EA201171206A1 - Способ мультипраймерной амплификации для штрихового кодирования целевых нуклеиновых кислот - Google Patents

Способ мультипраймерной амплификации для штрихового кодирования целевых нуклеиновых кислот

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Publication number
EA201171206A1
EA201171206A1 EA201171206A EA201171206A EA201171206A1 EA 201171206 A1 EA201171206 A1 EA 201171206A1 EA 201171206 A EA201171206 A EA 201171206A EA 201171206 A EA201171206 A EA 201171206A EA 201171206 A1 EA201171206 A1 EA 201171206A1
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Prior art keywords
input lines
multipreimeral
amplification
chambers
nucleic acids
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EA201171206A
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English (en)
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EA023190B9 (ru
EA023190B1 (ru
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Эндрю Мэй
Пэйлинь Чэнь
Цзюнь ВАН
Фиона Капер
Меган Андерсон
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Флуидигм Корпорейшн
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Publication of EA201171206A1 publication Critical patent/EA201171206A1/ru
Publication of EA023190B1 publication Critical patent/EA023190B1/ru
Publication of EA023190B9 publication Critical patent/EA023190B9/ru

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

В определенных вариантах осуществления данное изобретение обеспечивает способы амплификации, в которых нуклеотидную метку(и) и, факультативно, штрихкодовую нуклеотидную последовательность добавляют к целевым нуклеотидным последовательностям. В других вариантах осуществления данное изобретение обеспечивает микрофлюидное устройство, которое включает множество первых линий ввода и множество вторых линий ввода. Микрофлюидное устройство также включает множество наборов первых камер и множество наборов вторых камер. Каждый набор первых камер находится в жидкостной связи с одним из множества первых линий ввода. Каждый набор вторых камер находится в жидкостной связи с одним из множества вторых линий ввода. Микрофлюидное устройство дополнительно включает множество первых плунжерных пар в жидкостной связи с первой частью множества вторых линий ввода и множество вторых плунжерных пар в жидкостной связи с второй частью множества вторых линий ввода.
EA201171206A 2009-04-02 2010-04-02 Способ мультипраймерной амплификации для штрихового кодирования целевых нуклеиновых кислот EA023190B9 (ru)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US16618109P 2009-04-02 2009-04-02
US16610509P 2009-04-02 2009-04-02
US18632709P 2009-06-11 2009-06-11
US30590710P 2010-02-18 2010-02-18
PCT/US2010/029854 WO2010115154A1 (en) 2009-04-02 2010-04-02 Multi-primer amplification method for barcoding of target nucleic acids

Publications (3)

Publication Number Publication Date
EA201171206A1 true EA201171206A1 (ru) 2012-07-30
EA023190B1 EA023190B1 (ru) 2016-05-31
EA023190B9 EA023190B9 (ru) 2018-03-30

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EA201171206A EA023190B9 (ru) 2009-04-02 2010-04-02 Способ мультипраймерной амплификации для штрихового кодирования целевых нуклеиновых кислот

Country Status (13)

Country Link
US (6) US8691509B2 (ru)
EP (2) EP2789694A1 (ru)
JP (2) JP5985390B2 (ru)
KR (2) KR101829182B1 (ru)
CN (3) CN103952482A (ru)
AU (2) AU2010232439C1 (ru)
CA (2) CA2757560C (ru)
DK (1) DK2414547T3 (ru)
EA (1) EA023190B9 (ru)
HK (2) HK1170543A1 (ru)
IL (1) IL215462A (ru)
SG (2) SG174617A1 (ru)
WO (1) WO2010115154A1 (ru)

Families Citing this family (289)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2552262C (en) 2004-02-02 2016-04-19 The University Of British Columbia Scodaphoresis and methods and apparatus for moving and concentrating particles
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
US8529744B2 (en) 2004-02-02 2013-09-10 Boreal Genomics Corp. Enrichment of nucleic acid targets
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US20090317798A1 (en) * 2005-06-02 2009-12-24 Heid Christian A Analysis using microfluidic partitioning devices
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US8781532B2 (en) * 2005-09-19 2014-07-15 Google Inc. Customized data retrieval applications for mobile devices providing interpretation of markup language data
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP2481815B1 (en) 2006-05-11 2016-01-27 Raindance Technologies, Inc. Microfluidic devices
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
EP2238434A4 (en) 2008-02-01 2011-06-22 Univ British Columbia METHODS AND APPARATUS FOR INTRODUCING AND RECOVERING PARTICLES
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
EP2326732A4 (en) * 2008-08-26 2012-11-14 Fluidigm Corp TEST METHODS FOR INCREASED SURPLUS OF SAMPLES AND / OR TARGETS
WO2010033200A2 (en) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
KR20110106922A (ko) 2009-01-13 2011-09-29 플루이다임 코포레이션 단일 세포 핵산 분석
