DK175460B1 - Fremgangsmåde til fremstilling af et pattedyrpolypeptid - Google Patents

Fremgangsmåde til fremstilling af et pattedyrpolypeptid Download PDF

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Publication number
DK175460B1
DK175460B1 DK414486A DK414486A DK175460B1 DK 175460 B1 DK175460 B1 DK 175460B1 DK 414486 A DK414486 A DK 414486A DK 414486 A DK414486 A DK 414486A DK 175460 B1 DK175460 B1 DK 175460B1
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Denmark
Prior art keywords
dna
sequence
fragment
aspergillus
polypeptide
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DK414486A
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Danish (da)
English (en)
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DK414486A (da
DK414486D0 (da
Inventor
Kirk J Hayenga
Randy M Berka
Daniel Cullen
Gregory L Gray
Virgil B Lawlis
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Genencor Inc
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27118452&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK175460(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genencor Inc filed Critical Genencor Inc
Publication of DK414486D0 publication Critical patent/DK414486D0/da
Publication of DK414486A publication Critical patent/DK414486A/da
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2428Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478Aspartic endopeptidases (3.4.23)
    • C12N9/6481Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/50Fusion polypeptide containing protease site
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/61Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
DK414486A 1985-08-29 1986-08-29 Fremgangsmåde til fremstilling af et pattedyrpolypeptid DK175460B1 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US77137485A 1985-08-29 1985-08-29
US77137485 1985-08-29
US88222486A 1986-07-07 1986-07-07
US88222486 1986-07-07

Publications (3)

Publication Number Publication Date
DK414486D0 DK414486D0 (da) 1986-08-29
DK414486A DK414486A (da) 1987-03-01
DK175460B1 true DK175460B1 (da) 2004-11-01

Family

ID=27118452

Family Applications (1)

Application Number Title Priority Date Filing Date
DK414486A DK175460B1 (da) 1985-08-29 1986-08-29 Fremgangsmåde til fremstilling af et pattedyrpolypeptid

Country Status (10)

Country Link
EP (2) EP0625577A1 (fr)
JP (1) JPH08280388A (fr)
AT (1) ATE117020T1 (fr)
AU (1) AU607398B2 (fr)
CA (1) CA1341532C (fr)
DE (1) DE3650202T3 (fr)
DK (1) DK175460B1 (fr)
HK (1) HK1003579A1 (fr)
IE (2) IE65794B1 (fr)
NZ (1) NZ217373A (fr)

