DE69834506T2 - Fluorometrisches verfahren zur überwachung und zum nachweis der amplifizierung von nucleinsäuren - Google Patents
Fluorometrisches verfahren zur überwachung und zum nachweis der amplifizierung von nucleinsäuren Download PDFInfo
- Publication number
- DE69834506T2 DE69834506T2 DE69834506T DE69834506T DE69834506T2 DE 69834506 T2 DE69834506 T2 DE 69834506T2 DE 69834506 T DE69834506 T DE 69834506T DE 69834506 T DE69834506 T DE 69834506T DE 69834506 T2 DE69834506 T2 DE 69834506T2
- Authority
- DE
- Germany
- Prior art keywords
- probe
- molecule
- donor
- amplification
- restriction enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Amplifiers (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9725237 | 1997-11-29 | ||
| GBGB9725237.3A GB9725237D0 (en) | 1997-11-29 | 1997-11-29 | Amplification system |
| PCT/GB1998/003563 WO1999028501A1 (en) | 1997-11-29 | 1998-11-27 | Fluorometric method for monitoring and detecting nucleic acid amplification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69834506D1 DE69834506D1 (de) | 2006-06-14 |
| DE69834506T2 true DE69834506T2 (de) | 2007-04-19 |
Family
ID=10822822
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69834506T Expired - Lifetime DE69834506T2 (de) | 1997-11-29 | 1998-11-27 | Fluorometrisches verfahren zur überwachung und zum nachweis der amplifizierung von nucleinsäuren |
| DE69839235.3T Expired - Lifetime DE69839235T3 (de) | 1997-11-29 | 1998-11-27 | Fluorometrisches Verfahren zur Überwachung und zum Nachweis von Nukleinsäure- Amplifizierung |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69839235.3T Expired - Lifetime DE69839235T3 (de) | 1997-11-29 | 1998-11-27 | Fluorometrisches Verfahren zur Überwachung und zum Nachweis von Nukleinsäure- Amplifizierung |
Country Status (11)
| Country | Link |
|---|---|
| EP (2) | EP1686191B3 (enExample) |
| JP (1) | JP4535615B2 (enExample) |
| KR (1) | KR20010032598A (enExample) |
| AT (2) | ATE388245T1 (enExample) |
| AU (1) | AU744092B2 (enExample) |
| CA (1) | CA2311931C (enExample) |
| DE (2) | DE69834506T2 (enExample) |
| ES (2) | ES2301106T3 (enExample) |
| GB (3) | GB9725237D0 (enExample) |
| NZ (1) | NZ504691A (enExample) |
| WO (1) | WO1999028501A1 (enExample) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9827908D0 (en) * | 1998-12-19 | 1999-02-10 | Univ Manchester | Nucleic acid sequencing method |
| AUPP844899A0 (en) * | 1999-02-01 | 1999-02-25 | University Of Western Australia, The | A method for detecting methylated nucleic acids |
| GB9918237D0 (en) * | 1999-08-04 | 1999-10-06 | Secr Defence | Detection system |
| GB0110501D0 (en) | 2001-04-30 | 2001-06-20 | Secr Defence Brit | Amplification process |
| GB0111275D0 (en) | 2001-05-09 | 2001-06-27 | Secr Defence | Analytical method and kit |
| GB0112868D0 (en) | 2001-05-25 | 2001-07-18 | Secr Defence | Detection system |
| US9261460B2 (en) | 2002-03-12 | 2016-02-16 | Enzo Life Sciences, Inc. | Real-time nucleic acid detection processes and compositions |
| US9353405B2 (en) | 2002-03-12 | 2016-05-31 | Enzo Life Sciences, Inc. | Optimized real time nucleic acid detection processes |
| GB0304832D0 (en) | 2003-03-04 | 2003-04-09 | Secr Defence | Assay method |
| DE10353419B3 (de) * | 2003-11-09 | 2005-06-30 | Epigenomics Ag | Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA-Sequenzen mittels hemimethylierungssensitiver Restriktionsenzyme |
| GB0503172D0 (en) | 2005-02-16 | 2005-03-23 | Enigma Diagnostics Ltd | Detection method |
| GB0517005D0 (en) | 2005-08-19 | 2005-09-28 | Enigma Diagnostics Ltd | Analytical method and kit |
| WO2007060707A2 (en) * | 2005-11-23 | 2007-05-31 | Diasorin Spa | Reagents and method for simultaneous nucleic acid amplification and detection |
| EP1905842A1 (en) * | 2006-09-26 | 2008-04-02 | DiaSorin S.p.A. | Method for detection of mutant alleles combining Real Time PCR and REMS-PCR |
| WO2009135093A2 (en) | 2008-04-30 | 2009-11-05 | Integrated Dna Technologies, Inc. | Rnase-h-based assays utilizing modified rna monomers |