US8450063B2 (en) 2009-01-28 2013-05-28 Fluidigm Corporation Determination of copy number differences by amplification
US8691509B2 (en) 2009-04-02 2014-04-08 Fluidigm Corporation Multi-primer amplification method for barcoding of target nucleic acids
AU2010242073C1 (en) 2009-04-30 2015-12-24 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
GB2487341A (en) * 2009-11-02 2012-07-18 Nugen Technologies Inc Compositions and methods for targeted nucleic acid sequence selection and amplification
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US10351905B2 (en) * 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
WO2011100604A2 (en) 2010-02-12 2011-08-18 Raindance Technologies, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
EP2854057B1 (en) 2010-05-18 2018-03-07 Natera, Inc. Methods for non-invasive pre-natal ploidy calling
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
CN101921874B (zh) * 2010-06-30 2013-09-11 深圳华大基因科技有限公司 基于Solexa测序法的检测人类乳头瘤病毒的方法
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
SG189839A1 (en) 2010-10-04 2013-06-28 Genapsys Inc Systems and methods for automated reusable parallel biological reactions
GB2497912B (en) 2010-10-08 2014-06-04 Harvard College High-throughput single cell barcoding
EP2638157B1 (en) 2010-11-12 2015-07-22 Gen9, Inc. Methods and devices for nucleic acids synthesis
EP4039363A1 (en) 2010-11-12 2022-08-10 Gen9, Inc. Protein arrays and methods of using and making the same
US9163281B2 (en) * 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
US9952237B2 (en) * 2011-01-28 2018-04-24 Quanterix Corporation Systems, devices, and methods for ultra-sensitive detection of molecules or particles
US9738930B2 (en) 2011-01-28 2017-08-22 The Broad Institute, Inc. Paired end bead amplification and high throughput sequencing
JP6153874B2 (ja) 2011-02-09 2017-06-28 ナテラ， インコーポレイテッド 非侵襲的出生前倍数性呼び出しのための方法
WO2012109600A2 (en) 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
CN110016499B (zh) 2011-04-15 2023-11-14 约翰·霍普金斯大学 安全测序系统
JP5998203B2 (ja) 2011-04-28 2016-09-28 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 試料に関連するポリヌクレオチドの同定
TWI465572B (zh) * 2011-05-19 2014-12-21 Univ Chang Gung Method, composition and system of amplification and detection of target microbial DNA
SG194745A1 (en) * 2011-05-20 2013-12-30 Fluidigm Corp Nucleic acid encoding reactions
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
EP3709018A1 (en) 2011-06-02 2020-09-16 Bio-Rad Laboratories, Inc. Microfluidic apparatus for identifying components of a chemical reaction
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
US20140371083A1 (en) * 2011-07-14 2014-12-18 Celula, Inc. Systems, apparatus and methods for biochemical analysis
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
WO2013022961A1 (en) 2011-08-08 2013-02-14 3The Broad Institute Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence
EP2559774A1 (en) 2011-08-17 2013-02-20 Roche Diagniostics GmbH Improved method for amplification of target nucleic acids using a multi-primer approach
LT2944693T (lt) 2011-08-26 2019-08-26 Gen9, Inc. Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
GB2497838A (en) 2011-10-19 2013-06-26 Nugen Technologies Inc Compositions and methods for directional nucleic acid amplification and sequencing
EP2785868B1 (en) 2011-12-01 2017-04-12 Genapsys Inc. Systems and methods for high efficiency electronic sequencing and detection
WO2013104994A2 (en) 2012-01-13 2013-07-18 The University Of British Columbia Multiple arm apparatus and methods for separation of particles
EP4372084A3 (en) 2012-01-26 2024-08-14 Tecan Genomics, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
LT3363901T (lt) 2012-02-17 2021-04-12 Fred Hutchinson Cancer Research Center Kompozicijos ir būdai, skirti tiksliam mutacijų nustatymui
EP3309262B1 (en) * 2012-02-24 2019-09-25 Bio-Rad Laboratories, Inc. Labeling and sample preparation for sequencing
CN104364392B (zh) 2012-02-27 2018-05-25 赛卢拉研究公司 用于分子计数的组合物和试剂盒
EP3305918B1 (en) 2012-03-05 2020-06-03 President and Fellows of Harvard College Methods for epigenetic sequencing
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
EP4001427A1 (en) 2012-04-24 2022-05-25 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US9512477B2 (en) 2012-05-04 2016-12-06 Boreal Genomics Inc. Biomarker anaylsis using scodaphoresis
US20130309667A1 (en) * 2012-05-15 2013-11-21 Anthony P. Shuber Primers for analyzing methylated sequences and methods of use thereof
CA2874413A1 (en) 2012-05-21 2013-11-28 The Scripps Research Institute Methods of sample preparation
JP2015519900A (ja) 2012-05-21 2015-07-16 フリューダイム・コーポレイション 粒子集団の単粒子解析方法及び単粒子単離方法
AU2013269536B2 (en) * 2012-06-01 2018-11-08 European Molecular Biology Laboratory High-capacity storage of digital information in DNA
CN104619894B (zh) 2012-06-18 2017-06-06 纽亘技术公司 用于非期望核酸序列的阴性选择的组合物和方法
CN104685116A (zh) 2012-06-25 2015-06-03 Gen9股份有限公司 用于核酸组装和高通量测序的方法
