DK2414547T3 - Multiprimer-amplifikationsmetode til stregkodning af målnukleinsyrer - Google Patents
Multiprimer-amplifikationsmetode til stregkodning af målnukleinsyrerInfo
- Publication number
- DK2414547T3 DK2414547T3 DK10759511.8T DK10759511T DK2414547T3 DK 2414547 T3 DK2414547 T3 DK 2414547T3 DK 10759511 T DK10759511 T DK 10759511T DK 2414547 T3 DK2414547 T3 DK 2414547T3
- Authority
- DK
- Denmark
- Prior art keywords
- nucleic acids
- target nucleic
- amplification method
- bar coding
- multiprimer
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/087—Multiple sequential chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
- B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US16610509P | 2009-04-02 | 2009-04-02 | |
US16618109P | 2009-04-02 | 2009-04-02 | |
US18632709P | 2009-06-11 | 2009-06-11 | |
US30590710P | 2010-02-18 | 2010-02-18 | |
PCT/US2010/029854 WO2010115154A1 (en) | 2009-04-02 | 2010-04-02 | Multi-primer amplification method for barcoding of target nucleic acids |
Publications (1)
Publication Number | Publication Date |
---|---|
DK2414547T3 true DK2414547T3 (da) | 2014-06-16 |
Family
ID=42828729
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK10759511.8T DK2414547T3 (da) | 2009-04-02 | 2010-04-02 | Multiprimer-amplifikationsmetode til stregkodning af målnukleinsyrer |
Country Status (13)
Country | Link |
---|---|
US (5) | US8691509B2 (da) |
EP (2) | EP2789694A1 (da) |
JP (2) | JP5985390B2 (da) |
KR (2) | KR101829182B1 (da) |
CN (3) | CN103952288A (da) |
AU (2) | AU2010232439C1 (da) |
CA (2) | CA2757560C (da) |
DK (1) | DK2414547T3 (da) |
EA (1) | EA023190B9 (da) |
HK (2) | HK1170543A1 (da) |
IL (1) | IL215462A (da) |
SG (2) | SG174617A1 (da) |
WO (1) | WO2010115154A1 (da) |
Families Citing this family (264)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20100022414A1 (en) | 2008-07-18 | 2010-01-28 | Raindance Technologies, Inc. | Droplet Libraries |
EP1720636A4 (en) | 2004-02-02 | 2012-06-20 | Univ British Columbia | SCODAPHORESIS, METHODS AND APPARATUS FOR DISPLACING AND CONCENTRATING PARTICLES |
US8529744B2 (en) | 2004-02-02 | 2013-09-10 | Boreal Genomics Corp. | Enrichment of nucleic acid targets |
US10337054B2 (en) | 2004-02-02 | 2019-07-02 | Quantum-Si Incorporated | Enrichment of nucleic acid targets |
US7968287B2 (en) | 2004-10-08 | 2011-06-28 | Medical Research Council Harvard University | In vitro evolution in microfluidic systems |
WO2007044091A2 (en) | 2005-06-02 | 2007-04-19 | Fluidigm Corporation | Analysis using microfluidic partitioning devices |
US11111544B2 (en) | 2005-07-29 | 2021-09-07 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
US9424392B2 (en) | 2005-11-26 | 2016-08-23 | Natera, Inc. | System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals |
US11111543B2 (en) | 2005-07-29 | 2021-09-07 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
US8781532B2 (en) * | 2005-09-19 | 2014-07-15 | Google Inc. | Customized data retrieval applications for mobile devices providing interpretation of markup language data |
JP2010506136A (ja) | 2006-05-11 | 2010-02-25 | レインダンス テクノロジーズ, インコーポレイテッド | 微小流体デバイス |
US8053191B2 (en) | 2006-08-31 | 2011-11-08 | Westend Asset Clearinghouse Company, Llc | Iterative nucleic acid assembly using activation of vector-encoded traits |
WO2008097559A2 (en) | 2007-02-06 | 2008-08-14 | Brandeis University | Manipulation of fluids and reactions in microfluidic systems |
US8592221B2 (en) | 2007-04-19 | 2013-11-26 | Brandeis University | Manipulation of fluids, fluid components and reactions in microfluidic systems |
US8475641B2 (en) | 2008-02-01 | 2013-07-02 | The University Of British Columbia | Methods and apparatus for particle introduction and recovery |
CA2734868C (en) * | 2008-08-26 | 2019-09-10 | Fluidigm Corporation | Assay methods for increased throughput of samples and/or targets |
US20110218123A1 (en) | 2008-09-19 | 2011-09-08 | President And Fellows Of Harvard College | Creation of libraries of droplets and related species |
EP2379753B1 (en) | 2009-01-13 | 2016-12-28 | Fluidigm Corporation | Single-cell nucleic acid analysis |
WO2010088288A2 (en) * | 2009-01-28 | 2010-08-05 | Fluidigm Corporation | Determination of copy number differences by amplification |
EP2789694A1 (en) | 2009-04-02 | 2014-10-15 | Fluidigm Corporation | Microfluidic device with reaction product recovery system |
WO2010126614A2 (en) | 2009-04-30 | 2010-11-04 | Good Start Genetics, Inc. | Methods and compositions for evaluating genetic markers |
WO2011053987A1 (en) * | 2009-11-02 | 2011-05-05 | Nugen Technologies, Inc. | Compositions and methods for targeted nucleic acid sequence selection and amplification |
US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
EP3392349A1 (en) | 2010-02-12 | 2018-10-24 | Raindance Technologies, Inc. | Digital analyte analysis |
US9399797B2 (en) | 2010-02-12 | 2016-07-26 | Raindance Technologies, Inc. | Digital analyte analysis |
US10351905B2 (en) * | 2010-02-12 | 2019-07-16 | Bio-Rad Laboratories, Inc. | Digital analyte analysis |
US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
US11322224B2 (en) | 2010-05-18 | 2022-05-03 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
US11332793B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for simultaneous amplification of target loci |
US9677118B2 (en) | 2014-04-21 | 2017-06-13 | Natera, Inc. | Methods for simultaneous amplification of target loci |
EP2854057B1 (en) | 2010-05-18 | 2018-03-07 | Natera, Inc. | Methods for non-invasive pre-natal ploidy calling |
US11939634B2 (en) | 2010-05-18 | 2024-03-26 | Natera, Inc. | Methods for simultaneous amplification of target loci |
US11339429B2 (en) | 2010-05-18 | 2022-05-24 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
US11332785B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
US10316362B2 (en) | 2010-05-18 | 2019-06-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
US11408031B2 (en) | 2010-05-18 | 2022-08-09 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
US20190010543A1 (en) | 2010-05-18 | 2019-01-10 | Natera, Inc. | Methods for simultaneous amplification of target loci |
US11326208B2 (en) | 2010-05-18 | 2022-05-10 | Natera, Inc. | Methods for nested PCR amplification of cell-free DNA |
CN101921874B (zh) * | 2010-06-30 | 2013-09-11 | 深圳华大基因科技有限公司 | 基于Solexa测序法的检测人类乳头瘤病毒的方法 |
EP3447155A1 (en) | 2010-09-30 | 2019-02-27 | Raindance Technologies, Inc. | Sandwich assays in droplets |