Families Citing this family (127)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4885249A (en) * 1984-12-05 1989-12-05 Allelix, Inc. Aspergillus niger transformation system
GB8508800D0 (en) * 1985-04-04 1985-05-09 Glaxo Group Ltd Microbiological process
US5198345A (en) * 1985-04-15 1993-03-30 Gist-Brocades N.V. Vectors in use in filamentous fungi
WO1986006097A1 (fr) * 1985-04-15 1986-10-23 Allelix, Inc. Activeurs de champignons filamenteux et leur utilisation
US5766912A (en) * 1986-03-17 1998-06-16 Novo Nordisk A/S Humicola lipase produced in aspergillus
US5536661A (en) * 1987-03-10 1996-07-16 Novo Nordisk A/S Process for the production of protein products in aspergillus
DK122686D0 (da) * 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5780292A (en) * 1987-04-29 1998-07-14 Alko Group Ltd. Production of phytate degrading enzymes in trichoderma
GB8610600D0 (en) * 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
US5610034A (en) * 1987-04-29 1997-03-11 Alko Group Ltd. Immunoglobulin production by trichoderma
ATE125865T1 (de) * 1987-08-28 1995-08-15 Novo Nordisk As Rekombinante humicola-lipase und verfahren zur herstellung von rekombinanten humicola-lipasen.
DE3886221T3 (de) * 1987-09-04 2000-12-21 Novo Nordisk As VERFAHREN ZUR HERSTELLUNG VON PROTEINPRODUKTEN IN -i(ASPERGILLUS) UND PROMOTOREN ZUR VERWENDUNG IN -i(ASPERGILLUS).
CA1333777C (fr) * 1988-07-01 1995-01-03 Randy M. Berka Champignons filamenteux deficients en proteinase aspartique
DK0429628T3 (da) * 1989-06-16 1999-05-31 Genencor Int DNA-sekvenser, vektorer og fusionspeptider til forøgelse af sekretion af ønskede polypeptider fra filamentøse fungi
FR2649120B1 (fr) * 1989-06-30 1994-01-28 Cayla Nouvelle souche et ses mutants de champignons filamenteux, procede de production de proteines recombinantes a l'aide de ladite souche et souches et proteines obtenues selon ce procede
WO1991013971A1 (fr) * 1990-03-13 1991-09-19 Hawaii Biotechnology Group, Inc. SYSTEME D'EXPRESSION A $i(NEUROSPORA)
AU657911B2 (en) * 1990-07-16 1995-03-30 Roal Oy Immunoglobulin production by (Trichoderma)
ATE123524T1 (de) * 1991-03-22 1995-06-15 Novo Nordisk As Herstellungsverfahren für chymosin.
DE69231137T2 (de) * 1991-03-22 2001-02-15 Novo Nordisk As Verfahren zur Herstellung von Peroxidasen in einem Aspergillusstamm
DK36592D0 (da) * 1992-03-20 1992-03-20 Novo Nordisk As Fremgangsmaade til fremstilling af proteiner
PT655890E (pt) * 1992-07-31 2005-05-31 Ab Enzymes Gmbh Celulas recombinantes, construcoes de adn, vectores e metodos para expressao de enzimas degradantes de fitato em proporcoes desejadas
DE69432543T2 (de) * 1993-07-23 2003-12-24 Dsm Nv Selektionmarker-genfreie rekombinante Stämme: Verfahren zur ihrer Herstellung und die Verwendung dieser Stämme
US7816129B2 (en) 1994-07-29 2010-10-19 Ab Enzymes Gmbh Production and secretion of proteins of bacterial origin in filamentous fungi
US5935836A (en) * 1994-07-29 1999-08-10 Rohm Enzyme Finland Oy Actinomadura xylanase sequences and methods of use
US6300114B1 (en) * 1994-07-29 2001-10-09 Rohm Enzyme Finland Oy Sequences of xylanase and xylanase expression vectors
US5700686A (en) * 1995-06-06 1997-12-23 Iogen Corporation Protease-treated and purified cellulase compositions and methods for reducing backstaining during enzymatic stonewashing
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US6265204B1 (en) 1997-01-17 2001-07-24 Genencor International, Inc. DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
DE69922978T2 (de) 1998-10-06 2005-12-08 Emalfarb, Mark Aaron, Jupiter Transformationsystem in filamentösen fungiziden chrysosporium-wirtszellen
US7189538B2 (en) * 1999-03-24 2007-03-13 Genencor International, Inc. Hyphal growth in fungi
AU7398100A (en) * 1999-09-13 2001-04-17 Concordia University A multifunctional system for the efficient manipulation of protein expression infilamentous fungi and method using same
