ATE206465T1 - Verfahren zur unterdrückung der hemmung von enzym-vermittelten reaktionen durch ionische detergentien - Google Patents
Verfahren zur unterdrückung der hemmung von enzym-vermittelten reaktionen durch ionische detergentienInfo
- Publication number
- ATE206465T1 ATE206465T1 AT95103520T AT95103520T ATE206465T1 AT E206465 T1 ATE206465 T1 AT E206465T1 AT 95103520 T AT95103520 T AT 95103520T AT 95103520 T AT95103520 T AT 95103520T AT E206465 T1 ATE206465 T1 AT E206465T1
- Authority
- AT
- Austria
- Prior art keywords
- enzyme
- ionic detergent
- inhibition
- suppressing
- reaction
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Detergent Compositions (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US21213194A | 1994-03-10 | 1994-03-10 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE206465T1 true ATE206465T1 (de) | 2001-10-15 |
Family
ID=22789688
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT95103520T ATE206465T1 (de) | 1994-03-10 | 1995-03-10 | Verfahren zur unterdrückung der hemmung von enzym-vermittelten reaktionen durch ionische detergentien |
Country Status (11)
Country | Link |
---|---|
US (3) | US5846701A (de) |
EP (1) | EP0671473B1 (de) |
JP (1) | JP3062250B2 (de) |
KR (1) | KR100238833B1 (de) |
AT (1) | ATE206465T1 (de) |
AU (1) | AU699232B2 (de) |
CA (1) | CA2184270C (de) |
DE (1) | DE69522978T2 (de) |
DK (1) | DK0671473T3 (de) |
ES (1) | ES2162875T3 (de) |
WO (1) | WO1995024499A1 (de) |
Families Citing this family (42)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5514551A (en) * | 1994-10-14 | 1996-05-07 | Gen-Probe Incorporated | Compositions for the detection of Chlamydia trachomatis |
JP2000500342A (ja) | 1995-11-15 | 2000-01-18 | ジェン−プローブ・インコーポレーテッド | ヒトパピローマウイルス核酸に相補的な核酸プローブおよび関連する方法およびキット |
US5800997A (en) * | 1996-11-01 | 1998-09-01 | Novartis Finance Corporation | Detection of maize fungal pathogens using the polymerase chain reaction |
IL124275A (en) * | 1997-05-02 | 2002-03-10 | Bio Merieux Vitek Inc | A method to produce sequences of nucleic acids |
US6558901B1 (en) | 1997-05-02 | 2003-05-06 | Biomerieux Vitek | Nucleic acid assays |
EP0989192A3 (de) * | 1998-09-21 | 2004-02-25 | Shimadzu Corporation | Verfahren zur Nucleinsäuresynthese |
US6242188B1 (en) | 1999-07-30 | 2001-06-05 | Applied Gene Technologies, Inc. | Sample processing to release nucleic acids for direct detection |
GB9922971D0 (en) * | 1999-09-29 | 1999-12-01 | Secr Defence | Reaction system |
US6632557B1 (en) * | 1999-10-26 | 2003-10-14 | The Gillette Company | Cathodes for metal air electrochemical cells |
US20020009799A1 (en) * | 1999-12-10 | 2002-01-24 | Goffe Randal A. | Isolation and purification of nucleic acids |
US6436677B1 (en) * | 2000-03-02 | 2002-08-20 | Promega Corporation | Method of reverse transcription |
WO2002014548A1 (en) * | 2000-08-10 | 2002-02-21 | Applied Gene Technologies, Inc. | Compositions and methods for nucleic acids sample processing and amplification |
GB0110476D0 (en) * | 2001-04-30 | 2001-06-20 | Secr Defence | Reagent delivery system |
US20030170617A1 (en) | 2002-01-28 | 2003-09-11 | Pasloske Brittan L. | Crude biological derivatives competent for nucleic acid detection |
WO2004042003A2 (en) | 2002-11-01 | 2004-05-21 | Promega Corporation | Cell lysis compositions, methods of use, apparatus, and kit |
