DE69522978T2 - Verfahren zur Unterdrückung der Hemmung von Enzym-vermittelten Reaktionen durch ionische Detergentien - Google Patents

Verfahren zur Unterdrückung der Hemmung von Enzym-vermittelten Reaktionen durch ionische Detergentien

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Publication number
DE69522978T2
DE69522978T2 DE69522978T DE69522978T DE69522978T2 DE 69522978 T2 DE69522978 T2 DE 69522978T2 DE 69522978 T DE69522978 T DE 69522978T DE 69522978 T DE69522978 T DE 69522978T DE 69522978 T2 DE69522978 T2 DE 69522978T2
Authority
DE
Germany
Prior art keywords
enzyme
ionic detergent
inhibition
suppressing
reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69522978T
Other languages
English (en)
Other versions
DE69522978D1 (de
Inventor
Daniel Louis Kacian
Diane Lisa Mcallister
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gen Probe Inc
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22789688&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69522978(T2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Publication of DE69522978D1 publication Critical patent/DE69522978D1/de
Application granted granted Critical
Publication of DE69522978T2 publication Critical patent/DE69522978T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
DE69522978T 1994-03-10 1995-03-10 Verfahren zur Unterdrückung der Hemmung von Enzym-vermittelten Reaktionen durch ionische Detergentien Expired - Lifetime DE69522978T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US21213194A 1994-03-10 1994-03-10

Publications (2)

Publication Number Publication Date
DE69522978D1 DE69522978D1 (de) 2001-11-08
DE69522978T2 true DE69522978T2 (de) 2002-04-04

Family

ID=22789688

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69522978T Expired - Lifetime DE69522978T2 (de) 1994-03-10 1995-03-10 Verfahren zur Unterdrückung der Hemmung von Enzym-vermittelten Reaktionen durch ionische Detergentien

Country Status (11)

Country Link
US (3) US5846701A (de)
EP (1) EP0671473B1 (de)
JP (1) JP3062250B2 (de)
KR (1) KR100238833B1 (de)
AT (1) ATE206465T1 (de)
AU (1) AU699232B2 (de)
CA (1) CA2184270C (de)
DE (1) DE69522978T2 (de)
DK (1) DK0671473T3 (de)
ES (1) ES2162875T3 (de)
WO (1) WO1995024499A1 (de)