CN104781415A (zh) * 2012-06-28 2015-07-15 卡尔德拉健康有限责任公司 用于诊断前列腺癌的靶向RNA-Seq的方法和材料
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
KR101890466B1 (ko) * 2012-07-24 2018-08-21 내테라, 인코포레이티드 고도의 다중 ｐｃｒ 방법 및 조성물
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US20140155295A1 (en) 2012-08-14 2014-06-05 10X Technologies, Inc. Capsule array devices and methods of use
US20150376609A1 (en) * 2014-06-26 2015-12-31 10X Genomics, Inc. Methods of Analyzing Nucleic Acids from Individual Cells or Cell Populations
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
GB2521570B (en) * 2012-09-24 2021-01-06 Irepertoire Inc Multiplex pyrosequencing using non-interfering noise cancelling polynucleotide identification tags
GB201217888D0 (en) * 2012-10-05 2012-11-21 Univ Leuven Kath High-throughput genotyping by sequencing of single cell
USRE50065E1 (en) 2012-10-17 2024-07-30 10X Genomics Sweden Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
ES2701742T3 (es) 2012-10-29 2019-02-25 Univ Johns Hopkins Prueba de Papanicolaou para cánceres de ovario y de endometrio
CN104903466B (zh) 2012-11-05 2016-11-23 鲁比康基因组学公司 条形编码核酸
WO2014093330A1 (en) 2012-12-10 2014-06-19 Clearfork Bioscience, Inc. Methods for targeted genomic analysis
EP2931919B1 (en) 2012-12-14 2019-02-20 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014106076A2 (en) * 2012-12-28 2014-07-03 Quest Diagnostics Investments Incorporated Universal sanger sequencing from next-gen sequencing amplicons
WO2014100866A1 (en) 2012-12-28 2014-07-03 Fleury S.A. Method for complete tracking of a set of biological samples containing dna or rna through molecular barcode identification during laboratorial workflow and kit for collecting biological samples containing dna or rna
AU2014205110A1 (en) 2013-01-13 2015-08-27 Unitaq Bio Methods and compositions for PCR using blocked and universal primers
CN108753766A (zh) 2013-02-08 2018-11-06 10X基因组学有限公司 多核苷酸条形码生成
CA2902207A1 (en) * 2013-02-21 2014-08-28 Toma Biosciences, Inc. Methods, compositions, and kits for nucleic acid analysis
WO2014152822A2 (en) * 2013-03-14 2014-09-25 Quest Diagnostics Investments Incorporated Method for detecting cystic fibrosis
US9340835B2 (en) 2013-03-15 2016-05-17 Boreal Genomics Corp. Method for separating homoduplexed and heteroduplexed nucleic acids
US20140274738A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
WO2014152625A1 (en) 2013-03-15 2014-09-25 Genapsys, Inc. Systems and methods for biological analysis
DK3013984T3 (da) 2013-06-25 2023-06-06 Prognosys Biosciences Inc Metode til bestemmelse af spatiale mønstre i biologiske targets i en prøve
KR102436171B1 (ko) 2013-06-27 2022-08-24 10엑스 제노믹스, 인크. 샘플 처리를 위한 조성물 및 방법
GB2546833B (en) 2013-08-28 2018-04-18 Cellular Res Inc Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
EP3055676A1 (en) 2013-10-07 2016-08-17 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
EP3065712A4 (en) 2013-11-08 2017-06-21 President and Fellows of Harvard College Microparticles, methods for their preparation and use
CA2929596C (en) 2013-11-13 2022-07-05 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
WO2015089238A1 (en) 2013-12-11 2015-06-18 Genapsys, Inc. Systems and methods for biological analysis and computation
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
WO2015119941A2 (en) 2014-02-04 2015-08-13 Igenomx International Genomics Corporation Genome fractioning
CN106414770A (zh) * 2014-02-27 2017-02-15 艾格诺姆克斯国际基因组学公司 用于分析体细胞可动因子的方法及其用途
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
CA2943624A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
WO2015161054A2 (en) 2014-04-18 2015-10-22 Genapsys, Inc. Methods and systems for nucleic acid amplification
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
CN106460070B (zh) 2014-04-21 2021-10-08 纳特拉公司 检测染色体片段中的突变和倍性
CA2946144A1 (en) * 2014-04-21 2015-10-29 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
WO2015187953A2 (en) * 2014-06-04 2015-12-10 Quest Diagnostics Investments Incorporated Method for direct microbial identification
EP4235677A3 (en) 2014-06-26 2023-11-22 10X Genomics, Inc. Processes and systems for nucleic acid sequence assembly
EP3169793B1 (en) * 2014-07-16 2022-07-06 Tangen Biosciences Inc. Isothermal methods for amplifying nucleic acid samples
JP6803327B2 (ja) 2014-08-06 2020-12-23 ニューゲン テクノロジーズ， インコーポレイテッド 標的化されたシークエンシングからのデジタル測定値
EP3191604B1 (en) 2014-09-09 2021-04-14 Igenomx International Genomics Corporation Methods and compositions for rapid nucleic acid library preparation
US11408024B2 (en) 2014-09-10 2022-08-09 Molecular Loop Biosciences, Inc. Methods for selectively suppressing non-target sequences
EP3212807B1 (en) 2014-10-29 2020-09-02 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US11559801B2 (en) 2014-11-03 2023-01-24 Tangen Biosciences, Inc. Apparatus and method for cell, spore, or virus capture and disruption
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
KR102417849B1 (ko) * 2014-11-21 2022-07-07 나노스트링 테크놀로지스, 인크. 무효소 및 무증폭 시퀀싱