WO2005070027A2 (en) * | 2004-01-23 | 2005-08-04 | Biomerieux, Inc. | A novel nucleotide mixture for improved nucleic acid amplification performance |
US20050277121A1 (en) * | 2004-06-11 | 2005-12-15 | Ambion, Inc. | Crude biological derivatives competent for nucleic acid detection |
WO2006047482A2 (en) * | 2004-10-22 | 2006-05-04 | Redpath Integrated Pathology, Inc. | Method for determining the diagnosis, malignant potential, and biologic behavior of pancreatic cysts using cyst aspirate |
FR2878254B1 (fr) * | 2004-11-22 | 2010-08-20 | Bio Rad Pasteur | Composition pour l'amplification d'acides nucleiques |
EP1930730B1 (de) | 2005-03-10 | 2019-08-14 | Gen-Probe Incorporated | Systeme und Verfahren zur Durchführung von Tests zum Nachweis oder zur Quantifizierung von Analyten |
CA2967430C (en) | 2005-03-10 | 2018-05-08 | Gen-Probe Incorporated | Systems and methods to perform assays for detecting or quantifying analytes within samples |
US7972828B2 (en) | 2006-12-19 | 2011-07-05 | Sigma-Aldrich Co. | Stabilized compositions of thermostable DNA polymerase and anionic or zwitterionic detergent |
CN101809164B (zh) * | 2007-04-05 | 2014-08-20 | 澳大利亚植物功能基因组学中心控股有限公司 | 利用核酸酶-适配体复合物检测样品中靶核苷酸序列的方法 |
US7964350B1 (en) | 2007-05-18 | 2011-06-21 | Applied Biosystems, Llc | Sample preparation for in situ nucleic acid analysis |
US20100159528A1 (en) * | 2007-06-13 | 2010-06-24 | Ge Healthcare Bio-Science Corp. | Polymerase stabilization |
JP5546109B2 (ja) * | 2008-05-27 | 2014-07-09 | 富士フイルム株式会社 | 核酸の塩基配列の識別方法 |
US8097412B2 (en) * | 2008-07-12 | 2012-01-17 | Biodiagnostics, Inc. | DNA-based test for detection of annual and intermediate ryegrass |
WO2010071833A1 (en) | 2008-12-19 | 2010-06-24 | Life Technologies Corporation | Proteinase k inhibitors, methods and compositions therefor |
CN103403533B (zh) | 2011-02-24 | 2017-02-15 | 简.探针公司 | 用于分辨光信号检测器中不同调制频率的光信号的系统和方法 |
DK2718260T3 (en) | 2011-06-08 | 2018-11-19 | Life Technologies Corp | DESIGN AND DEVELOPMENT OF NEW DETERGENTS FOR USE IN PCR SYSTEMS |
WO2012170907A2 (en) | 2011-06-08 | 2012-12-13 | Life Technologies Corporation | Polymerization of nucleic acids using proteins having low isoelectric points |
WO2013050881A2 (en) | 2011-10-05 | 2013-04-11 | Spartan Bioscience Inc. | Direct nucleic acid analysis |
AU2013202804A1 (en) | 2012-06-14 | 2014-01-16 | Gen-Probe Incorporated | Use of a fluorescent material to detect failure or deteriorated performance of a fluorometer |
US9341628B2 (en) | 2012-11-30 | 2016-05-17 | Interpace Diagnostics Corporation | Methods for measuring carcinoembryonic antigen |
US9914964B2 (en) | 2013-10-25 | 2018-03-13 | Life Technologies Corporation | Compounds for use in PCR systems and applications thereof |
BR112017013277B1 (pt) * | 2014-12-23 | 2023-02-23 | 3M Innovative Properties Company | Composição aquosa e método de amplificação isotérmicade ácido nucleico |
US10391498B2 (en) | 2015-12-11 | 2019-08-27 | Spartan Bioscience Inc. | Systems and methods for nucleic acid amplification |
CN108369180B (zh) | 2015-12-31 | 2022-12-16 | 简·探针公司 | 用于分析样品和监测光信号检测器的性能的系统和方法 |
LT3402880T (lt) | 2016-01-15 | 2024-03-12 | Thermo Fisher Scientific Baltics Uab | Termofiliniai dnr polimerazės mutantai |
US11299777B2 (en) | 2016-04-04 | 2022-04-12 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