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US5800997A (en) * 1996-11-01 1998-09-01 Novartis Finance Corporation Detection of maize fungal pathogens using the polymerase chain reaction
US6558901B1 (en) * 1997-05-02 2003-05-06 Biomerieux Vitek Nucleic acid assays
IL124275A (en) * 1997-05-02 2002-03-10 Bio Merieux Vitek Inc A method to produce sequences of nucleic acids
EP0989192A3 (de) * 1998-09-21 2004-02-25 Shimadzu Corporation Verfahren zur Nucleinsäuresynthese
US6242188B1 (en) 1999-07-30 2001-06-05 Applied Gene Technologies, Inc. Sample processing to release nucleic acids for direct detection
GB9922971D0 (en) * 1999-09-29 1999-12-01 Secr Defence Reaction system
US6632557B1 (en) * 1999-10-26 2003-10-14 The Gillette Company Cathodes for metal air electrochemical cells
AU4312201A (en) * 1999-12-10 2001-06-18 Genespan Corporation Isolation and purification of nucleic acids
US6436677B1 (en) * 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
WO2002014548A1 (en) * 2000-08-10 2002-02-21 Applied Gene Technologies, Inc. Compositions and methods for nucleic acids sample processing and amplification
GB0110476D0 (en) 2001-04-30 2001-06-20 Secr Defence Reagent delivery system
EP1476573B1 (de) * 2002-01-28 2010-01-06 Ambion, Inc. Für den nachweis von nukleinsäuren kompetente biologische rohderivate
WO2004042003A2 (en) 2002-11-01 2004-05-21 Promega Corporation Cell lysis compositions, methods of use, apparatus, and kit
US7364854B2 (en) * 2004-01-23 2008-04-29 Biomerieux, Inc Nucleotide mixture for improved nucleic acid amplification performance
US20050277121A1 (en) * 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
CA2584723A1 (en) 2004-10-22 2006-05-04 Redpath Integrated Pathology, Inc. Enhanced amplifiability of minute fixative-treated tissue samples, minute stained cytology samples, and other minute sources of dna
FR2878254B1 (fr) * 2004-11-22 2010-08-20 Bio Rad Pasteur Composition pour l'amplification d'acides nucleiques
EP1861721B1 (de) 2005-03-10 2017-05-03 Gen-Probe Incorporated Systeme und verfahren zur durchführung von prüfungen zum nachweis oder zur quantifizierung von analyten in proben
EP1909108B1 (de) 2005-03-10 2019-05-29 Gen-Probe Incorporated Systeme und Verfahren zur Durchführung von Tests zum Nachweis oder zur Quantifizierung von Analyten
US7972828B2 (en) 2006-12-19 2011-07-05 Sigma-Aldrich Co. Stabilized compositions of thermostable DNA polymerase and anionic or zwitterionic detergent
WO2008122088A1 (en) * 2007-04-05 2008-10-16 Lifeprint Australia Pty Ltd Methods for detecting a target nucleotide sequence in a sample utilising a nuclease-aptamer complex
US7964350B1 (en) 2007-05-18 2011-06-21 Applied Biosystems, Llc Sample preparation for in situ nucleic acid analysis
US20100159528A1 (en) * 2007-06-13 2010-06-24 Ge Healthcare Bio-Science Corp. Polymerase stabilization
JP5546109B2 (ja) * 2008-05-27 2014-07-09 富士フイルム株式会社 核酸の塩基配列の識別方法
US8097412B2 (en) * 2008-07-12 2012-01-17 Biodiagnostics, Inc. DNA-based test for detection of annual and intermediate ryegrass
WO2010071833A1 (en) 2008-12-19 2010-06-24 Life Technologies Corporation Proteinase k inhibitors, methods and compositions therefor
AU2012222178B2 (en) 2011-02-24 2014-12-18 Gen-Probe Incorporated Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
CN106518696B (zh) 2011-06-08 2019-06-14 生命技术公司 用于pcr系统的新型去污剂的设计和开发
EP2764112B1 (de) * 2011-10-05 2018-04-18 Spartan Bioscience Inc. Direkte nukleinsäureanalyse
AU2013202804A1 (en) 2012-06-14 2014-01-16 Gen-Probe Incorporated Use of a fluorescent material to detect failure or deteriorated performance of a fluorometer
US9341628B2 (en) 2012-11-30 2016-05-17 Interpace Diagnostics Corporation Methods for measuring carcinoembryonic antigen
JP6742238B2 (ja) 2013-10-25 2020-08-19 ライフ テクノロジーズ コーポレーション Ｐｃｒシステムにおいて使用するための新規化合物及びその用途
BR112017013277B1 (pt) * 2014-12-23 2023-02-23 3M Innovative Properties Company Composição aquosa e método de amplificação isotérmicade ácido nucleico
US10391498B2 (en) 2015-12-11 2019-08-27 Spartan Bioscience Inc. Systems and methods for nucleic acid amplification
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Also Published As

Publication number Publication date
EP0671473B1 (de) 2001-10-04
CA2184270A1 (en) 1995-09-14
US5846701A (en) 1998-12-08
CA2184270C (en) 1999-08-17
WO1995024499A1 (en) 1995-09-14
DE69522978D1 (de) 2001-11-08
US5871975A (en) 1999-02-16
KR970701787A (ko) 1997-04-12
KR100238833B1 (ko) 2000-03-02
ATE206465T1 (de) 2001-10-15
AU2097895A (en) 1995-09-25
EP0671473A1 (de) 1995-09-13
JPH09510099A (ja) 1997-10-14
AU699232B2 (en) 1998-11-26
DK0671473T3 (da) 2001-10-29
JP3062250B2 (ja) 2000-07-10
ES2162875T3 (es) 2002-01-16
US5766890A (en) 1998-06-16

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