CA3010579A1 (en) 2015-01-06 2016-07-14 Good Start Genetics, Inc. Screening for structural variants
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
WO2016115273A1 (en) 2015-01-13 2016-07-21 10X Genomics, Inc. Systems and methods for visualizing structural variation and phasing information
US20180010120A1 (en) * 2015-01-16 2018-01-11 Seqwell, Inc. Normalized iterative barcoding and sequencing of dna collections
WO2016115457A1 (en) * 2015-01-16 2016-07-21 California Institute Of Technology Methods and devices for micro-isolation, extraction, and/or analysis of microscale components in an array
AU2016219480B2 (en) 2015-02-09 2021-11-11 10X Genomics, Inc. Systems and methods for determining structural variation and phasing using variant call data
ES2975332T3 (es) 2015-02-19 2024-07-04 Becton Dickinson Co Análisis unicelular de alto rendimiento que combina información proteómica y genómica
AU2016222719B2 (en) 2015-02-24 2022-03-31 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
US10697000B2 (en) 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
US10968536B2 (en) 2015-02-25 2021-04-06 Jumpcode Genomics, Inc. Methods and compositions for sequencing
EP3262214B1 (en) * 2015-02-27 2023-10-25 Standard BioTools Inc. Microfluidic device
EP3262192B1 (en) 2015-02-27 2020-09-16 Becton, Dickinson and Company Spatially addressable molecular barcoding
JP7508191B2 (ja) 2015-03-30 2024-07-01 ベクトン・ディキンソン・アンド・カンパニー コンビナトリアルバーコーディングのための方法および組成物
FI3901281T3 (fi) 2015-04-10 2023-01-31 Biologisten näytteiden spatiaalisesti eroteltu moninkertainen nukleiinihappoanalyysi
US11746367B2 (en) 2015-04-17 2023-09-05 President And Fellows Of Harvard College Barcoding systems and methods for gene sequencing and other applications
US10385387B2 (en) 2015-04-20 2019-08-20 Pacific Biosciences Of California, Inc. Methods for selectively amplifying and tagging nucleic acids
US11390914B2 (en) 2015-04-23 2022-07-19 Becton, Dickinson And Company Methods and compositions for whole transcriptome amplification
EP4428863A2 (en) 2015-05-11 2024-09-11 Natera, Inc. Methods and compositions for determining ploidy
US11130986B2 (en) 2015-05-20 2021-09-28 Quantum-Si Incorporated Method for isolating target nucleic acid using heteroduplex binding proteins
WO2016196229A1 (en) 2015-06-01 2016-12-08 Cellular Research, Inc. Methods for rna quantification
AU2016289369B2 (en) * 2015-07-07 2020-07-02 Pillar Biosciences Inc. Method for reducing primer-dimer amplification
WO2017027653A1 (en) 2015-08-11 2017-02-16 The Johns Hopkins University Assaying ovarian cyst fluid
ES2745694T3 (es) 2015-09-11 2020-03-03 Cellular Res Inc Métodos y composiciones para normalización de biblioteca de ácido nucleico
AU2016338907B2 (en) 2015-10-13 2022-07-07 President And Fellows Of Harvard College Systems and methods for making and using gel microspheres
WO2017079593A1 (en) * 2015-11-04 2017-05-11 Atreca, Inc. Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
MX2018005858A (es) 2015-11-11 2019-02-20 Resolution Bioscience Inc Construccion de alta eficacia de bibliotecas de adn.
CN115369161A (zh) 2015-12-04 2022-11-22 10X 基因组学有限公司 用于核酸分析的方法和组合物
WO2017106777A1 (en) 2015-12-16 2017-06-22 Fluidigm Corporation High-level multiplex amplification
WO2017138984A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Systems, methods, and media for de novo assembly of whole genome sequence data
US11339427B2 (en) 2016-02-12 2022-05-24 Jumpcode Genomics, Inc. Method for target specific RNA transcription of DNA sequences
JP5997407B1 (ja) * 2016-04-21 2016-09-28 日鉄住金環境株式会社 多項目増幅手法
ES2956757T3 (es) 2016-05-02 2023-12-27 Becton Dickinson Co Codificación con códigos de barras moleculares precisa
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
EP3465502B1 (en) 2016-05-26 2024-04-10 Becton, Dickinson and Company Molecular label counting adjustment methods
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
CA3027423A1 (en) * 2016-07-14 2018-01-18 Fluidigm Corporation Single-cell transcript sequencing
CN116397014A (zh) 2016-07-20 2023-07-07 测序健康公司 用于核酸测序的系统和方法
WO2018039463A1 (en) 2016-08-25 2018-03-01 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in dna samples
KR102363716B1 (ko) 2016-09-26 2022-02-18 셀룰러 리서치, 인크. 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정
US11485996B2 (en) 2016-10-04 2022-11-01 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
US11667951B2 (en) 2016-10-24 2023-06-06 Geneinfosec, Inc. Concealing information present within nucleic acids
ES2980967T3 (es) 2016-11-08 2024-10-03 Becton Dickinson And Company Métodos para la clasificación de perfiles de expresión
KR20190077061A (ko) 2016-11-08 2019-07-02 셀룰러 리서치, 인크. 세포 표지 분류 방법
CN110177621B (zh) * 2016-11-17 2022-07-08 康比纳提公司 用于核酸分析和定量的方法和系统
US10415080B2 (en) 2016-11-21 2019-09-17 Nanostring Technologies, Inc. Chemical compositions and methods of using same
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