US9187783B2 (en) | 2010-10-04 | 2015-11-17 | Genapsys, Inc. | Systems and methods for automated reusable parallel biological reactions |
EP2625320B1 (en) | 2010-10-08 | 2019-03-27 | President and Fellows of Harvard College | High-throughput single cell barcoding |
EP3705573A1 (en) | 2010-11-12 | 2020-09-09 | Gen9, Inc. | Methods and devices for nucleic acids synthesis |
WO2012064975A1 (en) | 2010-11-12 | 2012-05-18 | Gen9, Inc. | Protein arrays and methods of using and making the same |
US9163281B2 (en) * | 2010-12-23 | 2015-10-20 | Good Start Genetics, Inc. | Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction |
US9738930B2 (en) | 2011-01-28 | 2017-08-22 | The Broad Institute, Inc. | Paired end bead amplification and high throughput sequencing |
US9952237B2 (en) * | 2011-01-28 | 2018-04-24 | Quanterix Corporation | Systems, devices, and methods for ultra-sensitive detection of molecules or particles |
EP3412778A1 (en) | 2011-02-11 | 2018-12-12 | Raindance Technologies, Inc. | Methods for forming mixed droplets |
WO2012112804A1 (en) | 2011-02-18 | 2012-08-23 | Raindance Technoligies, Inc. | Compositions and methods for molecular labeling |
GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
US9476095B2 (en) * | 2011-04-15 | 2016-10-25 | The Johns Hopkins University | Safe sequencing system |
SI3415619T1 (sl) | 2011-04-28 | 2021-04-30 | The Board of Trustees of the Leland Stanford Junior University Office of the General Counsel Building 170, Third Floor, Main Quad | Identifikacija polinukleotidov, povezanih z vzorcem |
TWI465572B (zh) * | 2011-05-19 | 2014-12-21 | Univ Chang Gung | Method, composition and system of amplification and detection of target microbial DNA |
CN112592960A (zh) * | 2011-05-20 | 2021-04-02 | 富鲁达公司 | 核酸编码反应 |
US9926596B2 (en) | 2011-05-27 | 2018-03-27 | Genapsys, Inc. | Systems and methods for genetic and biological analysis |
EP3709018A1 (en) | 2011-06-02 | 2020-09-16 | Bio-Rad Laboratories, Inc. | Microfluidic apparatus for identifying components of a chemical reaction |
US9752176B2 (en) | 2011-06-15 | 2017-09-05 | Ginkgo Bioworks, Inc. | Methods for preparative in vitro cloning |
WO2013010134A2 (en) * | 2011-07-14 | 2013-01-17 | Celula, Inc. | Systems, apparatus and methods for biochemical analysis |
US8658430B2 (en) | 2011-07-20 | 2014-02-25 | Raindance Technologies, Inc. | Manipulating droplet size |
US10364464B2 (en) | 2011-08-08 | 2019-07-30 | The Broad Institute, Inc. | Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence |
EP2559774A1 (en) | 2011-08-17 | 2013-02-20 | Roche Diagniostics GmbH | Improved method for amplification of target nucleic acids using a multi-primer approach |
ES2737957T3 (es) | 2011-08-26 | 2020-01-17 | Gen9 Inc | Composiciones y métodos para el ensamblaje de alta fidelidad de ácidos nucleicos |
US9206418B2 (en) | 2011-10-19 | 2015-12-08 | Nugen Technologies, Inc. | Compositions and methods for directional nucleic acid amplification and sequencing |
EP2785868B1 (en) | 2011-12-01 | 2017-04-12 | Genapsys Inc. | Systems and methods for high efficiency electronic sequencing and detection |
WO2013104994A2 (en) | 2012-01-13 | 2013-07-18 | The University Of British Columbia | Multiple arm apparatus and methods for separation of particles |
SG11201404243WA (en) | 2012-01-26 | 2014-08-28 | Nugen Technologies Inc | Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation |
EP3854873A1 (en) | 2012-02-17 | 2021-07-28 | Fred Hutchinson Cancer Research Center | Compositions and methods for accurately identifying mutations |
US9176031B2 (en) * | 2012-02-24 | 2015-11-03 | Raindance Technologies, Inc. | Labeling and sample preparation for sequencing |
AU2013226081B2 (en) | 2012-02-27 | 2018-06-14 | Becton, Dickinson And Company | Compositions and kits for molecular counting |
WO2013134261A1 (en) | 2012-03-05 | 2013-09-12 | President And Fellows Of Harvard College | Systems and methods for epigenetic sequencing |
US9150853B2 (en) | 2012-03-21 | 2015-10-06 | Gen9, Inc. | Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis |
US8209130B1 (en) | 2012-04-04 | 2012-06-26 | Good Start Genetics, Inc. | Sequence assembly |
AU2013251701A1 (en) * | 2012-04-24 | 2014-10-30 | Gen9, Inc. | Methods for sorting nucleic acids and multiplexed preparative in vitro cloning |
EP2844773B1 (en) | 2012-05-04 | 2017-08-16 | Boreal Genomics Corp. | Biomarker analysis using scodaphoresis |
US20130309667A1 (en) * | 2012-05-15 | 2013-11-21 | Anthony P. Shuber | Primers for analyzing methylated sequences and methods of use thereof |
EP3514243B1 (en) | 2012-05-21 | 2022-08-17 | The Scripps Research Institute | Methods of sample preparation |
WO2013177206A2 (en) | 2012-05-21 | 2013-11-28 | Fluidigm Corporation | Single-particle analysis of particle populations |
EP3346404A1 (en) * | 2012-06-01 | 2018-07-11 | European Molecular Biology Laboratory | High-capacity storage of digital information in dna |
CN104619894B (zh) | 2012-06-18 | 2017-06-06 | 纽亘技术公司 | 用于非期望核酸序列的阴性选择的组合物和方法 |
US20150191719A1 (en) | 2012-06-25 | 2015-07-09 | Gen9, Inc. | Methods for Nucleic Acid Assembly and High Throughput Sequencing |
EP2867376B1 (en) * | 2012-06-28 | 2017-09-06 | Caldera Health Limited | Targeted rna-seq methods and materials for the diagnosis of prostate cancer |
US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US9388465B2 (en) | 2013-02-08 | 2016-07-12 | 10X Genomics, Inc. | Polynucleotide barcode generation |
US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
CN114891871A (zh) | 2012-08-14 | 2022-08-12 | 10X基因组学有限公司 | 微胶囊组合物及方法 |
US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
CN114085896A (zh) * | 2012-09-24 | 2022-02-25 | Cb生物技术公司 | 使用非干扰性噪音消除性多核苷酸鉴定标签的多重焦磷酸测序 |
GB201217888D0 (en) * | 2012-10-05 | 2012-11-21 | Univ Leuven Kath | High-throughput genotyping by sequencing of single cell |
EP2912468B1 (en) | 2012-10-29 | 2018-09-12 | The Johns Hopkins University | Papanicolaou test for ovarian and endometrial cancers |
EP2914745B1 (en) | 2012-11-05 | 2017-09-06 | Rubicon Genomics, Inc. | Barcoding nucleic acids |
CN105531375B (zh) * | 2012-12-10 | 2020-03-03 | 分析生物科学有限公司 | 靶向基因组分析的方法 |
US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
EP3567116A1 (en) * | 2012-12-14 | 2019-11-13 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
