DK175917B1 - Subtilisinvarianter med foröget termostabilitet - Google Patents

Subtilisinvarianter med foröget termostabilitet Download PDF

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Publication number
DK175917B1
DK175917B1 DK198904964A DK496489A DK175917B1 DK 175917 B1 DK175917 B1 DK 175917B1 DK 198904964 A DK198904964 A DK 198904964A DK 496489 A DK496489 A DK 496489A DK 175917 B1 DK175917 B1 DK 175917B1
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DK
Denmark
Prior art keywords
subtilisin
protein
amino acid
site
metal ion
Prior art date
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DK198904964A
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Danish (da)
English (en)
Other versions
DK496489A (da
DK496489D0 (da
Inventor
Michael W Pantoliano
Barry C Finzel
Philip N Bryan
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Novozymes As
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=21879773&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK175917(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novozymes As filed Critical Novozymes As
Publication of DK496489A publication Critical patent/DK496489A/da
Publication of DK496489D0 publication Critical patent/DK496489D0/da
Application granted granted Critical
Publication of DK175917B1 publication Critical patent/DK175917B1/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Manufacture Of Macromolecular Shaped Articles (AREA)
  • Laminated Bodies (AREA)
  • Adhesives Or Adhesive Processes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
DK198904964A 1987-04-06 1989-10-06 Subtilisinvarianter med foröget termostabilitet DK175917B1 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US3496587A 1987-04-06 1987-04-06
US3496587 1987-04-06
PCT/US1988/001235 WO1988008028A1 (fr) 1987-04-06 1988-04-06 Stabilisation de proteines par mise au point des interactions electrostatiques aux sites de liaison d'ions metalliques
US8801235 1988-04-06

Publications (3)

Publication Number Publication Date
DK496489A DK496489A (da) 1989-10-06
DK496489D0 DK496489D0 (da) 1989-10-06
DK175917B1 true DK175917B1 (da) 2005-06-27

Family

ID=21879773

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198904964A DK175917B1 (da) 1987-04-06 1989-10-06 Subtilisinvarianter med foröget termostabilitet

Country Status (6)

Country Link
EP (2) EP0353250B1 (fr)
JP (2) JP3155984B2 (fr)
AT (2) ATE371029T1 (fr)
DE (3) DE3856362T2 (fr)
DK (1) DK175917B1 (fr)
WO (1) WO1988008028A1 (fr)