US9617587B1 (en) | 2016-04-04 | 2017-04-11 | Nat Diagnostics, Inc. | Isothermal amplification components and processes |
EP3645710A1 (de) | 2017-06-26 | 2020-05-06 | Thermo Fisher Scientific Baltics Uab | Thermophile dna-polymerase-mutanten |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8722252D0 (en) * | 1987-09-22 | 1987-10-28 | Brewing Res Found | Atp extraction |
US5358690A (en) * | 1989-01-10 | 1994-10-25 | Lamina, Ltd. | Environmental sample collection and membrane testing device |
US5231015A (en) * | 1989-10-18 | 1993-07-27 | Eastman Kodak Company | Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme |
US5284940A (en) * | 1990-11-14 | 1994-02-08 | Hri Research, Inc. | Preparation for nucleic acid samples |
EP0488243A1 (de) * | 1990-11-30 | 1992-06-03 | Sanwa Kagaku Kenkyusho Co., Ltd. | Verfahren zur Extraktion des viralen Genoms von einer von einem lebenden Körper abgeleiteten Probe, die mit dem Virus infiziert ist sowie Nachweis des Genoms |
US5175094A (en) * | 1991-08-01 | 1992-12-29 | Becton Dickinson And Company | Increased expression of HBcAg |
AU668746B2 (en) * | 1992-06-12 | 1996-05-16 | Gen-Probe Incorporated | Preparation of nucleic acid from blood |
WO1994026867A1 (en) * | 1993-05-06 | 1994-11-24 | Abbott Laboratories | Direct lysis buffer and the detection of hiv-1 plasma viremia |
US5538870A (en) * | 1994-09-21 | 1996-07-23 | Boehringer Mannheim Corporation | Method for preparing nucleic acids for analysis and kits useful therefor |
-
1995
- 1995-03-07 CA CA002184270A patent/CA2184270C/en not_active Expired - Fee Related
- 1995-03-07 WO PCT/US1995/002865 patent/WO1995024499A1/en active Application Filing
- 1995-03-07 JP JP7523607A patent/JP3062250B2/ja not_active Expired - Fee Related
- 1995-03-07 AU AU20978/95A patent/AU699232B2/en not_active Ceased
- 1995-03-07 KR KR1019960704966A patent/KR100238833B1/ko not_active IP Right Cessation
- 1995-03-10 DK DK95103520T patent/DK0671473T3/da active
- 1995-03-10 EP EP95103520A patent/EP0671473B1/de not_active Expired - Lifetime
- 1995-03-10 AT AT95103520T patent/ATE206465T1/de not_active IP Right Cessation
- 1995-03-10 DE DE69522978T patent/DE69522978T2/de not_active Expired - Lifetime
- 1995-03-10 ES ES95103520T patent/ES2162875T3/es not_active Expired - Lifetime
-
1997
- 1997-01-06 US US08/779,342 patent/US5846701A/en not_active Expired - Lifetime
- 1997-01-06 US US08/779,341 patent/US5766890A/en not_active Expired - Fee Related
- 1997-03-25 US US08/823,568 patent/US5871975A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
AU2097895A (en) | 1995-09-25 |
US5766890A (en) | 1998-06-16 |
DE69522978D1 (de) | 2001-11-08 |
WO1995024499A1 (en) | 1995-09-14 |
DE69522978T2 (de) | 2002-04-04 |
DK0671473T3 (da) | 2001-10-29 |
EP0671473B1 (de) | 2001-10-04 |
US5871975A (en) | 1999-02-16 |
KR970701787A (ko) | 1997-04-12 |
AU699232B2 (en) | 1998-11-26 |
EP0671473A1 (de) | 1995-09-13 |
US5846701A (en) | 1998-12-08 |
JP3062250B2 (ja) | 2000-07-10 |
ES2162875T3 (es) | 2002-01-16 |
JPH09510099A (ja) | 1997-10-14 |
CA2184270C (en) | 1999-08-17 |
CA2184270A1 (en) | 1995-09-14 |
KR100238833B1 (ko) | 2000-03-02 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
UEP | Publication of translation of european patent specification | ||
REN | Ceased due to non-payment of the annual fee |