JP2002507719A5 - - Google Patents
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- Publication number
- JP2002507719A5 JP2002507719A5 JP2000523376A JP2000523376A JP2002507719A5 JP 2002507719 A5 JP2002507719 A5 JP 2002507719A5 JP 2000523376 A JP2000523376 A JP 2000523376A JP 2000523376 A JP2000523376 A JP 2000523376A JP 2002507719 A5 JP2002507719 A5 JP 2002507719A5
- Authority
- JP
- Japan
- Prior art keywords
- probe
- molecule
- donor
- nucleotide
- restriction enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 description 32
- 239000000523 sample Substances 0.000 description 20
- 125000003729 nucleotide group Chemical group 0.000 description 14
- 108091008146 restriction endonucleases Proteins 0.000 description 14
- 230000003321 amplification Effects 0.000 description 13
- 238000003199 nucleic acid amplification method Methods 0.000 description 13
- 239000002157 polynucleotide Substances 0.000 description 10
- 239000002773 nucleotide Substances 0.000 description 9
- 108091033319 polynucleotide Proteins 0.000 description 9
- 102000040430 polynucleotide Human genes 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 6
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 5
- 239000002751 oligonucleotide probe Substances 0.000 description 5
- 102000039446 nucleic acids Human genes 0.000 description 4
- 108020004707 nucleic acids Proteins 0.000 description 4
- 150000007523 nucleic acids Chemical class 0.000 description 4
- 230000000295 complement effect Effects 0.000 description 3
- 108091093088 Amplicon Proteins 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 239000001022 rhodamine dye Substances 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 101100379079 Emericella variicolor andA gene Proteins 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9725237.3A GB9725237D0 (en) | 1997-11-29 | 1997-11-29 | Amplification system |
| GB9725237.3 | 1997-11-29 | ||
| PCT/GB1998/003563 WO1999028501A1 (en) | 1997-11-29 | 1998-11-27 | Fluorometric method for monitoring and detecting nucleic acid amplification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2002507719A JP2002507719A (ja) | 2002-03-12 |
| JP2002507719A5 true JP2002507719A5 (enExample) | 2006-01-26 |
| JP4535615B2 JP4535615B2 (ja) | 2010-09-01 |
Family
ID=10822822
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000523376A Expired - Fee Related JP4535615B2 (ja) | 1997-11-29 | 1998-11-27 | 核酸増幅のモニター及び検出のための蛍光定量法 |
Country Status (11)
| Country | Link |
|---|---|
| EP (2) | EP1044283B2 (enExample) |
| JP (1) | JP4535615B2 (enExample) |
| KR (1) | KR20010032598A (enExample) |
| AT (2) | ATE388245T1 (enExample) |
| AU (1) | AU744092B2 (enExample) |
| CA (1) | CA2311931C (enExample) |
| DE (2) | DE69839235T3 (enExample) |
| ES (2) | ES2262249T3 (enExample) |
| GB (3) | GB9725237D0 (enExample) |
| NZ (1) | NZ504691A (enExample) |
| WO (1) | WO1999028501A1 (enExample) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9827908D0 (en) * | 1998-12-19 | 1999-02-10 | Univ Manchester | Nucleic acid sequencing method |
| AUPP844899A0 (en) * | 1999-02-01 | 1999-02-25 | University Of Western Australia, The | A method for detecting methylated nucleic acids |
| GB9918237D0 (en) | 1999-08-04 | 1999-10-06 | Secr Defence | Detection system |
| GB0110501D0 (en) | 2001-04-30 | 2001-06-20 | Secr Defence Brit | Amplification process |
| GB0111275D0 (en) | 2001-05-09 | 2001-06-27 | Secr Defence | Analytical method and kit |
| GB0112868D0 (en) | 2001-05-25 | 2001-07-18 | Secr Defence | Detection system |
| US9261460B2 (en) | 2002-03-12 | 2016-02-16 | Enzo Life Sciences, Inc. | Real-time nucleic acid detection processes and compositions |
| US9353405B2 (en) | 2002-03-12 | 2016-05-31 | Enzo Life Sciences, Inc. | Optimized real time nucleic acid detection processes |