Families Citing this family (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
US5324653A (en) * 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
CN1056187C (zh) * 1988-02-11 2000-09-06 金克克国际有限公司 新的蛋白水解酶及其在洗涤剂中的应用
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
AU618675B2 (en) * 1989-05-17 1992-01-02 Amgen, Inc. Multiply mutated subtilisins
JPH04500385A (ja) * 1989-06-26 1992-01-23 ユニリーバー・ナームローゼ・ベンノートシヤープ 酵素洗剤組成物
JPH04500384A (ja) * 1989-06-26 1992-01-23 ユニリーバー・ナームローゼ・ベンノートシヤープ 酵素洗剤組成物
US5665587A (en) 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
US5115102A (en) * 1989-07-21 1992-05-19 Monsanto Company Variant proteins and polypeptides possessing enhanced affinity for immobilized-metal affinity matrices
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
DE69133491T2 (de) * 1990-01-04 2006-07-27 Genencor International, Inc., Palo Alto Glucose-Isomerase mit geändertem pH-Optimum
FI102543B (fi) * 1990-01-04 1998-12-31 Plant Genetic Systems Nv Mutantti glukoosi-isomeraasit
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
MA23346A1 (fr) * 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
AU8079794A (en) * 1993-10-14 1995-05-04 Procter & Gamble Company, The Protease-containing cleaning compositions
US5677272A (en) * 1993-10-14 1997-10-14 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US5922082A (en) 1994-06-16 1999-07-13 Procter & Gamble Company Detergent composition containing wool compatible high alkaline proteases
EP0687733A1 (fr) * 1994-06-16 1995-12-20 The Procter & Gamble Company Composition détergente contenant des protéases très alkalines compatible avec de la laine
US6066611A (en) * 1994-10-13 2000-05-23 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6682924B1 (en) * 1995-05-05 2004-01-27 Novozymes A/S Protease variants and compositions
EP0932667B1 (fr) 1996-11-04 2008-10-01 Novozymes A/S Variants de subtilase et compositions
EP2278001B1 (fr) 1996-11-04 2013-10-23 Novozymes A/S Variants et compositions de protéase
CA2301767A1 (fr) 1997-08-29 1999-03-11 Novo Nordisk A/S Variants de la protease et compositions
JP5095884B2 (ja) 1997-08-29 2012-12-12 ノボザイムス アクティーゼルスカブ プロテアーゼ変異体及び組成物
EP1032655B1 (fr) 1997-11-21 2005-06-29 Novozymes A/S Variantes du type protease et compositions
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
US6780629B2 (en) 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
KR20010034697A (ko) 1998-03-26 2001-04-25 데이비드 엠 모이어 아미노산 치환을 갖는 세린 프로테아제 변이체
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
KR20010052223A (ko) * 1998-03-26 2001-06-25 데이비드 엠 모이어 아미노산 결실 및 치환을 갖는 세린 프로테아제 변이체
EP1183341B2 (fr) 1999-05-20 2012-05-02 Novozymes A/S Enzymes subtilases des sous groupes i-s1 et i-s2 possedant au moins un residu acide amine supplementaire entre les positions 127 et 128
WO2000071691A1 (fr) 1999-05-20 2000-11-30 Novozymes A/S Enzymes de subtilase des sous-groupes i-s1 et i-s2 possedant au moins un residu supplementaire d'acide amine entre les positions 125 et 126
DE60040149D1 (de) 1999-05-20 2008-10-16 Novozymes As Subtilase-enzyme der i-s1 und i-s2 untergruppen mit wenigstens einem zusätzlichen aminosäurerest zwischen positionen 126 und 127
EP1183342B2 (fr) 1999-05-20 2012-06-13 Novozymes A/S Enzymes subtilases des -sous groupes i-s1 et i-s2 possedant au moins un residu acide amine supplementaire entre les positions 128 et 129
DE60040284D1 (de) 1999-05-20 2008-10-30 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 129 und 130
AU4392500A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 132 and 133
CN1399677A (zh) 1999-07-22 2003-02-26 宝洁公司 在确定表位区有氨基酸取代的枯草杆菌蛋白酶变体
CN1373802A (zh) 1999-07-22 2002-10-09 宝洁公司 在特定表位区域具有氨基酸缺失和取代的枯草杆菌蛋白酶变体
KR20020021395A (ko) 1999-07-22 2002-03-20 데이비드 엠 모이어 입체적으로 보호된 ｃｌｉｐ 부위를 갖는 프로테아제콘쥬게이트
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
EP1644471A2 (fr) * 1999-10-14 2006-04-12 Novozymes A/S Compositions detergentes a performance de lavage amelioree et comprenant une variante de subtilase et un tensioactif
US6727085B2 (en) 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
GB0013643D0 (en) 2000-05-31 2000-07-26 Unilever Plc Targeted moieties for use in bleach catalysts
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
BRPI0409992A (pt) 2003-05-07 2006-05-09 Novozymes As enzima subtilase, polinucleotìdeo isolado, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, método para produzir a subtilase, composição limpadora ou detergente, usos de uma subtilase e de uma composição limpadora ou detergente, e, métodos para limpar ou lavar louças, lavar uma superfìcie dura ou lavar roupas e para remover manchas de ovo de uma superfìcie dura ou de roupas para lavar
JP4880469B2 (ja) 2003-10-23 2012-02-22 ノボザイムス アクティーゼルスカブ 洗剤中で改良された安定性を有するプロテアーゼ
DE10360805A1 (de) 2003-12-23 2005-07-28 Henkel Kgaa Neue Alkalische Protease und Wasch- und Reinigungsmittel, enthaltend diese neue Alkalische Protease
CN102250861A (zh) * 2004-02-13 2011-11-23 诺维信公司 蛋白酶变体
DE102004019751A1 (de) 2004-04-23 2005-11-17 Henkel Kgaa Neue Alkalische Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Alkalischen Proteasen
EP1598367A1 (fr) * 2004-05-18 2005-11-23 ZLB Behring GmbH Facteurs de coagulation modifiés de stabilité ameliorée et leur dérivés
WO2007136469A2 (fr) 2006-04-14 2007-11-29 Genencor International, Inc. Traitement en une étape de textiles
CN101460618A (zh) 2006-04-20 2009-06-17 诺维信公司 对蛋渍具有改进的洗涤性能的洒维奈斯变体
MX2009011566A (es) 2007-04-30 2009-11-10 Danisco Us Inc Genencor Div Uso de hidrolizados de proteinas para estabilizar formulaciones detergentes que contienen metaloproteasa.
SG148934A1 (en) 2007-06-11 2009-01-29 Novozymes As A process for combined biopolishing and bleach clean-up
DE102007032111B4 (de) 2007-07-09 2017-07-20 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
DE102007036756A1 (de) 2007-08-03 2009-02-05 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Proteasen
WO2012022777A1 (fr) 2010-08-19 2012-02-23 Novozymes A/S Procédé de criblage d'une sporulation induite
CN112143570A (zh) 2011-07-01 2020-12-29 诺维信公司 液体洗涤剂组合物
EP2970830B1 (fr) 2013-03-14 2017-12-13 Novozymes A/S Films solubles dans l'eau contenant une protéase et un inhibiteur
EP2992076B1 (fr) 2013-05-03 2018-10-24 Novozymes A/S Microencapsulation d'enzymes détergentes
WO2017117089A1 (fr) 2015-12-28 2017-07-06 Novozymes Bioag A/S Prégermination par traitement thermique de spores bactériennes
CN111356762B (zh) 2017-09-27 2024-09-17 诺维信公司 脂肪酶变体和包含此类脂肪酶变体的微囊组合物
EP3749758A1 (fr) 2018-02-08 2020-12-16 Novozymes A/S Variants de lipase et compositions associées
CN111868239A (zh) 2018-02-08 2020-10-30 诺维信公司 脂肪酶、脂肪酶变体及其组合物
CN111770788B (zh) 2018-03-13 2023-07-25 诺维信公司 使用氨基糖低聚物进行微囊化
WO2020099491A1 (fr) 2018-11-14 2020-05-22 Novozymes A/S Composition de soin buccal comprenant un polypeptide ayant une activité dnase
EP3994255A1 (fr) 2019-07-02 2022-05-11 Novozymes A/S Variants de lipase et compositions de ceux-ci
JP7425459B2 (ja) * 2019-10-11 2024-01-31 株式会社シノテスト 安定化されたｈｍｇｂ１含有溶液
CN116322625A (zh) 2020-08-24 2023-06-23 诺维信公司 包含果聚糖酶的口腔护理组合物

