a modificación fotoquímica en el cromóforo o cerca de este, PA-GFP, Kaede y KFP1, que permiten la activación selectiva de señales de fluorescencia tras una iluminación específica y que se pueden utilizar para marcar por fluorescencia células, organelos o proteínas individuales. La Tabla 1 proporciona otros ejemplos de fracciones generadoras de señales y conjugados que comprenden estas
10 fracciones.
Tabla 1 Ejemplos de conjugados de etiqueta detectable de anticuerpo
Etiqueta de conjugado de anticuerpo
Recomendado para Color emitido Excitación de etiqueta Emisión de etiqueta
Lake Placid Blue (EviTag™ Quantum Dot)
Citometría de flujo, inmunoblots y microscopía de fluorescencia <450 490
Fluoresceína (es decir, FITC)
Citometría de flujo, incl. sistemas BD FAC y Guava System, y microscopía de fluorescencia 494 518
Adirondack Green (EviTag™ Quantum Dot)
Citometría de flujo, inmunoblots y microscopía de fluorescencia <450 520
Rhodamine Green
Microscopía de fluorescencia 502 527
Catskill Green (EviTag™ Quantum Dot)
Microscopía de fluorescencia <450 540
Rodamina 6G
Citometría de flujo, inmunoblots y microscopía de fluorescencia 525 555
Hops Yellow (EviTag™ Quantum Dot)
Citometría de flujo, inmunoblots y microscopía de fluorescencia <450 560
Amersham Cy3
Microscopía de fluorescencia 550 565
R-Phycoerythrin (PE)
Citometría de flujo, sistemas Luminex® y Guava, ensayos de FRET, electroforesis capilar y uso con FITC para doble etiquetado (495)565 575
Rhodamine Red
Citometría de flujo, microscopía de fluorescencia 560 580
Birch Yellow (EviTag™ Quantum Dot)
Microscopía de fluorescencia <450 580
Amersham Cy3.5
Microscopía de fluorescencia 581 596
Fort Orange (EviTag™ Quantum Dot)
Citometría de flujo, inmunoblots y microscopía de fluorescencia <450 600
SulfoRhodamine (Alias Texas Red®)
Citometría de flujo y microscopía de fluorescencia 596 615
Amersham Cy5
Immunoblot, incl. Amersham Typhoon System, y aplicaciones inmunofluorescentes 650 670
Allophycocyanin (APC)
Ensayos FRET y ensayos HTRF 652 670
Amershani Cy5.5
Immunoblot, especialmente sistemas LI-COR Odyssey 675 694
Biotina
Citometría de flujo y otras aplicaciones fluorescentes -
15 Muchas de estas etiquetas se pueden utilizar con múltiples anticuerpos que no interreaccionan para crear ensayos multiplexados personalizados.
VII. Procesos para la formación de conjugados de hapteno - Esquemas de reacción
20 Los siguientes esquemas proporcionan ejemplos de realizaciones de un método útil para producir conjugados de la presente invención. Otras metodologías sintéticas también resultan útiles para producir estos conjugados y no se deberá interpretar que los esquemas siguientes limitan el método a las metodologías sintéticas concretas ilustradas.
1. Conjugados de nitropirazol
25
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muestra
debajo NFDM en 0,15m PBS 37C
Ac secundario
Gt-α-Mu-HRP ,15M PBS w/0,05% Tween 20 1 (10000) 50μl 1hr @ 37c
Diseño de la placa
Dilución de la muestra
1:50 1:250 1:1250 1:6250 1:31250 1:156250
1
2 3 4 5 6 7 8 9 10 11 12
Mu#1
A 1,8 9 0,01 1,8 1 0,0 0 1,8 2 0,0 0 1,60 0,00 1,06 0,00 0, 56 0,0 0
Mu#2
B 1,7 6 0,01 1,8 7 0,0 0 1,7 3 0,0 0 1,25 0,00 0,60 0,00 0, 25 0,0 0
Mu#3
C 1,7 0 0,01 1,8 3 0,0 0 1,8 7 0,0 0 1,50 0,00 0,95 0,01 0, 37 0,0 0
Mu#4
D 1,7 5 0,01 1,7 3 0,0 0 1,8 3 0,0 0 1,49 0,01 0,98 0,00 0, 42 0,0 0
Mu#5
E 1,6 2 0,01 1,5 8 0,0 0 1,7 2 0,0 0 1,35 0,00 ,074 0,00 0, 33 0,0 0
Concentrad o premezcla (PbP)
F 0 ,06 0,01 0,0 1 0,0 0 0,0 0 0,0 1 0,00 0,00 0,00 0,00 0, 00 0,0 0
x
x
x
x
x
x
Antígeno
VMSI-1357-98 Lot#C05081610 x = en blanco (sin antígeno)
Solución tampón de lavado
0,15M PBS con 0,05% Tween 20 NFDM = leche en polvo desnatada
Ac secundario:
HRP-cabra-α-Fc de IgG mur específ. min. x-reac. #60312 Fecha de la muestra : 09/12/2005
Sustrato
TMB lote #P502807 Fecha del ensayo: 09/12/2005
Los resultados mostrados en la Tabla 2 indican que cada uno de los ratones sometidos a ensayo es adecuado para desencadenar una respuesta de anticuerpo, y también que estos haptenos se pueden visualizar para confirmar una respuesta. Con respecto al hapteno concreto testado, el ratón número 1 parece proporcionar la mejor respuesta de
5 todas las diluciones testadas.
Ejemplo 28
Este ejemplo se refiere a un ejemplo de procedimiento para conjugar anticuerpos anti-hapteno con peroxidasa de 10 rábano (HRP). Las imágenes producidas utilizando estos conjugados se proporcionan en las Fig. 19-24.
