ES2262249T3 - Metodo fluorometrico para monitorizar y detectar la ampliacion de acidos nucleicos. - Google Patents
Metodo fluorometrico para monitorizar y detectar la ampliacion de acidos nucleicos.Info
- Publication number
- ES2262249T3 ES2262249T3 ES98955810T ES98955810T ES2262249T3 ES 2262249 T3 ES2262249 T3 ES 2262249T3 ES 98955810 T ES98955810 T ES 98955810T ES 98955810 T ES98955810 T ES 98955810T ES 2262249 T3 ES2262249 T3 ES 2262249T3
- Authority
- ES
- Spain
- Prior art keywords
- molecule
- probe
- donor
- acceptor
- amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title claims abstract description 58
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 13
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 13
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 13
- 238000012544 monitoring process Methods 0.000 title 1
- 239000000523 sample Substances 0.000 claims abstract description 95
- 230000003321 amplification Effects 0.000 claims abstract description 72
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 72
- 108091008146 restriction endonucleases Proteins 0.000 claims abstract description 49
- 108091033319 polynucleotide Proteins 0.000 claims abstract description 42
- 102000040430 polynucleotide Human genes 0.000 claims abstract description 42
- 239000002157 polynucleotide Substances 0.000 claims abstract description 41
- 230000000295 complement effect Effects 0.000 claims abstract description 15
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims abstract description 14
- 239000002751 oligonucleotide probe Substances 0.000 claims abstract description 14
- 238000000926 separation method Methods 0.000 claims abstract description 7
- 102000004190 Enzymes Human genes 0.000 claims description 50
- 108090000790 Enzymes Proteins 0.000 claims description 50
- 238000006243 chemical reaction Methods 0.000 claims description 50
- 108091093088 Amplicon Proteins 0.000 claims description 26
- 125000003729 nucleotide group Chemical group 0.000 claims description 26
- 108020004414 DNA Proteins 0.000 claims description 19
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims description 16
- 230000000694 effects Effects 0.000 claims description 14
- 239000002773 nucleotide Substances 0.000 claims description 14
- 239000000975 dye Substances 0.000 claims description 10
- 102000053602 DNA Human genes 0.000 claims description 8
- 108091034117 Oligonucleotide Proteins 0.000 claims description 4
- 238000005520 cutting process Methods 0.000 claims description 4
- 238000005304 joining Methods 0.000 claims description 3
- 238000003752 polymerase chain reaction Methods 0.000 claims description 3
- 239000011541 reaction mixture Substances 0.000 claims description 3
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 claims description 3
- 230000004888 barrier function Effects 0.000 claims description 2
- 238000005259 measurement Methods 0.000 claims 1
- 230000000717 retained effect Effects 0.000 claims 1
- 239000001022 rhodamine dye Substances 0.000 claims 1
- 239000000370 acceptor Substances 0.000 description 42
- 238000001514 detection method Methods 0.000 description 12
- 239000012634 fragment Substances 0.000 description 9
- 238000009396 hybridization Methods 0.000 description 7
- 108010042407 Endonucleases Proteins 0.000 description 6
- 102000004533 Endonucleases Human genes 0.000 description 6
- 230000000692 anti-sense effect Effects 0.000 description 5
- 238000003556 assay Methods 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 230000029087 digestion Effects 0.000 description 4
- 239000013642 negative control Substances 0.000 description 4
- 230000007704 transition Effects 0.000 description 4