WO2014106076A2 (en) * | 2012-12-28 | 2014-07-03 | Quest Diagnostics Investments Incorporated | Universal sanger sequencing from next-gen sequencing amplicons |
WO2014100866A1 (en) | 2012-12-28 | 2014-07-03 | Fleury S.A. | Method for complete tracking of a set of biological samples containing dna or rna through molecular barcode identification during laboratorial workflow and kit for collecting biological samples containing dna or rna |
EP2943590A4 (en) | 2013-01-13 | 2017-02-15 | Unitaq Bio | Methods and compositions for pcr using blocked and universal primers |
WO2014130890A1 (en) * | 2013-02-21 | 2014-08-28 | Toma Biosciences, Inc. | Methods, compositions, and kits for nucleic acid analysis |
US10100361B2 (en) * | 2013-03-14 | 2018-10-16 | Quest Diagnostics Investments Incorporated | Method for detecting cystic fibrosis |
US9822408B2 (en) | 2013-03-15 | 2017-11-21 | Nugen Technologies, Inc. | Sequential sequencing |
CN105051214B (zh) | 2013-03-15 | 2018-12-28 | 吉纳普赛斯股份有限公司 | 用于生物分析的系统和方法 |
US9340835B2 (en) | 2013-03-15 | 2016-05-17 | Boreal Genomics Corp. | Method for separating homoduplexed and heteroduplexed nucleic acids |
DK3013983T3 (da) | 2013-06-25 | 2023-03-06 | Prognosys Biosciences Inc | Spatialt kodede biologiske assays ved brug af en mikrofluidisk anordning |
CA2915499A1 (en) * | 2013-06-27 | 2014-12-31 | 10X Genomics, Inc. | Compositions and methods for sample processing |
KR102402446B1 (ko) | 2013-08-28 | 2022-05-30 | 벡톤 디킨슨 앤드 컴퍼니 | 대량의 동시 단일 세포 분석 |
US10395758B2 (en) | 2013-08-30 | 2019-08-27 | 10X Genomics, Inc. | Sequencing methods |
US11901041B2 (en) | 2013-10-04 | 2024-02-13 | Bio-Rad Laboratories, Inc. | Digital analysis of nucleic acid modification |
US9582877B2 (en) | 2013-10-07 | 2017-02-28 | Cellular Research, Inc. | Methods and systems for digitally counting features on arrays |
WO2015069634A1 (en) | 2013-11-08 | 2015-05-14 | President And Fellows Of Harvard College | Microparticles, methods for their preparation and use |
CN105849264B (zh) | 2013-11-13 | 2019-09-27 | 纽亘技术公司 | 用于鉴别重复测序读数的组合物和方法 |
WO2015089238A1 (en) | 2013-12-11 | 2015-06-18 | Genapsys, Inc. | Systems and methods for biological analysis and computation |
US9944977B2 (en) | 2013-12-12 | 2018-04-17 | Raindance Technologies, Inc. | Distinguishing rare variations in a nucleic acid sequence from a sample |
US9824068B2 (en) | 2013-12-16 | 2017-11-21 | 10X Genomics, Inc. | Methods and apparatus for sorting data |
EP3102722B1 (en) | 2014-02-04 | 2020-08-26 | Jumpcode Genomics, Inc. | Genome fractioning |
CA2940669C (en) * | 2014-02-27 | 2023-10-03 | Igenomx International Genomics Corporation | Methods for analysis of somatic mobile elements, and uses thereof |
WO2015131107A1 (en) | 2014-02-28 | 2015-09-03 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
US9694361B2 (en) | 2014-04-10 | 2017-07-04 | 10X Genomics, Inc. | Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same |
US9822401B2 (en) | 2014-04-18 | 2017-11-21 | Genapsys, Inc. | Methods and systems for nucleic acid amplification |
US20150298091A1 (en) | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
AU2015249846B2 (en) | 2014-04-21 | 2021-07-22 | Natera, Inc. | Detecting mutations and ploidy in chromosomal segments |
LT3456846T (lt) * | 2014-04-21 | 2022-09-12 | President And Fellows Of Harvard College | Nukleorūgšties unikalios sekos įvedimo sistemos ir būdai |
WO2015187953A2 (en) * | 2014-06-04 | 2015-12-10 | Quest Diagnostics Investments Incorporated | Method for direct microbial identification |
CN113249435A (zh) | 2014-06-26 | 2021-08-13 | 10X基因组学有限公司 | 分析来自单个细胞或细胞群体的核酸的方法 |
WO2015200891A1 (en) | 2014-06-26 | 2015-12-30 | 10X Technologies, Inc. | Processes and systems for nucleic acid sequence assembly |
WO2016011280A1 (en) * | 2014-07-16 | 2016-01-21 | Tangen Biosciences, Inc. | Isothermal methods for amplifying nucleic acid samples |
EP3177740B1 (en) | 2014-08-06 | 2021-01-13 | Nugen Technologies, Inc. | Digital measurements from targeted sequencing |
US10450562B2 (en) | 2014-09-09 | 2019-10-22 | Igenomx International Genomics Corporation | Methods and compositions for rapid nucleic acid library preparation |
US11408024B2 (en) | 2014-09-10 | 2022-08-09 | Molecular Loop Biosciences, Inc. | Methods for selectively suppressing non-target sequences |
BR112017008877A2 (pt) | 2014-10-29 | 2018-07-03 | 10X Genomics Inc | métodos e composições para sequenciamento de ácido nucleico-alvo |
WO2016073353A1 (en) | 2014-11-03 | 2016-05-12 | Tangen Biosciences, Inc. | Apparatus and method for cell, spore, or virus capture and disruption |
US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
US20160194701A1 (en) * | 2014-11-21 | 2016-07-07 | Nanostring Technologies, Inc. | Enzyme- and amplification-free sequencing |
US10066259B2 (en) | 2015-01-06 | 2018-09-04 | Good Start Genetics, Inc. | Screening for structural variants |
AU2016207023B2 (en) | 2015-01-12 | 2019-12-05 | 10X Genomics, Inc. | Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same |
CN107209814B (zh) | 2015-01-13 | 2021-10-15 | 10X基因组学有限公司 | 用于使结构变异和相位信息可视化的系统和方法 |
CN107250381B (zh) * | 2015-01-16 | 2022-03-08 | 斯库维尔公司 | Dna集合的归一化迭代条形码和测序 |
WO2016115457A1 (en) * | 2015-01-16 | 2016-07-21 | California Institute Of Technology | Methods and devices for micro-isolation, extraction, and/or analysis of microscale components in an array |
MX2017010142A (es) | 2015-02-09 | 2017-12-11 | 10X Genomics Inc | Sistemas y metodos para determinar variacion estructural y ajuste de fases con datos de recuperacion de variantes. |
WO2016134078A1 (en) | 2015-02-19 | 2016-08-25 | Becton, Dickinson And Company | High-throughput single-cell analysis combining proteomic and genomic information |
WO2016137973A1 (en) | 2015-02-24 | 2016-09-01 | 10X Genomics Inc | Partition processing methods and systems |
CN115651972A (zh) | 2015-02-24 | 2023-01-31 | 10X 基因组学有限公司 | 用于靶向核酸序列覆盖的方法 |
WO2016138292A1 (en) | 2015-02-25 | 2016-09-01 | Igenomx International Genomics Corporation | Methods and compositions for in silico long read sequencing |
EP3262192B1 (en) | 2015-02-27 | 2020-09-16 | Becton, Dickinson and Company | Spatially addressable molecular barcoding |
CA2976681A1 (en) | 2015-02-27 | 2016-09-01 | Fluidigm Corporation | Single-cell nucleic acids for high-throughput studies |
WO2016160844A2 (en) | 2015-03-30 | 2016-10-06 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