| US8911948B2 (en) | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| AU2012236896A1 (en) | 2011-03-25 | 2013-05-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| JP6440997B2 (ja) * | 2014-08-22 | 2018-12-19 | 国立大学法人大阪大学 | Ｐｃｒ法およびｐｃｒキット |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT728218E (pt) * | 1993-11-12 | 2008-04-08 | Phri Properties Inc | Sondas de hibridação para detecção de ácidos nucleicos, troncos universais, métodos e estojos |
| US5648211A (en) † | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| DE4419566A1 (de) * | 1994-06-03 | 1995-12-07 | Birsner & Grob Biotech Gmbh | Kompartimentiertes Reaktionsgefäß |
| CA2209080C (en) † | 1994-12-30 | 2008-07-29 | Georgetown University | Fluorometric assay for detecting nucleic acid cleavage |
| US5955268A (en) * | 1996-04-26 | 1999-09-21 | Abbott Laboratories | Method and reagent for detecting multiple nucleic acid sequences in a test sample |
| US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US5691145A (en) * | 1996-08-27 | 1997-11-25 | Becton, Dickinson And Company | Detection of nucleic acids using G-quartets |
| US5846726A (en) † | 1997-05-13 | 1998-12-08 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| US5928869A (en) * | 1997-05-30 | 1999-07-27 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
| JPH1156398A (ja) * | 1997-08-11 | 1999-03-02 | Hamamatsu Photonics Kk | 二本鎖核酸分解酵素活性の測定方法 |
| US5935791A (en) * | 1997-09-23 | 1999-08-10 | Becton, Dickinson And Company | Detection of nucleic acids by fluorescence quenching |
-
1997
- 1997-11-29 GB GBGB9725237.3A patent/GB9725237D0/en not_active Ceased
-
1998
- 1998-11-27 ES ES06005863T patent/ES2301106T3/es not_active Expired - Lifetime
- 1998-11-27 DE DE69834506T patent/DE69834506T2/de not_active Expired - Lifetime
- 1998-11-27 WO PCT/GB1998/003563 patent/WO1999028501A1/en not_active Ceased
- 1998-11-27 NZ NZ504691A patent/NZ504691A/en not_active IP Right Cessation
- 1998-11-27 DE DE69839235.3T patent/DE69839235T3/de not_active Expired - Lifetime
- 1998-11-27 AT AT06005863T patent/ATE388245T1/de not_active IP Right Cessation
- 1998-11-27 AT AT98955810T patent/ATE325893T1/de not_active IP Right Cessation
- 1998-11-27 GB GB9825925A patent/GB2333596A/en not_active Withdrawn
- 1998-11-27 GB GB0011451A patent/GB2346695B/en not_active Expired - Fee Related
- 1998-11-27 EP EP06005863.3A patent/EP1686191B3/en not_active Expired - Lifetime
- 1998-11-27 AU AU12533/99A patent/AU744092B2/en not_active Ceased
- 1998-11-27 EP EP98955810.1A patent/EP1044283B2/en not_active Expired - Lifetime
- 1998-11-27 KR KR1020007005866A patent/KR20010032598A/ko not_active Ceased
- 1998-11-27 JP JP2000523376A patent/JP4535615B2/ja not_active Expired - Fee Related
- 1998-11-27 ES ES98955810T patent/ES2262249T3/es not_active Expired - Lifetime
- 1998-11-27 CA CA2311931A patent/CA2311931C/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| ES2262249T3 (es) | 2006-11-16 |
| EP1044283B1 (en) | 2006-05-10 |
| EP1686191A2 (en) | 2006-08-02 |
| ATE388245T1 (de) | 2008-03-15 |
| JP2002507719A (ja) | 2002-03-12 |
| ATE325893T1 (de) | 2006-06-15 |
| GB2346695B (en) | 2002-10-30 |
| KR20010032598A (ko) | 2001-04-25 |
| EP1686191B1 (en) | 2008-03-05 |
| GB9725237D0 (en) | 1998-01-28 |
| DE69839235D1 (de) | 2008-04-17 |
| GB2333596A (en) | 1999-07-28 |
| EP1686191A3 (en) | 2006-09-13 |
| GB9825925D0 (en) | 1999-01-20 |
| CA2311931C (en) | 2011-01-04 |
| DE69839235T2 (de) | 2009-02-26 |
| JP4535615B2 (ja) | 2010-09-01 |
| WO1999028501A1 (en) | 1999-06-10 |
| DE69834506D1 (de) | 2006-06-14 |
| GB0011451D0 (en) | 2000-06-28 |
| AU744092B2 (en) | 2002-02-14 |
| GB2346695A (en) | 2000-08-16 |
| NZ504691A (en) | 2002-02-01 |
| EP1044283B2 (en) | 2015-09-23 |
| EP1044283A1 (en) | 2000-10-18 |
| DE69839235T3 (de) | 2017-02-23 |
| CA2311931A1 (en) | 1999-06-10 |
| AU1253399A (en) | 1999-06-16 |
| EP1686191B3 (en) | 2016-07-20 |
| ES2301106T3 (es) | 2008-06-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8381 | Inventor (new situation) |
Inventor name: LEE, MARTIN, ALAN, SALISBURY WILTSHIRE SP4 0JG, GB |
|
| 8363 | Opposition against the patent |