US10813383B2 (en) * 2016-12-12 2020-10-27 R.J. Reynolds Tobacco Company Dehydration of tobacco and tobacco-derived materials
US11371090B2 (en) 2016-12-12 2022-06-28 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
JP7104048B2 (ja) 2017-01-13 2022-07-20 セルラー リサーチ， インコーポレイテッド 流体チャネルの親水性コーティング
WO2018140966A1 (en) 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
WO2018144240A1 (en) 2017-02-01 2018-08-09 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
EP3357575B1 (en) * 2017-02-06 2021-03-17 H. Hoffnabb-La Roche Ag Sealable microfluidic chip and method for thermocycling
US20210130879A1 (en) * 2017-03-24 2021-05-06 The Johns Hopkins University Strand-specific detection of bisulfite-converted duplexes
WO2018183942A1 (en) 2017-03-31 2018-10-04 Grail, Inc. Improved library preparation and use thereof for sequencing-based error correction and/or variant identification
CN106835292B (zh) * 2017-04-05 2019-04-09 北京泛生子基因科技有限公司 一步法快速构建扩增子文库的方法
CN110870018A (zh) 2017-05-19 2020-03-06 10X基因组学有限公司 用于分析数据集的系统和方法
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP4230746A3 (en) 2017-05-26 2023-11-01 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP3635135A1 (en) 2017-06-05 2020-04-15 Becton, Dickinson and Company Sample indexing for single cells
US11174511B2 (en) * 2017-07-24 2021-11-16 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture
CN111566224A (zh) 2017-09-21 2020-08-21 吉纳普赛斯股份有限公司 用于核酸测序的系统和方法
CN115506034A (zh) * 2017-09-25 2022-12-23 普莱克斯姆公司 寡核苷酸编码的化学文库
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
WO2019084517A1 (en) * 2017-10-26 2019-05-02 Essenlix Corporation SYSTEM AND METHODS FOR IMAGE-BASED ANALYSIS USING MACHINE LEARNING AND CROF
CN111051523B (zh) 2017-11-15 2024-03-19 10X基因组学有限公司 功能化凝胶珠
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
KR102371222B1 (ko) * 2017-11-29 2022-03-07 주식회사 파나진 표적핵산 증폭방법 및 표적핵산 증폭용 조성물
US12084720B2 (en) 2017-12-14 2024-09-10 Natera, Inc. Assessing graft suitability for transplantation
WO2019126209A1 (en) 2017-12-19 2019-06-27 Cellular Research, Inc. Particles associated with oligonucleotides
GB2589159B (en) 2017-12-29 2023-04-05 Clear Labs Inc Nucleic acid sequencing apparatus
CA3093451A1 (en) * 2018-03-08 2019-09-12 Pathogendx, Inc. Microarray based multiplex pathogen analysis and uses thereof
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
CA3090426A1 (en) 2018-04-14 2019-10-17 Natera, Inc. Methods for cancer detection and monitoring by means of personalized detection of circulating tumor dna
EP3560593B1 (en) * 2018-04-25 2024-06-05 OPTOLANE Technologies Inc. Cartridge for digital real-time pcr
US11365409B2 (en) 2018-05-03 2022-06-21 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
CA3097976A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
SG11202011274YA (en) 2018-05-14 2020-12-30 Nanostring Technologies Inc Chemical compositions and methods of using same
US11525159B2 (en) 2018-07-03 2022-12-13 Natera, Inc. Methods for detection of donor-derived cell-free DNA
CN116064754A (zh) * 2018-08-28 2023-05-05 深圳联合医学科技有限公司 减少交叉污染的扩增子测序文库的构建方法
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
JP2022511398A (ja) 2018-10-01 2022-01-31 ベクトン・ディキンソン・アンド・カンパニー ５’転写物配列の決定
JP2022506546A (ja) 2018-11-08 2022-01-17 ベクトン・ディキンソン・アンド・カンパニー ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析
EP3894587A1 (en) 2018-12-10 2021-10-20 10X Genomics, Inc. Resolving spatial arrays by proximity-based deconvolution
CN113195717A (zh) 2018-12-13 2021-07-30 贝克顿迪金森公司 单细胞全转录组分析中的选择性延伸
EP3666904A1 (en) 2018-12-14 2020-06-17 Lexogen GmbH Nucleic acid amplification and identification method
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11371076B2 (en) 2019-01-16 2022-06-28 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
CN113574178A (zh) 2019-01-23 2021-10-29 贝克顿迪金森公司 与抗体关联的寡核苷酸
EP3924506A1 (en) 2019-02-14 2021-12-22 Becton Dickinson and Company Hybrid targeted and whole transcriptome amplification
CN113557311A (zh) * 2019-03-13 2021-10-26 大日本住友制药株式会社 移植用神经视网膜的品质评价方法及移植用神经视网膜片
WO2020214642A1 (en) 2019-04-19 2020-10-22 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US11939622B2 (en) 2019-07-22 2024-03-26 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
KR20220101108A (ko) 2019-10-29 2022-07-19 퀀텀-에스아이 인코포레이티드 유체의 연동 펌핑 및 연관 방법, 시스템, 및 디바이스
WO2021092386A1 (en) 2019-11-08 2021-05-14 Becton Dickinson And Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
WO2021118435A1 (en) * 2019-12-09 2021-06-17 Laboratorios Maymó S.A. Rapid amplification and genotyping of nucleic acid sequences
EP4424843A3 (en) 2019-12-23 2024-09-25 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
WO2021133842A1 (en) 2019-12-23 2021-07-01 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
EP4090763A1 (en) 2020-01-13 2022-11-23 Becton Dickinson and Company Methods and compositions for quantitation of proteins and rna
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