Activación de HRP
A un vial ámbar de 4ml se añadieron 78,8 mg (100 eq.) de éster de MAL-dPEG4TM NHS (Quanta Biodesign, Powell,
15 OH, F.W. = 513.50), seguidos de 2,46 ml (61,5 mg, 1,53 μM) de HRP (Horseradish Peroxidase, Pierce, Rockford, IL Lot FJ925901) en forma de una solución de 25 mg / ml en 0,1 M fosfato sódico, pH 7.5. A continuación, el vial se colocó en un rotador de laboratorio en un lugar oscuro y a temperatura ambiente (23 - 25 ˚C), y se dejó progresar la reacción de formación de conector de amida durante una hora. A continuación, se retiró una parte alícuota de 400 μl para la purificación y el resto de la solución se almacenó temporalmente a 4 ˚C. Se obtuvo maleimida-PEG4-HRP
20 pura fraccionando la muestra en un AKTA Purifier equipado con una columna Superdex 10/300 (GE Lifesciences) y eluyendo con 0,1 M fosfato sódico, pH 7.5 a 1,0 ml / min. La HRP que contenía fracciones se agrupó para obtener 2,0 ml de una solución de 4,52 mg / ml de maleimida-PEG4-HRP (90 % de recuperación), medida mediante
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Tabla 1 Ejemplos de antígenos de interés (antígenos diana)
Antígenos diana virales
Ejemplos de secuencias de antígenos diana de los antígenos diana SEC. ID. Nº:
BK
TLYKKMEQDVKVAHQ GNLPLMRKAYLRKCK TFSRMKYNICMGKCI 1 22 23
JC
SITEVECFL 2
Epstein-Barr (EBV)
QPRAPIRPI 3
citomegalovirus (CMV)
NLVPMVATV 4
HPV
YMLDLQPET(T) 5
Influenza A
GILGFVFTL 6
Antígenos diana del tumor y sus péptidos derivados
PRAME
LYVDSLFFL 7
WT1
RMFPNAPYL 8
Survivin
ELTLGEFLKL 9
AFP
GVALQTMKQ 10
ELF2M
ETVSEQSNV 11
proteinasa 3 y su péptido PR1
VLQELNVTV 12
elastasa de neutrófilos
VLQELNVTV 13
MAGE
EADPTGHSY 14
MART
AAGIGILTV 15
tirosinasa
RHRPLQEVYPEANAPIGHNRE 16
GP100
WNRQLYPEWTEAQRLD 17
NY-Eso-1
VLLKEFTVSG 18
Herceptin
KIFGSLAFL 19
antígeno carcinoembrionario (CEA)
HLFGYSWYK 20
PSA
FLTPKKLQCV 21
Antígeno diana fúngico
Blastomyces dermatitidis
CELDNSHEDYNWNLWFKWCSGHGR TGHGKHFYDCDWDPSHGDYSWYLW DPSHGDYSWYLWDYLCGNGHHPYD DYLCGNGHHPYDCELDNSHEDYSW DPYNCDWDPYHEKEKYDWDLWNKWCN KYDWDLWNKWCNKDPYNCDWDPYH 24 25 26 27 28 29
Tabla 2 Ejemplos de tumores y sus antígenos tumorales
Tumor
Antígenos diana asociados al tumor
Leucemia mielógena aguda
Tumor de Wilms 1 (WT1), preferiblemente antígeno de melanoma expresado (PRAME), PR1, proteinasa 3, elastasa catepsina G
Leucemia mielógena crónica
WT1, PRAME, PR1, proteinasa 3, elastasa, catepsina G
Síndrome mielodisplásico
WT1, PRAME, PR1, proteinasa 3, elastasa, catepsina G
Leucemia linfoblástica aguda
PRAME
Leucemia linfocítica crónica
Superviviente
Linfoma no Hodgkin
Superviviente
Mieloma múltiple
Nueva York esofágico 1 (NY-Eso-1)
Melanoma maligno
MAGE, MART, Tirosinasa, PRAME GP100
Cáncer de mama
WT1, herceptina
Cáncer de pulmón
WT1
Cáncer de próstata
Antígeno prostático específico (PSA)
Cáncer de colon
Antígeno carcinoembriónico (CEA)
Carcinoma de células renales (RCC)
Factor de crecimiento fibroblástico 5 (FGF-5)
Cualquier péptido antigénico (como un fragmento inmunogénico) de un antígeno de interés se puede utilizar para 10 generar una población de linfocitos T específicos para ese antígeno de interés. En la técnica se conocen numerosos
136

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Families Citing this family (96)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002087424A2 (en) 2001-05-02 2002-11-07 Purdue Research Foundation Treatment and diagnosis of macrophage mediated disease
US8043603B2 (en) 2002-02-07 2011-10-25 Endocyte, Inc. Folate targeted enhanced tumor and folate receptor positive tissue optical imaging technology
US8043602B2 (en) 2002-02-07 2011-10-25 Endocyte, Inc. Folate targeted enhanced tumor and folate receptor positive tissue optical imaging technology
JP5175723B2 (ja) 2005-07-05 2013-04-03 パーデュー・リサーチ・ファウンデーション 単球介在性疾患を治療するための組成物の調製
EP1940473A2 (en) 2005-09-23 2008-07-09 Purdue Research Foundation Multiphoton in vivo flow cytometry method and device
WO2007120192A2 (en) * 2005-10-27 2007-10-25 The President And Fellows Of Harvard College Methods and compositions for labeling nucleic acids
CA2858359C (en) 2006-11-01 2018-04-03 Ventana Medical Systems, Inc. Haptens, hapten conjugates, compositions thereof and method for their preparation and use
EP2087337A4 (en) 2006-11-03 2010-09-08 Purdue Research Foundation METHOD AND DEVICE FOR EX-VIVO FLOW CYTOMETRY
AU2008213702B2 (en) 2007-02-07 2014-04-24 Purdue Research Foundation Positron emission tomography imaging method
US7682789B2 (en) * 2007-05-04 2010-03-23 Ventana Medical Systems, Inc. Method for quantifying biomolecules conjugated to a nanoparticle
CA2687178C (en) 2007-05-23 2014-02-04 Ventana Medical Systems, Inc. Polymeric carriers for immunohistochemistry and in situ hybridization
US8961926B2 (en) 2007-05-25 2015-02-24 Purdue Research Foundation Method of imaging localized infections
US9901567B2 (en) 2007-08-01 2018-02-27 Syntarga B.V. Substituted CC-1065 analogs and their conjugates
CA2720728C (en) 2008-06-05 2018-04-03 Ventana Medical Systems, Inc. Compositions comprising nanomaterials and method for using such compositions for histochemical processes
AU2009282760B2 (en) * 2008-08-22 2013-08-15 Ventana Medical Systems, Inc. Method for chromogenic detection of two or more target molecules in a single sample
MX347291B (es) * 2009-03-20 2017-04-17 Amgen Inc Inmunoglobulinas portadoras y usos de las mismas.