- 239000002253 acid Substances 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 101710163270 Nuclease Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 108010006785 Taq Polymerase Proteins 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 238000000246 agarose gel electrophoresis Methods 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 239000000155 melt Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000026731 phosphorylation Effects 0.000 description 2
- 238000006366 phosphorylation reaction Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 102100034330 Chromaffin granule amine transporter Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 101000641221 Homo sapiens Chromaffin granule amine transporter Proteins 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241001467519 Pyrococcus sp. Species 0.000 description 1
- 241001122767 Theaceae Species 0.000 description 1
- 241000589500 Thermus aquaticus Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 108010027697 Type I Site-Specific Deoxyribonucleases Proteins 0.000 description 1
- 108010064978 Type II Site-Specific Deoxyribonucleases Proteins 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000003570 air Substances 0.000 description 1
- 239000012080 ambient air Substances 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000000386 donor Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000000138 intercalating agent Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 108010068698 spleen exonuclease Proteins 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Amplifiers (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9725237 | 1997-11-29 | ||
| GBGB9725237.3A GB9725237D0 (en) | 1997-11-29 | 1997-11-29 | Amplification system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2262249T3 true ES2262249T3 (es) | 2006-11-16 |
Family
ID=10822822
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES98955810T Expired - Lifetime ES2262249T3 (es) | 1997-11-29 | 1998-11-27 | Metodo fluorometrico para monitorizar y detectar la ampliacion de acidos nucleicos. |
| ES06005863T Expired - Lifetime ES2301106T3 (es) | 1997-11-29 | 1998-11-27 | Metodo fluorimetrico para el seguimiento y la deteccion de la amplificacion de acidos nucleicos. |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES06005863T Expired - Lifetime ES2301106T3 (es) | 1997-11-29 | 1998-11-27 | Metodo fluorimetrico para el seguimiento y la deteccion de la amplificacion de acidos nucleicos. |
Country Status (11)
| Country | Link |
|---|---|
| EP (2) | EP1044283B2 (enExample) |
| JP (1) | JP4535615B2 (enExample) |
| KR (1) | KR20010032598A (enExample) |
| AT (2) | ATE325893T1 (enExample) |
| AU (1) | AU744092B2 (enExample) |
| CA (1) | CA2311931C (enExample) |
| DE (2) | DE69839235T3 (enExample) |
| ES (2) | ES2262249T3 (enExample) |
| GB (3) | GB9725237D0 (enExample) |
| NZ (1) | NZ504691A (enExample) |
| WO (1) | WO1999028501A1 (enExample) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9827908D0 (en) * | 1998-12-19 | 1999-02-10 | Univ Manchester | Nucleic acid sequencing method |
| AUPP844899A0 (en) * | 1999-02-01 | 1999-02-25 | University Of Western Australia, The | A method for detecting methylated nucleic acids |
| GB9918237D0 (en) * | 1999-08-04 | 1999-10-06 | Secr Defence | Detection system |
| GB0110501D0 (en) | 2001-04-30 | 2001-06-20 | Secr Defence Brit | Amplification process |
| GB0111275D0 (en) | 2001-05-09 | 2001-06-27 | Secr Defence | Analytical method and kit |
| GB0112868D0 (en) | 2001-05-25 | 2001-07-18 | Secr Defence | Detection system |
| US9261460B2 (en) | 2002-03-12 | 2016-02-16 | Enzo Life Sciences, Inc. | Real-time nucleic acid detection processes and compositions |