JP6828007B2 (ja) | 2015-04-10 | 2021-02-10 | スペーシャル トランスクリプトミクス アクチボラグ | 生物学的試料の空間識別されるマルチプレックスな核酸分析 |
JP2018511341A (ja) | 2015-04-17 | 2018-04-26 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 遺伝子配列決定および他の適用のためのバーコード化システムおよび方法 |
US10385387B2 (en) | 2015-04-20 | 2019-08-20 | Pacific Biosciences Of California, Inc. | Methods for selectively amplifying and tagging nucleic acids |
EP3286326A1 (en) | 2015-04-23 | 2018-02-28 | Cellular Research, Inc. | Methods and compositions for whole transcriptome amplification |
US11479812B2 (en) | 2015-05-11 | 2022-10-25 | Natera, Inc. | Methods and compositions for determining ploidy |
WO2016185284A1 (en) | 2015-05-20 | 2016-11-24 | Boreal Genomics, Inc. | Method for isolating target nucleic acid using heteroduplex binding proteins |
US11124823B2 (en) | 2015-06-01 | 2021-09-21 | Becton, Dickinson And Company | Methods for RNA quantification |
KR102625365B1 (ko) * | 2015-07-07 | 2024-01-15 | 필러 바이오사이언시스 인코포레이티드 | 프라이머-다이머 증폭 감소 방법 |
WO2017027653A1 (en) | 2015-08-11 | 2017-02-16 | The Johns Hopkins University | Assaying ovarian cyst fluid |
EP3347465B1 (en) | 2015-09-11 | 2019-06-26 | Cellular Research, Inc. | Methods and compositions for nucleic acid library normalization |
JP2018537414A (ja) | 2015-10-13 | 2018-12-20 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ゲルマイクロスフェアの作製及び使用のためのシステム及び方法 |
CA3004310A1 (en) * | 2015-11-04 | 2017-05-11 | Atreca, Inc. | Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells |
BR112018009606A8 (pt) | 2015-11-11 | 2019-02-26 | Resolution Bioscience Inc | construção de alta eficiência de bibliotecas de dna |
EP3384048B1 (en) | 2015-12-04 | 2021-03-24 | 10X Genomics, Inc. | Methods and compositions for nucleic acid analysis |
CN108603224B (zh) | 2015-12-16 | 2022-06-28 | 富鲁达公司 | 高水平多路复用扩增 |
JP6735348B2 (ja) | 2016-02-11 | 2020-08-05 | 10エックス ジェノミクス, インコーポレイテッド | 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体 |
US11339427B2 (en) | 2016-02-12 | 2022-05-24 | Jumpcode Genomics, Inc. | Method for target specific RNA transcription of DNA sequences |
JP5997407B1 (ja) * | 2016-04-21 | 2016-09-28 | 日鉄住金環境株式会社 | 多項目増幅手法 |
ES2956757T3 (es) | 2016-05-02 | 2023-12-27 | Becton Dickinson Co | Codificación con códigos de barras moleculares precisa |
WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
WO2017205691A1 (en) | 2016-05-26 | 2017-11-30 | Cellular Research, Inc. | Molecular label counting adjustment methods |
US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
WO2018013723A1 (en) * | 2016-07-14 | 2018-01-18 | Fluidigm Corporation | Single-cell transcript sequencing |
CN116397014A (zh) | 2016-07-20 | 2023-07-07 | 测序健康公司 | 用于核酸测序的系统和方法 |
JP7217224B2 (ja) | 2016-08-25 | 2023-02-02 | レゾリューション バイオサイエンス, インコーポレイテッド | Dna試料中のゲノムコピー変化の検出方法 |
CA3034924A1 (en) | 2016-09-26 | 2018-03-29 | Cellular Research, Inc. | Measurement of protein expression using reagents with barcoded oligonucleotide sequences |
US11485996B2 (en) | 2016-10-04 | 2022-11-01 | Natera, Inc. | Methods for characterizing copy number variation using proximity-litigation sequencing |
ES2870639T3 (es) | 2016-10-24 | 2021-10-27 | Geneinfosec Inc | Ocultación de información presente en los ácidos nucleicos |
EP3539035B1 (en) | 2016-11-08 | 2024-04-17 | Becton, Dickinson and Company | Methods for expression profile classification |
EP3538672A1 (en) | 2016-11-08 | 2019-09-18 | Cellular Research, Inc. | Methods for cell label classification |
EP4282981A3 (en) * | 2016-11-17 | 2024-01-17 | Combinati Incorporated | Methods and systems for nucleic acid analysis and quantification |
JP6730525B2 (ja) | 2016-11-21 | 2020-07-29 | ナノストリング テクノロジーズ,インコーポレイティド | 化学組成物とそれを利用する方法 |
US10011870B2 (en) | 2016-12-07 | 2018-07-03 | Natera, Inc. | Compositions and methods for identifying nucleic acid molecules |
US10813383B2 (en) * | 2016-12-12 | 2020-10-27 | R.J. Reynolds Tobacco Company | Dehydration of tobacco and tobacco-derived materials |
CA3046953A1 (en) | 2016-12-12 | 2018-06-21 | Dana Farber Cancer Institute, Inc. | Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection |
US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
EP3568234B1 (en) | 2017-01-13 | 2023-09-06 | Cellular Research, Inc. | Hydrophilic coating of fluidic channels |
CN117512066A (zh) | 2017-01-30 | 2024-02-06 | 10X基因组学有限公司 | 用于基于微滴的单细胞条形编码的方法和系统 |
WO2018144240A1 (en) | 2017-02-01 | 2018-08-09 | Cellular Research, Inc. | Selective amplification using blocking oligonucleotides |
EP3357575B1 (en) * | 2017-02-06 | 2021-03-17 | H. Hoffnabb-La Roche Ag | Sealable microfluidic chip and method for thermocycling |
CN110582580A (zh) * | 2017-03-24 | 2019-12-17 | 约翰斯霍普金斯大学 | 链特异性检测亚硫酸氢盐转化的双链体 |
WO2018183942A1 (en) | 2017-03-31 | 2018-10-04 | Grail, Inc. | Improved library preparation and use thereof for sequencing-based error correction and/or variant identification |
CN106835292B (zh) * | 2017-04-05 | 2019-04-09 | 北京泛生子基因科技有限公司 | 一步法快速构建扩增子文库的方法 |
CN110870018A (zh) | 2017-05-19 | 2020-03-06 | 10X基因组学有限公司 | 用于分析数据集的系统和方法 |
US10400235B2 (en) | 2017-05-26 | 2019-09-03 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
CN109526228B (zh) | 2017-05-26 | 2022-11-25 | 10X基因组学有限公司 | 转座酶可接近性染色质的单细胞分析 |
US10676779B2 (en) | 2017-06-05 | 2020-06-09 | Becton, Dickinson And Company | Sample indexing for single cells |
US11174511B2 (en) * | 2017-07-24 | 2021-11-16 | Dana-Farber Cancer Institute, Inc. | Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture |
WO2019060628A1 (en) | 2017-09-21 | 2019-03-28 | Genapsys, Inc. | SYSTEMS AND METHODS FOR NUCLEIC ACID SEQUENCING |
US11084037B2 (en) * | 2017-09-25 | 2021-08-10 | Plexium, Inc. | Oligonucleotide encoded chemical libraries |
US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
JP2021500901A (ja) * | 2017-10-26 | 2021-01-14 | エッセンリックス コーポレーション | Crofおよび機械学習を使用する画像ベースのアッセイのシステムおよび方法 |
SG11201913654QA (en) | 2017-11-15 | 2020-01-30 | 10X Genomics Inc | Functionalized gel beads |
US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
JP7175326B2 (ja) | 2017-11-29 | 2022-11-18 | パナジーン・インコーポレイテッド | 標的核酸増幅方法及び標的核酸増幅用組成物 |
WO2019126209A1 (en) | 2017-12-19 | 2019-06-27 | Cellular Research, Inc. | Particles associated with oligonucleotides |
CA3044782A1 (en) | 2017-12-29 | 2019-06-29 | Clear Labs, Inc. | Automated priming and library loading device |
EP3775271A1 (en) | 2018-04-06 | 2021-02-17 | 10X Genomics, Inc. | Systems and methods for quality control in single cell processing |
