DK175813B1 - Mutantprotease til anvendelse i vaskemidler, DNA-sekvens, som koder for denne, ekspressionsvektor med en sådan sekvens - Google Patents

Mutantprotease til anvendelse i vaskemidler, DNA-sekvens, som koder for denne, ekspressionsvektor med en sådan sekvens Download PDF

Info

Publication number
DK175813B1
DK175813B1 DK198905012A DK501289A DK175813B1 DK 175813 B1 DK175813 B1 DK 175813B1 DK 198905012 A DK198905012 A DK 198905012A DK 501289 A DK501289 A DK 501289A DK 175813 B1 DK175813 B1 DK 175813B1
Authority
DK
Denmark
Prior art keywords
protease
mutant
proteases
detergent
host strain
Prior art date
Application number
DK198905012A
Other languages
Danish (da)
English (en)
Other versions
DK501289D0 (da
DK501289A (da
Inventor
Onno Misset
Leonardus J S M Mulleners
Christiaan A G Van Eekelen
Johannes C Van Der Laan
Roelck A Cuperus
Johan H A Lensink
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8199752&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK175813(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genencor Int filed Critical Genencor Int
Publication of DK501289D0 publication Critical patent/DK501289D0/da
Publication of DK501289A publication Critical patent/DK501289A/da
Priority to DK200401451A priority Critical patent/DK175855B1/da
Application granted granted Critical
Publication of DK175813B1 publication Critical patent/DK175813B1/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
DK198905012A 1988-02-11 1989-10-10 Mutantprotease til anvendelse i vaskemidler, DNA-sekvens, som koder for denne, ekspressionsvektor med en sådan sekvens DK175813B1 (da)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DK200401451A DK175855B1 (da) 1988-02-11 2004-09-23 Mutantprotease og protease til anvendelse i vaskemidler, DNA-sekvens, der koder for en sådan protease, ekspressionsvektor, der omfatter denne DNA-sekvens, prokaryotisk værtsstamme transformeret med en sådan vektor, fremgangsmåde til.....

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP88200255 1988-02-11
EP88200255 1988-02-11
NL8900005 1989-01-03
PCT/NL1989/000005 WO1989007642A1 (en) 1988-02-11 1989-02-13 Novel proteolytic enzymes and their use in detergents

Publications (3)

Publication Number Publication Date
DK501289D0 DK501289D0 (da) 1989-10-10
DK501289A DK501289A (da) 1989-12-07
DK175813B1 true DK175813B1 (da) 2005-03-07

Family

ID=8199752

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198905012A DK175813B1 (da) 1988-02-11 1989-10-10 Mutantprotease til anvendelse i vaskemidler, DNA-sekvens, som koder for denne, ekspressionsvektor med en sådan sekvens

Country Status (21)

Country Link
US (1) US5336611A (lv)
EP (3) EP0571049B2 (lv)
JP (1) JP2849141B2 (lv)
KR (1) KR970005249B1 (lv)
CN (1) CN1056187C (lv)
AT (1) ATE250669T1 (lv)
AU (1) AU629814B2 (lv)
BG (1) BG60566B1 (lv)
BR (1) BR8905580A (lv)
CA (1) CA1340366C (lv)
DE (2) DE68929484T3 (lv)
DK (1) DK175813B1 (lv)
ES (1) ES2061929T5 (lv)
FI (1) FI105481B (lv)
GR (1) GR3021934T3 (lv)
IE (2) IE940164L (lv)
LT (1) LT3963B (lv)
LV (1) LV10652B (lv)
PT (1) PT89702B (lv)
RU (1) RU2069695C1 (lv)
WO (1) WO1989007642A1 (lv)