| US9353405B2 (en) | 2002-03-12 | 2016-05-31 | Enzo Life Sciences, Inc. | Optimized real time nucleic acid detection processes |
| GB0304832D0 (en) | 2003-03-04 | 2003-04-09 | Secr Defence | Assay method |
| DE10353419B3 (de) * | 2003-11-09 | 2005-06-30 | Epigenomics Ag | Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA-Sequenzen mittels hemimethylierungssensitiver Restriktionsenzyme |
| GB0503172D0 (en) | 2005-02-16 | 2005-03-23 | Enigma Diagnostics Ltd | Detection method |
| GB0517005D0 (en) | 2005-08-19 | 2005-09-28 | Enigma Diagnostics Ltd | Analytical method and kit |
| EP1969140A2 (en) * | 2005-11-23 | 2008-09-17 | DiaSorin S.p.A. | Reagents and method for simultaneous nucleic acid amplification and detection |
| EP1905842A1 (en) * | 2006-09-26 | 2008-04-02 | DiaSorin S.p.A. | Method for detection of mutant alleles combining Real Time PCR and REMS-PCR |
| US8911948B2 (en) | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| US9434988B2 (en) | 2008-04-30 | 2016-09-06 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| EP3150727B1 (en) | 2008-04-30 | 2019-07-10 | Integrated DNA Technologies Inc. | Rnase-h-based assays utilizing modified rna monomers |
| JP6440997B2 (ja) | 2014-08-22 | 2018-12-19 | 国立大学法人大阪大学 | Ｐｃｒ法およびｐｃｒキット |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2176348C (en) * | 1993-11-12 | 2004-11-02 | Sanjay Tyagi | Hybridization probes for nucleic acid detection, universal stems, methods and kits |
| US5648211A (en) † | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| DE4419566A1 (de) * | 1994-06-03 | 1995-12-07 | Birsner & Grob Biotech Gmbh | Kompartimentiertes Reaktionsgefäß |
| EP0803058B1 (en) † | 1994-12-30 | 2008-11-05 | Georgetown University | Fluorometric assay for detecting nucleic acid cleavage |
| US5955268A (en) * | 1996-04-26 | 1999-09-21 | Abbott Laboratories | Method and reagent for detecting multiple nucleic acid sequences in a test sample |
| US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US5691145A (en) * | 1996-08-27 | 1997-11-25 | Becton, Dickinson And Company | Detection of nucleic acids using G-quartets |
| US5846726A (en) * | 1997-05-13 | 1998-12-08 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| US5928869A (en) * | 1997-05-30 | 1999-07-27 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| JPH1156398A (ja) * | 1997-08-11 | 1999-03-02 | Hamamatsu Photonics Kk | 二本鎖核酸分解酵素活性の測定方法 |
| US5935791A (en) * | 1997-09-23 | 1999-08-10 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
-
1997
- 1997-11-29 GB GBGB9725237.3A patent/GB9725237D0/en not_active Ceased
-
1998
- 1998-11-27 WO PCT/GB1998/003563 patent/WO1999028501A1/en not_active Ceased
- 1998-11-27 AT AT98955810T patent/ATE325893T1/de not_active IP Right Cessation
- 1998-11-27 AU AU12533/99A patent/AU744092B2/en not_active Ceased
- 1998-11-27 JP JP2000523376A patent/JP4535615B2/ja not_active Expired - Fee Related
- 1998-11-27 DE DE69839235.3T patent/DE69839235T3/de not_active Expired - Lifetime
- 1998-11-27 AT AT06005863T patent/ATE388245T1/de not_active IP Right Cessation
- 1998-11-27 NZ NZ504691A patent/NZ504691A/en not_active IP Right Cessation
- 1998-11-27 DE DE69834506T patent/DE69834506T2/de not_active Expired - Lifetime
- 1998-11-27 CA CA2311931A patent/CA2311931C/en not_active Expired - Lifetime
- 1998-11-27 KR KR1020007005866A patent/KR20010032598A/ko not_active Ceased
- 1998-11-27 GB GB9825925A patent/GB2333596A/en not_active Withdrawn
- 1998-11-27 EP EP98955810.1A patent/EP1044283B2/en not_active Expired - Lifetime
- 1998-11-27 EP EP06005863.3A patent/EP1686191B3/en not_active Expired - Lifetime
- 1998-11-27 ES ES98955810T patent/ES2262249T3/es not_active Expired - Lifetime
