DE60124272D1 - Methode zur zielgerichteten evolution - Google Patents

Methode zur zielgerichteten evolution

Info

Publication number
DE60124272D1
DE60124272D1 DE60124272T DE60124272T DE60124272D1 DE 60124272 D1 DE60124272 D1 DE 60124272D1 DE 60124272 T DE60124272 T DE 60124272T DE 60124272 T DE60124272 T DE 60124272T DE 60124272 D1 DE60124272 D1 DE 60124272D1
Authority
DE
Germany
Prior art keywords
nucleic acid
microcompartments
concentration
increasing
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60124272T
Other languages
English (en)
Other versions
DE60124272T2 (de
Inventor
Philipp Holliger
Farid Ghadessy
Jennifer Ong
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medical Research Council
Original Assignee
Medical Research Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medical Research Council filed Critical Medical Research Council
Application granted granted Critical
Publication of DE60124272D1 publication Critical patent/DE60124272D1/de
Publication of DE60124272T2 publication Critical patent/DE60124272T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Ecology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Electrotherapy Devices (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Soil Working Implements (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
DE60124272T 2000-09-13 2001-09-13 Methode zur zielgerichteten evolution Expired - Lifetime DE60124272T2 (de)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
GB0022458 2000-09-13
GBGB0022458.4A GB0022458D0 (en) 2000-09-13 2000-09-13 Directed evolution method
US28377101P 2001-04-13 2001-04-13
US283771P 2001-04-13
US28550101P 2001-04-20 2001-04-20
US285501P 2001-04-20
PCT/GB2001/004108 WO2002022869A2 (en) 2000-09-13 2001-09-13 Directed evolution method

Publications (2)

Publication Number Publication Date
DE60124272D1 true DE60124272D1 (de) 2006-12-14
DE60124272T2 DE60124272T2 (de) 2007-05-03

Family

ID=27255886

Family Applications (2)

Application Number Title Priority Date Filing Date
DE60124272T Expired - Lifetime DE60124272T2 (de) 2000-09-13 2001-09-13 Methode zur zielgerichteten evolution
DE60134181T Expired - Lifetime DE60134181D1 (de) 2000-09-13 2001-09-13 Methode zur zielgerichteten Evolution

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE60134181T Expired - Lifetime DE60134181D1 (de) 2000-09-13 2001-09-13 Methode zur zielgerichteten Evolution

Country Status (11)

Country Link
US (3) US7514210B2 (de)
EP (4) EP1317539B1 (de)
JP (2) JP5009481B2 (de)
AT (2) ATE344320T1 (de)
AU (2) AU8610301A (de)
CA (2) CA2421963C (de)
DE (2) DE60124272T2 (de)
DK (1) DK1317539T3 (de)
ES (2) ES2274901T3 (de)
GB (1) GB0022458D0 (de)
WO (1) WO2002022869A2 (de)

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Also Published As

Publication number Publication date
US7514210B2 (en) 2009-04-07
US20080166772A1 (en) 2008-07-10
EP1964932A2 (de) 2008-09-03
ES2274901T3 (es) 2007-06-01
ES2304580T3 (es) 2008-10-16
ATE396259T1 (de) 2008-06-15
CA2585083A1 (en) 2002-03-21
EP1806406A2 (de) 2007-07-11
EP1505151B1 (de) 2008-05-21
WO2002022869A3 (en) 2002-10-17
US20040005594A1 (en) 2004-01-08
EP1505151A2 (de) 2005-02-09
DE60124272T2 (de) 2007-05-03
ATE344320T1 (de) 2006-11-15
WO2002022869A2 (en) 2002-03-21
EP1505151A3 (de) 2005-05-11
CA2421963C (en) 2013-04-09
US20090246853A1 (en) 2009-10-01
JP2007190027A (ja) 2007-08-02
EP1317539B1 (de) 2006-11-02
DE60134181D1 (de) 2008-07-03
CA2421963A1 (en) 2002-03-21
DK1317539T3 (da) 2007-03-05
EP1964932A3 (de) 2009-05-20
JP5009481B2 (ja) 2012-08-22
JP2004508834A (ja) 2004-03-25
EP1317539A2 (de) 2003-06-11
EP1806406A3 (de) 2009-07-29
AU8610301A (en) 2002-03-26
GB0022458D0 (en) 2000-11-01
AU2001286103B2 (en) 2006-08-10

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