| GB0304832D0 (en) | 2003-03-04 | 2003-04-09 | Secr Defence | Assay method |
| DE10353419B3 (de) * | 2003-11-09 | 2005-06-30 | Epigenomics Ag | Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA-Sequenzen mittels hemimethylierungssensitiver Restriktionsenzyme |
| GB0503172D0 (en) | 2005-02-16 | 2005-03-23 | Enigma Diagnostics Ltd | Detection method |
| GB0517005D0 (en) | 2005-08-19 | 2005-09-28 | Enigma Diagnostics Ltd | Analytical method and kit |
| WO2007060707A2 (en) * | 2005-11-23 | 2007-05-31 | Diasorin Spa | Reagents and method for simultaneous nucleic acid amplification and detection |
| EP1905842A1 (en) * | 2006-09-26 | 2008-04-02 | DiaSorin S.p.A. | Method for detection of mutant alleles combining Real Time PCR and REMS-PCR |
| US8911948B2 (en) | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| EP2279263B1 (en) | 2008-04-30 | 2013-09-04 | Integrated Dna Technologies, Inc. | Rnase-h-based assays utilizing modified rna monomers |
| WO2012135053A2 (en) | 2011-03-25 | 2012-10-04 | Integrated Dna Technologies, Inc. | Rnase h-based assays utilizing modified rna monomers |
| JP6440997B2 (ja) * | 2014-08-22 | 2018-12-19 | 国立大学法人大阪大学 | Ｐｃｒ法およびｐｃｒキット |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT728218E (pt) * | 1993-11-12 | 2008-04-08 | Phri Properties Inc | Sondas de hibridação para detecção de ácidos nucleicos, troncos universais, métodos e estojos |
| US5648211A (en) † | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| DE4419566A1 (de) * | 1994-06-03 | 1995-12-07 | Birsner & Grob Biotech Gmbh | Kompartimentiertes Reaktionsgefäß |
| CA2209080C (en) † | 1994-12-30 | 2008-07-29 | Georgetown University | Fluorometric assay for detecting nucleic acid cleavage |
| US5955268A (en) * | 1996-04-26 | 1999-09-21 | Abbott Laboratories | Method and reagent for detecting multiple nucleic acid sequences in a test sample |
| US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US5691145A (en) * | 1996-08-27 | 1997-11-25 | Becton, Dickinson And Company | Detection of nucleic acids using G-quartets |
| US5846726A (en) * | 1997-05-13 | 1998-12-08 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| US5928869A (en) * | 1997-05-30 | 1999-07-27 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| JPH1156398A (ja) * | 1997-08-11 | 1999-03-02 | Hamamatsu Photonics Kk | 二本鎖核酸分解酵素活性の測定方法 |
| US5935791A (en) * | 1997-09-23 | 1999-08-10 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
-
1997
- 1997-11-29 GB GBGB9725237.3A patent/GB9725237D0/en not_active Ceased
-
1998
- 1998-11-27 WO PCT/GB1998/003563 patent/WO1999028501A1/en not_active Ceased
- 1998-11-27 NZ NZ504691A patent/NZ504691A/en not_active IP Right Cessation
- 1998-11-27 DE DE69839235.3T patent/DE69839235T3/de not_active Expired - Lifetime
- 1998-11-27 ES ES98955810T patent/ES2262249T3/es not_active Expired - Lifetime
- 1998-11-27 DE DE69834506T patent/DE69834506T2/de not_active Expired - Lifetime
- 1998-11-27 ES ES06005863T patent/ES2301106T3/es not_active Expired - Lifetime
- 1998-11-27 AU AU12533/99A patent/AU744092B2/en not_active Ceased
- 1998-11-27 GB GB0011451A patent/GB2346695B/en not_active Expired - Fee Related
- 1998-11-27 KR KR1020007005866A patent/KR20010032598A/ko not_active Ceased
- 1998-11-27 JP JP2000523376A patent/JP4535615B2/ja not_active Expired - Fee Related
- 1998-11-27 CA CA2311931A patent/CA2311931C/en not_active Expired - Lifetime
- 1998-11-27 EP EP98955810.1A patent/EP1044283B2/en not_active Expired - Lifetime
- 1998-11-27 AT AT06005863T patent/ATE388245T1/de not_active IP Right Cessation
- 1998-11-27 AT AT98955810T patent/ATE325893T1/de not_active IP Right Cessation
- 1998-11-27 EP EP06005863.3A patent/EP1686191B3/en not_active Expired - Lifetime
- 1998-11-27 GB GB9825925A patent/GB2333596A/en not_active Withdrawn
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