ATE396259T1 - Methode zur zielgerichteten evolution - Google Patents

Methode zur zielgerichteten evolution

Info

Publication number
ATE396259T1
ATE396259T1 AT04078077T AT04078077T ATE396259T1 AT E396259 T1 ATE396259 T1 AT E396259T1 AT 04078077 T AT04078077 T AT 04078077T AT 04078077 T AT04078077 T AT 04078077T AT E396259 T1 ATE396259 T1 AT E396259T1
Authority
AT
Austria
Prior art keywords
nucleic acid
microcompartments
concentration
increasing
amplification
Prior art date
Application number
AT04078077T
Other languages
English (en)
Inventor
Philipp Holliger
Jennifer Lee Ong
Farid Ghadessy
Original Assignee
Medical Res Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Res Council filed Critical Medical Res Council
Application granted granted Critical
Publication of ATE396259T1 publication Critical patent/ATE396259T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Ecology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Electrotherapy Devices (AREA)
  • Soil Working Implements (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
AT04078077T 2000-09-13 2001-09-13 Methode zur zielgerichteten evolution ATE396259T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0022458.4A GB0022458D0 (en) 2000-09-13 2000-09-13 Directed evolution method
US28377101P 2001-04-13 2001-04-13
US28550101P 2001-04-20 2001-04-20

Publications (1)

Publication Number Publication Date
ATE396259T1 true ATE396259T1 (de) 2008-06-15

Family

ID=27255886

Family Applications (2)

Application Number Title Priority Date Filing Date
AT04078077T ATE396259T1 (de) 2000-09-13 2001-09-13 Methode zur zielgerichteten evolution
AT01965462T ATE344320T1 (de) 2000-09-13 2001-09-13 Methode zur zielgerichteten evolution

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT01965462T ATE344320T1 (de) 2000-09-13 2001-09-13 Methode zur zielgerichteten evolution

Country Status (11)

Country Link
US (3) US7514210B2 (de)
EP (4) EP1505151B1 (de)
JP (2) JP5009481B2 (de)
AT (2) ATE396259T1 (de)
AU (2) AU8610301A (de)
CA (2) CA2585083A1 (de)
DE (2) DE60134181D1 (de)
DK (1) DK1317539T3 (de)
ES (2) ES2274901T3 (de)
GB (1) GB0022458D0 (de)
WO (1) WO2002022869A2 (de)

