ES2162875T3 - Metodo para la supresion de la inhibicion de reacciones inducidas por enzimas mediante detergentes ionicos. - Google Patents

Metodo para la supresion de la inhibicion de reacciones inducidas por enzimas mediante detergentes ionicos.

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Publication number
ES2162875T3
ES2162875T3 ES95103520T ES95103520T ES2162875T3 ES 2162875 T3 ES2162875 T3 ES 2162875T3 ES 95103520 T ES95103520 T ES 95103520T ES 95103520 T ES95103520 T ES 95103520T ES 2162875 T3 ES2162875 T3 ES 2162875T3
Authority
ES
Spain
Prior art keywords
ionic detergent
inhibition
enzymes
suppression
reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES95103520T
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English (en)
Inventor
Daniel Louis Kacian
Diane Lisa Mcallister
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gen Probe Inc
Original Assignee
Gen Probe Inc
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Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22789688&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2162875(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Application granted granted Critical
Publication of ES2162875T3 publication Critical patent/ES2162875T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)

Abstract

UNOS METODOS Y EQUIPOS PARA UTILIZAR DETERGENTES NO IONICOS PARA SUPRIMIR LA INHIBICION ENZIMATICA EN UNA DISOLUCION DE REACCION DEBIDO A LA PRESENCIA DE DETERGENTE IONICO DE INHIBICION. ANTES DE LA REACCION, LA MEZCLA DE REACCION SE DA CON UNA CANTIDAD EFECTIVA DE DETERGENTE NO IONICO Y SE AGITA. LA ENZIMA SE AÑADE ENTONCES, Y LA REACCION ENZIMATICA ES LA PERMITIDA PARA EL PROCESO. TAMBIEN SE DESCRIBEN UNAS EXPRESIONES PREFERIDAS DE LA PRESENTE INVENCION, Y LOS EQUIPOS PARA AMPLIFICACION DE ACIDOS NUCLEICOS DE UNA MUESTRA BIOLOGICA EN PRESENCIA DE UN DETERGENTE IONICO.
ES95103520T 1994-03-10 1995-03-10 Metodo para la supresion de la inhibicion de reacciones inducidas por enzimas mediante detergentes ionicos. Expired - Lifetime ES2162875T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US21213194A 1994-03-10 1994-03-10

Publications (1)

Publication Number Publication Date
ES2162875T3 true ES2162875T3 (es) 2002-01-16

Family

ID=22789688

Family Applications (1)

Application Number Title Priority Date Filing Date
ES95103520T Expired - Lifetime ES2162875T3 (es) 1994-03-10 1995-03-10 Metodo para la supresion de la inhibicion de reacciones inducidas por enzimas mediante detergentes ionicos.

Country Status (11)

Country Link
US (3) US5766890A (es)
EP (1) EP0671473B1 (es)
JP (1) JP3062250B2 (es)
KR (1) KR100238833B1 (es)
AT (1) ATE206465T1 (es)
AU (1) AU699232B2 (es)
CA (1) CA2184270C (es)
DE (1) DE69522978T2 (es)
DK (1) DK0671473T3 (es)
ES (1) ES2162875T3 (es)
WO (1) WO1995024499A1 (es)

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US6436677B1 (en) * 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
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ATE481507T1 (de) * 2004-01-23 2010-10-15 Bio Merieux Inc Primer- und sondenkonstruktion zur effizienten amplifikation und detektion des 3'- nichttranslatierten bereichs von hcv
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FR2878254B1 (fr) * 2004-11-22 2010-08-20 Bio Rad Pasteur Composition pour l'amplification d'acides nucleiques
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US7972828B2 (en) * 2006-12-19 2011-07-05 Sigma-Aldrich Co. Stabilized compositions of thermostable DNA polymerase and anionic or zwitterionic detergent
US9181554B2 (en) * 2007-04-05 2015-11-10 Australian Centre for Plant Functional Genomics Pty, Ltd Methods for detecting a target nucleotide sequence in a sample utilising a nuclease-aptamer complex
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JP2010528669A (ja) * 2007-06-13 2010-08-26 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション ポリメラーゼ安定化
JP5546109B2 (ja) * 2008-05-27 2014-07-09 富士フイルム株式会社 核酸の塩基配列の識別方法
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Also Published As

Publication number Publication date
EP0671473A1 (en) 1995-09-13
US5871975A (en) 1999-02-16
WO1995024499A1 (en) 1995-09-14
KR970701787A (ko) 1997-04-12
DE69522978D1 (de) 2001-11-08
AU699232B2 (en) 1998-11-26
DE69522978T2 (de) 2002-04-04
CA2184270A1 (en) 1995-09-14
JP3062250B2 (ja) 2000-07-10
EP0671473B1 (en) 2001-10-04
DK0671473T3 (da) 2001-10-29
JPH09510099A (ja) 1997-10-14
US5766890A (en) 1998-06-16
AU2097895A (en) 1995-09-25
US5846701A (en) 1998-12-08
KR100238833B1 (ko) 2000-03-02
CA2184270C (en) 1999-08-17
ATE206465T1 (de) 2001-10-15

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