DK2546339T3 - Muteret alkalisk cellulase - Google Patents

Muteret alkalisk cellulase Download PDF

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Publication number
DK2546339T3
DK2546339T3 DK11753494.1T DK11753494T DK2546339T3 DK 2546339 T3 DK2546339 T3 DK 2546339T3 DK 11753494 T DK11753494 T DK 11753494T DK 2546339 T3 DK2546339 T3 DK 2546339T3
Authority
DK
Denmark
Prior art keywords
glu
ala
asn
amino acid
asp
Prior art date
Application number
DK11753494.1T
Other languages
English (en)
Inventor
Ayako Shimazu
Keiji Endo
Mitsuyoshi Okuda
Original Assignee
Kao Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP2010056059A external-priority patent/JP5698460B2/ja
Priority claimed from JP2010056060A external-priority patent/JP5714830B2/ja
Priority claimed from JP2010063212A external-priority patent/JP5666816B2/ja
Application filed by Kao Corp filed Critical Kao Corp
Application granted granted Critical
Publication of DK2546339T3 publication Critical patent/DK2546339T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38645—Preparations containing enzymes, e.g. protease or amylase containing cellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)

Claims (11)

1. Mutant alkalisk cellulase, der er sammensat af (a) aminosyresekvensen af en alkalisk cellulase beskrevet i SEQ ID NO: 2, eller (b) aminosyresekvensen af en alkalisk cellulase med mindst 70% identitet med aminosyresekvensen af SEQ ID NO: 2 i hvilken aminosyregruppen ved positionen svarende til position 58 af SEQ ID NO:2 er substitueret med en arginin- eller glutaminsyregruppe.
2. Mutant alkalisk cellulasen ifølge krav 1, hvor en signalsekvens sammensat af aminosyregrupperne ved positionerne 1 til 30 af aminosyresekvensen beskrevet i SEQ ID NO: 2 er deleteret.
3. Gen der koder for mutant alkalisk cellulasen ifølge et hvilket som helst af kravene 1 or 2.
4. Rekombinant vektor, omfattende genet ifølge krav 3.
5. Transformant, omfattende den rekombinante vektor ifølge krav 4.
6. Transformanten ifølge krav 5, der er en mikroorganisme.
7. Anti-redepositionsmiddel, omfattende mutant alkalisk cellulasen ifølge krav 1 eller 2.
8. Enzymsammensætning, omfattende mutant alkalisk cellulasen ifølge krav 1 eller 2.
9. Enzymsammensætningen ifølge krav 8, yderligere omfattende ét eller flere enzymer valgt fra gruppen bestående af en protease, en cellulase, en β-glucanase, en hemicellulase, en lipase, en peroxidase, en laccase, en a-amylase, en glucoamylase, en cutinase, en pectinase, en reductase, en oxidase, en phenoloxidase, en ligninase, en pullulanase, en pectatlyase, en xyloglucanase, en xylanase, en pectinacetylesterase, en polygalacturonase, en rhamnogalacturo-nase, en pektinlyase, en anden mannanase, en pektinmethylesterase, en cellobio-hydrolase, en transglutaminase, og blandinger deraf.
10. Rengøringsmiddelsammensætning, omfattende mutant alkalisk cellulasen ifølge krav 1 eller 2, anti-redepositionsmidlet ifølge krav 7, eller enzymsammensætningen ifølge krav 8 eller 9.
11. Fremgangsmåde til forøgelse af anti-redepositionevnen af en alkalisk cellulase, omfattende udskiftning indenfor (a) aminosyresekvensen afen alkalisk cellulase beskrevet i SEQ ID NO: 2; eller (b) aminosyresekvensen afen alkalisk cellulase med mindst 70% identitet med aminosyresekvensen af SEQ ID NO:2, aminosyregruppen ved positionen svarende til position 58 af SEQ ID NO: 2 med en arginin- eller glutaminsyregruppe.
DK11753494.1T 2010-03-12 2011-03-11 Muteret alkalisk cellulase DK2546339T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2010056059A JP5698460B2 (ja) 2010-03-12 2010-03-12 変異アルカリセルラーゼ
JP2010056060A JP5714830B2 (ja) 2010-03-12 2010-03-12 変異アルカリセルラーゼ
JP2010063212A JP5666816B2 (ja) 2010-03-18 2010-03-18 変異アルカリセルラーゼ
PCT/JP2011/055831 WO2011111836A1 (ja) 2010-03-12 2011-03-11 変異アルカリセルラーゼ