CN115349128A (zh) 2020-02-13 2022-11-15 齐默尔根公司 宏基因组文库和天然产物发现平台
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
CN111394432B (zh) * 2020-03-27 2023-03-24 深圳闪量科技有限公司 基于通用探针芯片的多重定量pcr检测系统
CN115916999A (zh) 2020-04-22 2023-04-04 10X基因组学有限公司 用于使用靶向rna耗竭进行空间分析的方法
CA3176541A1 (en) * 2020-04-24 2021-10-28 Oliver Gediminas WACHTER Single step sample preparation for next generation sequencing
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
EP4414459A3 (en) 2020-05-22 2024-09-18 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4165207B1 (en) * 2020-06-10 2024-09-25 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
EP4450639A2 (en) 2020-06-25 2024-10-23 10X Genomics, Inc. Spatial analysis of dna methylation
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
KR102526524B1 (ko) 2020-09-02 2023-04-27 한국표준과학연구원 전장유전체 증폭을 위한 인간 사스-코로나바이러스-2 범용 프라이머 세트 및 이를 이용한 진단 키트
US20240011020A1 (en) * 2020-09-08 2024-01-11 Seqwell, Inc Sequencing oligonucleotides and methods of use thereof
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
KR102702774B1 (ko) * 2020-12-09 2024-09-05 고려대학교 산학협력단 위치기반 단일 세포 유전자 발현 분석 방법 및 장치
RU2752840C1 (ru) * 2020-12-17 2021-08-09 Общество с ограниченной ответственностью «ДНК-дисплей» Способ штрихкодирования ампликонов при подготовке таргетных библиотек ДНК к массовому параллельному секвенированию
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US20240279643A1 (en) * 2020-12-25 2024-08-22 Intelligent Healthcare Technology Limited Biochip for spatial transcriptomic analysis, manufacturing method therefor and application thereof
WO2022178267A2 (en) 2021-02-19 2022-08-25 10X Genomics, Inc. Modular assay support devices
EP4347879A1 (en) 2021-06-03 2024-04-10 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
CN113249505A (zh) * 2021-06-11 2021-08-13 内蒙古农业大学 鉴定大肠杆菌菌毛抗原的探针引物组、试剂盒及应用
EP4196605A1 (en) 2021-09-01 2023-06-21 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array

Family Cites Families (134)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US337808A (en) 1886-03-16 Cash and parcel transmitting apparatus for store-service
EP0197196A1 (en) 1985-03-08 1986-10-15 The University Of Rochester Electro-electron optical oscilloscope system for time-resolving picosecond electrical waveforms
DE3511117A1 (de) 1985-03-27 1986-10-02 Fa. August Meyer, 4926 Dörentrup Elektrische steck- und/oder schaltvorrichtung
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5449602A (en) 1988-01-13 1995-09-12 Amoco Corporation Template-directed photoligation
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
EP2332958B1 (en) 1996-02-09 2016-04-20 Cornell Research Foundation, Inc. Detection of nucleic and sequence differences using the ligase detection reaction with addressable arrays
CA2255774C (en) 1996-05-29 2008-03-18 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6312892B1 (en) 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
US6770748B2 (en) 1997-03-07 2004-08-03 Takeshi Imanishi Bicyclonucleoside and oligonucleotide analogue
US6794499B2 (en) 1997-09-12 2004-09-21 Exiqon A/S Oligonucleotide analogues
US6027998A (en) 1997-12-17 2000-02-22 Advanced Micro Devices, Inc. Method for fully planarized conductive line for a stack gate
US6506594B1 (en) 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6521428B1 (en) 1999-04-21 2003-02-18 Genome Technologies, Llc Shot-gun sequencing and amplification without cloning
KR100865105B1 (ko) 1999-06-28 2008-10-24 캘리포니아 인스티튜트 오브 테크놀로지 마이크로 가공된 탄성중합체 밸브 및 펌프 시스템
US6899137B2 (en) 1999-06-28 2005-05-31 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US6262490B1 (en) 1999-11-05 2001-07-17 Advanced Semiconductor Engineering, Inc. Substrate strip for use in packaging semiconductor chips
US6618679B2 (en) 2000-01-28 2003-09-09 Althea Technologies, Inc. Methods for analysis of gene expression
US20010031468A1 (en) 2000-02-08 2001-10-18 Alex Chenchik Analyte assays employing universal arrays
US6645432B1 (en) 2000-05-25 2003-11-11 President & Fellows Of Harvard College Microfluidic systems including three-dimensionally arrayed channel networks
EP1313880A2 (en) 2000-05-30 2003-05-28 PE Corporation (NY) Methods for detecting target nucleic acids using coupled ligation and amplification
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
AU8356201A (en) 2000-08-11 2002-02-25 Agilix Corp Ultra-sensitive detection systems
GB0022458D0 (en) 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
WO2002023163A1 (en) 2000-09-15 2002-03-21 California Institute Of Technology Microfabricated crossflow devices and methods
US7258774B2 (en) 2000-10-03 2007-08-21 California Institute Of Technology Microfluidic devices and methods of use
WO2002065005A1 (en) 2000-11-06 2002-08-22 California Institute Of Technology Electrostatic valves for microfluidic devices
WO2002040874A1 (en) 2000-11-16 2002-05-23 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