EP3560593A1 (en) * | 2018-04-25 | 2019-10-30 | OPTOLANE Technologies Inc. | Catridge for digital real-time pcr |
ES2945191T3 (es) | 2018-05-03 | 2023-06-29 | Becton Dickinson Co | Análisis de muestras multiómicas de alto rendimiento |
EP3788170A1 (en) | 2018-05-03 | 2021-03-10 | Becton, Dickinson and Company | Molecular barcoding on opposite transcript ends |
US11549139B2 (en) | 2018-05-14 | 2023-01-10 | Nanostring Technologies, Inc. | Chemical compositions and methods of using same |
US11525159B2 (en) | 2018-07-03 | 2022-12-13 | Natera, Inc. | Methods for detection of donor-derived cell-free DNA |
CN108998508B (zh) * | 2018-08-28 | 2022-09-16 | 深圳联合医学科技有限公司 | 扩增子测序文库的构建方法及引物组和试剂盒 |
US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
CN112805389A (zh) | 2018-10-01 | 2021-05-14 | 贝克顿迪金森公司 | 确定5’转录物序列 |
EP3877520A1 (en) | 2018-11-08 | 2021-09-15 | Becton Dickinson and Company | Whole transcriptome analysis of single cells using random priming |
WO2020123311A2 (en) | 2018-12-10 | 2020-06-18 | 10X Genomics, Inc. | Resolving spatial arrays using deconvolution |
US11492660B2 (en) | 2018-12-13 | 2022-11-08 | Becton, Dickinson And Company | Selective extension in single cell whole transcriptome analysis |
EP3666904A1 (en) | 2018-12-14 | 2020-06-17 | Lexogen GmbH | Nucleic acid amplification and identification method |
US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
WO2020150356A1 (en) | 2019-01-16 | 2020-07-23 | Becton, Dickinson And Company | Polymerase chain reaction normalization through primer titration |
EP4242322A3 (en) | 2019-01-23 | 2023-09-20 | Becton, Dickinson and Company | Oligonucleotides associated with antibodies |
JPWO2020184720A1 (da) * | 2019-03-13 | 2020-09-17 | ||
US11939622B2 (en) | 2019-07-22 | 2024-03-26 | Becton, Dickinson And Company | Single cell chromatin immunoprecipitation sequencing assay |
US11773436B2 (en) | 2019-11-08 | 2023-10-03 | Becton, Dickinson And Company | Using random priming to obtain full-length V(D)J information for immune repertoire sequencing |
EP4025711A2 (en) | 2019-11-08 | 2022-07-13 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
EP4073270A4 (en) * | 2019-12-09 | 2024-01-10 | Laboratorios Maymo S A | RAPID AMPLIFICATION AND GENOTYPING OF NUCLEIC ACID SEQUENCES |
CN114885610A (zh) | 2019-12-23 | 2022-08-09 | 10X基因组学有限公司 | 使用rna模板化连接进行空间分析的方法 |
US11649497B2 (en) | 2020-01-13 | 2023-05-16 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and RNA |
US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
EP4103580A4 (en) | 2020-02-13 | 2024-03-06 | Zymergen Inc | METAGENOMIC LIBRARY AND NATURAL PRODUCT DISCOVERY PLATFORM |
US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
CN111394432B (zh) * | 2020-03-27 | 2023-03-24 | 深圳闪量科技有限公司 | 基于通用探针芯片的多重定量pcr检测系统 |
CN115916999A (zh) | 2020-04-22 | 2023-04-04 | 10X基因组学有限公司 | 用于使用靶向rna耗竭进行空间分析的方法 |
CA3176541A1 (en) * | 2020-04-24 | 2021-10-28 | Oliver Gediminas WACHTER | Single step sample preparation for next generation sequencing |
US11661625B2 (en) | 2020-05-14 | 2023-05-30 | Becton, Dickinson And Company | Primers for immune repertoire profiling |
AU2021275906A1 (en) | 2020-05-22 | 2022-12-22 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
EP4153775A1 (en) | 2020-05-22 | 2023-03-29 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
WO2021242834A1 (en) | 2020-05-26 | 2021-12-02 | 10X Genomics, Inc. | Method for resetting an array |
WO2021252499A1 (en) | 2020-06-08 | 2021-12-16 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
WO2021252591A1 (en) * | 2020-06-10 | 2021-12-16 | 10X Genomics, Inc. | Methods for determining a location of an analyte in a biological sample |
CN116034166A (zh) | 2020-06-25 | 2023-04-28 | 10X基因组学有限公司 | Dna甲基化的空间分析 |
US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
KR102526524B1 (ko) | 2020-09-02 | 2023-04-27 | 한국표준과학연구원 | 전장유전체 증폭을 위한 인간 사스-코로나바이러스-2 범용 프라이머 세트 및 이를 이용한 진단 키트 |
EP4211239A1 (en) * | 2020-09-08 | 2023-07-19 | Seqwell, Inc. | Sequencing oligonucleotides and methods of use thereof |
US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
US11739443B2 (en) | 2020-11-20 | 2023-08-29 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
RU2752840C1 (ru) * | 2020-12-17 | 2021-08-09 | Общество с ограниченной ответственностью «ДНК-дисплей» | Способ штрихкодирования ампликонов при подготовке таргетных библиотек ДНК к массовому параллельному секвенированию |
AU2021409136A1 (en) | 2020-12-21 | 2023-06-29 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
WO2022135598A1 (zh) * | 2020-12-25 | 2022-06-30 | 迈德欣国际有限公司 | 用于空间转录组学分析的生物芯片及其制备方法和应用 |
CN113249505A (zh) * | 2021-06-11 | 2021-08-13 | 内蒙古农业大学 | 鉴定大肠杆菌菌毛抗原的探针引物组、试剂盒及应用 |
EP4196605A1 (en) | 2021-09-01 | 2023-06-21 | 10X Genomics, Inc. | Methods, compositions, and kits for blocking a capture probe on a spatial array |
Family Cites Families (134)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US337808A (en) | 1886-03-16 | Cash and parcel transmitting apparatus for store-service | ||
EP0197196A1 (en) | 1985-03-08 | 1986-10-15 | The University Of Rochester | Electro-electron optical oscilloscope system for time-resolving picosecond electrical waveforms |
DE3511117A1 (de) | 1985-03-27 | 1986-10-02 | Fa. August Meyer, 4926 Dörentrup | Elektrische steck- und/oder schaltvorrichtung |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US5449602A (en) | 1988-01-13 | 1995-09-12 | Amoco Corporation | Template-directed photoligation |
US5066584A (en) * | 1988-09-23 | 1991-11-19 | Cetus Corporation | Methods for generating single stranded dna by the polymerase chain reaction |
US5494810A (en) | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
JP4294732B2 (ja) | 1996-02-09 | 2009-07-15 | コーネル・リサーチ・ファンデーション・インコーポレイテッド | アドレス可能アレイでのリガーゼ検出反応を用いた核酸配列の相違の検出 |
JP4468488B2 (ja) | 1996-05-29 | 2010-05-26 | コーネル・リサーチ・ファンデーション・インコーポレイテッド | 組み合せたリガーゼ検出およびポリメラーゼ連鎖反応を用いる核酸配列相違の検出 |
US6312892B1 (en) | 1996-07-19 | 2001-11-06 | Cornell Research Foundation, Inc. | High fidelity detection of nucleic acid differences by ligase detection reaction |
US6770748B2 (en) | 1997-03-07 | 2004-08-03 | Takeshi Imanishi | Bicyclonucleoside and oligonucleotide analogue |
US6794499B2 (en) | 1997-09-12 | 2004-09-21 | Exiqon A/S | Oligonucleotide analogues |
US6027998A (en) | 1997-12-17 | 2000-02-22 | Advanced Micro Devices, Inc. | Method for fully planarized conductive line for a stack gate |
