KR910008135A - 단백질 분해 효소를 사용하지 않는 핵산의 추출 및 pcr 증폭방법 - Google Patents

단백질 분해 효소를 사용하지 않는 핵산의 추출 및 pcr 증폭방법 Download PDF

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KR910008135A
KR910008135A KR1019900016541A KR900016541A KR910008135A KR 910008135 A KR910008135 A KR 910008135A KR 1019900016541 A KR1019900016541 A KR 1019900016541A KR 900016541 A KR900016541 A KR 900016541A KR 910008135 A KR910008135 A KR 910008135A
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nucleic acids
nucleic acid
cells
composition
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조세프 쿠민스 토마스
데이비스 에케제 토비아스
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제이. 제프리 흘리
이스트만 코닥 캄파니
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Abstract

내용 없음

Description

단백질 분해 효소를 사용하지 않는 핵산의 추출 및 PCR 증폭방법.
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (10)

  1. A. 하나 이상의 핵산을 함유하는 세포 또는 비리온의 샘플을, 단백질 분해효소가 없는 상태에서 (1)조성물을 pH4내지 10으로 유지시키는 유기 완충제. (2)DNA 중합 효소 활성을 위한 보조 인자의 촉매량의 공급원. (3)안정화제. (4)세포 또는 비리온으로부터 핵산을 방출하기에 충분한 양으로 존해하는 적어도 하나의 상존성 비이온성 계면활성제를 포함하는 분해 조성물과 혼합하고 B. 생성된 혼합물을 물의 비점 또는 그 부근에서 5내지 15분동안 가열하며, C. 가열된 혼합물로부터 핵산을 회수하는 것을 포함하는 세포 또는 비리온으로부터 핵산의 신속한 추출방법.
  2. I.A. 하나의 이상의 핵산을 함유하는 세포 또는 비리온의 샘플을, 단백질 분해 효소가 없는 상태에서, (1)조성물을 pH4 내지 10으로 유지시키는 유기 완충제. (2)DNA 중합 효소 활성을 위한 보조 인자의 촉매량의 공급원. (3)안정화제. (4)세포 또는 비리온으로부터 핵산을 방출하기에 충분한 양으로 존해하는 적어도 하나이상의 상존성 비이온성 계면활성제를 포함하는 분해 조성물과 혼합하고 B. 생성된 혼합물을 물의 비점 또는 그 부근에서 5내지 15분동안 가열하며, C. 가열된 혼합물로부터 핵산을 회수하는 것을 포함하는 추출 공정 및 Ⅱ. 중합효소 연쇄반응을 이용하여 회수된 핵산을 증폭시키는 것을 포함하는 중합효소 연쇄반응을 이용한 핵산의 증폭 방법.
  3. 제1항 또는 제2항에 있어서, 조성물이 추가로 1 내지 150밀리몰 양의 염을 포함하는 방법.
  4. 제1항 내지 제3항중 어느 한 항에 있어서, DNA중합효소 보조인자가 막네슘 또는 망간염으로서 공급되는 방법.
  5. 제1항 내지 제4항중 어느 한 항에 있어서, 안정화제가 제라틴 또는 그의 유도체인 방법.
  6. 제1항 내지 제5항중 어느 한 항에 있어서, 조성물이 하나는 폴리옥시에틸렌 소르비탄 유도체이고 다른 하나는 폴리옥시에틸렌에테르인 두가지의 비이온성 계면활성제를 포함하는 방법.
  7. 제1항 내지 제6항중 어느 한 항에 있어서, 비이온성 계면활성제가 총 조성물의 중량을 기준으로 0.1 내지 5%로 존재하는 방법.
  8. 제1항 내지 제7항중 어느 한 항에 있어서, 혼합물을 8내지 12분동안 가열시키는 방법.
  9. 제1항 내지 제8항중 어느 한 항에 있어서, 바이러스 DNA의 추출 또는 증폭을 위한 방법.
  10. 제1항 내지 제9항중 어느 한 항에 있어서, 샘플이 전혈 또는그의 성분인 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019900016541A 1989-10-18 1990-10-17 단백질 분해 효소를 사용하지 않는 핵산의 추출 및 pcr 증폭방법 KR910008135A (ko)

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US423071 1989-10-18
US07/423,071 US5231015A (en) 1989-10-18 1989-10-18 Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme

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EP (1) EP0428197B1 (ko)
JP (1) JP2533969B2 (ko)
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AT (1) ATE147108T1 (ko)
CA (1) CA2025845A1 (ko)
DE (1) DE69029563T2 (ko)
FI (1) FI905145A0 (ko)
HK (1) HK113997A (ko)
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SG (1) SG91238A1 (ko)

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