DK1317539T3 - Fremgangsmåde til målrettet evolution - Google Patents

Fremgangsmåde til målrettet evolution

Info

Publication number
DK1317539T3
DK1317539T3 DK01965462T DK01965462T DK1317539T3 DK 1317539 T3 DK1317539 T3 DK 1317539T3 DK 01965462 T DK01965462 T DK 01965462T DK 01965462 T DK01965462 T DK 01965462T DK 1317539 T3 DK1317539 T3 DK 1317539T3
Authority
DK
Denmark
Prior art keywords
nucleic acid
microcompartments
approach
concentration
increasing
Prior art date
Application number
DK01965462T
Other languages
English (en)
Inventor
Jennifer Lee Ong
Farid Ghadessy
Philipp Holliger
Original Assignee
Medical Res Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Res Council filed Critical Medical Res Council
Application granted granted Critical
Publication of DK1317539T3 publication Critical patent/DK1317539T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Ecology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Electrotherapy Devices (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Soil Working Implements (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
DK01965462T 2000-09-13 2001-09-13 Fremgangsmåde til målrettet evolution DK1317539T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB0022458.4A GB0022458D0 (en) 2000-09-13 2000-09-13 Directed evolution method
US28377101P 2001-04-13 2001-04-13
US28550101P 2001-04-20 2001-04-20
PCT/GB2001/004108 WO2002022869A2 (en) 2000-09-13 2001-09-13 Directed evolution method

Publications (1)

Publication Number Publication Date
DK1317539T3 true DK1317539T3 (da) 2007-03-05

Family

ID=27255886

Family Applications (1)

Application Number Title Priority Date Filing Date
DK01965462T DK1317539T3 (da) 2000-09-13 2001-09-13 Fremgangsmåde til målrettet evolution

Country Status (11)

Country Link
US (3) US7514210B2 (da)
EP (4) EP1317539B1 (da)
JP (2) JP5009481B2 (da)
AT (2) ATE396259T1 (da)
AU (2) AU2001286103B2 (da)
CA (2) CA2585083A1 (da)
DE (2) DE60124272T2 (da)
DK (1) DK1317539T3 (da)
ES (2) ES2304580T3 (da)
GB (1) GB0022458D0 (da)
WO (1) WO2002022869A2 (da)