US6506594B1 (en) | 1999-03-19 | 2003-01-14 | Cornell Res Foundation Inc | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
US6521428B1 (en) | 1999-04-21 | 2003-02-18 | Genome Technologies, Llc | Shot-gun sequencing and amplification without cloning |
US6899137B2 (en) | 1999-06-28 | 2005-05-31 | California Institute Of Technology | Microfabricated elastomeric valve and pump systems |
JP2003524738A (ja) | 1999-06-28 | 2003-08-19 | カリフォルニア インスティチュート オブ テクノロジー | 微細製作エラストマーバルブおよびポンプシステム |
US6262490B1 (en) | 1999-11-05 | 2001-07-17 | Advanced Semiconductor Engineering, Inc. | Substrate strip for use in packaging semiconductor chips |
AU2001234608A1 (en) | 2000-01-28 | 2001-08-07 | Genetrace Systems, Inc. | Methods for analysis of gene expression |
US20010031468A1 (en) | 2000-02-08 | 2001-10-18 | Alex Chenchik | Analyte assays employing universal arrays |
US6645432B1 (en) | 2000-05-25 | 2003-11-11 | President & Fellows Of Harvard College | Microfluidic systems including three-dimensionally arrayed channel networks |
JP2004507226A (ja) | 2000-05-30 | 2004-03-11 | ピーイー コーポレイション (エヌワイ) | ライゲーションおよび増幅の組み合わせを用いて標的核酸を検出するための方法 |
US7351376B1 (en) | 2000-06-05 | 2008-04-01 | California Institute Of Technology | Integrated active flux microfluidic devices and methods |
US6605451B1 (en) | 2000-06-06 | 2003-08-12 | Xtrana, Inc. | Methods and devices for multiplexing amplification reactions |
US6824981B2 (en) | 2000-08-11 | 2004-11-30 | Agilix Corporation | Ultra-sensitive detection systems using alterable peptide tags |
GB0022458D0 (en) | 2000-09-13 | 2000-11-01 | Medical Res Council | Directed evolution method |
AU2001290879A1 (en) | 2000-09-15 | 2002-03-26 | California Institute Of Technology | Microfabricated crossflow devices and methods |
EP1322936A2 (en) | 2000-10-03 | 2003-07-02 | California Institute Of Technology | Microfluidic devices and methods of use |
US7232109B2 (en) | 2000-11-06 | 2007-06-19 | California Institute Of Technology | Electrostatic valves for microfluidic devices |
AU2002230524A1 (en) | 2000-11-16 | 2002-05-27 | California Institute Of Technology | Apparatus and methods for conducting assays and high throughput screening |
US20040110191A1 (en) | 2001-01-31 | 2004-06-10 | Winkler Matthew M. | Comparative analysis of nucleic acids using population tagging |
US6752922B2 (en) | 2001-04-06 | 2004-06-22 | Fluidigm Corporation | Microfluidic chromatography |
US6960437B2 (en) | 2001-04-06 | 2005-11-01 | California Institute Of Technology | Nucleic acid amplification utilizing microfluidic devices |
US20020164816A1 (en) | 2001-04-06 | 2002-11-07 | California Institute Of Technology | Microfluidic sample separation device |
US20030170675A1 (en) | 2001-04-11 | 2003-09-11 | The Gov't Of The U.S Of America As Represented By The Secretary Of The Dept. Of Health & Human Serv. | Methods of manipulating nucleic acids |
US7153656B2 (en) | 2001-09-11 | 2006-12-26 | Los Alamos National Security, Llc | Nucleic acid sequence detection using multiplexed oligonucleotide PCR |
WO2003033741A1 (en) | 2001-10-16 | 2003-04-24 | Aclara Biosciences, Inc. | Universal e-tag primer and probe compositions and methods |
US20030077611A1 (en) | 2001-10-24 | 2003-04-24 | Sention | Methods and systems for dynamic gene expression profiling |
AU2002351187A1 (en) | 2001-11-30 | 2003-06-17 | Fluidigm Corporation | Microfluidic device and methods of using same |
US20030119004A1 (en) | 2001-12-05 | 2003-06-26 | Wenz H. Michael | Methods for quantitating nucleic acids using coupled ligation and amplification |
EP1468117A2 (en) | 2002-01-15 | 2004-10-20 | MATFORSK, Norwegian Food Research Institute | Methods of nucleic acid amplification |
CA2480728A1 (en) | 2002-04-01 | 2003-10-16 | Fluidigm Corporation | Microfluidic particle-analysis systems |
CN1703521B (zh) | 2002-09-06 | 2011-11-16 | 波士顿大学信托人 | 基因表达的定量 |
JP2006500034A (ja) | 2002-09-19 | 2006-01-05 | アプレラ コーポレイション | 標的を検出するための方法および組成物 |
EP2213615A3 (en) | 2002-09-25 | 2012-02-29 | California Institute of Technology | Microfluidic Large Scale Integration |
US8220494B2 (en) | 2002-09-25 | 2012-07-17 | California Institute Of Technology | Microfluidic large scale integration |
US8871446B2 (en) * | 2002-10-02 | 2014-10-28 | California Institute Of Technology | Microfluidic nucleic acid analysis |
US20040086892A1 (en) | 2002-11-06 | 2004-05-06 | Crothers Donald M. | Universal tag assay |
EP3000899A1 (en) | 2002-12-04 | 2016-03-30 | Applied Biosystems, LLC | Multiplex amplification of polynucleotides |
US20040110153A1 (en) | 2002-12-10 | 2004-06-10 | Affymetrix, Inc. | Compleixity management of genomic DNA by semi-specific amplification |
US7575865B2 (en) | 2003-01-29 | 2009-08-18 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
US20040209299A1 (en) | 2003-03-07 | 2004-10-21 | Rubicon Genomics, Inc. | In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA |
US20100022414A1 (en) | 2008-07-18 | 2010-01-28 | Raindance Technologies, Inc. | Droplet Libraries |
US7604965B2 (en) | 2003-04-03 | 2009-10-20 | Fluidigm Corporation | Thermal reaction device and method for using the same |
EP1649055B1 (en) | 2003-06-27 | 2009-05-20 | Nanosphere, Inc. | Bio-barcode based detection of target analytes |
ATE461292T1 (de) | 2003-09-10 | 2010-04-15 | Althea Technologies Inc | Erstellung von expressionsprofilen unter verwendung von mikroarrays |
KR100571817B1 (ko) | 2003-09-19 | 2006-04-17 | 삼성전자주식회사 | 태그 서열에 혼성화하는 검출 프로브를 이용하는 표적핵산의 검출방법 |
AU2004276302B2 (en) | 2003-09-23 | 2011-02-03 | California Institute Of Technology | Photocurable perfluoropolyethers for use as novel materials in microfluidic devices |
BRPI0415435A (pt) | 2003-10-13 | 2006-12-05 | Genaco Biomedical Products Inc | método e kit para amplificação baseada em iniciador de ácidos nucleicos |
CA2542656A1 (en) | 2003-10-16 | 2005-05-06 | Genomic Health, Inc. | Qrt-pcr assay system for gene expression profiling |
DE602004030036D1 (de) | 2003-12-23 | 2010-12-23 | Autogenomics Inc | Multiplexanalyse von Nukleinsäuren zur Bestimmung von Genotypen des humanen Papillomavirus |
JP2007527784A (ja) | 2004-02-13 | 2007-10-04 | ザ ユニバーシティ オブ ノース カロライナ アット チャペル ヒル | マイクロ流体デバイスを作製するための官能性材料及び新規方法 |
ATE399884T1 (de) | 2004-03-24 | 2008-07-15 | Applera Corp | Codierungs- und decodierungsreaktionen zur bestimmung von target-polynukleotiden |