EP3483610A1 (en) 2009-11-09 2019-05-15 University Of Washington Center For Commercialization Functionalized chromophoric polymer dots and bioconjugates thereof
USPP22463P3 (en) * 2010-02-16 2012-01-17 Menachem Bornstein Gypsophila plant named ‘Pearl Blossom’
WO2011106583A1 (en) 2010-02-26 2011-09-01 Ventana Medical Systems, Inc. Polytag probes
US10539487B2 (en) 2010-03-04 2020-01-21 Ventana Medical Systems, Inc. Systems and methods for monitoring tissue sample processing
AU2011222501B2 (en) 2010-03-04 2014-05-01 Ventana Medical Systems, Inc. Processing system for processing specimens using acoustic energy
US10126216B2 (en) 2011-02-17 2018-11-13 Ventana Medical Systems, Inc. Method for tissue sample fixation
EP2561095A1 (en) 2010-04-20 2013-02-27 Ventana Medical Systems, Inc. Two-color chromogenic in situ hybridization
KR101901555B1 (ko) 2010-04-21 2018-09-21 신타가 비.브이. Ｃｃ―１０６５ 유사체와 2작용성 링커의 신규한 콘쥬게이트
CA2792569C (en) 2010-04-27 2019-01-15 Ventana Medical Systems, Inc. Antibody-nanoparticle conjugates and methods for making and using such conjugates
DK2588144T3 (en) 2010-07-02 2018-07-23 Ventana Med Syst Inc Detection of targets using mass markers and mass spectrometry
JP2013531801A (ja) * 2010-07-02 2013-08-08 ヴェンタナ メディカル システムズ， インク． 標的検出のためのハプテンコンジュゲート
US8956859B1 (en) 2010-08-13 2015-02-17 Aviex Technologies Llc Compositions and methods for determining successful immunization by one or more vaccines
DK2606055T3 (en) 2010-08-16 2015-09-07 Ventana Med Syst Inc Chromogenic detection substrates and methods for use in detection assays and kits
EA201370076A1 (ru) 2010-09-22 2013-08-30 Амген Инк. Иммуноглобулины-переносчики и их применение
CA2814790C (en) 2010-10-18 2019-11-19 University of Washington Center for Commercialization Chromophoric polymer dots
WO2012068366A2 (en) 2010-11-18 2012-05-24 Yale University Bifunctional molecules with antibody-recruiting and entry inhibitory activity against the human immunodeficiency virus
AU2012228424B2 (en) 2011-03-14 2015-05-07 Ventana Medical Systems, Inc. A method of analyzing chromosomal translocations and a system therefore
US9797840B2 (en) 2011-11-28 2017-10-24 University Of Washington Through Its Center For Commercialization Highly fluorescent polymer nanoparticle
WO2013079606A1 (en) 2011-12-01 2013-06-06 Ventana Medical Systems, Inc. Automated dual stain of mirna and protein targets
US10150841B2 (en) 2011-12-30 2018-12-11 University Of Washington Through Its Center For Commercialization Chromophoric polymer dots with narrow-band emission
WO2013108126A2 (en) 2012-01-16 2013-07-25 University Of Oslo Methyltransferases and uses thereof
WO2013110549A1 (en) 2012-01-23 2013-08-01 Ventana Medical Systems, Inc. Polymer stabilization of chromogen solutions
EP2809510B1 (en) 2012-02-03 2021-03-31 University of Washington through its Center for Commercialization Polyelectrolyte-coated polymer dots and related methods
ES2678211T3 (es) 2012-03-27 2018-08-09 Ventana Medical Systems, Inc. Conjugados de señalización y procedimientos de uso
US9123538B2 (en) * 2012-04-26 2015-09-01 Regents Of The University Of Minnesota Silicon nanocrystal inks, films, and methods
CN102654500A (zh) * 2012-05-17 2012-09-05 重庆市科学技术研究院 一种检测喹噁啉-2-羧酸的检测试剂盒及其方法
WO2014031665A1 (en) * 2012-08-21 2014-02-27 Ortho-Clinical Diagnostics, Inc Antibodies to quetiapine haptens and use thereof
EP2971064B1 (en) 2013-03-12 2019-10-16 Ventana Medical Systems, Inc. Proximity assay for in situ detection of targets
WO2014139979A1 (en) 2013-03-12 2014-09-18 Ventana Medical Systems, Inc. Quantum dot in situ hybridization
CA2900842C (en) 2013-03-12 2020-01-21 Ventana Medical Systems, Inc. Digitally enhanced microscopy for multiplexed histology
JP6587605B2 (ja) 2013-03-14 2019-10-09 ユニバーシティ オブ ワシントン スルー イッツ センター フォー コマーシャリゼーション ポリマードット組成物および関連方法
DK3063291T3 (da) * 2013-10-11 2019-05-06 Ventana Med Syst Inc Multiplex her2 og østrogenreceptor co-farvnings-assays til påvisning af tumorheterogenitet
ES2742308T3 (es) 2013-10-24 2020-02-13 Siemens Healthcare Diagnostics Inc Ensayos de analitos macromoleculares
MY189713A (en) 2014-01-10 2022-02-28 Univ Yale Duocarmycin adcs for use in treatment of endometrial cancer
EP3069735B1 (en) 2014-01-10 2018-03-14 Synthon Biopharmaceuticals B.V. Duocarmycin adcs for use in the treatment of bladder cancer
EP3110966A1 (en) 2014-02-24 2017-01-04 Ventana Medical Systems, Inc. Automated rna detection using labeled 2'-o-methyl rna oligonucleotide probes and signal amplification systems
AU2015220750B2 (en) 2014-02-24 2020-04-16 Ventana Medical Systems, Inc. Quinone methide analog signal amplification
DK3152577T3 (en) 2014-06-06 2018-10-01 Ventana Med Syst Inc SIGNIFICANCE OF INTRATUMORAL HER2 HETEROGENITY IN BREAST CANCER AND APPLICATIONS HEFAR
WO2016046173A1 (en) * 2014-09-22 2016-03-31 Synthon Biopharmaceuticals B.V. Pan-reactive antibodies to duocarmycins
CA2957783C (en) * 2014-10-02 2023-02-28 Ventana Medical Systems, Inc. Polymers and conjugates comprising the same