Families Citing this family (103)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU736836B2 (en) 1997-07-07 2001-08-02 Medical Research Council In vitro sorting method
GB9900298D0 (en) * 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
GB0127564D0 (en) * 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
FR2843404A1 (fr) * 2002-08-07 2004-02-13 Cellectis Methode de determination de l'activite de clivage d'une endonuclease
WO2005003375A2 (en) 2003-01-29 2005-01-13 454 Corporation Methods of amplifying and sequencing nucleic acids
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
EP1641809B2 (en) 2003-07-05 2018-10-03 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
GB0325653D0 (en) * 2003-11-03 2003-12-10 Medical Res Council CST emulsions
EP1801213A3 (en) * 2003-11-03 2007-10-24 Medical Research Council Polymerase
EP1691792A4 (en) * 2003-11-24 2008-05-28 Yeda Res & Dev COMPOSITIONS AND METHODS FOR IN VITRO / I SORTING OF MOLECULAR AND CELLULAR BANKS
WO2005073410A2 (en) 2004-01-28 2005-08-11 454 Corporation Nucleic acid amplification with continuous flow emulsion
US7417133B2 (en) * 2004-02-27 2008-08-26 Institut Pasteur Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
WO2007145612A1 (en) 2005-06-06 2007-12-21 454 Life Sciences Corporation Paired end sequencing
EP1743938A1 (en) * 2005-07-12 2007-01-17 NascaCell IP GmbH Means and methods for displaying polypeptides on cells
DE102005037351B3 (de) * 2005-08-08 2007-01-11 Geneart Ag Verfahren für die kontinuierliche zielgerichtete Evolution von Proteinen in vitro
DE102005037349A1 (de) * 2005-08-08 2007-02-15 Geneart Ag Verfahren für die kontinuierliche zielgerichtete Evolution von Proteinen in vivo
US20100137163A1 (en) 2006-01-11 2010-06-03 Link Darren R Microfluidic Devices and Methods of Use in The Formation and Control of Nanoreactors
EP2481815B1 (en) 2006-05-11 2016-01-27 Raindance Technologies, Inc. Microfluidic devices
US9074242B2 (en) 2010-02-12 2015-07-07 Raindance Technologies, Inc. Digital analyte analysis
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
JP5411694B2 (ja) * 2006-06-19 2014-02-12 ザ ジョンズ ホプキンス ユニバーシティー 油中水型エマルジョン中の微粒子上での単分子ｐｃｒ
WO2008021123A1 (en) 2006-08-07 2008-02-21 President And Fellows Of Harvard College Fluorocarbon emulsion stabilizing surfactants
ES2404665T3 (es) 2006-09-06 2013-05-28 Medical Research Council Polimerasas de ADN que incorporan análogos de nucleótidos marcados con colorante
JP5457184B2 (ja) 2006-09-14 2014-04-02 ディエヌエイ・ポリメラーゼ・テクノロジー・インコーポレイテッド Ｐｃｒ阻害物質の存在下におけるｄｎａ増幅のためのｔａｑポリメラーゼ変異体酵素の使用
ES2627274T3 (es) * 2006-10-23 2017-07-27 Medical Research Council Polimerasa
US7682791B2 (en) * 2006-10-29 2010-03-23 Macevicz Stephen C Method of generating nested sets of double stranded DNA circles
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008103900A2 (en) 2007-02-23 2008-08-28 New England Biolabs, Inc. Selection and enrichment of proteins using in vitro compartmentalization
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
CA2706740C (en) 2007-11-27 2017-01-17 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
US8748727B2 (en) * 2008-01-18 2014-06-10 Tenksolar, Inc. Flat-plate photovoltaic module
US8933320B2 (en) * 2008-01-18 2015-01-13 Tenksolar, Inc. Redundant electrical architecture for photovoltaic modules
GB0806562D0 (en) 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid
WO2009155464A2 (en) * 2008-06-18 2009-12-23 Life Technologies Corporation Mutated and chemically modified thermally stable dna polymerases
EP2315629B1 (en) * 2008-07-18 2021-12-15 Bio-Rad Laboratories, Inc. Droplet libraries
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
WO2010027870A2 (en) * 2008-08-26 2010-03-11 Fluidigm Corporation Assay methods for increased throughput of samples and/or targets
CA2742594C (en) 2008-11-03 2020-03-24 William Bourn Modified type a dna polymerases
EP3415235A1 (en) 2009-03-23 2018-12-19 Raindance Technologies Inc. Manipulation of microfluidic droplets
GB0904957D0 (en) 2009-03-23 2009-05-06 Univ Erasmus Medical Ct Tumour gene profile
AU2010232439C1 (en) 2009-04-02 2017-07-13 Fluidigm Corporation Multi-primer amplification method for barcoding of target nucleic acids
US8003329B2 (en) * 2009-05-12 2011-08-23 Becton Dickinson & Company Molecular counting by color-coded micelles
CN102484154B (zh) * 2009-06-15 2014-12-24 腾克太阳能公司 无光照限制的太阳能电池板
JP2011051943A (ja) * 2009-09-03 2011-03-17 Tosoh Corp タンパク質抽出試薬およびそれを用いたポリメラーゼのスクリーニング方法
WO2011042564A1 (en) 2009-10-09 2011-04-14 Universite De Strasbourg Labelled silica-based nanomaterial with enhanced properties and uses thereof
WO2011056872A2 (en) * 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
US10837883B2 (en) 2009-12-23 2020-11-17 Bio-Rad Laboratories, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
EP2569441B2 (en) * 2010-05-14 2017-09-13 cSens AB Kit and method for measuring the activity of deoxynucleoside kinase using dna-dependent dna polymerase and natural deoxynucleosides
EP3447155A1 (en) 2010-09-30 2019-02-27 Raindance Technologies, Inc. Sandwich assays in droplets
US9315787B2 (en) 2011-01-14 2016-04-19 Kapa Biosystems, Inc. Modified DNA polymerases for improved amplification
EP3412778A1 (en) 2011-02-11 2018-12-12 Raindance Technologies, Inc. Methods for forming mixed droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
SG10201605049QA (en) 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
US9556470B2 (en) 2011-06-02 2017-01-31 Raindance Technologies, Inc. Enzyme quantification
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
DK2718260T3 (da) 2011-06-08 2018-11-19 Life Technologies Corp Design og udvikling af nye detergenter til brug i pcr systemer
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
CA2839964C (en) * 2011-07-28 2019-03-12 F. Hoffmann-La Roche Ag Dna polymerases with improved activity
GB201113430D0 (en) 2011-08-03 2011-09-21 Fermentas Uab DNA polymerases
EP2742151B1 (en) 2011-08-10 2017-10-25 Life Technologies Corporation Polymerase compositions
US11208636B2 (en) 2011-08-10 2021-12-28 Life Technologies Corporation Polymerase compositions, methods of making and using same
GB201120711D0 (en) 2011-12-01 2012-01-11 Univ Erasmus Medical Ct Method for classifying tumour cells
US20130157259A1 (en) * 2011-12-15 2013-06-20 Samsung Electronics Co., Ltd. Method of amplifying dna from rna in sample and use thereof
EP2802666B1 (en) 2012-01-13 2018-09-19 Data2Bio Genotyping by next-generation sequencing
US9701959B2 (en) 2012-02-02 2017-07-11 Invenra Inc. High throughput screen for biologically active polypeptides
SG11201407901PA (en) 2012-05-21 2015-01-29 Fluidigm Corp Single-particle analysis of particle populations
EP3366769B1 (en) * 2012-10-16 2021-02-17 DNA Polymerase Technology, Inc. Inhibition-resistant polymerases
WO2014065760A1 (en) * 2012-10-25 2014-05-01 Agency For Science, Technology And Research Methods for determining resistance against molecules targeting proteins
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
CN106458885B (zh) 2013-10-25 2019-12-10 生命技术公司 用于聚合酶链式反应系统的新颖化合物及其应用
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
EP4122945A1 (en) 2013-12-23 2023-01-25 University of Rochester Methods and compositions for ribosomal synthesis of macrocyclic peptides
WO2015103367A1 (en) 2013-12-31 2015-07-09 Raindance Technologies, Inc. System and method for detection of rna species
CN113403293B (zh) * 2014-12-16 2024-08-16 生命技术公司 聚合酶组合物和制造与使用其的方法
WO2016183294A1 (en) 2015-05-12 2016-11-17 Dna Polymerase Technology, Inc. Mutant polymerases and uses thereof
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
EP3390658B1 (en) 2015-12-16 2022-08-03 Standard BioTools Inc. High-level multiplex amplification
EP3405578B1 (en) 2016-01-19 2022-03-16 Board of Regents, The University of Texas System Thermostable reverse transcriptase
EP3497227A4 (en) 2016-08-09 2020-10-07 Integrated DNA Technologies, Inc. RNASE-H MUTANTS IN AN EMULSION
CN108265039B (zh) * 2016-12-30 2020-07-14 天津强微特生物科技有限公司 一种突变TaqDNA聚合酶及其纯化方法
WO2018209092A1 (en) * 2017-05-10 2018-11-15 Board Of Regents, The University Of Texas System Methods and devices related to amplifying nucleic acid at a variety of temperatures
WO2018232282A1 (en) * 2017-06-15 2018-12-20 Board Of Regents, The University Of Texas System Methods and platform for screening and selecting metabolites and their receptors
LT6615B (lt) 2017-09-12 2019-04-25 Vilniaus Universitetas N4-modifikuoti citidino nukleotidai ir jų panaudojimas
US11866785B2 (en) 2017-10-27 2024-01-09 Board Of Regents, The University Of Texas System Tumor specific antibodies and T-cell receptors and methods of identifying the same
US10961518B2 (en) 2018-01-19 2021-03-30 Bio-Rad Laboratories, Inc. Mutant DNA polymerases
LT6742B (lt) * 2018-10-10 2020-07-10 Vilnius University Katalizinio biomolekulinio aktyvumo įrašymas į dnr seką
WO2020185705A1 (en) * 2019-03-10 2020-09-17 Abclonal Science, Inc. Mutant taq polymerase for amplification in increased salt concentration or body fluids
US11613738B2 (en) * 2019-05-14 2023-03-28 Abclonal Science, Inc. Mutant Taq polymerase resistant to inhibition of amplification in the presence of cyanine dye
CN110218776A (zh) * 2019-06-14 2019-09-10 苏州叠代生物科技有限公司 Pcr油相组合物及其制备方法
WO2021025623A1 (en) * 2019-08-08 2021-02-11 Agency For Science, Technology And Research Method of detecting protein-protein interactions
CN111621482B (zh) * 2020-06-30 2022-04-29 浙江工业大学 一种草铵膦脱氢酶突变体、基因工程菌及一锅法多酶同步定向进化方法
CN114480327B (zh) * 2020-10-26 2024-01-16 厦门大学 一种Taq DNA聚合酶突变体
CN112695019B (zh) * 2021-03-23 2021-06-25 翌圣生物科技（上海）有限公司 逆转录酶突变体及其应用
WO2024009873A1 (ja) * 2022-07-08 2024-01-11 東洋紡株式会社 逆転写活性を有する核酸ポリメラーゼ