Families Citing this family (111)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2342094B (en) 1997-07-07 2002-10-23 Medical Res Council In vitro sorting method
GB9900298D0 (en) * 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
FR2843404A1 (fr) * 2002-08-07 2004-02-13 Cellectis Methode de determination de l'activite de clivage d'une endonuclease
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
CN101128601B (zh) 2003-01-29 2011-06-08 454生命科学公司 核酸扩增和测序的方法
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307428D0 (en) * 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
JP5183063B2 (ja) 2003-07-05 2013-04-17 ザ ジョンズ ホプキンス ユニバーシティ 遺伝的変異の検出および列挙のための方法ならびに組成物
ES2546945T3 (es) * 2003-11-03 2015-09-30 Medical Research Council Polimerasa
GB0325653D0 (en) * 2003-11-03 2003-12-10 Medical Res Council CST emulsions
EP1691792A4 (de) * 2003-11-24 2008-05-28 Yeda Res & Dev Zusammensetzungen und verfahren für die sortierung in vitro von molekül- und zell-bibliotheken
EP1735458B1 (de) 2004-01-28 2013-07-24 454 Life Sciences Corporation Nukleinsäureamplifikation mit durchlaufemulsion
US7417133B2 (en) * 2004-02-27 2008-08-26 Institut Pasteur Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
CA2615323A1 (en) 2005-06-06 2007-12-21 454 Life Sciences Corporation Paired end sequencing
EP1743938A1 (de) * 2005-07-12 2007-01-17 NascaCell IP GmbH Verfahren zur Oberflächenpräsentation von Polypeptiden
DE102005037351B3 (de) * 2005-08-08 2007-01-11 Geneart Ag Verfahren für die kontinuierliche zielgerichtete Evolution von Proteinen in vitro
DE102005037349A1 (de) * 2005-08-08 2007-02-15 Geneart Ag Verfahren für die kontinuierliche zielgerichtete Evolution von Proteinen in vivo
EP2364774A3 (de) 2006-01-11 2014-06-04 Raindance Technologies, Inc. Mikrofluidische Vorrichtungen Und Verfahren Zur Verwendung Bei Der Bildung Und Kontrolle Von Nanoreaktoren
ATE540750T1 (de) 2006-05-11 2012-01-15 Raindance Technologies Inc Mikrofluidische vorrichtung und verfahren
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
CN101506378A (zh) 2006-06-19 2009-08-12 约翰·霍普金斯大学 在油包水乳液中的微粒上的单分子pcr
EP3536396B1 (de) 2006-08-07 2022-03-30 The President and Fellows of Harvard College Fluorkohlenstoffemulsionsstabilisierende tenside
US8435775B2 (en) 2006-09-06 2013-05-07 Medical Research Council Mutant Pfu DNA polymerase
AU2007296180A1 (en) 2006-09-14 2008-03-20 Dna Polymerase Technology Inc. Use of Taq polymerase mutant enzymes for DNA amplification in the presence of PCR inhibitors
ES2627274T3 (es) 2006-10-23 2017-07-27 Medical Research Council Polimerasa
US7682791B2 (en) * 2006-10-29 2010-03-23 Macevicz Stephen C Method of generating nested sets of double stranded DNA circles
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US8153358B2 (en) 2007-02-23 2012-04-10 New England Biolabs, Inc. Selection and enrichment of proteins using in vitro compartmentalization
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
CN101918595A (zh) 2007-11-27 2010-12-15 人类遗传标记控股有限公司 用于扩增和复制亚硫酸氢盐修饰的核酸的酶
US8748727B2 (en) * 2008-01-18 2014-06-10 Tenksolar, Inc. Flat-plate photovoltaic module
US8933320B2 (en) * 2008-01-18 2015-01-13 Tenksolar, Inc. Redundant electrical architecture for photovoltaic modules
GB0806562D0 (en) 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid
EP2300613A4 (de) * 2008-06-18 2011-11-09 Life Technologies Corp Mutierte und chemisch modifizierte thermisch stabile dna-polymerasen
EP4047367A1 (de) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Verfahren zum nachweis von zielanalyten unter verwendung von tropfenbibliotheken
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
CN102203287B (zh) * 2008-08-26 2017-09-19 弗卢迪格姆公司 增加样本和/或靶通量的测定方法
WO2010062777A2 (en) 2008-11-03 2010-06-03 Kapabiosystems Modified type a dna polymerases
US8528589B2 (en) 2009-03-23 2013-09-10 Raindance Technologies, Inc. Manipulation of microfluidic droplets
GB0904957D0 (en) 2009-03-23 2009-05-06 Univ Erasmus Medical Ct Tumour gene profile
JP5985390B2 (ja) 2009-04-02 2016-09-06 フリューダイム・コーポレイション 標的核酸にバーコードを付加するためのマルチプライマー増幅方法
US8003329B2 (en) * 2009-05-12 2011-08-23 Becton Dickinson & Company Molecular counting by color-coded micelles
WO2010148009A2 (en) * 2009-06-15 2010-12-23 Tenksolar, Inc. Illumination agnostic solar panel
JP2011051943A (ja) * 2009-09-03 2011-03-17 Tosoh Corp タンパク質抽出試薬およびそれを用いたポリメラーゼのスクリーニング方法
WO2011042564A1 (en) 2009-10-09 2011-04-14 Universite De Strasbourg Labelled silica-based nanomaterial with enhanced properties and uses thereof
US10207240B2 (en) * 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
US10837883B2 (en) 2009-12-23 2020-11-17 Bio-Rad Laboratories, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
EP3392349A1 (de) 2010-02-12 2018-10-24 Raindance Technologies, Inc. Digitale analyse von analyten
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
JP2013526273A (ja) 2010-05-14 2013-06-24 ストールハンシェ，ペール キットおよび方法
EP2622103B2 (de) 2010-09-30 2022-11-16 Bio-Rad Laboratories, Inc. Sandwichassays in tröpfchen
WO2012097318A2 (en) 2011-01-14 2012-07-19 Kap Abiosystems Modified dna polymerases for improved amplification
EP3859011A1 (de) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Verfahren zur bildung gemischter tröpfchen
EP2675819B1 (de) 2011-02-18 2020-04-08 Bio-Rad Laboratories, Inc. Zusammensetzungen und verfahren für molekulare etikettierung
WO2012162267A2 (en) 2011-05-20 2012-11-29 Fluidigm Corporation Nucleic acid encoding reactions