AU2002240882A1 (en) * 2000-12-29 2002-07-16 Rhein Biotech Gesellschaft Fur Neue Biotechnologische Prozesse Und Produkte Mbh Method for producing heterologous proteins in a homothallic fungus of the sordariaceae family
ES2432967T3 (es) 2001-03-22 2013-12-05 Novo Nordisk Health Care Ag Derivados del Factor VII de coagulación
RU2325401C2 (ru) 2001-09-27 2008-05-27 Ново Нордиск Хелт Кэр Аг Полипептид фактора коагуляции vii человека, его получение и применение
DK1495055T3 (da) 2002-04-18 2013-11-11 Genencor Int Produktion af funktionelle antistoffer i filamentøse svampe
ES2403933T3 (es) 2002-09-10 2013-05-22 Genencor International, Inc. Inducción de la expresión génica utilizando una mezcla de azúcares a alta concentración
WO2004072225A2 (fr) 2003-02-12 2004-08-26 Ramot At Tel Aviv University Ltd. Champignons transgeniques exprimant bcl-2 et procedes d'utilisation de bcl-2 ou de parties de celui-ci aux fins d'amelioration de la production, survie, longevite, resistance au stress de la biomasse et de la pathogenicite des champignons
ATE547519T1 (de) 2003-09-09 2012-03-15 Novo Nordisk Healthcare Ag Gerinnungsfaktor-vii-polypeptide
KR20120099303A (ko) 2003-11-06 2012-09-07 다니스코 유에스 인크. 프로테아제 저해자 및 그 변이체의 사상균 내에서의 발현
CN102174490B (zh) 2003-11-21 2016-09-28 丹尼斯科美国公司 颗粒淀粉水解酶在木霉菌中的表达和从颗粒淀粉底物产生葡萄糖的方法
WO2005118800A1 (fr) 2004-05-27 2005-12-15 Genencor International, Inc. Expression heterologue d'une alpha-amylase de l'aspergillus kawachi stable aux acides et applications pour l'hydrolyse de l'amidon granule
US7332319B2 (en) 2004-05-27 2008-02-19 Genencor International, Inc. Heterologous alpha amylase expression in Aspergillus
US7413887B2 (en) 2004-05-27 2008-08-19 Genecor International, Inc. Trichoderma reesei glucoamylase and homologs thereof
AU2005249422B2 (en) 2004-05-27 2011-03-17 Genencor International, Inc. Acid-stable alpha amylases having granular starch hydrolyzing activity and enzyme compositions
US7429476B2 (en) 2004-12-30 2008-09-30 Genencor International, Inc. Acid fungal proteases
ZA200706858B (en) 2005-02-18 2008-10-29 Genencor Int Polypeptides having alpha-amylase and granular starch hydrolyzing activity
WO2006110677A2 (fr) 2005-04-12 2006-10-19 Genencor International, Inc. Mutants a genes inactives presentant une production de proteine modifiee
EP1926817A2 (fr) 2005-09-14 2008-06-04 Novo Nordisk Health Care AG Polypeptides du facteur vii de coagulation humain
US8198046B2 (en) 2006-07-11 2012-06-12 Danisco Us Inc. KEX2 cleavage regions of recombinant fusion proteins
US20080026376A1 (en) 2006-07-11 2008-01-31 Huaming Wang KEX2 cleavage regions of recombinant fusion proteins
US7968691B2 (en) 2006-08-23 2011-06-28 Danisco Us Inc. Pullulanase variants with increased productivity
EP2082047A2 (fr) 2006-08-29 2009-07-29 Danisco US, INC., Genencor Division Compositions et procédés de production améliorée de protéines
BRPI0719181A2 (pt) 2006-10-10 2014-08-05 Danisco Us Inc Genencor Div Variantes de glucoamilase com propriedades alteradas
US8680252B2 (en) 2006-12-10 2014-03-25 Dyadic International (Usa), Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
US20080220498A1 (en) 2007-03-06 2008-09-11 Cervin Marguerite A Variant Buttiauxella sp. phytases having altered properties
US8143046B2 (en) 2007-02-07 2012-03-27 Danisco Us Inc., Genencor Division Variant Buttiauxella sp. phytases having altered properties
JP5167286B2 (ja) 2007-03-14 2013-03-21 ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン トリコデルマ・レセイ(TRICHODERMAREESEI)α−アミラーゼはマルトース産生酵素である。
WO2008150376A1 (fr) 2007-05-21 2008-12-11 Danisco Us, Inc., Genencor Division Utilisation d'une séquence de signaux de protéase aspartique (nsp24) pour l'expression d'une protéine hétérologue
DK2152892T3 (da) 2007-05-21 2013-07-22 Danisco Us Inc Genencor Div Fremgangsmåde til indføring af nukleinsyrer i fungale celler
