ES2012940A6 - Un procedimiento para la preparacion de una celula de mamifero capaz de expresar interleukin-2 humana. - Google Patents

Un procedimiento para la preparacion de una celula de mamifero capaz de expresar interleukin-2 humana.

Info

Publication number
ES2012940A6
ES2012940A6 ES8804018A ES8804018A ES2012940A6 ES 2012940 A6 ES2012940 A6 ES 2012940A6 ES 8804018 A ES8804018 A ES 8804018A ES 8804018 A ES8804018 A ES 8804018A ES 2012940 A6 ES2012940 A6 ES 2012940A6
Authority
ES
Spain
Prior art keywords
mammalian cells
human interleukin
enhanced expression
interleukin
high level
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES8804018A
Other languages
English (en)
Inventor
Cullen Bryan Richard Ricard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of ES2012940A6 publication Critical patent/ES2012940A6/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors

Abstract

UN PROCEDIMIENTO PARA LA PREPARACION DE UNA CELULA DE MAMIFERO CAPAZ DE EXPRESAR INTERLEUKIN-2 HUMANA, CUYO PROCEDIMIENTO COMPRENDE TRANSFERIR UNA CELULA DE MAMIFERO CONTENIENDO UN VECTOR RECOMBINANTE QUE COMPRENDE UN VECTOR Y UNA SECUENCIA DE DNA QUE CODIFICA LA INTERLEUKIN-2 HUMANA Y UNA SECUENCIA DE PEPTIDOS SEÑALIZADORA QUE COMPRENDE DE UN GEN QUE CODIFICA LA INTERLEUKIN-2 HUMANA Y UNA SECUENCIA DE PEPTIDOS SEÑALIZADORA EN EL QUE LAS SECUENCIAS NO CODIFICADORAS EN 5'' DEL GEN HAN SIDO SUSTITUIDAS POR SECUENCIAS HETEROLOGAS NO CODIFICADORAS EN 5''. LA INTERLEUKIN-2 HUMANA PUEDE UTILIZARSE COMO COMPUESTO INMUNOMODULADOR PARA EL TRATAMIENTO Y PREVENCION DE ENFERMEDADES.
ES8804018A 1986-05-12 1988-12-30 Un procedimiento para la preparacion de una celula de mamifero capaz de expresar interleukin-2 humana. Expired - Fee Related ES2012940A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/862,082 US4992367A (en) 1986-05-12 1986-05-12 Enhanced expression of human interleukin-2 in mammalian cells

Publications (1)

Publication Number Publication Date
ES2012940A6 true ES2012940A6 (es) 1990-04-16

Family

ID=25337602

Family Applications (2)

Application Number Title Priority Date Filing Date
ES8701394A Expired ES2006476A6 (es) 1986-05-12 1987-05-11 Un procedimiento para obtener interleukin-2 humana
ES8804018A Expired - Fee Related ES2012940A6 (es) 1986-05-12 1988-12-30 Un procedimiento para la preparacion de una celula de mamifero capaz de expresar interleukin-2 humana.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES8701394A Expired ES2006476A6 (es) 1986-05-12 1987-05-11 Un procedimiento para obtener interleukin-2 humana

Country Status (25)