Families Citing this family (125)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5185258A (en) 1984-05-29 1993-02-09 Genencor International, Inc. Subtilisin mutants
JP2731407B2 (ja) * 1987-04-03 1998-03-25 アムジエン・インコーポレーテツド 新規なプロテアーゼ酵素
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
BR9006827A (pt) * 1989-06-26 1991-08-06 Unilever Nv Composicoes detergentes enzimaticas
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
EP0414297B1 (en) * 1989-08-11 1996-10-16 Gist-Brocades N.V. Efficient production of mutant proteases
US5352603A (en) * 1989-08-31 1994-10-04 Kali-Chemie Ag Highly alkaline proteases
DE4023458A1 (de) * 1989-08-31 1991-03-07 Kali Chemie Ag Neue hochalkalische proteasen
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
DK97190D0 (da) * 1990-04-19 1990-04-19 Novo Nordisk As Oxidationsstabile detergentenzymer
ES2091280T3 (es) * 1990-11-14 1996-11-01 Procter & Gamble Composicion detergente liquida que contiene lipasa y proteasa.
DK271490D0 (da) * 1990-11-14 1990-11-14 Novo Nordisk As Detergentkomposition
US5733473A (en) * 1990-11-14 1998-03-31 The Procter & Gamble Company Liquid detergent composition containing lipase and protease
GB9027836D0 (en) * 1990-12-21 1991-02-13 Unilever Plc Enzymes and enzymatic detergent compositions
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
DE4106525A1 (de) * 1991-03-01 1992-09-10 Solvay Enzymes Gmbh & Co Kg Verfahren zur verbesserung der stabilitaet von hochalkalischen proteasen
US5863783A (en) * 1991-03-27 1999-01-26 Gist-Brocades, N.V. Cloning and expression of DNA molecules encoding arabinan-degrading enzymes of fungal origin
DK58391D0 (da) * 1991-04-03 1991-04-03 Novo Nordisk As Hidtil ukendte proteaser
WO1992019729A1 (en) * 1991-05-01 1992-11-12 Novo Nordisk A/S Stabilized enzymes and detergent compositions
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US5510048A (en) * 1991-05-31 1996-04-23 Colgate Palmolive Co. Nonaqueous liquid, phosphate-free, improved autoamatic dishwashing composition containing enzymes
DE4224125A1 (de) * 1991-07-27 1993-01-28 Solvay Enzymes Gmbh & Co Kg Verfahren zur verbesserung der stabilitaet von enzymen und stabilisierte enzyme
US5792623A (en) * 1992-04-06 1998-08-11 Immuno Aktiengesellschaft Method for producing activated blood factors with a protease and a detergent
AT397390B (de) * 1992-04-06 1994-03-25 Immuno Ag Verfahren zur spaltung von proteinen
EP0571014B1 (en) * 1992-05-18 2004-03-31 Genencor International, Inc. Bacteria producing alkaline proteases, and production of these alkaline proteases
ES2296285T3 (es) * 1992-07-17 2008-04-16 Genencor International, Inc. Serin proteasas altamente alcalinas.
DE69334295D1 (de) * 1992-07-23 2009-11-12 Novo Nordisk As MUTIERTE -g(a)-AMYLASE, WASCHMITTEL UND GESCHIRRSPÜLMITTEL
US5989887A (en) * 1992-11-25 1999-11-23 Dsm N.V. Cloning and expression of DNA molecules incoding arabinan-degrading enzymes of fungal origin
DE4304161A1 (de) * 1993-02-12 1994-08-25 Solvay Enzymes Gmbh & Co Kg Verbesserte hochalkalische Proteasen
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
MA23346A1 (fr) 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
US5932532A (en) * 1993-10-14 1999-08-03 Procter & Gamble Company Bleach compositions comprising protease enzyme
BR9407834A (pt) * 1993-10-14 1997-05-13 Procter & Gamble Composições de limpeza contendo protease
BR9407833A (pt) * 1993-10-14 1997-05-13 Procter & Gamble Composições alvejantes compreendendo enzimas protease
ES2364774T3 (es) 1994-02-24 2011-09-14 HENKEL AG & CO. KGAA Enzimas mejoradas y detergentes que las contienen.
DE4411223A1 (de) * 1994-03-31 1995-10-05 Solvay Enzymes Gmbh & Co Kg Verwendung alkalischer Proteasen in gewerblichen Textilwaschverfahren
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
CA2193117C (en) * 1994-06-17 2007-10-30 Roelck Anneke Cuperus Cleaning compositions containing plant cell wall degrading enzymes and their use in cleaning methods
US6066611A (en) * 1994-10-13 2000-05-23 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
US5714373A (en) * 1995-02-27 1998-02-03 Recombinant Biocatalysis, Inc. Thermococcus AV4 and enzymes produced by the same
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
US6682924B1 (en) * 1995-05-05 2004-01-27 Novozymes A/S Protease variants and compositions
DE19530816A1 (de) * 1995-08-23 1997-02-27 Cognis Bio Umwelt Verwendung von mutierter Subtilisin-Protease in kosmetischen Produkten
WO1998020115A1 (en) 1996-11-04 1998-05-14 Novo Nordisk A/S Subtilase variants and compositions
DK0942962T3 (da) * 1996-11-26 2007-05-07 Genencor Int Kemisk modificerede enzymer
MA24811A1 (fr) * 1997-10-23 1999-12-31 Procter & Gamble Compositions de lavage contenant des variantes de proteases multisubstituees
ES2367505T3 (es) 1997-10-23 2011-11-04 Danisco Us Inc. Variantes de proteasa con sustituciones múltiples con carga neta alterada para usar en detergentes.
JP2002500874A (ja) 1998-01-23 2002-01-15 ジェネンコア インターナショナル インコーポレーテッド ペプチド合成のための修飾された酵素およびその使用方法
US6642011B2 (en) 1998-04-15 2003-11-04 Genencor International, Inc. Human protease and use of such protease for pharmaceutical applications and for reducing the allergenicity of non-human proteins
CA2331783C (en) 1998-07-02 2009-12-29 Genencor International, Inc. Chemically modified proteins with a carbohydrate moiety
US6831053B1 (en) 1998-10-23 2004-12-14 The Procter & Gamble Company Bleaching compositions comprising multiply-substituted protease variants
AU772427B2 (en) 1998-11-10 2004-04-29 Genencor International, Inc. Chemically modified mutant serine hydrolases show improved catalytic activity and chiral selectivity
ATE438724T1 (de) 1998-12-21 2009-08-15 Genencor Int Chemisch modifizierte enzymen mit mehrfachgeladenen varianten
KR20000065867A (ko) * 1999-04-09 2000-11-15 손경식 세탁세제용 알칼리성 단백질 분해효소 브이에이피케이, 이를 코딩하는 유전자, 재조합 발현벡터 및 형질전환된 균주
WO2000064485A2 (en) 1999-04-28 2000-11-02 Genencor International, Inc. Specifically targeted catalytic antagonists and uses thereof
US6627744B2 (en) 1999-07-02 2003-09-30 Genencor International, Inc. Synthesis of glycodendrimer reagents
US6727085B2 (en) * 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) * 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
US6541234B1 (en) * 2000-09-11 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
US6541235B1 (en) * 2000-09-29 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
DK200101090A (da) 2001-07-12 2001-08-16 Novozymes As Subtilase variants
DE10153792A1 (de) 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DE10162727A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10162728A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
CA2795814A1 (en) 2001-12-31 2003-07-17 Genencor International, Inc. Proteases producing an altered immunogenic response and methods of making and using the same
MXPA04006811A (es) * 2002-01-16 2004-10-11 Genencor Int Variantes de proteasa substituidas con multiplos.
AU2003210552A1 (en) * 2002-01-16 2003-09-02 Genencor International, Inc. Multiply-substituted protease variants
EP2339017A3 (en) 2002-03-29 2011-10-12 Genencor International, Inc. Enhanced protein expression in bacillus
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
DE10260903A1 (de) * 2002-12-20 2004-07-08 Henkel Kgaa Neue Perhydrolasen
WO2006033668A2 (en) * 2004-04-09 2006-03-30 Genencor International, Inc. Pcka modifications and enhanced protein expression in bacillus
MX2008011092A (es) * 2006-03-02 2008-09-10 Genencor Int Blanqueador activo de superficie y ph dinamico.
EP2059591B1 (en) * 2006-07-18 2012-09-05 Danisco US Inc. Dishwashing composition that contains a protease variant
US20080090745A1 (en) * 2006-10-13 2008-04-17 Fox Bryan P Expression of Streptomyces subtilism inhibitor (SSI) proteins in Bacillus and Streptomyces sp.
CN103667323B (zh) 2007-03-12 2016-02-03 丹尼斯科美国公司 经修饰的蛋白酶
ATE550348T1 (de) 2007-05-10 2012-04-15 Danisco Us Inc Modifiziertes ausscheidungssystem zur erhöhung der expression von polypeptiden in bakterien
WO2008153935A2 (en) 2007-06-06 2008-12-18 Danisco Us, Inc., Genencor Division Methods for improving protein properties
RU2569106C2 (ru) * 2007-06-06 2015-11-20 ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН Способы улучшения эффективности белков
DE102007044415A1 (de) 2007-09-17 2009-03-19 Henkel Ag & Co. Kgaa Leistungsverbesserte Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