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
EP3709018A1 (de) 2011-06-02 2020-09-16 Bio-Rad Laboratories, Inc. Mikrofluidische vorrichtung zum nachweis von komponenten einer chemischen reaktion
EP4249603A3 (de) 2011-06-08 2024-01-03 Life Technologies Corporation Design und entwicklung neuartiger reinigungsmittel zur verwendung in pcr-systemen
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
WO2013013822A1 (en) * 2011-07-28 2013-01-31 Roche Diagnostics Gmbh Dna polymerases with improved activity
GB201113430D0 (en) 2011-08-03 2011-09-21 Fermentas Uab DNA polymerases
CN103857805B (zh) 2011-08-10 2017-07-18 生命技术公司 聚合酶组合物、制备和使用所述聚合酶组合物的方法
US11208636B2 (en) 2011-08-10 2021-12-28 Life Technologies Corporation Polymerase compositions, methods of making and using same
GB201120711D0 (en) 2011-12-01 2012-01-11 Univ Erasmus Medical Ct Method for classifying tumour cells
US20130157259A1 (en) * 2011-12-15 2013-06-20 Samsung Electronics Co., Ltd. Method of amplifying dna from rna in sample and use thereof
WO2013106737A1 (en) 2012-01-13 2013-07-18 Data2Bio Genotyping by next-generation sequencing
EP2809783A2 (de) 2012-02-02 2014-12-10 Invenra, Inc. Hochdurchsatz-screen für biologisch aktive polypeptide
CA2874343C (en) 2012-05-21 2021-11-09 Fluidigm Corporation Single-particle analysis of particle populations
CA3131328C (en) 2012-10-16 2023-10-24 Dna Polymerase Technology, Inc. Inhibition-resistant polymerases
WO2014065760A1 (en) * 2012-10-25 2014-05-01 Agency For Science, Technology And Research Methods for determining resistance against molecules targeting proteins
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9914964B2 (en) 2013-10-25 2018-03-13 Life Technologies Corporation Compounds for use in PCR systems and applications thereof
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
WO2015100277A2 (en) * 2013-12-23 2015-07-02 University Of Rochester Methods and compositions for ribosomal synthesis of macrocyclic peptides
US11193176B2 (en) 2013-12-31 2021-12-07 Bio-Rad Laboratories, Inc. Method for detecting and quantifying latent retroviral RNA species
US9976178B2 (en) * 2014-12-16 2018-05-22 Life Technologies Corporation Polymerase compositions and methods of making and using same
WO2016183294A1 (en) 2015-05-12 2016-11-17 Dna Polymerase Technology, Inc. Mutant polymerases and uses thereof
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
CA3006994A1 (en) 2015-12-16 2017-06-22 Fluidigm Corporation High-level multiplex amplification
JP6934477B2 (ja) 2016-01-19 2021-09-15 ボード オブ リージェンツ ザ ユニヴァーシティ オブ テキサス システム 熱安定逆転写酵素
US10982273B2 (en) 2016-08-09 2021-04-20 Integrated Dna Technologies, Inc. RNase H mutants in an emulsion
US12116570B2 (en) 2016-09-29 2024-10-15 Roche Sequencing Solutions, Inc. Directed evolution of enzymes by plasmid tagging in droplets
US20180135080A1 (en) * 2016-11-15 2018-05-17 Genomic Vision Sa Method for the monitoring of modified nucleases induced-gene editing events by molecular combing
CN108265039B (zh) * 2016-12-30 2020-07-14 天津强微特生物科技有限公司 一种突变TaqDNA聚合酶及其纯化方法
US11299776B2 (en) 2017-05-10 2022-04-12 Board Of Regents, The University Of Texas System Methods and devices related to amplifying nucleic acid at a variety of temperatures
WO2018232282A1 (en) * 2017-06-15 2018-12-20 Board Of Regents, The University Of Texas System Methods and platform for screening and selecting metabolites and their receptors
LT6615B (lt) 2017-09-12 2019-04-25 Vilniaus Universitetas N4-modifikuoti citidino nukleotidai ir jų panaudojimas
US11866785B2 (en) 2017-10-27 2024-01-09 Board Of Regents, The University Of Texas System Tumor specific antibodies and T-cell receptors and methods of identifying the same
EP3740591A4 (de) 2018-01-19 2022-02-23 Bio-Rad Laboratories, Inc. Mutierte dna-polymerasen
LT6742B (lt) * 2018-10-10 2020-07-10 Vilnius University Katalizinio biomolekulinio aktyvumo įrašymas į dnr seką
CN113383086A (zh) * 2019-03-10 2021-09-10 武汉爱博泰克生物科技有限公司 用于在提高的盐浓度或体液中扩增的突变型Taq聚合酶
US11613738B2 (en) * 2019-05-14 2023-03-28 Abclonal Science, Inc. Mutant Taq polymerase resistant to inhibition of amplification in the presence of cyanine dye
CN110218776A (zh) * 2019-06-14 2019-09-10 苏州叠代生物科技有限公司 Pcr油相组合物及其制备方法
WO2021025623A1 (en) * 2019-08-08 2021-02-11 Agency For Science, Technology And Research Method of detecting protein-protein interactions
US12270026B1 (en) 2020-03-05 2025-04-08 Ansa Biotechnologies, Inc. Directed evolution method for template-independent polymerases
CN111621482B (zh) * 2020-06-30 2022-04-29 浙江工业大学 一种草铵膦脱氢酶突变体、基因工程菌及一锅法多酶同步定向进化方法
CN114480327B (zh) * 2020-10-26 2024-01-16 厦门大学 一种Taq DNA聚合酶突变体
EP4301499A4 (de) 2021-03-05 2025-01-22 Enumerix, Inc. Systeme und verfahren zur erzeugung von tröpfchen und zur durchführung digitaler analysen
CN112695019B (zh) * 2021-03-23 2021-06-25 翌圣生物科技（上海）有限公司 逆转录酶突变体及其应用
JPWO2024009873A1 (de) * 2022-07-08 2024-01-11
EP4689163A1 (de) * 2023-04-05 2026-02-11 Primrose Bio, Inc. Verfahren und zusammensetzungen zur protein-engineering
WO2024216275A2 (en) * 2023-04-13 2024-10-17 5Prime Biosciences, Inc. Compositions and methods for upregulation of reverse transcription and reduction of sequence bias in rna sequencing
DE102023001827A1 (de) 2023-04-19 2024-10-24 Horst Wochnowski Verfahren und dazugehörige Vorrichtung zum Züchten von in Gewässern Mikroplastik zersetzenden und abbauenden Mikroorganismenstämme durch einen künstlich herbeigeführten und beschleunigten Evolutions- und Selektionsprozess