WO2016083364A1 (en) 2014-11-25 2016-06-02 Ventana Medical Systems, Inc. Proximity assays using chemical ligation and hapten transfer
CN107209934B (zh) 2014-12-03 2021-03-30 文塔纳医疗系统公司 用于定量分析异质生物标志物分布的方法、系统和装置
CA2976005A1 (en) 2015-02-06 2016-08-11 Cell Idx, Inc. Antigen-coupled immunoreagents
WO2016166073A1 (en) 2015-04-13 2016-10-20 Ventana Medical Systems, Inc. Thermochemical-based antibody inactivation methods and systems
EP3283522A1 (en) 2015-04-17 2018-02-21 Spring Bioscience Corporation Antibodies, compositions, and immunohistochemistry methods for detecting c4.4a
WO2016190236A1 (ja) * 2015-05-28 2016-12-01 コニカミノルタ株式会社 インサイチュハイブリダイゼイション用のプローブ試薬
CN105018072B (zh) * 2015-07-08 2017-02-01 山东理工大学 检测人血清白蛋白的荧光探针及其制备方法
JP6880001B2 (ja) 2015-08-28 2021-06-02 ヴェンタナ メディカル システムズ， インク． ケージドハプテンを使用するホルマリン固定パラフィン包埋組織におけるタンパク質近接アッセイ
KR20180081561A (ko) 2015-11-06 2018-07-16 벤타나 메디컬 시스템즈, 인코포레이티드 대표 진단법
AU2017229370B2 (en) 2016-03-08 2021-03-11 Ventana Medical Systems, Inc. Multiplexed immunohistochemistry using recombinant antibodies with epitope tags
EP3475701B1 (en) 2016-06-28 2023-02-22 Ventana Medical Systems, Inc. New colors for chromogenic ihc and ish staining with multi-dye quinone methide and tyramide conjugates
EP3475291A1 (en) 2016-06-28 2019-05-01 Ventana Medical Systems, Inc. Application of click chemistry for signal amplification in ihc and ish assays
CN109716130B (zh) * 2016-07-18 2023-05-23 赛尔伊迪克斯公司 抗原偶联的杂交试剂
JP7203018B2 (ja) * 2016-09-26 2023-01-12 デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド クロモボックスタンパク質阻害剤およびその使用
DK3929304T3 (da) * 2016-12-16 2024-04-08 Aratome Llc Molekylær detektering ved ligeringsforstærkning
EP3554556B1 (en) 2016-12-19 2022-03-09 Ventana Medical Systems, Inc. Peptide nucleic acid conjugates
WO2018118786A1 (en) 2016-12-19 2018-06-28 Ventana Medical Systems, Inc. Methods and systems for quantitative immunohistochemistry
EP3568157A4 (en) * 2017-01-13 2021-01-06 National Research Council of Canada METHOD FOR OPTIMIZING THE PEPTIDE IMMUNO-EPITOP BY GLYCOSYLATION, OPTIMIZED PEPTIDE THEREOF AND ITS USE FOR CONJUGATE VACCINES
CN117551741A (zh) 2017-03-31 2024-02-13 乌尔蒂维尤股份有限公司 Dna-抗原交换和扩增
US10768185B2 (en) * 2017-07-20 2020-09-08 Trustees Of Boston University Tenofovir detection assay
EP3727470A1 (en) 2017-12-18 2020-10-28 Ventana Medical Systems, Inc. Peptide nucleic acid conjugates
US10330684B1 (en) 2018-04-05 2019-06-25 Rarecyte, Inc. Analyte detection
WO2019194994A1 (en) * 2018-04-05 2019-10-10 Rarecyte, Inc. Analyte detection
WO2019224153A1 (en) 2018-05-21 2019-11-28 Genentech, Inc. Her2 heterogeneity as a biomarker in cancer
WO2020058339A1 (en) * 2018-09-20 2020-03-26 Ventana Medical Systems, Inc. Coumarin-based crosslinking reagents
WO2020229578A1 (en) 2019-05-14 2020-11-19 Ventana Medical Systems, Inc. System including a biological sample treatment chamber
CN110330488A (zh) * 2019-06-20 2019-10-15 深圳市易瑞生物技术股份有限公司 一种吡罗昔康半抗原及其制备方法和应用
WO2021123074A1 (en) 2019-12-18 2021-06-24 F. Hoffmann-La Roche Ag Methods of sequencing by synthesis using a consecutive labeling scheme
US11814677B2 (en) * 2019-12-30 2023-11-14 Arizona Board Of Regents On Behalf Of Arizona State University Methods and systems for sensitive and multiplexed analysis of biological samples using cleavable fluorescent streptavidin and anti-hapten antibodies
WO2021173674A1 (en) 2020-02-26 2021-09-02 A2 Biotherapeutics, Inc. Polypeptides targeting mage-a3 peptide-mhc complexes and methods of use thereof
CN115552248A (zh) 2020-05-07 2022-12-30 文塔纳医疗系统公司 用于评价肿瘤样品中egfr和egfr配体表达的组织化学系统和方法
KR102506288B1 (ko) * 2020-09-07 2023-03-06 국립암센터 디그옥시제닌에 대한 항체를 포함하는 복합체, 및 이들의 용도
CN112362863B (zh) * 2020-11-02 2024-02-13 上海市农业科学院 一种巯基功能化脂质体纳米材料及其制备方法和应用
EP4278181A1 (en) 2021-01-15 2023-11-22 Ventana Medical Systems, Inc. Storage stable caged haptens
EP4281779A1 (en) 2021-01-25 2023-11-29 Ventana Medical Systems, Inc. Stained biological specimens including one or more biomarkers labeled with one or more detectable moieties
JP2024516380A (ja) 2021-04-18 2024-04-15 ヴェンタナ メディカル システムズ， インク． 形態学的マーカー染色
WO2022240641A1 (en) * 2021-05-12 2022-11-17 The Scripps Research Institute High-throughput assay for identification of myc inhibitors
WO2023205669A2 (en) * 2022-04-19 2023-10-26 Purdue Research Foundation Dual and triple hapten conjugates, compositions, processes for making, and methods of treatment therewith
WO2023232829A1 (en) * 2022-05-31 2023-12-07 Illumina, Inc Compositions and methods for nucleic acid sequencing
WO2024002924A2 (en) 2022-06-28 2024-01-04 F. Hoffmann-La Roche Ag Fluorescent dyes with large stokes shift

Family Cites Families (170)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3892530A (en) 1974-09-23 1975-07-01 Hoffmann La Roche Colorimetric and fluorometric method
US4036945A (en) 1976-05-03 1977-07-19 The Massachusetts General Hospital Composition and method for determining the size and location of myocardial infarcts
US4230683A (en) * 1978-08-09 1980-10-28 Abbott Laboratories Hapten conjugated antibody for antibody or antigen detection