Country Link
US (1) US4992367A (es)
JP (1) JPS62278994A (es)
AR (1) AR241794A1 (es)
AT (1) AT389892B (es)
AU (1) AU603576B2 (es)
BE (1) BE1000717A5 (es)
CH (1) CH675731A5 (es)
DE (1) DE3715808A1 (es)
DK (1) DK234087A (es)
ES (2) ES2006476A6 (es)
FI (1) FI872096A (es)
FR (1) FR2598433B1 (es)
GB (1) GB2190382B (es)
GR (1) GR870727B (es)
HU (1) HU205384B (es)
IL (1) IL82475A0 (es)
IT (1) IT1215478B (es)
LU (1) LU86874A1 (es)
MC (1) MC1821A1 (es)
NL (1) NL8701132A (es)
NO (1) NO871939L (es)
NZ (1) NZ220246A (es)
PT (1) PT84849B (es)
SE (1) SE8701934L (es)
ZA (1) ZA872768B (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR0138654B1 (ko) * 1987-08-26 1998-04-30 프레드릭 에이. 유스티스, 3세 인터루킨-i 저해제 및 이의 제조방법
FR2619719B1 (fr) * 1987-09-01 1991-05-10 Sanofi Sa Procede d'obtention d'interleukine-2 a partir de cellules eucaryotes, vecteurs necessaires a sa mise en oeuvre et lignees cellulaires hautement productrices
FR2636231A1 (fr) * 1988-09-09 1990-03-16 Sanofi Sa Cellules eucaryotes recombinees productrices d'interleukine-1 (beta) mature n-glycosylee, procede et vecteurs necessaires pour leur obtention et procede d'obtention d'interleukine-1 (beta) mature n-glycosylee
WO1990010069A1 (en) * 1989-02-28 1990-09-07 Institut Organicheskogo Sinteza Akademii Nauk Latviiskoi Ssr RECOMBINANT PLASMID DNA pAA 1213-23 AND pAC 262-33 CODING FOR SYNTHESIS OF HUMAN INTERLEUKIN-2, METHOD OF THEIR CONSTRUCTION AND STRAIN OF BACTERIA ESCHERICHIA COLI RRI/pAC 262-33 AS PRODUCER OF HUMAN INTERLEUKIN-2
IL95330A0 (en) * 1989-08-11 1991-06-30 Lilly Co Eli Expression of a functional insect specific toxin gene in mammalian cells
GB9106185D0 (en) * 1991-03-22 1991-05-08 Wellcome Found Biological control agents
US5548075A (en) * 1993-08-30 1996-08-20 Utah State University DNA cassette and transgenic organisms carrying lytic peptide-encoding genes
US5856178A (en) * 1993-08-30 1999-01-05 Utah State University DNA cassettes for expression of lytic peptides in mammalian cells and transgenic organisms containing same
US5641665A (en) * 1994-11-28 1997-06-24 Vical Incorporated Plasmids suitable for IL-2 expression
US5686246A (en) * 1995-08-03 1997-11-11 Kornman; Kenneth S. Detecting genetic predisposition to periodontal disease
EP0855918A4 (en) 1995-10-17 2002-08-14 Univ Wayne State INTERLEUKIN-15 FROM CHICKEN AND USES THEREOF
US6001613A (en) * 1996-05-24 1999-12-14 Board Of Regents Of University Of Nebraska Plasmid bearing a cDNA copy of the genome of bovine viral diarrhea virus, chimeric derivatives thereof, and method of producing an infectious bovine viral diarrhea virus using said plasmid
AUPP655698A0 (en) 1998-10-16 1998-11-05 Commonwealth Scientific And Industrial Research Organisation Delivery system for porcine somatotropin
US20070025958A1 (en) 2000-10-27 2007-02-01 Hadden John W Vaccine immunotherapy
KR20020043448A (ko) * 2000-12-04 2002-06-10 리송알렉스인근 인터루킨-2 재조합 단백질 및 그의 제조방법
US20040071671A1 (en) * 2001-02-20 2004-04-15 Leturcq Didier J. Cell therapy method for the treatment of tumors
EA013944B1 (ru) * 2001-02-20 2010-08-30 Орто-Макнейл Фармасьютикал, Инк. Способ получения неприродной антигенпрезентирующей клеточной линии и применение линии для лечения опухолей