Publications (1)

Publication Number Publication Date
DK2546339T3 true DK2546339T3 (da) 2016-10-10

Family

ID=44563633

Family Applications (1)

Application Number Title Priority Date Filing Date
DK11753494.1T DK2546339T3 (da) 2010-03-12 2011-03-11 Muteret alkalisk cellulase

Country Status (5)

Country Link
US (1) US9169458B2 (da)
EP (1) EP2546339B1 (da)
CN (1) CN102782127B (da)
DK (1) DK2546339T3 (da)
WO (1) WO2011111836A1 (da)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5977029B2 (ja) * 2012-01-11 2016-08-24 花王株式会社 変異グリコシドハイドロラーゼ
JP6144483B2 (ja) * 2012-12-05 2017-06-07 花王株式会社 変異グリコシドハイドロラーゼ
JP6081172B2 (ja) * 2012-12-05 2017-02-15 花王株式会社 変異グリコシドハイドロラーゼ
CN104789543B (zh) * 2015-04-25 2017-11-07 上海康地恩生物科技有限公司 一种护色纤维素酶及其突变体
CN105567723B (zh) * 2016-01-21 2018-03-06 河南仰韶生化工程有限公司 一种提高普鲁兰酶活性的方法
US11053488B2 (en) * 2016-12-15 2021-07-06 Danisco Us Inc Polypeptides with endoglucanase activity and uses thereof
JP2022550112A (ja) * 2019-09-29 2022-11-30 ノボザイムス アクティーゼルスカブ 洗剤組成物におけるデオキシリボヌクレアーゼの使用
JP2021069321A (ja) * 2019-10-30 2021-05-06 花王株式会社 変異セルラーゼ
CN113684198B (zh) * 2021-10-27 2022-02-25 中国农业科学院北京畜牧兽医研究所 一种提高纤维素酶催化效率的方法及突变体5i77-m2
CN114591857B (zh) * 2022-03-10 2023-05-09 内蒙古工业大学 一株高纤维降解率的枯草芽孢杆菌及其应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3512981B2 (ja) * 1997-05-19 2004-03-31 花王株式会社 耐熱性アルカリセルラ−ゼ、それを生産する微生物及びその製造方法
WO1999018218A1 (fr) 1997-10-07 1999-04-15 Kao Corporation Protease alcaline
JP2000210081A (ja) 1999-01-21 2000-08-02 Kao Corp 耐熱性アルカリセルラ―ゼ遺伝子
JP4380874B2 (ja) 2000-02-24 2009-12-09 花王株式会社 アルカリセルラーゼ遺伝子
JP2002265998A (ja) 2001-03-15 2002-09-18 Kao Corp 洗浄剤組成物
CN101864406B (zh) 2001-06-06 2016-03-30 诺维信公司 内切-β-1,4-葡聚糖酶
US7041488B2 (en) 2001-06-06 2006-05-09 Novozymes A/S Endo-beta-1,4-glucanase from bacillus
JP4897186B2 (ja) 2002-03-27 2012-03-14 花王株式会社 変異アルカリセルラーゼ
JP4261817B2 (ja) * 2002-04-18 2009-04-30 花王株式会社 液体洗浄剤組成物
JP4417608B2 (ja) * 2002-04-25 2010-02-17 花王株式会社 変異アルカリセルラーゼ
CN1738899A (zh) 2002-12-11 2006-02-22 诺和酶股份有限公司 洗涤剂组合物
JP2006296268A (ja) 2005-04-19 2006-11-02 Kao Corp 組換え微生物

Also Published As

Publication number Publication date
EP2546339B1 (en) 2016-09-07
US20130029897A1 (en) 2013-01-31
EP2546339A4 (en) 2013-08-07
EP2546339A1 (en) 2013-01-16
CN102782127A (zh) 2012-11-14
CN102782127B (zh) 2015-01-28
WO2011111836A1 (ja) 2011-09-15
EP2546339A9 (en) 2013-07-03
US9169458B2 (en) 2015-10-27

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