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5290479A (en) 1986-12-09 1994-03-01 Phillips Petroleum Company Surfactant system of polyethoxylated compounds and glyceride compounds
GB9107628D0 (en) * 1991-04-10 1991-05-29 Moonbrook Limited Preparation of diagnostic agents
JPH06509473A (ja) * 1991-08-10 1994-10-27 メディカル・リサーチ・カウンシル 細胞個体群の処理
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5910408A (en) * 1995-06-07 1999-06-08 The General Hospital Corporation Catalytic DNA having ligase activity
US6083693A (en) * 1996-06-14 2000-07-04 Curagen Corporation Identification and comparison of protein-protein interactions that occur in populations
WO1998013502A2 (en) * 1996-09-27 1998-04-02 Icos Corporation Method to identify compounds for disrupting protein/protein interactions
WO1998023733A2 (en) * 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
CA2196496A1 (en) * 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
GB2342094B (en) * 1997-07-07 2002-10-23 Medical Res Council In vitro sorting method
WO2000004139A1 (en) * 1998-07-17 2000-01-27 Mirus Corporation Micellar systems
GB9818432D0 (en) * 1998-08-24 1998-10-21 Bioline Limited Thermostable DNA Polymerase
GB9900298D0 (en) * 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
GB0114854D0 (en) * 2001-06-18 2001-08-08 Medical Res Council Selective gene amplification
GB0127564D0 (en) * 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions

Also Published As

Publication number Publication date
US20080166772A1 (en) 2008-07-10
ES2304580T3 (es) 2008-10-16
EP1505151A2 (de) 2005-02-09
EP1505151A3 (de) 2005-05-11
DE60124272D1 (de) 2006-12-14
CA2421963A1 (en) 2002-03-21
EP1317539B1 (de) 2006-11-02
JP5009481B2 (ja) 2012-08-22
AU8610301A (en) 2002-03-26
CA2585083A1 (en) 2002-03-21
DK1317539T3 (da) 2007-03-05
EP1317539A2 (de) 2003-06-11
EP1964932A3 (de) 2009-05-20
DE60124272T2 (de) 2007-05-03
CA2421963C (en) 2013-04-09
EP1964932A2 (de) 2008-09-03
ES2274901T3 (es) 2007-06-01
JP2004508834A (ja) 2004-03-25
ATE344320T1 (de) 2006-11-15
EP1806406A3 (de) 2009-07-29
US20040005594A1 (en) 2004-01-08
WO2002022869A3 (en) 2002-10-17
US20090246853A1 (en) 2009-10-01
JP2007190027A (ja) 2007-08-02
WO2002022869A2 (en) 2002-03-21
GB0022458D0 (en) 2000-11-01
EP1806406A2 (de) 2007-07-11
US7514210B2 (en) 2009-04-07
AU2001286103B2 (en) 2006-08-10
DE60134181D1 (de) 2008-07-03
EP1505151B1 (de) 2008-05-21

Similar Documents

Publication Publication Date Title
DE60134181D1 (de) Methode zur zielgerichteten Evolution
ATE374815T1 (de) Methode zur erhöhung der konzentration von nucleinsäuremolekülen
DE60222307D1 (de) Methode zur Anreicherung von Nukleinsäuremolekülen
EA200300284A1 (ru) Способ амплификации нуклеиновых кислот
KR910008135A (ko) 단백질 분해 효소를 사용하지 않는 핵산의 추출 및 pcr 증폭방법
ATE324464T1 (de) Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren
PT1179600E (pt) Monitorizacao da hibridacao durante a rcp
ATE148925T1 (de) Methode zur amplification von ziel-gerichteten segmenten von nukleinsäure durch die ineinandergeschachtelte polymerase-kettenreaktion
NO941064D0 (no) Selektiv restriksjonsfragmentamplifikasjon: en generell metode for DNA fingerprinting
DE69936201D1 (de) Enzymstabilisierung durch kationische, oberflächenaktive substanzen
DE60039037D1 (de) Methode zur direkten sequenzierung von nukleinsäuren
DE60235383D1 (de) Einzelnukleotidamplifikation und -nachweis mit polymerase
DE69321112D1 (de) Elektrochemische Behandlung von Nukleinsäure - enthaltenden Lösungen
DK1026261T3 (da) Forögelse af specificiteten af nukleinsyreamplifikation med bærernukleinsyre
DE69427876D1 (de) Methode zur detektion einer gezielten nukleinsäure
DE50102587D1 (de) Kontaktierungsvorrichtung
ATE403663T1 (de) Nukleinsäureamplifikation mit duplexzwischenprodukten
ES2109983T3 (es) Proceso para controlar la contaminacion de reacciones de amplificacion de acidos nucleicos.
Milton et al. Site-specific disulfide bridges in oligodeoxyribonucleotide duplexes containing 6-mercaptopurine and 4-thiothymine bases
EP1170383A3 (de) Oligonukleotide und Verfahren zur Bestimmung von SRSV RNA
DE69833864D1 (de) Methoden zur dns vervielfältigung und material dafür
DE60127244D1 (de) Spezifische multiplex-analyse von nukleinsäuren
ES2188357B1 (es) Metodo para la amplificacion mediante transcripcion en reverso acoplada a una reaccion de nested o semi-nested pcr en un solo paso, de un arn diana, y sus aplicaciones en la identificacion de enterovirus y en la discriminacion entre poliovirus y enterovirus no-polio.
ES2193861A1 (es) Metodo para la identificacion molecular de fusarium oxysporum f. sp. ciceris y sus razas patogenicas 0,5 y 6 mediante pcr especifica.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties