EP0779980A1 - Procede d'essai toxicologique de matieres, en particulier de matieres plastiques, et dispositif servant a la mise en uvre du procede - Google Patents

Procede d'essai toxicologique de matieres, en particulier de matieres plastiques, et dispositif servant a la mise en uvre du procede

Info

Publication number
EP0779980A1
EP0779980A1 EP95932678A EP95932678A EP0779980A1 EP 0779980 A1 EP0779980 A1 EP 0779980A1 EP 95932678 A EP95932678 A EP 95932678A EP 95932678 A EP95932678 A EP 95932678A EP 0779980 A1 EP0779980 A1 EP 0779980A1
Authority
EP
European Patent Office
Prior art keywords
cells
arachidonic acid
test
tested
toxicity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP95932678A
Other languages
German (de)
English (en)
Inventor
Hans-Peter KLÖCKING
Renate KLÖCKING
Kathrin Hoffmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dr Fritz Nerbe Nachfolger Juergen Nerbe oHG
Original Assignee
Dr Fritz Nerbe Nachfolger Juergen Nerbe oHG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dr Fritz Nerbe Nachfolger Juergen Nerbe oHG filed Critical Dr Fritz Nerbe Nachfolger Juergen Nerbe oHG
Publication of EP0779980A1 publication Critical patent/EP0779980A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/60—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances involving radioactive labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un procédé d'essai toxicologique de matières, en particulier de matières plastiques, ledit procédé consistant à mettre des cellules en incubation, dans des conditions de réaction définies, avec la matière à tester dans des préparations liquides, puis à déterminer le degré de toxicité du matériau sur la base de la réaction des cellules. Ce procédé est caractérisé en ce que l'on utilise des cellules dont les membranes cellulaires contiennent de l'acide arachidonique comme composant principal, l'acide arachidonique [3H] ayant été introduit au préalable dans ces membranes. Les cellules marquées sont mises en incubation dans une préparation test en présence de la matière à tester et dans une préparation témoin sans matière à tester. La préparation test et la préparation témoin sont ajustées de façon à contenir un nombre identique de cellules. La radioactivité est déterminée à un moment défini dans l'excédent sans cellule des préparations. La différence entre les valeurs mesurées pour la préparation test et la préparation témoin permet de déduire des indications concernant la toxicité de la matière testée.
EP95932678A 1994-09-10 1995-09-07 Procede d'essai toxicologique de matieres, en particulier de matieres plastiques, et dispositif servant a la mise en uvre du procede Withdrawn EP0779980A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19944432326 DE4432326C2 (de) 1994-09-10 1994-09-10 Verfahren zur toxikologischen Prüfung von Werkstoffen, insbesondere Kunststoffen, und Vorrichtung zur Durchführung des Verfahrens
DE4432326 1994-09-10
PCT/EP1995/003508 WO1996007904A1 (fr) 1994-09-10 1995-09-07 Procede d'essai toxicologique de matieres, en particulier de matieres plastiques, et dispositif servant a la mise en ×uvre du procede

Publications (1)

Publication Number Publication Date
EP0779980A1 true EP0779980A1 (fr) 1997-06-25

Family

ID=6527943

Family Applications (1)

Application Number Title Priority Date Filing Date
EP95932678A Withdrawn EP0779980A1 (fr) 1994-09-10 1995-09-07 Procede d'essai toxicologique de matieres, en particulier de matieres plastiques, et dispositif servant a la mise en uvre du procede

Country Status (3)

Country Link
EP (1) EP0779980A1 (fr)
DE (1) DE4432326C2 (fr)
WO (1) WO1996007904A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19751581C2 (de) * 1997-11-20 2002-05-08 Thorsten Bechert Verfahren und Vorrichtung zur Prüfung von Materialien hinsichtlich ihrer potentiellen antimikrobiellen Wirksamkeit und der Adhäsion auf ihrer Oberfläche
DE19825909C1 (de) * 1998-06-10 1999-11-25 Graffinity Pharm Design Gmbh Reaktorträger mit mehreren Mikroprobenaufnahmekammern
DE19856229C1 (de) * 1998-12-04 2000-08-31 Deutsches Krebsforsch Verfahren zur Bestimmung von biologischen Langzeitwirkungen von Stoffen und Kit zur Durchführung des Verfahrens
DE10312505B4 (de) * 2003-03-15 2007-07-19 Pauli, Wilfried, Dr. Kultivierungssysteme zur Gewinnung von Langzeit-Reinkulturen wasserlebender Kleinorganismen

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4246339A (en) * 1978-11-01 1981-01-20 Millipore Corporation Test device
AU658079B2 (en) * 1992-09-28 1995-03-30 Becton Dickinson & Company Cell culture insert

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9607904A1 *

Also Published As

Publication number Publication date
DE4432326A1 (de) 1996-03-14
DE4432326C2 (de) 1997-07-17
WO1996007904A1 (fr) 1996-03-14

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