CA2311931C - Fluorometric method for monitoring and detecting nucleic acid amplification - Google Patents
Fluorometric method for monitoring and detecting nucleic acid amplification Download PDFInfo
- Publication number
- CA2311931C CA2311931C CA2311931A CA2311931A CA2311931C CA 2311931 C CA2311931 C CA 2311931C CA 2311931 A CA2311931 A CA 2311931A CA 2311931 A CA2311931 A CA 2311931A CA 2311931 C CA2311931 C CA 2311931C
- Authority
- CA
- Canada
- Prior art keywords
- probe
- donor
- region
- molecule
- restriction enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Amplifiers (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9725237.3 | 1997-11-29 | ||
| GBGB9725237.3A GB9725237D0 (en) | 1997-11-29 | 1997-11-29 | Amplification system |
| PCT/GB1998/003563 WO1999028501A1 (en) | 1997-11-29 | 1998-11-27 | Fluorometric method for monitoring and detecting nucleic acid amplification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2311931A1 CA2311931A1 (en) | 1999-06-10 |
| CA2311931C true CA2311931C (en) | 2011-01-04 |
Family
ID=10822822
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2311931A Expired - Lifetime CA2311931C (en) | 1997-11-29 | 1998-11-27 | Fluorometric method for monitoring and detecting nucleic acid amplification |
Country Status (11)
| Country | Link |
|---|---|
| EP (2) | EP1686191B3 (enExample) |
| JP (1) | JP4535615B2 (enExample) |
| KR (1) | KR20010032598A (enExample) |
| AT (2) | ATE388245T1 (enExample) |
| AU (1) | AU744092B2 (enExample) |
| CA (1) | CA2311931C (enExample) |
| DE (2) | DE69834506T2 (enExample) |
| ES (2) | ES2301106T3 (enExample) |
| GB (3) | GB9725237D0 (enExample) |
| NZ (1) | NZ504691A (enExample) |
| WO (1) | WO1999028501A1 (enExample) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9827908D0 (en) * | 1998-12-19 | 1999-02-10 | Univ Manchester | Nucleic acid sequencing method |
| AUPP844899A0 (en) * | 1999-02-01 | 1999-02-25 | University Of Western Australia, The | A method for detecting methylated nucleic acids |
| GB9918237D0 (en) * | 1999-08-04 | 1999-10-06 | Secr Defence | Detection system |
| GB0110501D0 (en) | 2001-04-30 | 2001-06-20 | Secr Defence Brit | Amplification process |
| GB0111275D0 (en) | 2001-05-09 | 2001-06-27 | Secr Defence | Analytical method and kit |
| GB0112868D0 (en) | 2001-05-25 | 2001-07-18 | Secr Defence | Detection system |
| US9261460B2 (en) | 2002-03-12 | 2016-02-16 | Enzo Life Sciences, Inc. | Real-time nucleic acid detection processes and compositions |
| US9353405B2 (en) | 2002-03-12 | 2016-05-31 | Enzo Life Sciences, Inc. | Optimized real time nucleic acid detection processes |
| GB0304832D0 (en) | 2003-03-04 | 2003-04-09 | Secr Defence | Assay method |
| DE10353419B3 (de) * | 2003-11-09 | 2005-06-30 | Epigenomics Ag | Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA-Sequenzen mittels hemimethylierungssensitiver Restriktionsenzyme |
| GB0503172D0 (en) | 2005-02-16 | 2005-03-23 | Enigma Diagnostics Ltd | Detection method |
| GB0517005D0 (en) | 2005-08-19 | 2005-09-28 | Enigma Diagnostics Ltd | Analytical method and kit |
| WO2007060707A2 (en) * | 2005-11-23 | 2007-05-31 | Diasorin Spa | Reagents and method for simultaneous nucleic acid amplification and detection |
| EP1905842A1 (en) * | 2006-09-26 | 2008-04-02 | DiaSorin S.p.A. | Method for detection of mutant alleles combining Real Time PCR and REMS-PCR |
| WO2009135093A2 (en) | 2008-04-30 | 2009-11-05 | Integrated Dna Technologies, Inc. | Rnase-h-based assays utilizing modified rna monomers |
| US8911948B2 (en) | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| AU2012236896A1 (en) | 2011-03-25 | 2013-05-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| JP6440997B2 (ja) * | 2014-08-22 | 2018-12-19 | 国立大学法人大阪大学 | Ｐｃｒ法およびｐｃｒキット |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT728218E (pt) * | 1993-11-12 | 2008-04-08 | Phri Properties Inc | Sondas de hibridação para detecção de ácidos nucleicos, troncos universais, métodos e estojos |
| US5648211A (en) † | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| DE4419566A1 (de) * | 1994-06-03 | 1995-12-07 | Birsner & Grob Biotech Gmbh | Kompartimentiertes Reaktionsgefäß |
| CA2209080C (en) † | 1994-12-30 | 2008-07-29 | Georgetown University | Fluorometric assay for detecting nucleic acid cleavage |
