ES2336946T3 - Metodo de diagnostico para trastornos relacionados con daño cerebral. - Google Patents
Metodo de diagnostico para trastornos relacionados con daño cerebral. Download PDFInfo
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- ES2336946T3 ES2336946T3 ES06765356T ES06765356T ES2336946T3 ES 2336946 T3 ES2336946 T3 ES 2336946T3 ES 06765356 T ES06765356 T ES 06765356T ES 06765356 T ES06765356 T ES 06765356T ES 2336946 T3 ES2336946 T3 ES 2336946T3
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| US8492107B2 (en) | 2004-04-15 | 2013-07-23 | University Of Florida Research Foundation, Inc. | Neural proteins as biomarkers for nervous system injury and other neural disorders |
| DK2021792T3 (da) * | 2006-05-09 | 2013-05-21 | Univ British Columbia | Opløste protein-artritmarkører |
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| US9079947B2 (en) | 2007-11-27 | 2015-07-14 | The University Of British Columbia | 14-3-3 eta antibodies and uses thereof for the diagnosis and treatment of arthritis |
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| US20120003225A1 (en) * | 2008-05-09 | 2012-01-05 | Duke University | Autoantibodies in the detection and treatment of cancer |
| ES2665245T3 (es) | 2008-08-11 | 2018-04-25 | Banyan Biomarkers, Inc. | Proceso de detección de biomarcador y ensayo de estado neurológico |
| US12601749B2 (en) | 2008-08-11 | 2026-04-14 | Banyan Biomarkers, Inc. | Biomarker detection process and assay of neurological condition |
| JP5755149B2 (ja) * | 2009-01-26 | 2015-07-29 | エレクトロフォレティクス リミテッドＥｌｅｃｔｒｏｐｈｏｒｅｔｉｃｓ Ｌｉｍｉｔｅｄ | 方法 |
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| EP2462444B1 (en) * | 2009-08-07 | 2018-10-03 | Affinimark Technologies, Inc. | Methods for the immunological identification of cerebrospinal fluid |
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| RU2422095C1 (ru) * | 2009-12-11 | 2011-06-27 | Федеральное государственное учреждение "Ростовский научно-исследовательский онкологический институт Федерального агентства по высокотехнологичной медицинской помощи" | Способ прогнозирования эффективности местной химиотерапии при лечении больных злокачественными опухолями головного мозга |
| WO2011106322A2 (en) * | 2010-02-23 | 2011-09-01 | The Govt. Of The U.S.A. As Represented By The Secretary, Department Of Health And Human Services. | Biomarkers for acute ischemic stroke |
| GB201008541D0 (en) * | 2010-05-21 | 2010-07-07 | Univ Geneve | Diagnostic methods |
| EP2596134B1 (en) | 2010-07-23 | 2020-04-08 | President and Fellows of Harvard College | Methods of detecting diseases or conditions using phagocytic cells |
| WO2012012693A2 (en) | 2010-07-23 | 2012-01-26 | President And Fellows Of Harvard College | Methods for detecting signatures of disease or conditions in bodily fluids |
| CA2806304A1 (en) | 2010-07-23 | 2012-01-26 | President And Fellows Of Harvard College | Methods of detecting prenatal or pregnancy-related diseases or conditions |
| AU2011280997A1 (en) | 2010-07-23 | 2013-02-28 | President And Fellows Of Harvard College | Methods of detecting autoimmune or immune-related diseases or conditions |
| EP2628013B1 (en) | 2010-10-14 | 2019-06-12 | The Johns Hopkins University | Biomarkers of brain injury |