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2814039C3 (de) * 1978-03-31 1981-02-19 Gesellschaft Fuer Biotechnologische Forschung Mbh (Gbf), 3300 Braunschweig Verfahren zur Herstellung von Hybrid-Bakterien
US4405712A (en) * 1981-07-01 1983-09-20 The United States Of America As Represented By The Department Of Health And Human Services LTR-Vectors
DE3377363D1 (en) * 1982-03-31 1988-08-18 Ajinomoto Kk Gene coding for interleukin-2 polypeptide, recombinant dna carrying said gene, cell lines possessing the recombinant dna,and method for producing interleukin-2 using said cells
AU556353B2 (en) * 1982-12-15 1986-10-30 Ajinomoto Co., Inc. Interleukin-2 polypeptide
AU579089B2 (en) * 1983-02-08 1988-11-17 Biogen, Inc. Human interleukin-2-like polypeptides
EP0119621A1 (en) * 1983-03-21 1984-09-26 F. HOFFMANN-LA ROCHE & CO. Aktiengesellschaft Interleukin-2
WO1985000817A1 (en) * 1983-08-10 1985-02-28 Amgen Microbial expression of interleukin ii
GB8327880D0 (en) * 1983-10-18 1983-11-16 Ajinomoto Kk Saccharomyces cerevisiae
JPS61500586A (ja) * 1983-11-14 1986-04-03 チロン　コ−ポレイシヨン インタ−ロイキン−２産生のための方法及び組成物
FR2559782B1 (fr) * 1984-02-16 1986-07-18 Transgene Sa Vecteur d'expression dans les levures de l'interleukine-2, levures transformees et procede de preparation de l'interleukine-2
DE3574731D1 (de) * 1984-05-08 1990-01-18 Genetics Inst Ein menschlicher t-zellwachstumsfaktor.
DE3419995A1 (de) * 1984-05-29 1985-12-05 Hoechst Ag, 6230 Frankfurt Gentechnologisches verfahren zur herstellung von human-interleukin-2 und mittel zur durchfuehrung dieses verfahrens
EP0172619A1 (en) * 1984-06-20 1986-02-26 Takeda Chemical Industries, Ltd. Novel transformant and use thereof
CA1275954C (en) * 1984-08-31 1990-11-06 Hing Cheug Wong 3'-expression enhancing fragments and method
FR2583770B1 (fr) * 1985-06-21 1988-08-19 Transgene Sa Expression de l'il-2 humaine dans les cellules de mammiferes par un poxvirus recombine
FR2619719B1 (fr) * 1987-09-01 1991-05-10 Sanofi Sa Procede d'obtention d'interleukine-2 a partir de cellules eucaryotes, vecteurs necessaires a sa mise en oeuvre et lignees cellulaires hautement productrices

Also Published As

Publication number Publication date
DE3715808A1 (de) 1987-12-10
AU7270387A (en) 1987-11-19
JPS62278994A (ja) 1987-12-03
BE1000717A5 (fr) 1989-03-21
ATA263787A (de) 1989-07-15
FI872096A0 (fi) 1987-05-12
FR2598433A1 (fr) 1987-11-13
NO871939D0 (no) 1987-05-11
PT84849B (pt) 1990-02-08
CH675731A5 (es) 1990-10-31
GB2190382B (en) 1990-08-15
HU205384B (en) 1992-04-28
DK234087A (da) 1987-11-13
NO871939L (no) 1987-11-13
FR2598433B1 (fr) 1990-09-21
AU603576B2 (en) 1990-11-22
HUT45558A (en) 1988-07-28
US4992367A (en) 1991-02-12
FI872096A (fi) 1987-11-13
MC1821A1 (fr) 1988-03-18
GB2190382A (en) 1987-11-18
NZ220246A (en) 1990-07-26
AT389892B (de) 1990-02-12
IL82475A0 (en) 1987-11-30
GB8711072D0 (en) 1987-06-17
NL8701132A (nl) 1987-12-01
SE8701934L (sv) 1987-11-13
GR870727B (en) 1987-09-21
IT1215478B (it) 1990-02-14
PT84849A (pt) 1987-06-01
ES2006476A6 (es) 1989-05-01
LU86874A1 (fr) 1988-06-13
DK234087D0 (da) 1987-05-08
ZA872768B (en) 1988-04-27
AR241794A1 (es) 1992-12-30
IT8720479A0 (it) 1987-05-12
SE8701934D0 (sv) 1987-05-11

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20000301