WO2005107938A2 (en) | 2004-05-02 | 2005-11-17 | Fluidigm Corporation | Thermal reaction device and method for using the same |
US20060053503A1 (en) | 2004-07-30 | 2006-03-09 | Ut-Battelle, Llc | Cranial and vertebral defects associated with loss-of-function of Nell |
DE602005024987D1 (de) * | 2004-08-24 | 2011-01-05 | Cornell Res Foundation Inc | Detektion von nucleinsäureunterschieden mit hilfe von endonucleasespaltungs-/ligaseabgabereaktionen und kapillarelektrophoresen oder mikroarrays |
EP1831401B1 (en) | 2004-12-29 | 2010-02-10 | Applied Biosystems, LLC | Methods, compositions, and kits for forming self-complementary polynucleotides |
EP1885890A4 (en) | 2005-05-26 | 2010-01-20 | Univ Boston | QUANTIFICATION OF NUCLEIC ACIDS AND PROTEINS VIA OLIGONUCLEOTIDE MASS LABELS |
EP1896617B1 (en) | 2005-05-31 | 2013-01-02 | Life Technologies Corporation | Multiplex amplification of short nucleic acids |
WO2007044091A2 (en) | 2005-06-02 | 2007-04-19 | Fluidigm Corporation | Analysis using microfluidic partitioning devices |
US20070020640A1 (en) * | 2005-07-21 | 2007-01-25 | Mccloskey Megan L | Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis |
EP2660482B1 (en) * | 2005-08-22 | 2019-08-07 | Life Technologies Corporation | Vorrichtung, System und Verfahren unter Verwendung von nichtmischbaren Flüssigkeiten mit unterschiedlichen Volumen |
WO2007033385A2 (en) * | 2005-09-13 | 2007-03-22 | Fluidigm Corporation | Microfluidic assay devices and methods |
CA2910861C (en) | 2005-09-29 | 2018-08-07 | Michael Josephus Theresia Van Eijk | High throughput screening of mutagenized populations |
WO2007087312A2 (en) | 2006-01-23 | 2007-08-02 | Population Genetics Technologies Ltd. | Molecular counting |
US20070224613A1 (en) | 2006-02-18 | 2007-09-27 | Strathmann Michael P | Massively Multiplexed Sequencing |
ES2301342B1 (es) | 2006-03-14 | 2009-05-01 | Oryzon Genomics, S.A. | "metodo de analisis de acidos nucleicos". |
US8828661B2 (en) | 2006-04-24 | 2014-09-09 | Fluidigm Corporation | Methods for detection and quantification of nucleic acid or protein targets in a sample |
WO2008111990A1 (en) | 2006-06-14 | 2008-09-18 | Cellpoint Diagnostics, Inc. | Rare cell analysis using sample splitting and dna tags |
US20080131937A1 (en) | 2006-06-22 | 2008-06-05 | Applera Corporation | Conversion of Target Specific Amplification to Universal Sequencing |
US9328378B2 (en) | 2006-07-31 | 2016-05-03 | Illumina Cambridge Limited | Method of library preparation avoiding the formation of adaptor dimers |
ES2679996T3 (es) | 2006-11-15 | 2018-09-03 | Biospherex Llc | Secuenciación multi-etiqueta y análisis ecogenómico |
US9938641B2 (en) | 2006-12-18 | 2018-04-10 | Fluidigm Corporation | Selection of aptamers based on geometry |
EP2125219B1 (en) | 2007-01-19 | 2016-08-10 | Fluidigm Corporation | High precision microfluidic devices and methods |
EP2121983A2 (en) | 2007-02-02 | 2009-11-25 | Illumina Cambridge Limited | Methods for indexing samples and sequencing multiple nucleotide templates |
EP2108042A4 (en) | 2007-02-02 | 2010-04-14 | California Inst Of Techn | SURFACE CHEMISTRY AND DEPOSITION TECHNIQUES |
CN101067156A (zh) | 2007-05-18 | 2007-11-07 | 中国人民解放军第三军医大学第一附属医院 | 一种基于选择性探针的多重pcr方法及其应用 |
GB0712882D0 (en) | 2007-07-03 | 2007-08-15 | Leicester University Of | Nucleic acid amplification |
WO2009015296A1 (en) | 2007-07-24 | 2009-01-29 | The Regents Of The University Of California | Microfabricated dropley generator |
US20090053719A1 (en) * | 2007-08-03 | 2009-02-26 | The Chinese University Of Hong Kong | Analysis of nucleic acids by digital pcr |
EP2185285A4 (en) | 2007-08-14 | 2015-08-19 | Arcxis Biotechnologies Inc | PREPARATION OF POLYMERMIC FLUIDIC BIOCHIPS |
CA2697640C (en) | 2007-09-21 | 2016-06-21 | Katholieke Universiteit Leuven | Tools and methods for genetic tests using next generation sequencing |
US8268564B2 (en) | 2007-09-26 | 2012-09-18 | President And Fellows Of Harvard College | Methods and applications for stitched DNA barcodes |
US20090163366A1 (en) * | 2007-12-24 | 2009-06-25 | Helicos Biosciences Corporation | Two-primer sequencing for high-throughput expression analysis |
EP2250283A2 (en) | 2008-02-12 | 2010-11-17 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
US20110053806A1 (en) | 2008-02-22 | 2011-03-03 | Fluidigm Corporation | Integrated carrier for microfluidic device |
US9487822B2 (en) | 2008-03-19 | 2016-11-08 | Fluidigm Corporation | Method and apparatus for determining copy number variation using digital PCR |
EP2260112A4 (en) | 2008-04-05 | 2013-10-09 | Single Cell Technology Inc | SCREENING PROCESS FOR INDIVIDUAL CELLS FOR THE MANUFACTURE OF BIOLOGICAL ACTIVE SUBSTANCES |
US9068181B2 (en) | 2008-05-23 | 2015-06-30 | The General Hospital Corporation | Microfluidic droplet encapsulation |
WO2010021936A1 (en) | 2008-08-16 | 2010-02-25 | The Board Of Trustees Of The Leland Stanford Junior University | Digital pcr calibration for high throughput sequencing |
CA2734868C (en) | 2008-08-26 | 2019-09-10 | Fluidigm Corporation | Assay methods for increased throughput of samples and/or targets |
US8748103B2 (en) | 2008-11-07 | 2014-06-10 | Sequenta, Inc. | Monitoring health and disease status using clonotype profiles |
EP2379753B1 (en) | 2009-01-13 | 2016-12-28 | Fluidigm Corporation | Single-cell nucleic acid analysis |
WO2010088288A2 (en) | 2009-01-28 | 2010-08-05 | Fluidigm Corporation | Determination of copy number differences by amplification |
US8528589B2 (en) | 2009-03-23 | 2013-09-10 | Raindance Technologies, Inc. | Manipulation of microfluidic droplets |
DK2414548T3 (da) | 2009-03-30 | 2015-12-21 | Illumina Inc | Genekspressionsanalyse i enkeltceller |
US20100285537A1 (en) | 2009-04-02 | 2010-11-11 | Fluidigm Corporation | Selective tagging of short nucleic acid fragments and selective protection of target sequences from degradation |
EP2789694A1 (en) | 2009-04-02 | 2014-10-15 | Fluidigm Corporation | Microfluidic device with reaction product recovery system |
US20130137094A1 (en) | 2010-01-25 | 2013-05-30 | George Mason Intellectual Properties, Inc. | One-Step Cell and Tissue Preservative for Morphologic and Molecular Analysis |
SG185543A1 (en) | 2010-05-14 | 2012-12-28 | Fluidigm Corp | Assays for the detection of genotype, mutations, and/or aneuploidy |
SG185544A1 (en) | 2010-05-14 | 2012-12-28 | Fluidigm Corp | Nucleic acid isolation methods |