- 1998-11-27 ES ES06005863T patent/ES2301106T3/es not_active Expired - Lifetime
- 1998-11-27 GB GB0011451A patent/GB2346695B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| WO1999028501A1 (en) | 1999-06-10 |
| EP1044283B2 (en) | 2015-09-23 |
| DE69839235D1 (de) | 2008-04-17 |
| DE69834506T2 (de) | 2007-04-19 |
| DE69839235T3 (de) | 2017-02-23 |
| EP1686191B3 (en) | 2016-07-20 |
| GB2346695A (en) | 2000-08-16 |
| AU1253399A (en) | 1999-06-16 |
| EP1686191A3 (en) | 2006-09-13 |
| AU744092B2 (en) | 2002-02-14 |
| GB0011451D0 (en) | 2000-06-28 |
| KR20010032598A (ko) | 2001-04-25 |
| GB2346695B (en) | 2002-10-30 |
| ATE325893T1 (de) | 2006-06-15 |
| EP1044283A1 (en) | 2000-10-18 |
| NZ504691A (en) | 2002-02-01 |
| EP1044283B1 (en) | 2006-05-10 |
| EP1686191A2 (en) | 2006-08-02 |
| GB9825925D0 (en) | 1999-01-20 |
| GB9725237D0 (en) | 1998-01-28 |
| ES2301106T3 (es) | 2008-06-16 |
| JP4535615B2 (ja) | 2010-09-01 |
| ATE388245T1 (de) | 2008-03-15 |
| GB2333596A (en) | 1999-07-28 |
| DE69834506D1 (de) | 2006-06-14 |
| CA2311931A1 (en) | 1999-06-10 |
| CA2311931C (en) | 2011-01-04 |
| JP2002507719A (ja) | 2002-03-12 |
| EP1686191B1 (en) | 2008-03-05 |
| DE69839235T2 (de) | 2009-02-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2262249T3 (es) | Metodo fluorometrico para monitorizar y detectar la ampliacion de acidos nucleicos. | |
| ES2232972T3 (es) | Sistema fluorimetrico de deteccion de un acido nucleico. | |
| ES2254505T3 (es) | Metodo para la amplificacion y caracterizacion opcional de acidos nucleicos. | |
| RU2608501C2 (ru) | Детекция нуклеиновокислотной последовательности-мишени в анализе с гибридизацией сигнального олигонуклеотида, зависящей от расщепления и удлинения зондирующего и метящего олигонуклеотида (варианты) | |
| CN103534358B (zh) | 基于pto切割以及延伸-依赖性切割的靶核酸序列的检测 | |
| ES2414286T3 (es) | Detección de ácidos nucleicos | |
| CN102959092B (zh) | 借助于探测和标记的寡核苷酸切割以及延长试验的靶核酸序列检测 | |
| ES2653642T3 (es) | Detección de una secuencia de ácido nucleico diana mediante ensayo sin hibridación dependiente de escisión y extensión de PTO | |
| JP5770738B2 (ja) | Ｔｈｄプライマーターゲット検出 | |
| JP6381659B2 (ja) | メチル化検出方法 | |
| ES2669244T3 (es) | Detección de variación de nucleótidos en una secuencia de ácido nucleico diana mediante escisión CTO y ensayo de extensión | |
| CA2658071A1 (en) | Specialized oligonucleotides and their use in nucleic acid amplification and detection | |
| JP6096885B2 (ja) | ＰＴＯ切断及び伸長依存的なシグナリングオリゴヌクレオチド切断を用いたターゲット核酸配列の検出｛ＤｅｔｅｃｔｉｏｎｏｆＴａｒｇｅｔＮｕｃｌｅｉｃＡｃｉｄＳｅｑｕｅｎｃｅｂｙＰＴＯＣｌｅａｖａｇｅａｎｄＥｘｔｅｎｓｉｏｎ−ＤｅｐｅｎｄｅｎｔＳｉｇｎａｌｉｎｇＯｌｉｇｏｎｕｃｌｅｏｔｉｄｅＣｌｅａｖａｇｅ｝ | |
| ES2256067T3 (es) | Oligonucleotidos y combinaciones de los mismos utiles en la deteccion de la presencia o ausencia de secuencias de acidos nucleicos diana en una muestra. | |
| ES2281880T3 (es) | Procedimiento de deteccion de acidos nucleicos que usa multiples pares de fluoroforos donadores y moleculas inhibidoras de la fluorescencia en la misma sonda. | |
| CN105358710B (zh) | 靶核酸序列上的核苷酸变异的检测 | |
| JP2002507719A5 (enExample) | ||
| BRPI0715427A2 (pt) | mÉtodo para amplificaÇço de sequÊncia ou sinal de Ácidos nucleicos alvo, oligonucleotideo mofificado reversÍvel, mistura e conjunto de reaÇço de amplificaÇço de Ácido nucleico | |
| KR20200038338A (ko) | Pto 절단 및 연장-의존적 연장 분석에 의한 타겟 핵산 서열의 검출 | |
| WO2006136621A1 (es) | Método de detección de ácidos nucleicos mediante generación directa de una señal medible | |
| ES2305233T3 (es) | Procedimiento y equipo analitico. | |
| ES2245315T3 (es) | Metodo de amplificacion para la deteccion de acidos nucleicos diana que implica transferencia de energia fluorescente. | |
| ES2997119T3 (en) | Combined product for testing dna | |
| CN105705657A (zh) | 基于利用杂交-捕捉和模板化寡核苷酸的探测和标记寡核苷酸切割及延伸分析的在固相中的靶核酸序列检测 |