US4223013A (en) * 1978-12-29 1980-09-16 Syva Company Amitriptyline conjugates to antigenic proteins and enzymes
US4495296A (en) * 1979-05-21 1985-01-22 New York Blood Center, Inc. Labeled anti-hapten antibodies and their use as a universal reagent for solid phase radio- and/or enzyme-immunoassays
US4331647A (en) 1980-03-03 1982-05-25 Goldenberg Milton David Tumor localization and therapy with labeled antibody fragments specific to tumor-associated markers
US4425427A (en) 1980-03-13 1984-01-10 Vitafin N.V. Simultaneous, kinetic, spectrophotometric analysis of blood serum for multiple components
US4675189A (en) 1980-11-18 1987-06-23 Syntex (U.S.A.) Inc. Microencapsulation of water soluble active polypeptides
US4469797A (en) 1982-09-23 1984-09-04 Miles Laboratories, Inc. Digoxigenin immunogens, antibodies, labeled conjugates, and related derivatives
US4490473A (en) * 1983-03-28 1984-12-25 Panab Labeled antibodies and methods
JPS60100516A (ja) 1983-11-04 1985-06-04 Takeda Chem Ind Ltd 徐放型マイクロカプセルの製造法
CA1256638A (en) 1984-07-06 1989-06-27 Motoaki Tanaka Polymer and its production
US4879224A (en) 1985-01-10 1989-11-07 Biogen, Inc. DNA sequences, recombinant DNA molecules and processes for producing human phospholipase inhibitor polypeptides
JP2551756B2 (ja) 1985-05-07 1996-11-06 武田薬品工業株式会社 ポリオキシカルボン酸エステルおよびその製造法
US6280929B1 (en) 1986-01-16 2001-08-28 The Regents Of The University Of California Method of detecting genetic translocations identified with chromosomal abnormalities
US5756696A (en) 1986-01-16 1998-05-26 Regents Of The University Of California Compositions for chromosome-specific staining
US5447841A (en) 1986-01-16 1995-09-05 The Regents Of The Univ. Of California Methods for chromosome-specific staining
US4946778A (en) 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
ES2039025T3 (es) 1987-11-16 1993-08-16 Janssen Pharmaceutica N.V. Un agregado para determinar sustancias combinables.
DE3811692A1 (de) * 1988-04-07 1989-10-19 Idt Ag Fuer In Vivo Diagnostik Diagnostikverfahren und mittel zur diagnose und behandlung immunologischer erkrankungen
CA1308022C (en) * 1988-08-11 1992-09-29 Eleftherios P. Diamandis Multiple fluorescence labelling with europium chelators
DE3836656A1 (de) 1988-10-27 1990-05-03 Boehringer Mannheim Gmbh Neue digoxigenin-derivate und ihre verwendung
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5595707A (en) 1990-03-02 1997-01-21 Ventana Medical Systems, Inc. Automated biological reaction apparatus
US5225325A (en) 1990-03-02 1993-07-06 Ventana Medical Systems, Inc. Immunohistochemical staining method and reagents therefor
WO1992016838A1 (en) * 1991-03-20 1992-10-01 Gec-Marconi Limited Separation method
WO1993020094A1 (en) * 1992-03-27 1993-10-14 Abbott Laboratories Haptens, tracers, immunogens and antibodies for quinoline
US6100099A (en) * 1994-09-06 2000-08-08 Abbott Laboratories Test strip having a diagonal array of capture spots
US5403747A (en) 1992-04-01 1995-04-04 Thomas Jefferson University Protein assay using microwave energy
US5350686A (en) 1992-04-16 1994-09-27 Pioneer Hi-Bred International, Inc. Microwave acceleration of enzyme-catalyzed modification of macromolecules
US5834202A (en) 1992-08-04 1998-11-10 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US6005079A (en) 1992-08-21 1999-12-21 Vrije Universiteit Brussels Immunoglobulins devoid of light chains
DK0656946T4 (da) 1992-08-21 2010-07-26 Univ Bruxelles Immunoglobuliner uden lette kæder
US5393891A (en) * 1993-06-08 1995-02-28 The Du Pont Merck Pharmaceutical Company Immunoassay reagents and methods for detecting brequinar and analogs
US5679582A (en) 1993-06-21 1997-10-21 Scriptgen Pharmaceuticals, Inc. Screening method for identifying ligands for target proteins
US5661040A (en) 1993-07-13 1997-08-26 Abbott Laboratories Fluorescent polymer labeled conjugates and intermediates
EP0708767B1 (en) * 1993-07-20 2001-02-14 Abbott Laboratories Haptens, tracers, immunogens and antibodies for carbazole and dibenzofuran derivatives
US5731171A (en) 1993-07-23 1998-03-24 Arch Development Corp. Sequence independent amplification of DNA
US5643761A (en) 1993-10-27 1997-07-01 The Trustees Of Columbia University In The City Of New York Method for generating a subtracted cDNA library and uses of the generated library
US5487975A (en) 1993-11-15 1996-01-30 Ventana Medical Systems, Inc. Biotin/avidin formulation
DE4407538C1 (de) * 1994-03-07 1995-02-23 Deutsches Krebsforsch Bindungsreagens für Zell-Oberflächenprotein und Effektorzelle
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US6207646B1 (en) 1994-07-15 2001-03-27 University Of Iowa Research Foundation Immunostimulatory nucleic acid molecules
US6239116B1 (en) 1994-07-15 2001-05-29 University Of Iowa Research Foundation Immunostimulatory nucleic acid molecules
US6429199B1 (en) 1994-07-15 2002-08-06 University Of Iowa Research Foundation Immunostimulatory nucleic acid molecules for activating dendritic cells
ATE420171T1 (de) 1994-07-15 2009-01-15 Univ Iowa Res Found Immunomodulatorische oligonukleotide
GB9420355D0 (en) 1994-10-10 1994-11-23 Univ Nottingham Preparation of protein microspheres, films and coatings
US6057099A (en) 1994-12-02 2000-05-02 Intelligene Ltd. Detection of nucleic acid sequences
US5830645A (en) 1994-12-09 1998-11-03 The Regents Of The University Of California Comparative fluorescence hybridization to nucleic acid arrays
US5648245A (en) 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US5684142A (en) 1995-06-07 1997-11-04 Oncor, Inc. Modified nucleotides for nucleic acid labeling
US5883081A (en) 1995-07-26 1999-03-16 The Regents Of The University Of California Isolation of novel HIV-2 proviruses
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US6750016B2 (en) 1996-07-29 2004-06-15 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6506564B1 (en) 1996-07-29 2003-01-14 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6361944B1 (en) 1996-07-29 2002-03-26 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
US6582921B2 (en) 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
US6767702B2 (en) 1996-07-29 2004-07-27 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses therefor
JPH10104230A (ja) * 1996-09-30 1998-04-24 Aisin Seiki Co Ltd 核酸等の検出方法，並びに標識物質及び検出物質
EP0951566B1 (en) 1996-10-04 2002-12-18 Universität Heidelberg A method of purifying dna
US5952890A (en) 1997-02-05 1999-09-14 Fox Enterprises, Inc. Crystal oscillator programmable with frequency-defining parameters
WO1998037919A1 (en) 1997-02-28 1998-09-03 University Of Iowa Research Foundation USE OF NUCLEIC ACIDS CONTAINING UNMETHYLATED CpG DINUCLEOTIDE IN THE TREATMENT OF LPS-ASSOCIATED DISORDERS
US6406705B1 (en) 1997-03-10 2002-06-18 University Of Iowa Research Foundation Use of nucleic acids containing unmethylated CpG dinucleotide as an adjuvant
US5994071A (en) 1997-04-04 1999-11-30 Albany Medical College Assessment of prostate cancer
EP1003531B1 (en) 1997-05-20 2007-08-22 Ottawa Health Research Institute Processes for preparing nucleic acid constructs
JPH1151935A (ja) * 1997-08-06 1999-02-26 Aisin Seiki Co Ltd 複数種類のターゲット分子の同時検出方法
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
US6322901B1 (en) 1997-11-13 2001-11-27 Massachusetts Institute Of Technology Highly luminescent color-selective nano-crystalline materials
JPH11240879A (ja) * 1997-12-22 1999-09-07 Nissan Chem Ind Ltd ピラゾールスルホニルウレアのポリクローナル抗体及び検出方法
US6358682B1 (en) 1998-01-26 2002-03-19 Ventana Medical Systems, Inc. Method and kit for the prognostication of breast cancer
US6296809B1 (en) 1998-02-27 2001-10-02 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US20030211630A1 (en) 1998-02-27 2003-11-13 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US6855559B1 (en) 1998-09-03 2005-02-15 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
US6582962B1 (en) 1998-02-27 2003-06-24 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
AU760549B2 (en) 1998-04-03 2003-05-15 University Of Iowa Research Foundation, The Methods and products for stimulating the immune system using immunotherapeutic oligonucleotides and cytokines
US7550298B2 (en) 1998-09-03 2009-06-23 Ventana Medical Systems, Inc. Automated immunohistochemical and in situ hybridization assay formulations
US6855552B2 (en) 1998-09-03 2005-02-15 Ventana Medical Systems Automated immunohistochemical and in situ hybridization assay formulations
JP2000095769A (ja) * 1998-09-22 2000-04-04 Kankyo Meneki Gijutsu Kenkyusho:Kk プロベナゾールのハプテン化合物、抗体及び測定方法
JP4630459B2 (ja) 1998-09-24 2011-02-09 インディアナ・ユニバーシティ・リサーチ・アンド・テクノロジー・コーポレーション 水溶性発光量子ドットおよびその生体分子コンジュゲート
US6414133B1 (en) 1998-10-13 2002-07-02 Ventana Medical Systems, Inc. Multiple fusion probes
US7569344B2 (en) 1998-10-26 2009-08-04 Ventana Medical Systems, Inc. Detection of human papilloma virus in papanicolaou (Pap) smears
US6432320B1 (en) 1998-11-02 2002-08-13 Patrick Bonsignore Refrigerant and heat transfer fluid additive
US6699973B1 (en) 1998-11-19 2004-03-02 Elan Corporation, Plc Antibodies to peptides that target GIT receptors and related methods
JP2003526328A (ja) 1999-01-11 2003-09-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Ｄｎａの等温増幅
US6344337B1 (en) 1999-02-19 2002-02-05 Board Of Trustees Of Michigan State University Antigen test to detect equine protozoal myeloencephalitis in horse serum and cerebrospinal fluid
US6696304B1 (en) 1999-02-24 2004-02-24 Luminex Corporation Particulate solid phase immobilized protein quantitation
US6544798B1 (en) 1999-02-26 2003-04-08 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
US6673214B1 (en) 1999-04-09 2004-01-06 Rocky Mountain Biosystems, Inc. Energy enhanced reaction catalysis and uses thereof
WO2000062071A1 (de) 1999-04-13 2000-10-19 Wilex Ag Diagnostischer und therapeutischer einsatz von antikörpern gegen den urokinase-rezeptor
CA2373146A1 (en) * 1999-05-07 2000-11-16 Quantum Dot Corporation A method of detecting an analyte using semiconductor nanocrystals
US6180349B1 (en) 1999-05-18 2001-01-30 The Regents Of The University Of California Quantitative PCR method to enumerate DNA copy number
US6326148B1 (en) 1999-07-12 2001-12-04 The Regents Of The University Of California Detection of copy number changes in colon cancer
DE19933701A1 (de) 1999-07-19 2001-01-25 Wilex Biotechnology Gmbh Zyklische peptidomimetische Urokinaserezeptorantagonisten
JP2001089484A (ja) * 1999-09-22 2001-04-03 Kankyo Meneki Gijutsu Kenkyusho:Kk トリシクラゾールのハプテン化合物、抗体及び測定方法
EP1218547A4 (en) * 1999-10-15 2005-04-20 Ventana Med Syst Inc METHOD FOR DETECTING UNIQUE GENE IN SITU COPIES
US20060275784A1 (en) 1999-10-26 2006-12-07 Ventana Medical Systems, Inc. Detection of Human Papilloma Virus in Papanicolaou (Pap) Smears
JP2003519796A (ja) 2000-01-12 2003-06-24 ヴェンタナ メディカル システムズ インコーポレイテッド 画像分析によるタンパク質の定量法
DE60128458T2 (de) 2000-03-20 2008-01-10 Massachusetts Institute Of Technology, Cambridge Anorganische teilchenkonjugate
AU2001253529B2 (en) 2000-04-14 2006-04-27 Board Of Trustees Of The University Of Illinois Method for quantification of akt protein expression
US6291187B1 (en) 2000-05-12 2001-09-18 Molecular Staging, Inc. Poly-primed amplification of nucleic acid sequences
US6828097B1 (en) 2000-05-16 2004-12-07 The Childrens Mercy Hospital Single copy genomic hybridization probes and method of generating same
NZ539223A (en) 2000-05-16 2006-10-27 Childrens Mercy Hospital Single copy genomic hybridization probes and method of generating same
US6794362B1 (en) 2000-05-30 2004-09-21 Connective Tissue Imagineering Llc Asparagine containing elastin peptide analogs
US7763421B2 (en) 2000-06-05 2010-07-27 Ventana Medical Systems, Inc. Methods for producing nucleic acid hybridization probes that amplify hybridization signal by promoting network formation
US6323009B1 (en) 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
US6447692B1 (en) 2000-08-04 2002-09-10 Hrl Laboratories, Llc Nanometer sized phase change materials for enhanced heat transfer fluid performance
US6942970B2 (en) 2000-09-14 2005-09-13 Zymed Laboratories, Inc. Identifying subjects suitable for topoisomerase II inhibitor treatment
US20040209303A1 (en) 2000-10-03 2004-10-21 Martin Mark T. Methods and compositions for directed microwave chemistry
US6649138B2 (en) 2000-10-13 2003-11-18 Quantum Dot Corporation Surface-modified semiconductive and metallic nanoparticles having enhanced dispersibility in aqueous media
US6576291B2 (en) 2000-12-08 2003-06-10 Massachusetts Institute Of Technology Preparation of nanocrystallites
JP4583710B2 (ja) 2000-12-11 2010-11-17 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ ナノセンサ
US20020083888A1 (en) 2000-12-28 2002-07-04 Zehnder Donald A. Flow synthesis of quantum dot nanocrystals
US20030022166A1 (en) 2001-01-19 2003-01-30 Colin Collins Repeat-free probes for molecular cytogenetics
US6656685B2 (en) 2001-01-29 2003-12-02 Ventana Medical Systems, Inc. Hybridization buffers using low molecular weight dextran sulfate and methods for their use
CA2436218A1 (en) 2001-01-30 2003-01-16 Materials And Electrochemical Research (Mer) Corporation Nano carbon materials for enhancing thermal transfer in fluids
DE10105912A1 (de) 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Rekombinante Proteinase K
US20030003537A1 (en) 2001-04-30 2003-01-02 Ventana Medical Systems, Inc. Method and composition of matter for enhancing staining of microorganisms
CA2445881C (en) 2001-04-30 2010-06-22 Ventana Medical Systems, Inc. Reagents and methods for automated hybridization
NZ511705A (en) * 2001-05-14 2004-03-26 Horticulture & Food Res Inst Methods and rapid immunoassay device for detecting progesterone and other steroids
US20030022243A1 (en) 2001-06-20 2003-01-30 Les Kondejewski Protein aggregation assays and uses thereof
EP1402263B1 (en) * 2001-07-02 2009-10-28 Arctic Diagnostics OY Two-photon absorbing dipyrrometheneboron difluoride dyes and their applications
CA2453450A1 (en) 2001-07-20 2003-11-06 Quantum Dot Corporation Luminescent nanoparticles and methods for their preparation
US20030066998A1 (en) 2001-08-02 2003-04-10 Lee Howard Wing Hoon Quantum dots of Group IV semiconductor materials
WO2003031419A1 (en) * 2001-10-09 2003-04-17 3-Dimensional Pharmaceuticals, Inc. Substituted diphenyloxazoles, the synthesis thereof, and the use thereof as fluorescence probes
US6617137B2 (en) 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US6977148B2 (en) 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US20030165485A1 (en) 2001-11-09 2003-09-04 Goran Bertilsson Functional role and potential therapeutic use of Reelin, Gas6 and Protein S in relation to adult neural stem or progenitor cells
WO2003048317A2 (en) 2001-11-30 2003-06-12 Children's Hospital Medical Center Antibodies to magmas and uses thereof
US20050019901A1 (en) 2002-01-31 2005-01-27 Evgenia Matveeva Methods for synthesis of bio-active nanoparticles and nanocapsules for use in optical bio-disc assays and disc assembly including same