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5290479A (en) * 1986-12-09 1994-03-01 Phillips Petroleum Company Surfactant system of polyethoxylated compounds and glyceride compounds
GB9107628D0 (en) * 1991-04-10 1991-05-29 Moonbrook Limited Preparation of diagnostic agents
JPH06509473A (ja) * 1991-08-10 1994-10-27 メディカル・リサーチ・カウンシル 細胞個体群の処理
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5910408A (en) * 1995-06-07 1999-06-08 The General Hospital Corporation Catalytic DNA having ligase activity
US6083693A (en) * 1996-06-14 2000-07-04 Curagen Corporation Identification and comparison of protein-protein interactions that occur in populations
WO1998013502A2 (en) * 1996-09-27 1998-04-02 Icos Corporation Method to identify compounds for disrupting protein/protein interactions
WO1998023733A2 (en) * 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
CA2196496A1 (en) * 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
AU736836B2 (en) * 1997-07-07 2001-08-02 Medical Research Council In vitro sorting method
WO2000004139A1 (en) * 1998-07-17 2000-01-27 Mirus Corporation Micellar systems
GB9818432D0 (en) * 1998-08-24 1998-10-21 Bioline Limited Thermostable DNA Polymerase
GB9900298D0 (en) * 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
GB0114854D0 (en) * 2001-06-18 2001-08-08 Medical Res Council Selective gene amplification
GB0127564D0 (en) * 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions

Also Published As

Publication number Publication date
WO2002022869A2 (en) 2002-03-21
EP1964932A2 (en) 2008-09-03
EP1964932A3 (en) 2009-05-20
JP5009481B2 (ja) 2012-08-22
EP1317539A2 (en) 2003-06-11
EP1505151A2 (en) 2005-02-09
AU2001286103B2 (en) 2006-08-10
JP2007190027A (ja) 2007-08-02
US20090246853A1 (en) 2009-10-01
EP1317539B1 (en) 2006-11-02
DE60124272D1 (de) 2006-12-14
EP1806406A3 (en) 2009-07-29
US20040005594A1 (en) 2004-01-08
AU8610301A (en) 2002-03-26
DE60134181D1 (de) 2008-07-03
ATE396259T1 (de) 2008-06-15
CA2421963A1 (en) 2002-03-21
US7514210B2 (en) 2009-04-07
GB0022458D0 (en) 2000-11-01
CA2585083A1 (en) 2002-03-21
EP1505151A3 (en) 2005-05-11
WO2002022869A3 (en) 2002-10-17
US20080166772A1 (en) 2008-07-10
CA2421963C (en) 2013-04-09
ES2274901T3 (es) 2007-06-01
EP1505151B1 (en) 2008-05-21
DE60124272T2 (de) 2007-05-03
EP1806406A2 (en) 2007-07-11
ATE344320T1 (de) 2006-11-15
ES2304580T3 (es) 2008-10-16
JP2004508834A (ja) 2004-03-25

Similar Documents

Publication Publication Date Title
DK1317539T3 (da) Fremgangsmåde til målrettet evolution
CY1111537T1 (el) Μεθοδος διαχωρισμου in vitro
DK1446504T3 (da) Emulsionssammensætninger
DK1522581T3 (da) Optisk sorteringsmetode
EA200300284A1 (ru) Способ амплификации нуклеиновых кислот
ATE318327T1 (de) Fluoreszenz-donor-akzeptor paar
KR910008135A (ko) 단백질 분해 효소를 사용하지 않는 핵산의 추출 및 pcr 증폭방법
DE69834056D1 (de) De novo oder "universaler" sequenzierungs-array
EA200300647A1 (ru) Изотермическая амплификация нуклеиновых кислот на твердой подложке
BR9911396A (pt) Estabilização de enzima por meio de tensoativos catiÈnicos
ES2087153T3 (es) Ensayos mejorados de hibridacion de acidos nucleicos para hbv que emplean la amplificacion.
ATE309336T1 (de) Umkehrbare modifikation von thermostabilen enzymen
GB2369592A (en) Reaction system for performing in the amplification of nucleic acids
ATE205260T1 (de) Hybridisierungsverfahren in dem überschüssige sonden abgebaut werden
DK19988D0 (da) Fremgangsmaade til fremstilling af optisk aktive cyanhydriner
DE69321112D1 (de) Elektrochemische Behandlung von Nukleinsäure - enthaltenden Lösungen
DK0763133T3 (da) Fremgangsmåde til at detektereen målnucleinsyre
EP1170383A3 (en) Oligonucleotides and method for detection of small round structured virus (SRSV) RNA
DE60127244D1 (de) Spezifische multiplex-analyse von nukleinsäuren
KR920014935A (ko) 사슬 치환에 의한 증폭방법
ES2188357B1 (es) Metodo para la amplificacion mediante transcripcion en reverso acoplada a una reaccion de nested o semi-nested pcr en un solo paso, de un arn diana, y sus aplicaciones en la identificacion de enterovirus y en la discriminacion entre poliovirus y enterovirus no-polio.
EP1321530A3 (en) Method for detecting Escherichia coli