US8551751B2 (en) 2007-09-07 2013-10-08 Dyadic International, Inc. BX11 enzymes having xylosidase activity
EP2195435B1 (fr) 2007-09-12 2012-10-10 Danisco US Inc. Promoteur de trichoderma
JP5594898B2 (ja) 2007-10-09 2014-09-24 ダニスコ・ユーエス・インク 性質が変えられたグルコアミラーゼ変異種
US8592194B2 (en) 2007-10-09 2013-11-26 Danisco Us Inc. Glucoamylase variants with altered properties
HUE050328T2 (hu) 2007-11-20 2020-11-30 Danisco Us Inc Glükoamiláz változatok megváltoztatott tulajdonságokkal
AU2008334945C1 (en) 2007-12-13 2013-09-05 Danisco Us Inc. Compositions and methods for producing isoprene
US8318451B2 (en) 2008-01-02 2012-11-27 Danisco Us Inc. Process of obtaining ethanol without glucoamylase using Pseudomonas saccharophila G4-amylase variants thereof
DK2268806T3 (da) 2008-02-11 2013-07-15 Danisco Us Inc Enzym med mikrobiel lyseaktivitet fra Trichoderma reesei
DK2283124T3 (en) 2008-04-18 2016-09-05 Danisco Us Inc BUTTIAUXELLA SP. PHYTASE VARIANTS
MX2010011706A (es) 2008-04-23 2011-03-15 Danisco Us Inc Variantes de isopreno-sintasa para produccion microbiana mejorada de isopreno.
JP2011526790A (ja) 2008-07-02 2011-10-20 ダニスコ・ユーエス・インク 分離された条件下および/または安全操業条件下における、c5炭化水素を含まないイソプレン組成物及びその製造方法
US8361762B2 (en) 2008-09-15 2013-01-29 Danisco Us Inc. Increased isoprene production using the archaeal lower mevalonate pathway
CA2737195A1 (fr) 2008-09-15 2010-03-18 The Goodyear Tire & Rubber Company Conversion de derives de prenyle en isoprene
BRPI0918453A2 (pt) 2008-09-15 2019-12-17 Danisco Us Inc sistemas usando cultura de células para a produção de isopreno
SG169652A1 (en) 2008-09-15 2011-04-29 Danisco Us Inc Reduction of carbon dioxide emission during isoprene production by fermentation
DK2358873T4 (en) 2008-12-15 2018-05-28 Danisco Us Inc HYBRID ALPHA AMYLASER
EP2382312A2 (fr) 2008-12-30 2011-11-02 Danisco US Inc. Procédé de fabrication d'isoprène et d'un co-produit
CN102656265A (zh) 2009-04-23 2012-09-05 丹尼斯科美国公司 异戊二烯合酶的三维结构及其用于产生变体的用途
DK2432875T3 (en) 2009-05-19 2016-12-19 Dupont Nutrition Biosci Aps Amylasepolypeptide
TW201412988A (zh) 2009-06-17 2014-04-01 Danisco Us Inc 使用dxp及mva途徑之改良之異戊二烯製造
TWI434921B (zh) 2009-06-17 2014-04-21 Danisco Us Inc 從生物異戊二烯組合物製造燃料成分之方法及系統
KR101870378B1 (ko) 2009-07-17 2018-06-25 릭스하스피탈렛 보체 활성화의 억제제로서의 masp 이소형
CN102712915B (zh) 2009-08-19 2014-12-10 丹尼斯科美国公司 具有改良比活和/或热稳定性的葡糖淀粉酶的组合变体
CN107299123A (zh) 2009-08-19 2017-10-27 杜邦营养生物科学有限公司 葡糖淀粉酶的变体
US9096843B2 (en) 2009-12-01 2015-08-04 Novo Nordisk A/S Peptidyl α-hydroxyglycine α-amidating lyases
EP2542569B1 (fr) 2010-03-05 2020-09-16 Omeros Corporation Molécules chimériques inhibitrices d'activation du complément
ES2681692T3 (es) 2010-03-29 2018-09-14 Dupont Nutrition Biosciences Aps Polipéptidos que tienen actividad transgalactosilante
WO2011160081A1 (fr) 2010-06-17 2011-12-22 Danisco Us Inc. Compositions de carburant contenant des dérivés d'isoprène
EP2595488B1 (fr) * 2010-07-21 2019-12-04 Novozymes A/S Procédé pour préparer une produit de boulangerie avec une amylase anti-rassissement et une peptidase
US20120034659A1 (en) 2010-08-06 2012-02-09 Danisco Us Inc. NEUTRAL pH SACCHARIFICATION AND FERMENTATION
US20120045812A1 (en) 2010-08-06 2012-02-23 Danisco Us Inc. PRODUCTION OF ISOPRENE UNDER NEUTRAL pH CONDITIONS
EP2608682B1 (fr) 2010-08-24 2017-12-13 Danisco US Inc. Procédé de préparation d'une barre snack comportant un concentré de protéine de riz à basse température
JP2014502148A (ja) 2010-10-27 2014-01-30 ダニスコ・ユーエス・インク イソプレン生産の向上を目的としたイソプレン合成酵素変異体
JP2014504497A (ja) 2010-12-22 2014-02-24 ダニスコ・ユーエス・インク 2種類のispg酵素を用いてイソプレン生産を向上させる組成物及び方法
EP2655597A1 (fr) 2010-12-22 2013-10-30 Danisco US Inc. Production biologique de sucres pentoses à l'aide de cellules recombinantes
JP2014512829A (ja) 2011-04-29 2014-05-29 ダニスコ・ユーエス・インク デンプンを液化及び糖化して高密度グルコースシロップを調製するための単一pHプロセス