| US5955268A (en) * | 1996-04-26 | 1999-09-21 | Abbott Laboratories | Method and reagent for detecting multiple nucleic acid sequences in a test sample |
| US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US5691145A (en) * | 1996-08-27 | 1997-11-25 | Becton, Dickinson And Company | Detection of nucleic acids using G-quartets |
| US5846726A (en) † | 1997-05-13 | 1998-12-08 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| US5928869A (en) * | 1997-05-30 | 1999-07-27 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| JPH1156398A (ja) * | 1997-08-11 | 1999-03-02 | Hamamatsu Photonics Kk | 二本鎖核酸分解酵素活性の測定方法 |
| US5935791A (en) * | 1997-09-23 | 1999-08-10 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
-
1997
- 1997-11-29 GB GBGB9725237.3A patent/GB9725237D0/en not_active Ceased
-
1998
- 1998-11-27 ES ES06005863T patent/ES2301106T3/es not_active Expired - Lifetime
- 1998-11-27 DE DE69834506T patent/DE69834506T2/de not_active Expired - Lifetime
- 1998-11-27 WO PCT/GB1998/003563 patent/WO1999028501A1/en not_active Ceased
- 1998-11-27 NZ NZ504691A patent/NZ504691A/en not_active IP Right Cessation
- 1998-11-27 DE DE69839235.3T patent/DE69839235T3/de not_active Expired - Lifetime
- 1998-11-27 AT AT06005863T patent/ATE388245T1/de not_active IP Right Cessation
- 1998-11-27 AT AT98955810T patent/ATE325893T1/de not_active IP Right Cessation
- 1998-11-27 GB GB9825925A patent/GB2333596A/en not_active Withdrawn
- 1998-11-27 GB GB0011451A patent/GB2346695B/en not_active Expired - Fee Related
- 1998-11-27 EP EP06005863.3A patent/EP1686191B3/en not_active Expired - Lifetime
- 1998-11-27 AU AU12533/99A patent/AU744092B2/en not_active Ceased
- 1998-11-27 EP EP98955810.1A patent/EP1044283B2/en not_active Expired - Lifetime
- 1998-11-27 KR KR1020007005866A patent/KR20010032598A/ko not_active Ceased
- 1998-11-27 JP JP2000523376A patent/JP4535615B2/ja not_active Expired - Fee Related
- 1998-11-27 ES ES98955810T patent/ES2262249T3/es not_active Expired - Lifetime
- 1998-11-27 CA CA2311931A patent/CA2311931C/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| ES2262249T3 (es) | 2006-11-16 |
| EP1044283B1 (en) | 2006-05-10 |
| EP1686191A2 (en) | 2006-08-02 |
| ATE388245T1 (de) | 2008-03-15 |
| JP2002507719A (ja) | 2002-03-12 |
| ATE325893T1 (de) | 2006-06-15 |
| GB2346695B (en) | 2002-10-30 |
| KR20010032598A (ko) | 2001-04-25 |
| EP1686191B1 (en) | 2008-03-05 |
| GB9725237D0 (en) | 1998-01-28 |
| DE69839235D1 (de) | 2008-04-17 |
| GB2333596A (en) | 1999-07-28 |
| EP1686191A3 (en) | 2006-09-13 |
| GB9825925D0 (en) | 1999-01-20 |
| DE69839235T2 (de) | 2009-02-26 |
| JP4535615B2 (ja) | 2010-09-01 |
| WO1999028501A1 (en) | 1999-06-10 |
| DE69834506D1 (de) | 2006-06-14 |
| GB0011451D0 (en) | 2000-06-28 |
| AU744092B2 (en) | 2002-02-14 |
| DE69834506T2 (de) | 2007-04-19 |
| GB2346695A (en) | 2000-08-16 |
| NZ504691A (en) | 2002-02-01 |
| EP1044283B2 (en) | 2015-09-23 |
| EP1044283A1 (en) | 2000-10-18 |
| DE69839235T3 (de) | 2017-02-23 |
| CA2311931A1 (en) | 1999-06-10 |
| AU1253399A (en) | 1999-06-16 |
| EP1686191B3 (en) | 2016-07-20 |
| ES2301106T3 (es) | 2008-06-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2311931C (en) | Fluorometric method for monitoring and detecting nucleic acid amplification | |
| JP6005180B2 (ja) | ＰＴＯ切断及び伸長依存的なシグナリングオリゴヌクレオチドハイブリダイゼーションを用いたターゲット核酸配列の検出（ＤｅｔｅｃｔｉｏｎｏｆＴａｒｇｅｔＮｕｃｌｅｉｃＡｃｉｄＳｅｑｕｅｎｃｅｂｙＰＴＯＣｌｅａｖａｇｅａｎｄＥｘｔｅｎｓｉｏｎ−ＤｅｐｅｎｄｅｎｔＳｉｇｎａｌｉｎｇＯｌｉｇｏｎｕｃｌｅｏｔｉｄｅＨｙｂｒｉｄｉｚａｔｉｏｎＡｓｓａｙ） | |
| EP1066414B1 (en) | Rolling circle replication of padlock probes | |
| KR101569479B1 (ko) | Ｐｔｏ 절단 및 연장-의존적 절단에 의한 타겟 핵산서열의 검출 | |
| USRE44265E1 (en) | Nucleic acid amplification method | |
| EP3543352B1 (en) | Detection of target nucleic acid sequences by pto cleavage and extension assay | |
| KR101794981B1 (ko) | Ｐｔｏ 절단 및 연장 어세이에 의한 타겟 핵산서열에서의 뉴클레오타이드 변이 검출 | |
| EP2373808B1 (en) | Enhanced taqman probe based amplification | |
| KR101756975B1 (ko) | Ｐｔｏ 절단 및 연장-의존적 시그널링 올리고뉴클레오타이드 절단을 이용한 타겟 핵산서열의 검출 | |
| CN105358710B (zh) | 靶核酸序列上的核苷酸变异的检测 | |
| KR20200038338A (ko) | Pto 절단 및 연장-의존적 연장 분석에 의한 타겟 핵산 서열의 검출 | |
| KR20120105811A (ko) | Ｐｔｏ 절단 및 연장-의존적 시그널 발생에 의한 타겟 핵산서열의 검출 | |
| KR20170142128A (ko) | Cto 세트를 사용한 pto 절단 및 연장 어세이에 의한 타겟 핵산서열의 검출 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20181127 |