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ208612A (en) * 1983-06-24 1991-09-25 Genentech Inc Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations
EP0260299A4 (fr) * 1986-02-12 1988-11-24 Genex Corp Procede de mutagenese et de criblage et produit obtenu.
US5260207A (en) * 1987-04-06 1993-11-09 Enzon Labs Inc. Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
AU618675B2 (en) * 1989-05-17 1992-01-02 Amgen, Inc. Multiply mutated subtilisins

Also Published As

Publication number Publication date
DE3856362D1 (de) 1999-10-14
EP0916732A1 (fr) 1999-05-19
DE353250T1 (de) 1998-05-28
EP0353250A1 (fr) 1990-02-07
ATE371029T1 (de) 2007-09-15
DK496489A (da) 1989-10-06
ATE184316T1 (de) 1999-09-15
EP0353250A4 (en) 1990-09-05
DE3856362T2 (de) 2000-05-18
JP2001029076A (ja) 2001-02-06
JPH02502874A (ja) 1990-09-13
DK496489D0 (da) 1989-10-06
JP3155984B2 (ja) 2001-04-16
WO1988008028A1 (fr) 1988-10-20
EP0353250B1 (fr) 1999-09-08
EP0916732B1 (fr) 2007-08-22
DE3856593D1 (de) 2007-10-04

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