US20110147419A1 (en) * 2007-12-14 2011-06-23 Atsushi Tada Pressure accumulation dispenser
KR20100098652A (ko) 2007-12-21 2010-09-08 다니스코 유에스 인크. 바실루스 내에서의 향상된 단백질 생산
US20090233830A1 (en) * 2008-03-14 2009-09-17 Penny Sue Dirr Automatic detergent dishwashing composition
EP2100947A1 (en) * 2008-03-14 2009-09-16 The Procter and Gamble Company Automatic dishwashing detergent composition
KR101683302B1 (ko) * 2008-03-28 2016-12-06 다니스코 유에스 인크. 박테리아 세포 내 로커스 증폭 방법
CA2742992A1 (en) * 2008-11-11 2010-05-20 Danisco Us Inc. Compositions and methods comprising a subtilisin variant
KR20110095254A (ko) 2008-11-11 2011-08-24 다니스코 유에스 인크. 서브틸리신 변이체를 함유하는 조성물 및 방법
JP5662349B2 (ja) * 2009-02-02 2015-01-28 ザ プロクター アンド ギャンブルカンパニー 食器手洗い用液体洗剤組成物
EP2216392B1 (en) * 2009-02-02 2013-11-13 The Procter and Gamble Company Liquid hand dishwashing detergent composition
EP2213713B1 (en) * 2009-02-02 2014-03-12 The Procter and Gamble Company Liquid hand dishwashing detergent composition
EP2216391A1 (en) * 2009-02-02 2010-08-11 The Procter & Gamble Company Liquid hand dishwashing detergent composition
EP2213715A1 (en) * 2009-02-02 2010-08-04 The Procter & Gamble Company Liquid hand dishwashing detergent composition
EP2216390B1 (en) * 2009-02-02 2013-11-27 The Procter and Gamble Company Hand dishwashing method
EP2216393B1 (en) * 2009-02-09 2024-04-24 The Procter & Gamble Company Detergent composition
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
GB0915572D0 (en) 2009-09-07 2009-10-07 Reckitt Benckiser Nv Detergent composition
WO2011036264A1 (en) * 2009-09-25 2011-03-31 Novozymes A/S Use of protease variants
MX339810B (es) * 2010-05-06 2016-06-09 The Procter & Gamble Company * Productos de consumo con variantes de la proteasa.
WO2013001078A1 (en) 2011-06-30 2013-01-03 Novozymes A/S Alpha-amylase variants
CN102277344B (zh) * 2011-08-03 2012-12-12 天津科技大学 一种低温碱性蛋白酶及其制备方法
DE102013219467A1 (de) * 2013-09-26 2015-03-26 Henkel Ag & Co. Kgaa Stabilisierte Inhibitor-Proteasevarianten
CN106068273B (zh) 2013-12-31 2021-01-08 丹尼斯科美国公司 增强的蛋白质表达
DE102014212639A1 (de) * 2014-06-30 2015-12-31 Henkel Ag & Co. Kgaa Reinigungsmittel enthaltend Proteasen
DK3212780T3 (da) * 2014-10-27 2020-03-23 Danisco Us Inc Serinprotease
WO2016099917A1 (en) 2014-12-16 2016-06-23 Danisco Us Inc Enhanced protein expression
WO2016100128A1 (en) 2014-12-19 2016-06-23 Danisco Us Inc Enhanced protein expression
CA2981409A1 (en) 2015-05-08 2016-11-17 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
EP3964575A3 (en) 2015-05-08 2022-09-28 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
CN108473974A (zh) 2015-11-24 2018-08-31 诺维信公司 具有蛋白酶活性的多肽以及编码其的多核苷酸
EP3464538A1 (en) * 2016-05-31 2019-04-10 Novozymes A/S Stabilized liquid peroxide compositions
DE102016210628A1 (de) * 2016-06-15 2017-12-21 Henkel Ag & Co. Kgaa Bacillus gibsonii Protease und Varianten davon
EP3585910B1 (en) 2017-02-24 2024-03-20 Danisco US Inc. Compositions and methods for increased protein production in bacillus licheniformis
US11879127B2 (en) 2017-08-23 2024-01-23 Danisco Us Inc. Methods and compositions for efficient genetic modifications of Bacillus licheniformis strains
JP7218985B2 (ja) 2017-09-13 2023-02-07 ダニスコ・ユーエス・インク バチルス属(Bacillus)におけるタンパク質産生の増加のための改変5’-非翻訳領域(UTR)配列
EP3703661A1 (en) 2017-11-02 2020-09-09 Danisco US Inc. Freezing point depressed solid matrix compositions for melt granulation of enzymes
DK3735478T5 (da) 2018-01-03 2024-08-26 Danisco Us Inc Mutante og genmodificerede bacillus-celler og fremgangsmåder dertil til forøget proteinproduktion
US20200032178A1 (en) * 2018-07-27 2020-01-30 The Procter & Gamble Company Water-soluble unit dose articles comprising water-soluble fibrous structures and particles
JP2023524334A (ja) 2020-01-15 2023-06-12 ダニスコ・ユーエス・インク バチルス・リケニフォルミス(bacillus licheniformis)における強化したタンパク質産生のための組成物及び方法
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2023023644A1 (en) 2021-08-20 2023-02-23 Danisco Us Inc. Polynucleotides encoding novel nucleases, compositions thereof and methods thereof for eliminating dna from protein preparations
WO2024186819A1 (en) * 2023-03-06 2024-09-12 Danisco Us Inc. Subtilisin variants and methods of use