US8951764B2 (en) 2011-08-05 2015-02-10 Danisco Us Inc. Production of isoprenoids under neutral pH conditions
US20130109055A1 (en) 2011-10-31 2013-05-02 Bp Corporation North America Inc. Use of mammalian promoters in filamentous fungi
WO2013067026A1 (fr) 2011-10-31 2013-05-10 Bp Corporation North America Inc. Utilisation de promoteurs végétaux dans des champignons filamenteux
SG10201606195UA (en) 2012-02-09 2016-09-29 Var2 Pharmaceuticals Aps Targeting of chondroitin sulfate glycans
US9163263B2 (en) 2012-05-02 2015-10-20 The Goodyear Tire & Rubber Company Identification of isoprene synthase variants with improved properties for the production of isoprene
BR122021002223B1 (pt) 2012-06-08 2023-03-28 Dupont Nutrition Biosciences Aps Composição compreendendo polipeptídeo com atividade de transgalactosilação
MX2015002099A (es) 2012-08-22 2015-05-11 Dupont Nutrition Biosci Aps Variantes que tienen actividad glucoamilasa.
JP2015532307A (ja) 2012-10-15 2015-11-09 ノヴォ・ノルディスク・ヘルス・ケア・アーゲー 凝固因子viiポリペプチド
WO2014145768A2 (fr) 2013-03-15 2014-09-18 Bp Corporation North America Inc. Utilisation de 5' utr non fongiques dans des champignons filamenteux
BR112016013485A2 (pt) 2013-12-11 2017-10-03 Dupont Nutrition Biosci Aps Método para preparar um produto lácteo tendo teor estável de galacto-oligossacarídeo(s)
EP3915384A1 (fr) 2014-11-07 2021-12-01 DuPont Nutrition Biosciences ApS Cellule hôte recombinante exprimant bêta-galactosidase et/ou une activité de transgalactosylation déficient en cellulase
JP2018504936A (ja) 2015-02-09 2018-02-22 ダニスコ・ユーエス・インク 真菌株および使用方法
CA2986602A1 (fr) 2015-05-22 2016-12-01 Dupont Nutrition Biosciences Aps Acetolactate decarboxylase
US20180148683A1 (en) 2015-07-07 2018-05-31 Danisco Us Inc. Induction of gene expression using a high concentration sugar mixture
ES2835067T3 (es) 2015-09-02 2021-06-21 Dupont Nutrition Biosci Aps Glucósido hidrolasas y su uso en la prevención y/o tratamiento de infección patógena en un animal
WO2018005225A1 (fr) 2016-06-30 2018-01-04 Danisco Us Inc Protéases aspartiques
CA3037083A1 (fr) 2016-09-16 2018-03-22 Dupont Nutrition Biosciences Aps Variants d'acetolactate decarboxylase ayant une activite specifique amelioree
RU2763378C2 (ru) 2016-09-23 2021-12-28 ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС ПРИМЕНЕНИЕ АКТИВНЫХ ПРИ НИЗКОМ ЗНАЧЕНИИ pH АЛЬФА-1,4/1,6-ГЛИКОЗИДГИДРОЛАЗ В КАЧЕСТВЕ КОРМОВОЙ ДОБАВКИ ДЛЯ ЖВАЧНЫХ ЖИВОТНЫХ ДЛЯ УЛУЧШЕНИЯ ПЕРЕВАРИВАНИЯ КРАХМАЛА
CN109790510B (zh) 2016-10-04 2024-04-12 丹尼斯科美国公司 在不存在诱导底物下丝状真菌细胞中的蛋白产生
WO2018093752A1 (fr) 2016-11-15 2018-05-24 Danisco Us Inc. Champignons filamenteux à production améliorée de protéines
CN110505903A (zh) 2017-03-06 2019-11-26 杜邦营养生物科学有限公司 新型真菌岩藻糖苷酶及其在预防和/或治疗动物病原体感染中的用途
US20210278406A1 (en) 2018-07-13 2021-09-09 Varct Diagnostic Aps Isolation of circulating cells of fetal origin using recombinant malaria protein var2csa
CN113784990A (zh) 2019-03-08 2021-12-10 丹尼斯科美国公司 融合多肽
CN113874392A (zh) 2019-03-28 2021-12-31 丹尼斯科美国公司 工程化抗体
JP2023506284A (ja) 2019-12-19 2023-02-15 ビーエーエスエフ ソシエタス・ヨーロピア ファインケミカルを生成する際の空時収率、炭素変換効率、及び炭素基質適応性の増加
CN115135762A (zh) 2019-12-20 2022-09-30 巴斯夫欧洲公司 降低萜烯的毒性和增加微生物的生产潜力
US20230203191A1 (en) 2020-03-30 2023-06-29 Danisco Us Inc Engineered antibodies
US20230174998A1 (en) 2020-04-22 2023-06-08 Danisco Us Inc. Compositions and methods for enhanced protein production in filamentous fungal cells
WO2023003683A2 (fr) 2021-07-19 2023-01-26 Danisco Us Inc. Compositions et procédés pour une production améliorée de protéines dans des cellules fongiques
CN116135979A (zh) * 2021-11-16 2023-05-19 中国科学院天津工业生物技术研究所 一种基于流式细胞术的免涂板操作的菌株工程改造的方法及应用
WO2023102315A1 (fr) 2021-12-01 2023-06-08 Danisco Us Inc. Compositions et procédés pour une production améliorée de protéines dans des cellules fongiques
WO2024015953A1 (fr) 2022-07-15 2024-01-18 Danisco Us Inc. Procédés de production d'anticorps monoclonaux