| WO2012081701A1 (ja) | 2010-12-16 | 2012-06-21 | 独立行政法人産業技術総合研究所 | 髄液型糖タンパク質の富化及び分離方法、並びにその方法を用いた中枢神経系疾患用マーカーの探索方法及び中枢神経系疾患用マーカー |
| WO2012152970A1 (es) * | 2011-05-06 | 2012-11-15 | Fundació Institut D'investigació En Ciències De La Salut Germans Trias I Pujol | Método de diagnóstico del ictus isquémico |
| JP2014518624A (ja) | 2011-05-12 | 2014-08-07 | ザ・ジョンズ・ホプキンス・ユニバーシティー | ニューログラニン診断キットのためのアッセイ試薬 |
| EP2758781A4 (en) | 2011-09-22 | 2015-05-27 | Bio Rad Laboratories | SYSTEMS AND METHOD FOR BIOCHEMICAL DATA ANALYSIS |
| AU2012325604B2 (en) | 2011-10-21 | 2017-08-03 | Augurex Life Sciences Corp. | Antigens derived from citrullinated 14-3-3 and uses thereof in the diagnosis of rheumatoid arthritis |
| WO2013090285A1 (en) * | 2011-12-14 | 2013-06-20 | University Of Rochester | Method of diagnosing mild traumatic brain injury |
| US9709573B2 (en) | 2012-03-13 | 2017-07-18 | The Johns Hopkins University | Citrullinated brain and neurological proteins as biomarkers of brain injury or neurodegeneration |
| EP2841945A4 (en) * | 2012-04-24 | 2016-04-27 | Astute Medical Inc | METHOD AND COMPOSITIONS FOR DIAGNOSIS AND FORECASTING OF STROKE OR OTHER BRAINS |
| EP2861788B1 (en) | 2012-06-15 | 2018-10-10 | Progenity, Inc. | Methods of detecting diseases or conditions using circulating diseased cells |
| WO2013188846A1 (en) | 2012-06-15 | 2013-12-19 | Harry Stylli | Methods of detecting diseases or conditions |
| US11585814B2 (en) | 2013-03-09 | 2023-02-21 | Immunis.Ai, Inc. | Methods of detecting prostate cancer |
| EP2965077B1 (en) | 2013-03-09 | 2022-07-13 | Harry Stylli | Methods of detecting cancer |
| CN111426848A (zh) * | 2013-06-28 | 2020-07-17 | 株式会社 Mcbi | 认知功能障碍疾病的生物标记物及使用该生物标记物的认知功能障碍疾病的检测方法 |
| EP3022322A4 (en) | 2013-07-17 | 2017-05-17 | The Johns Hopkins University | A multi-protein biomarker assay for brain injury detection and outcome |
| MA40636A (fr) | 2014-09-11 | 2015-09-11 | Colleen Kelly | Procédés pour détecter le cancer de la prostate |
| FI20155280L (fi) * | 2015-04-15 | 2016-10-16 | Medicortex Finland Oy | Aivovamman prognostisia ja diagnostisia glykaanipohjaisia biomarkkereita |
| CN105506083B (zh) * | 2015-12-24 | 2018-10-19 | 孙梅芬 | Capg在制备诊断帕金森症产品中的用途 |
| JP6947451B2 (ja) * | 2016-06-29 | 2021-10-13 | 学校法人自治医科大学 | バイオマーカー、診断用組成物、及び診断用キット |
| WO2018002362A1 (en) * | 2016-06-30 | 2018-01-04 | Randox Laboratories Ltd | Measurement of fabp for diagnosis |
| EP3506927A4 (en) | 2016-08-03 | 2020-05-06 | University of South Florida | REELIN COMPOSITIONS FOR TREATING NEUROLOGICAL DISEASES |
| WO2018030252A1 (ja) * | 2016-08-09 | 2018-02-15 | 大塚製薬株式会社 | 尿バイオマーカーを用いたアルツハイマー病の診断補助方法 |
| US10914748B2 (en) | 2016-09-08 | 2021-02-09 | UNIVERSITé LAVAL | Erythrocyte-derived extracellular vesicles as a biomarker for clinically assessing Parkinson's disease |
| KR102806918B1 (ko) * | 2016-09-16 | 2025-05-14 | 다케다 파머수티컬 컴패니 리미티드 | 접촉 활성화 시스템과 연관된 질환을 위한 단백질 바이오마커 |
| WO2018081649A1 (en) | 2016-10-28 | 2018-05-03 | Banyan Biomarkers, Inc. | Antibodies to ubiquitin c-terminal hydrolase l1 (uch-l1) and glial fibrillary acidic protein (gfap) and related methods |
| KR102466369B1 (ko) * | 2020-08-04 | 2022-11-14 | 서울대학교병원 | 자가면역 뇌염의 진단 방법 |
| JP7569552B2 (ja) * | 2021-01-29 | 2024-10-18 | 国立大学法人東北大学 | 認知症診断用のバイオマーカー |
| CN114966045A (zh) * | 2022-05-07 | 2022-08-30 | 浙江大学 | 检测抗肌球蛋白轻链1-IgG自身抗体的试剂在制备检测血管内皮损伤的试剂盒中的应用 |
| CN115166260B (zh) * | 2022-07-11 | 2023-06-13 | 东南大学 | 血浆脑细胞来源外泌体中维生素d结合蛋白在诊断抑郁症中的应用 |
| CN118624905B (zh) * | 2023-07-31 | 2025-04-01 | 中国人民解放军总医院第一医学中心 | 一种糖尿病性肾病诊断的生物标志物和试剂盒及诊断设备 |
| CN120820725B (zh) * | 2025-09-17 | 2026-01-27 | 上海交通大学医学院附属仁济医院 | 胶质纤维酸性蛋白和乳酸脱氢酶a亚型在制备诊断颅脑创伤的试剂盒中的用途 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4017A (en) * | 1845-05-01 | Reid r | ||
| US3015A (en) * | 1843-03-21 | Improvement in gilding, silvering | ||
| GB225245A (en) * | 1923-11-23 | 1924-12-24 | British Thomson Houston Co Ltd | Improvements in and relating to elastic fluid turbines |
| GB322063A (en) * | 1928-10-29 | 1929-11-28 | Godwin Stanley Oliver | Improvements relating to automatic vehicle-controlled track-signalling devices |
| JPH09110713A (ja) * | 1995-10-13 | 1997-04-28 | Nippon Green Ueebu Kk | グルタチオン−ｓ−トランスフェラーゼ活性化剤並びにそれを含む医薬品及び飲食物 |
| EP0914452A2 (en) * | 1996-06-17 | 1999-05-12 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Novel ptp20, pcp-2, bdp1, clk and sirp proteins and related products and methods |
| US6541615B1 (en) * | 1996-11-15 | 2003-04-01 | Max-Planck-Gellschaft Zur Foderung Der Wissenschaften E.V. | SIRP proteins and uses thereof |
| CA2263063C (en) * | 1999-02-26 | 2004-08-10 | Skye Pharmatech Incorporated | Method for diagnosing and distinguishing stroke and diagnostic devices for use therein |
| AU1701001A (en) * | 1999-11-30 | 2001-06-12 | Eberhard-Karls-Universitat Tubingen Universitatsklinikum | Antibodies against signal regulator proteins |
| GB9929140D0 (en) | 1999-12-10 | 2000-02-02 | Univ Geneve | Diagnostic assay for stroke |
| ATE368858T1 (de) | 2000-03-10 | 2007-08-15 | Univ Geneve | Verfahren zur diagnose von transmissiblen spongiformen encephalopathien |
| GB2360089A (en) * | 2000-03-10 | 2001-09-12 | Univ Geneve | Diagnostic assay for transmisible spongiform encephalopathies |
| US7608406B2 (en) * | 2001-08-20 | 2009-10-27 | Biosite, Inc. | Diagnostic markers of stroke and cerebral injury and methods of use thereof |
| AU2003274368B2 (en) * | 2002-10-30 | 2008-11-20 | Proteome Sciences Plc. | Diagnostic method for transmissible spongiform encephalopathies (prion diseases) |
| DK2357477T3 (en) * | 2003-09-20 | 2018-02-05 | Electrophoretics Ltd | Diagnostic approach for brain injury-related disorders based on detection of NDKA |
| JP2007518062A (ja) * | 2003-09-29 | 2007-07-05 | バイオサイト インコーポレイテッド | 敗血症を診断する方法および診断するための組成物 |
| GB0419124D0 (en) | 2004-08-27 | 2004-09-29 | Proteome Sciences Plc | Methods and compositions relating to Alzheimer's disease |
| GB0421639D0 (en) | 2004-09-29 | 2004-10-27 | Proteome Sciences Plc | Methods and compositions relating to alzheimer's disease |
| EP1825274B1 (en) | 2004-12-07 | 2009-11-18 | Electrophoretics Limited | Monitoring Huntington's disease |
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| AU2006268034A1 (en) | 2007-01-18 |
| CA2613991A1 (en) | 2007-01-18 |
| JP2013092538A (ja) | 2013-05-16 |
| JP2009501333A (ja) | 2009-01-15 |
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