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1240058A (en) * 1968-04-12 1971-07-21 Procter & Gamble Enzyme-containing detergent compositions
GB1353317A (en) 1970-03-03 1974-05-15 Koninklijke Gist Spiritus Enzyme-polymer complexes
GB1519148A (en) * 1974-11-19 1978-07-26 Gist Brocades Nv Compositions of matter
US4318818A (en) 1979-11-09 1982-03-09 The Procter & Gamble Company Stabilized aqueous enzyme composition
JPS6055118B2 (ja) 1982-02-08 1985-12-03 昭和電工株式会社 新規な細菌アルカリプロテア−ゼ及びその製造方法
GB8311314D0 (en) 1983-04-26 1983-06-02 Unilever Plc Aqueous enzyme-containing compositions
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
NZ208612A (en) * 1983-06-24 1991-09-25 Genentech Inc Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations
US4511490A (en) * 1983-06-27 1985-04-16 The Clorox Company Cooperative enzymes comprising alkaline or mixtures of alkaline and neutral proteases without stabilizers
DK263584D0 (da) 1984-05-29 1984-05-29 Novo Industri As Enzymholdige granulater anvendt som detergentadditiver
WO1986002053A1 (en) 1984-10-05 1986-04-10 Zahnradfabrik Friedrichshafen Ag Steering servo-system, especially for motorized vehicles
DE3685769T2 (de) 1985-04-15 1993-01-21 Procter & Gamble Fluessige reinigungsmittel mit einer oberflaechenaktiven verbindung, einem proteolytischen enzym und borsaeure.
NZ216792A (en) 1985-07-26 1989-04-26 Colgate Palmolive Co Stabilised,fabric-softening built detergent compositions containing enzymes and swelling bentonite clay
US4771003A (en) 1985-10-22 1988-09-13 Genex Corporation Heat stable alkaline proteases produced by a bacillus
JP2599946B2 (ja) * 1986-01-15 1997-04-16 アムジェン,インコーポレイテツド ズブチリシン類似体
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
EP0260299A4 (en) * 1986-02-12 1988-11-24 Genex Corp MUTAGENESIS AND SCREENING PROCESS AND PRODUCT OBTAINED.
EG18543A (en) 1986-02-20 1993-07-30 Albright & Wilson Protected enzyme systems
ATE116365T1 (de) * 1986-04-30 1995-01-15 Genencor Int Mutante einer nicht menschlichen carbonyl- hydrolase, für diese kodierende dns-sequenzen und vektoren und durch diese vektoren transformierte wirte.
EP0896062A3 (en) * 1987-02-27 1999-04-07 Genencor International, Inc. Transformation of alkalophilic bacillus strains
ATE184316T1 (de) * 1987-04-06 1999-09-15 Novo Nordisk As Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer

Also Published As

Publication number Publication date
AU3050189A (en) 1989-09-06
DK501289D0 (da) 1989-10-10
IE940164L (en) 1989-08-11
GR3021934T3 (en) 1997-03-31
DE68929484T2 (de) 2004-07-22
LT3963B (en) 1996-05-27
LV10652A (lv) 1995-04-20
EP0571049B2 (en) 2008-09-03
CN1056187C (zh) 2000-09-06
BG89942A (bg) 1993-12-24
EP0328229B1 (en) 1994-01-19
PT89702B (pt) 1994-04-29
RU2069695C1 (ru) 1996-11-27
JP2849141B2 (ja) 1999-01-20
ATE250669T1 (de) 2003-10-15
FI105481B (fi) 2000-08-31
AU629814B2 (en) 1992-10-15
EP1160327A3 (en) 2002-11-06
FI894799A0 (fi) 1989-10-10
EP0328229A1 (en) 1989-08-16
DE68929484T3 (de) 2009-06-25
KR900700597A (ko) 1990-08-16
DE68912359D1 (de) 1994-03-03
KR970005249B1 (ko) 1997-04-14
BR8905580A (pt) 1990-08-14
DE68912359T2 (de) 1994-06-09
DE68912359T3 (de) 1998-01-08
PT89702A (pt) 1989-10-04
LV10652B (en) 1995-08-20
EP0571049B1 (en) 2003-09-24
LTIP1650A (en) 1995-07-25
EP0328229B2 (en) 1996-10-30
JPH02503986A (ja) 1990-11-22
CN1036602A (zh) 1989-10-25
CA1340366C (en) 1999-02-02
WO1989007642A1 (en) 1989-08-24
EP0571049A1 (en) 1993-11-24
DE68929484D1 (de) 2003-10-30
IE890446L (en) 1989-08-11
IE63248B1 (en) 1995-04-05
BG60566B1 (en) 1995-08-28
EP1160327A2 (en) 2001-12-05
US5336611A (en) 1994-08-09
DK501289A (da) 1989-12-07
ES2061929T5 (es) 1997-03-16
ES2061929T3 (es) 1994-12-16

Similar Documents

Publication Publication Date Title
DK175813B1 (da) Mutantprotease til anvendelse i vaskemidler, DNA-sekvens, som koder for denne, ekspressionsvektor med en sådan sekvens
US5324653A (en) Recombinant genetic means for the production of serine protease muteins
KR100832764B1 (ko) 알카리 프로테아제
US6271012B1 (en) Protease muteins and their use in detergents
JP3922391B2 (ja) 改良酵素とこれを含有する洗剤
DK175697B1 (da) Muterede subtilisin-gener
US8183024B2 (en) Compositions and methods comprising a subtilisin variant
CN116286218A (zh) 包括蛋白酶变体的液体清洁组合物
JP2002272488A (ja) 変異したスブチリシンプロテアーゼ
EP0506448A1 (en) Alpha-lytic protease cleaning compositions
DK175855B1 (da) Mutantprotease og protease til anvendelse i vaskemidler, DNA-sekvens, der koder for en sådan protease, ekspressionsvektor, der omfatter denne DNA-sekvens, prokaryotisk værtsstamme transformeret med en sådan vektor, fremgangsmåde til.....
MXPA99004150A (en) Subtilase variants and compositions

Legal Events

Date Code Title Description
PUP Patent expired