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4775622A (en) * 1982-03-08 1988-10-04 Genentech, Inc. Expression, processing and secretion of heterologous protein by yeast
NO840200L (no) * 1983-01-28 1984-07-30 Cefus Corp Glukoamylase cdna.
DK601984D0 (da) * 1984-12-14 1984-12-14 Hansens Lab Fremgangsmaade til fremstilling af genprodukter
WO1986006097A1 (fr) * 1985-04-15 1986-10-23 Allelix, Inc. Activeurs de champignons filamenteux et leur utilisation
DK122686D0 (da) * 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
FI872102A (fi) * 1986-06-06 1987-12-07 Panlabs Inc Expressionssystem hos filamentala fungi.

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JPH08280388A (ja) 1996-10-29
EP0215594B1 (fr) 1995-01-11
EP0215594A3 (en) 1988-08-31
DE3650202T2 (de) 1995-06-01
DE3650202D1 (de) 1995-02-23
NZ217373A (en) 1990-10-26
ATE117020T1 (de) 1995-01-15
DK414486A (da) 1987-03-01
DE3650202T3 (de) 2004-06-03
HK1003579A1 (en) 1998-10-30
DK414486D0 (da) 1986-08-29
CA1341532C (fr) 2007-06-19
IE65794B1 (en) 1995-11-15
EP0215594A2 (fr) 1987-03-25
EP0625577A1 (fr) 1994-11-23

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