EP2625320B1 (en) | 2010-10-08 | 2019-03-27 | President and Fellows of Harvard College | High-throughput single cell barcoding |
US20140308669A1 (en) | 2011-01-24 | 2014-10-16 | Epic Science, Inc. | Methods for obtaining single cells and applications of single cell omics |
WO2012112804A1 (en) | 2011-02-18 | 2012-08-23 | Raindance Technoligies, Inc. | Compositions and methods for molecular labeling |
WO2012129363A2 (en) | 2011-03-24 | 2012-09-27 | President And Fellows Of Harvard College | Single cell nucleic acid detection and analysis |
AU2012249759A1 (en) | 2011-04-25 | 2013-11-07 | Bio-Rad Laboratories, Inc. | Methods and compositions for nucleic acid analysis |
CN112592960A (zh) | 2011-05-20 | 2021-04-02 | 富鲁达公司 | 核酸编码反应 |
WO2012166425A2 (en) | 2011-05-27 | 2012-12-06 | President And Fellows Of Harvard College | Methods of amplifying whole genome of a single cell |
JP5299981B1 (ja) | 2011-09-08 | 2013-09-25 | 独立行政法人理化学研究所 | プライマーセット及びそれを用いた標的核酸配列の増幅方法並びに変異核酸の検出方法 |
AU2013226081B2 (en) | 2012-02-27 | 2018-06-14 | Becton, Dickinson And Company | Compositions and kits for molecular counting |
WO2013177206A2 (en) | 2012-05-21 | 2013-11-28 | Fluidigm Corporation | Single-particle analysis of particle populations |
WO2013188831A1 (en) | 2012-06-15 | 2013-12-19 | Adaptive Biotechnologies Corporation | Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set |
ES2774165T3 (es) | 2012-06-15 | 2020-07-17 | Univ Texas | Secuenciación de alto rendimiento de múltiples transcritos de una única célula |
WO2014108850A2 (en) | 2013-01-09 | 2014-07-17 | Yeda Research And Development Co. Ltd. | High throughput transcriptome analysis |
WO2014145128A2 (en) | 2013-03-15 | 2014-09-18 | Arnold Lyle J | Methods for amplification of nucleic acids utilizing a circularized template prepared from a target nucleic acid |
KR102402446B1 (ko) | 2013-08-28 | 2022-05-30 | 벡톤 디킨슨 앤드 컴퍼니 | 대량의 동시 단일 세포 분석 |
GB201317301D0 (en) | 2013-09-30 | 2013-11-13 | Linnarsson Sten | Method for capturing and encoding nucleic acid from a plurality of single cells |
MX2017003382A (es) | 2014-09-15 | 2017-11-20 | Abvitro Llc | Secuenciacion de bibliotecas de nucleotidos de alto rendimiento. |
US10993997B2 (en) | 2014-12-19 | 2021-05-04 | The Broad Institute, Inc. | Methods for profiling the t cell repertoire |
CA2976681A1 (en) | 2015-02-27 | 2016-09-01 | Fluidigm Corporation | Single-cell nucleic acids for high-throughput studies |
CN108603224B (zh) | 2015-12-16 | 2022-06-28 | 富鲁达公司 | 高水平多路复用扩增 |
WO2018013723A1 (en) | 2016-07-14 | 2018-01-18 | Fluidigm Corporation | Single-cell transcript sequencing |
-
2010
- 2010-04-02 EP EP20140158911 patent/EP2789694A1/en not_active Ceased
- 2010-04-02 DK DK10759511.8T patent/DK2414547T3/da active
- 2010-04-02 KR KR1020177022769A patent/KR101829182B1/ko active IP Right Grant
- 2010-04-02 SG SG2011071420A patent/SG174617A1/en unknown
- 2010-04-02 EA EA201171206A patent/EA023190B9/ru not_active IP Right Cessation
- 2010-04-02 AU AU2010232439A patent/AU2010232439C1/en active Active
- 2010-04-02 CA CA2757560A patent/CA2757560C/en active Active
- 2010-04-02 KR KR1020117025826A patent/KR101770363B1/ko active IP Right Grant
- 2010-04-02 CN CN201410139163.8A patent/CN103952288A/zh active Pending
- 2010-04-02 JP JP2012503757A patent/JP5985390B2/ja active Active
- 2010-04-02 EP EP10759511.8A patent/EP2414547B1/en active Active
- 2010-04-02 CN CN201410138786.3A patent/CN103952482A/zh active Pending
- 2010-04-02 WO PCT/US2010/029854 patent/WO2010115154A1/en active Application Filing
- 2010-04-02 SG SG10201402770YA patent/SG10201402770YA/en unknown
- 2010-04-02 CN CN201080021508.XA patent/CN102439177B/zh active Active
- 2010-04-02 CA CA3018687A patent/CA3018687C/en active Active
- 2010-04-02 US US12/753,703 patent/US8691509B2/en active Active
-
2011
- 2011-10-02 IL IL215462A patent/IL215462A/en active IP Right Grant
-
2012
- 2012-11-02 HK HK12111058.2A patent/HK1170543A1/xx not_active IP Right Cessation
-
2014
- 2014-02-13 US US14/180,262 patent/US9677119B2/en active Active
-
2015
- 2015-04-14 HK HK15103623.2A patent/HK1203085A1/xx unknown
- 2015-10-13 AU AU2015242980A patent/AU2015242980A1/en not_active Abandoned
-
2016
- 2016-08-03 JP JP2016152729A patent/JP2016189789A/ja active Pending
-
2017
- 2017-05-09 US US15/590,998 patent/US10344318B2/en active Active
-
2019
- 2019-05-16 US US16/414,705 patent/US20200102594A1/en not_active Abandoned
-
2021
- 2021-02-03 US US17/166,992 patent/US11795494B2/en active Active
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DK2414547T3 (da) | Multiprimer-amplifikationsmetode til stregkodning af målnukleinsyrer | |
GB0921264D0 (en) | Method for amplification of target nucleic acid | |
DK2699698T4 (da) | Oscillerende amplifikations-reaktion for nukleinsyrer | |
DK3428290T3 (da) | Sammensætninger og fremgangsmåder til amplifikation af nukleinsyrer | |
DK2276845T3 (da) | Fremgangsmåde til fremstilling af l-aminosyrer | |
DK3290528T3 (da) | Fremgangsmåder og sammensætninger til nukleinsyresekventering | |
DK2513135T3 (da) | Fremgangsmåde til oprensning af polypeptider | |
BR112012010958A2 (pt) | método para intensificação de feixe | |
DK3354657T3 (da) | Fremgangsmåde til fremstilling af planteafledte proteiner | |
DK3730045T3 (da) | Fremgangsmåde til indføring af en analytsensor | |
DK2300497T3 (da) | Fremgangsmåde til oprensning af koagulationsfaktor VIII | |
DK2325648T3 (da) | Fremgangsmåde til detektering af cancer | |
BRPI1008778A2 (pt) | método de amplificação de modelos de dna ricos em gc | |
DK2611923T3 (da) | Nukleinsyrer, sammensætninger og fremgangsmåder til udskæring af targetnukleinsyrer | |
DK3109326T3 (da) | Sammensætning og fremgangsmåde til nukleinsyresyntese og amplifikation under anvendelse af rt | |
EP2382330A4 (en) | TARGETED NUCLEIC ACID AMPLIFICATION BY CROSS-PRIMING | |
HK1162196A1 (en) | Method for quantification of small rna species rna | |
DK2398817T4 (da) | Fremgangsmåde til rensning af lipopeptider | |
DK2358661T3 (da) | Fremgangsmåde til fremstilling af trans-4-amino-cyclohexyl-eddikesyreethylester-hcl | |
DK3214083T3 (da) | Fremgangsmåde til fremstilling af quinolonforbindelser | |
DK2262739T5 (da) | Fremgangsmåde til neutralisering af mikroorganismer | |
DK2554174T3 (da) | Fremgangsmåde til behandling af sygdomme | |
IS8917A (is) | Aðferð til að smíða skó | |
EP2438184A4 (en) | METHOD FOR AMPLIFYING NUCLEIC ACIDS | |
DK2251168T3 (da) | Fremgangsmåde til fremstilling af træplader |