US20040110191A1 (en) 2001-01-31 2004-06-10 Winkler Matthew M. Comparative analysis of nucleic acids using population tagging
US20020164816A1 (en) 2001-04-06 2002-11-07 California Institute Of Technology Microfluidic sample separation device
US6752922B2 (en) 2001-04-06 2004-06-22 Fluidigm Corporation Microfluidic chromatography
US6960437B2 (en) 2001-04-06 2005-11-01 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
US20030170675A1 (en) 2001-04-11 2003-09-11 The Gov't Of The U.S Of America As Represented By The Secretary Of The Dept. Of Health & Human Serv. Methods of manipulating nucleic acids
US7153656B2 (en) 2001-09-11 2006-12-26 Los Alamos National Security, Llc Nucleic acid sequence detection using multiplexed oligonucleotide PCR
WO2003033741A1 (en) 2001-10-16 2003-04-24 Aclara Biosciences, Inc. Universal e-tag primer and probe compositions and methods
US20030077611A1 (en) 2001-10-24 2003-04-24 Sention Methods and systems for dynamic gene expression profiling
EP1463796B1 (en) 2001-11-30 2013-01-09 Fluidigm Corporation Microfluidic device and methods of using same
US20030119004A1 (en) 2001-12-05 2003-06-26 Wenz H. Michael Methods for quantitating nucleic acids using coupled ligation and amplification
US20060035222A1 (en) 2002-01-15 2006-02-16 Knut Rudi Methods of nucleic acid amplification
WO2003085379A2 (en) 2002-04-01 2003-10-16 Fluidigm Corporation Microfluidic particle-analysis systems
CA2497988C (en) 2002-09-06 2011-03-29 The Trustees Of Boston University Quantification of gene expression
JP2006500034A (ja) 2002-09-19 2006-01-05 アプレラ コーポレイション 標的を検出するための方法および組成物
US8220494B2 (en) 2002-09-25 2012-07-17 California Institute Of Technology Microfluidic large scale integration
US7143785B2 (en) 2002-09-25 2006-12-05 California Institute Of Technology Microfluidic large scale integration
WO2004040001A2 (en) * 2002-10-02 2004-05-13 California Institute Of Technology Microfluidic nucleic acid analysis
US20040086892A1 (en) 2002-11-06 2004-05-06 Crothers Donald M. Universal tag assay
AU2003298706A1 (en) 2002-12-04 2004-06-23 Applera Corporation Multiplex amplification of polynucleotides
US20040110153A1 (en) 2002-12-10 2004-06-10 Affymetrix, Inc. Compleixity management of genomic DNA by semi-specific amplification
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US20040209299A1 (en) 2003-03-07 2004-10-21 Rubicon Genomics, Inc. In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
EP1649055B1 (en) 2003-06-27 2009-05-20 Nanosphere, Inc. Bio-barcode based detection of target analytes
CA2536565A1 (en) 2003-09-10 2005-05-12 Althea Technologies, Inc. Expression profiling using microarrays
KR100571817B1 (ko) 2003-09-19 2006-04-17 삼성전자주식회사 태그 서열에 혼성화하는 검출 프로브를 이용하는 표적핵산의 검출방법
ATE551383T1 (de) 2003-09-23 2012-04-15 Univ North Carolina Photohärtbare perfluorpolyether zur verwendung als neue werkstoffe in mikrofluidischen vorrichtungen
KR100830623B1 (ko) 2003-10-13 2008-05-20 제나코 바이오메디컬 프로덕츠, 인코포레이티드 핵산의 프라이머 기초 멀티플렉스 증폭을 위한 방법 및 키트
US20050095634A1 (en) 2003-10-16 2005-05-05 Genomic Health Inc. qRT-PCR assay system for gene expression profiling
WO2005064020A1 (en) 2003-12-23 2005-07-14 Autogenomics, Inc. Multiplexed nucleic acid analysis with high specificity
CN101189271A (zh) 2004-02-13 2008-05-28 北卡罗来纳大学查珀尔希尔分校 制造微流体设备的功能材料和新型方法
EP1727913B8 (en) 2004-03-24 2009-08-19 Applied Biosystems, LLC Ligation and amplification reactions for determining target molecules
JP5502275B2 (ja) 2004-05-02 2014-05-28 フルイディグム コーポレイション 熱反応デバイスおよびその熱反応デバイスの使用方法
US20060053503A1 (en) 2004-07-30 2006-03-09 Ut-Battelle, Llc Cranial and vertebral defects associated with loss-of-function of Nell
DE602005024987D1 (de) 2004-08-24 2011-01-05 Cornell Res Foundation Inc Detektion von nucleinsäureunterschieden mit hilfe von endonucleasespaltungs-/ligaseabgabereaktionen und kapillarelektrophoresen oder mikroarrays
WO2006071582A2 (en) 2004-12-29 2006-07-06 Applera Corporation Methods, compositions, and kits for forming self-complementary polynucleotides
WO2006128010A2 (en) 2005-05-26 2006-11-30 The Trustees Of Boston University Quantification of nucleic acids and proteins using oligonucleotide mass tags
WO2007117256A1 (en) 2005-05-31 2007-10-18 Applera Corporation Multiplexed amplification of short nucleic acids
US20090317798A1 (en) 2005-06-02 2009-12-24 Heid Christian A Analysis using microfluidic partitioning devices
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
WO2007024800A2 (en) 2005-08-22 2007-03-01 Applera Corporation Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other
WO2007033385A2 (en) * 2005-09-13 2007-03-22 Fluidigm Corporation Microfluidic assay devices and methods
CA2623539C (en) 2005-09-29 2015-12-15 Keygene N.V. High throughput screening of mutagenized populations
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
EP1994179A2 (en) 2006-02-18 2008-11-26 Michael Strathmann Massively multiplexed sequencing
ES2301342B1 (es) 2006-03-14 2009-05-01 Oryzon Genomics, S.A. "metodo de analisis de acidos nucleicos".