US20040248151A1 (en) 2002-04-05 2004-12-09 Ventana Medical Systems, Inc. Method for predicting the response to HER2-directed therapy
US7285289B2 (en) 2002-04-12 2007-10-23 Nagy Jon O Nanoparticle vaccines
US20040057958A1 (en) 2002-05-17 2004-03-25 Waggoner David W. Immunogenicity-enhancing carriers and compositions thereof and methods of using the same
US7476442B2 (en) 2002-07-17 2009-01-13 Massachusetts Institute Of Technology Nanoparticle chains and preparation thereof
US20060194715A1 (en) 2002-10-25 2006-08-31 Blackburn Jonathan M Proteins encoded by ble genes and antibiotics from the bleomycin family
US7200252B2 (en) 2002-10-28 2007-04-03 Ventana Medical Systems, Inc. Color space transformations for use in identifying objects of interest in biological specimens
US7233817B2 (en) 2002-11-01 2007-06-19 Brian Yen Apparatus and method for pattern delivery of radiation and biological characteristic analysis
US20040101822A1 (en) 2002-11-26 2004-05-27 Ulrich Wiesner Fluorescent silica-based nanoparticles
GB0227738D0 (en) 2002-11-28 2003-01-08 Univ Liverpool Nanoparticle conjugates and method of production thereof
US20040115727A1 (en) 2002-12-11 2004-06-17 Allergan, Inc., A Corporation Evolved clostridial toxins with altered protease specificity
US7056471B1 (en) 2002-12-16 2006-06-06 Agency For Science Technology & Research Ternary and quarternary nanocrystals, processes for their production and uses thereof
US20040229300A1 (en) 2003-04-22 2004-11-18 Frederickson Christopher J. Zinc-based screening test and kit for early diagnosis of prostate cancer
US6944333B2 (en) 2003-04-30 2005-09-13 Ventana Medical Systems, Inc. Color image compression via spectral decorrelation and elimination of spatial redundancy
US7981667B2 (en) 2003-05-07 2011-07-19 Indiana University Research And Technology Corporation Alloyed semiconductor quantum dots and concentration-gradient alloyed quantum dots, series comprising the same and methods related thereto
EP1625382A2 (en) 2003-05-19 2006-02-15 Ventana Medical Systems, Inc. Electrophoretic in situ tissue staining
US20050181394A1 (en) 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
DK1636586T3 (da) 2003-06-24 2009-11-23 Ventana Med Syst Inc Enzymkatalyseret metaldeponering til foröget in situ-detektion af immunhistokemisker epitoper og nucleinsyresekvenser
KR100657891B1 (ko) 2003-07-19 2006-12-14 삼성전자주식회사 반도체 나노결정 및 그 제조방법
US20050048498A1 (en) 2003-08-29 2005-03-03 Applera Corporation Compositions, methods, and kits for assembling probes
KR100571817B1 (ko) 2003-09-19 2006-04-17 삼성전자주식회사 태그 서열에 혼성화하는 검출 프로브를 이용하는 표적핵산의 검출방법
EP1687445A4 (en) 2003-09-23 2007-03-28 Atom Sciences Inc POLYMERIC NUCLEIC ACID HYBRIDIZATION SUBSTANCES
CN1886426A (zh) 2003-11-07 2006-12-27 伊姆尼斯公司 结合白细胞介素－4受体的抗体
TW200526224A (en) 2003-12-22 2005-08-16 Alcon Inc Short form c-Maf transcription factor antagonists for treatment of glaucoma
WO2005064018A2 (en) * 2003-12-22 2005-07-14 Ventana Medical Systems, Inc. Microwave mediated synthesis of nucleic acid probes
US7104313B2 (en) 2003-12-31 2006-09-12 Intel Corporation Apparatus for using fluid laden with nanoparticles for application in electronic cooling
US7244665B2 (en) 2004-04-29 2007-07-17 Micron Technology, Inc. Wafer edge ring structures and methods of formation
US20050272032A1 (en) 2004-05-04 2005-12-08 Ventana Medical Systems, Inc., A Corporation Of The State Of Delaware Internal control for in situ hybridization
US20060110744A1 (en) 2004-11-23 2006-05-25 Sampas Nicolas M Probe design methods and microarrays for comparative genomic hybridization and location analysis
US7947839B2 (en) * 2004-12-01 2011-05-24 Genentech, Inc. Heterocyclic-substituted bis-1,8 naphthalimide compounds, antibody drug conjugates, and methods of use
US20060160116A1 (en) 2004-12-16 2006-07-20 The Regents Of The University Of California Repetitive sequence-free DNA libraries
US20060246524A1 (en) 2005-04-28 2006-11-02 Christina Bauer Nanoparticle conjugates
US7615800B2 (en) 2005-09-14 2009-11-10 Eastman Kodak Company Quantum dot light emitting layer
ES2548518T3 (es) * 2005-11-23 2015-10-19 Ventana Medical Systems, Inc. Conjugado molecular
CA2634896C (en) 2005-12-30 2018-08-07 Ventana Medical Systems, Inc. Na+, k+-atpase expression in cervical dysplasia and cancer
WO2008028156A2 (en) 2006-09-01 2008-03-06 Ventana Medical Systems, Inc. Method for producing nucleic acid probes
CA2858359C (en) 2006-11-01 2018-04-03 Ventana Medical Systems, Inc. Haptens, hapten conjugates, compositions thereof and method for their preparation and use
US7682789B2 (en) 2007-05-04 2010-03-23 Ventana Medical Systems, Inc. Method for quantifying biomolecules conjugated to a nanoparticle
CA2687178C (en) * 2007-05-23 2014-02-04 Ventana Medical Systems, Inc. Polymeric carriers for immunohistochemistry and in situ hybridization
WO2010104899A1 (en) * 2009-03-12 2010-09-16 Glaxosmithkline Llc Thiazole sulfonamide and oxazole sulfonamide kinase inhibitors

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