EP0698082A4 - Ein puffer zur direkten lyse und die feststellung der hiv-1 plasma virämie - Google Patents

Ein puffer zur direkten lyse und die feststellung der hiv-1 plasma virämie

Info

Publication number
EP0698082A4
EP0698082A4 EP94915927A EP94915927A EP0698082A4 EP 0698082 A4 EP0698082 A4 EP 0698082A4 EP 94915927 A EP94915927 A EP 94915927A EP 94915927 A EP94915927 A EP 94915927A EP 0698082 A4 EP0698082 A4 EP 0698082A4
Authority
EP
European Patent Office
Prior art keywords
hiv
detection
lysis buffer
direct lysis
plasma viremia
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP94915927A
Other languages
English (en)
French (fr)
Other versions
EP0698082A1 (de
Inventor
Denis R Henrard
Jack Phillips
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Publication of EP0698082A1 publication Critical patent/EP0698082A1/de
Publication of EP0698082A4 publication Critical patent/EP0698082A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP94915927A 1993-05-06 1994-04-28 Ein puffer zur direkten lyse und die feststellung der hiv-1 plasma virämie Withdrawn EP0698082A4 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US5862393A 1993-05-06 1993-05-06
US58623 1993-05-06
PCT/US1994/004676 WO1994026867A1 (en) 1993-05-06 1994-04-28 Direct lysis buffer and the detection of hiv-1 plasma viremia

Publications (2)

Publication Number Publication Date
EP0698082A1 EP0698082A1 (de) 1996-02-28
EP0698082A4 true EP0698082A4 (de) 1997-12-17

Family

ID=22017952

Family Applications (1)

Application Number Title Priority Date Filing Date
EP94915927A Withdrawn EP0698082A4 (de) 1993-05-06 1994-04-28 Ein puffer zur direkten lyse und die feststellung der hiv-1 plasma virämie

Country Status (5)

Country Link
EP (1) EP0698082A4 (de)
JP (1) JPH08510004A (de)
AU (1) AU6776994A (de)
CA (1) CA2161337A1 (de)
WO (1) WO1994026867A1 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2184270C (en) * 1994-03-10 1999-08-17 Daniel Louis Kacian Method for suppressing inhibition of enzyme-mediated reactions by ionic detergents
FR2763957B1 (fr) * 1997-05-29 2001-09-14 Bio Merieux Procede pour isoler un materiel intracellulaire et procede de traitement d'un materiel nucleique
US5958677A (en) * 1997-07-28 1999-09-28 The New York Blood Center, Inc. Method for purifying viral nucleic acids
DE19752961C1 (de) * 1997-11-28 1999-07-15 Fraunhofer Ges Forschung Verfahren und Vorrichtung zum Aufschluß biologischer Zellen zur Extraktion und Analyse der Zellinhalte
AU4312201A (en) * 1999-12-10 2001-06-18 Genespan Corporation Isolation and purification of nucleic acids
WO2003064605A2 (en) 2002-01-28 2003-08-07 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
US20050277121A1 (en) 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
DE102005015005A1 (de) 2005-04-01 2006-10-05 Qiagen Gmbh Verfahren zur Behandlung einer Biomoleküle enthaltenden Probe
US7964350B1 (en) 2007-05-18 2011-06-21 Applied Biosystems, Llc Sample preparation for in situ nucleic acid analysis
US8211637B2 (en) 2008-12-19 2012-07-03 Life Technologies Corporation Proteinase K inhibitors, methods and compositions therefor
JP5916610B2 (ja) * 2009-09-03 2016-05-11 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ 直接的な化学的溶解のための方法および組成物
CN109402240B (zh) * 2019-01-08 2020-08-25 圣湘生物科技股份有限公司 核酸释放剂、核酸pcr扩增方法和pcr扩增试剂盒
WO2021221109A1 (ja) * 2020-04-30 2021-11-04 タカラバイオ株式会社 Ｒｎａウイルスの検出方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0338591A2 (de) * 1988-04-21 1989-10-25 Microprobe Corporation Verfahren zum Extrahieren vom Nukleinsäuren
EP0428197A2 (de) * 1989-10-18 1991-05-22 Johnson & Johnson Clinical Diagnostics, Inc. Verfahren zur Nukleinsäureextraktion und PCR-Amplifizierung ohne Gebrauch von proteolytischem Enzym
EP0488243A1 (de) * 1990-11-30 1992-06-03 Sanwa Kagaku Kenkyusho Co., Ltd. Verfahren zur Extraktion des viralen Genoms von einer von einem lebenden Körper abgeleiteten Probe, die mit dem Virus infiziert ist sowie Nachweis des Genoms

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5182377A (en) * 1988-09-09 1993-01-26 Hoffmann-La Roche Inc. Probes for detection of human papillomavirus
US5213796A (en) * 1991-05-06 1993-05-25 Dana Farber Cancer Institute Assay for polyomavirus in humans and uses thereof
US5175094A (en) * 1991-08-01 1992-12-29 Becton Dickinson And Company Increased expression of HBcAg

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0338591A2 (de) * 1988-04-21 1989-10-25 Microprobe Corporation Verfahren zum Extrahieren vom Nukleinsäuren
EP0428197A2 (de) * 1989-10-18 1991-05-22 Johnson & Johnson Clinical Diagnostics, Inc. Verfahren zur Nukleinsäureextraktion und PCR-Amplifizierung ohne Gebrauch von proteolytischem Enzym
EP0488243A1 (de) * 1990-11-30 1992-06-03 Sanwa Kagaku Kenkyusho Co., Ltd. Verfahren zur Extraktion des viralen Genoms von einer von einem lebenden Körper abgeleiteten Probe, die mit dem Virus infiziert ist sowie Nachweis des Genoms

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ALBERT J ET AL: "Simple, sensitive, and specific detection of human immunodeficiency virus type 1 in clinical specimens by polymerase chain reaction with nested primers.", JOURNAL OF CLINICAL MICROBIOLOGY, (1990 JUL) 28 (7) 1560-4., XP002044041 *
See also references of WO9426867A1 *

Also Published As

Publication number Publication date
AU6776994A (en) 1994-12-12
CA2161337A1 (en) 1994-11-24
EP0698082A1 (de) 1996-02-28
WO1994026867A1 (en) 1994-11-24
JPH08510004A (ja) 1996-10-22

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Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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Effective date: 19951030

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Designated state(s): BE CH DE ES FR GB IT LI

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Inventor name: PHILLIPS, JACK

Inventor name: HENRARD, DENIS R.

A4 Supplementary search report drawn up and despatched

Effective date: 19971103

AK Designated contracting states

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Ipc: C12Q 1/68

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18D Application deemed to be withdrawn

Effective date: 20000324