US8828661B2 (en) * 2006-04-24 2014-09-09 Fluidigm Corporation Methods for detection and quantification of nucleic acid or protein targets in a sample
WO2007147018A1 (en) 2006-06-14 2007-12-21 Cellpoint Diagnostics, Inc. Analysis of rare cell-enriched samples
WO2007149789A2 (en) 2006-06-22 2007-12-27 Applera Corporation Conversion of target specific amplification to universal sequencing
US9328378B2 (en) 2006-07-31 2016-05-03 Illumina Cambridge Limited Method of library preparation avoiding the formation of adaptor dimers
DK2518162T3 (en) 2006-11-15 2018-06-18 Biospherex Llc Multi-tag sequencing and ecogenomic analysis
US9938641B2 (en) 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
EP2125219B1 (en) 2007-01-19 2016-08-10 Fluidigm Corporation High precision microfluidic devices and methods
WO2008097841A2 (en) 2007-02-02 2008-08-14 California Institute Of Technology Surface chemistry and deposition techniques
EP2121983A2 (en) 2007-02-02 2009-11-25 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
CN101067156A (zh) 2007-05-18 2007-11-07 中国人民解放军第三军医大学第一附属医院 一种基于选择性探针的多重pcr方法及其应用
GB0712882D0 (en) 2007-07-03 2007-08-15 Leicester University Of Nucleic acid amplification
WO2009015296A1 (en) 2007-07-24 2009-01-29 The Regents Of The University Of California Microfabricated dropley generator
US20090053719A1 (en) * 2007-08-03 2009-02-26 The Chinese University Of Hong Kong Analysis of nucleic acids by digital pcr
US9440231B2 (en) 2007-08-14 2016-09-13 Fluidigm Corporation Polymer microfluidic biochip fabrication
CA2697640C (en) 2007-09-21 2016-06-21 Katholieke Universiteit Leuven Tools and methods for genetic tests using next generation sequencing
US8268564B2 (en) 2007-09-26 2012-09-18 President And Fellows Of Harvard College Methods and applications for stitched DNA barcodes
US20090163366A1 (en) * 2007-12-24 2009-06-25 Helicos Biosciences Corporation Two-primer sequencing for high-throughput expression analysis
WO2009102896A2 (en) 2008-02-12 2009-08-20 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
CN102015522B (zh) 2008-02-22 2013-07-03 弗卢迪格姆公司 用于微流体器件的集成载体
US9487822B2 (en) 2008-03-19 2016-11-08 Fluidigm Corporation Method and apparatus for determining copy number variation using digital PCR
AU2009232353B2 (en) 2008-04-05 2015-04-30 Single Cell Technology, Inc. Method of screening single cells for the production of biologically active agents
US9068181B2 (en) 2008-05-23 2015-06-30 The General Hospital Corporation Microfluidic droplet encapsulation
EP4047367A1 (en) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Method for detecting target analytes with droplet libraries
US20100069250A1 (en) 2008-08-16 2010-03-18 The Board Of Trustees Of The Leland Stanford Junior University Digital PCR Calibration for High Throughput Sequencing
EP2326732A4 (en) 2008-08-26 2012-11-14 Fluidigm Corp TEST METHODS FOR INCREASED SURPLUS OF SAMPLES AND / OR TARGETS
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
KR20110106922A (ko) 2009-01-13 2011-09-29 플루이다임 코포레이션 단일 세포 핵산 분석
US8450063B2 (en) 2009-01-28 2013-05-28 Fluidigm Corporation Determination of copy number differences by amplification
US8528589B2 (en) 2009-03-23 2013-09-10 Raindance Technologies, Inc. Manipulation of microfluidic droplets
DK3002337T3 (en) 2009-03-30 2019-02-18 Illumina Inc ANALYSIS OF EXPRESSION OF GENES IN SINGLE CELLS
US8691509B2 (en) 2009-04-02 2014-04-08 Fluidigm Corporation Multi-primer amplification method for barcoding of target nucleic acids
US20100285537A1 (en) 2009-04-02 2010-11-11 Fluidigm Corporation Selective tagging of short nucleic acid fragments and selective protection of target sequences from degradation
US20130137094A1 (en) 2010-01-25 2013-05-30 George Mason Intellectual Properties, Inc. One-Step Cell and Tissue Preservative for Morphologic and Molecular Analysis
EP2569447A4 (en) 2010-05-14 2013-11-27 Fluidigm Corp ANALYZES FOR DETECTION OF GENOTYPE, MUTATIONS, AND / OR ANEUPLOIDIE
EP2569453B1 (en) 2010-05-14 2015-12-16 Fluidigm Corporation Nucleic acid isolation methods
GB2497912B (en) 2010-10-08 2014-06-04 Harvard College High-throughput single cell barcoding
EP2668505A4 (en) 2011-01-24 2014-05-21 Epic Sciences Inc METHOD FOR OBTAINING INDIVIDUAL CELLS AND APPLICATIONS FROM INDIVIDUAL YTOMICS
WO2012112804A1 (en) 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
EP2702175B1 (en) 2011-04-25 2018-08-08 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
SG194745A1 (en) 2011-05-20 2013-12-30 Fluidigm Corp Nucleic acid encoding reactions
WO2012166425A2 (en) 2011-05-27 2012-12-06 President And Fellows Of Harvard College Methods of amplifying whole genome of a single cell
US9586987B2 (en) 2011-09-08 2017-03-07 Kabushiki Kaisha Dnaform Primer set for isothermal amplication of a target nucleic acid sequence
CN104364392B (zh) 2012-02-27 2018-05-25 赛卢拉研究公司 用于分子计数的组合物和试剂盒
JP2015519900A (ja) 2012-05-21 2015-07-16 フリューダイム・コーポレイション 粒子集団の単粒子解析方法及び単粒子単離方法
JP6558830B2 (ja) 2012-06-15 2019-08-14 ボード・オブ・リージエンツ，ザ・ユニバーシテイ・オブ・テキサス・システム 複数転写産物のハイスループットシークエンシング
EP2861761A1 (en) 2012-06-15 2015-04-22 Adaptive Biotechnologies Corporation Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
WO2014108850A2 (en) 2013-01-09 2014-07-17 Yeda Research And Development Co. Ltd. High throughput transcriptome analysis
WO2014145128A2 (en) 2013-03-15 2014-09-18 Arnold Lyle J Methods for amplification of nucleic acids utilizing a circularized template prepared from a target nucleic acid
GB2546833B (en) 2013-08-28 2018-04-18 Cellular Res Inc Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels
GB201317301D0 (en) 2013-09-30 2013-11-13 Linnarsson Sten Method for capturing and encoding nucleic acid from a plurality of single cells
US10590483B2 (en) 2014-09-15 2020-03-17 Abvitro Llc High-throughput nucleotide library sequencing
US10993997B2 (en) 2014-12-19 2021-05-04 The Broad Institute, Inc. Methods for profiling the t cell repertoire
EP3262214B1 (en) 2015-02-27 2023-10-25 Standard BioTools Inc. Microfluidic device
WO2017106777A1 (en) 2015-12-16 2017-06-22 Fluidigm Corporation High-level multiplex amplification
CA3027423A1 (en) 2016-07-14 2018-01-18 Fluidigm Corporation Single-cell transcript sequencing

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