DK0778348T3 - Fremgangsmåde til fremstilling af et protein ved anvendelse af en svamp transformeret ved multikopi-integration af en ekspr - Google Patents

Fremgangsmåde til fremstilling af et protein ved anvendelse af en svamp transformeret ved multikopi-integration af en ekspr

Info

Publication number
DK0778348T3
DK0778348T3 DK97200631T DK97200631T DK0778348T3 DK 0778348 T3 DK0778348 T3 DK 0778348T3 DK 97200631 T DK97200631 T DK 97200631T DK 97200631 T DK97200631 T DK 97200631T DK 0778348 T3 DK0778348 T3 DK 0778348T3
Authority
DK
Denmark
Prior art keywords
protein
preparing
multicopy integration
integration
yeast
Prior art date
Application number
DK97200631T
Other languages
Danish (da)
English (en)
Inventor
Marco Luigi Frederic Giuseppin
Cornelis Theodorus Verrips
Maria Teresa Santos Lopes
Roelf Johannes Planta
Johannes Maria A Verbakel
Original Assignee
Unilever Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB898915659A external-priority patent/GB8915659D0/en
Application filed by Unilever Nv filed Critical Unilever Nv
Application granted granted Critical
Publication of DK0778348T3 publication Critical patent/DK0778348T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DK97200631T 1989-07-07 1990-07-09 Fremgangsmåde til fremstilling af et protein ved anvendelse af en svamp transformeret ved multikopi-integration af en ekspr DK0778348T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB898915659A GB8915659D0 (en) 1989-07-07 1989-07-07 Enzymes,their production and use
EP90201007 1990-04-20

Publications (1)

Publication Number Publication Date
DK0778348T3 true DK0778348T3 (da) 2000-12-04

Family

ID=26125849

Family Applications (2)

Application Number Title Priority Date Filing Date
DK97200631T DK0778348T3 (da) 1989-07-07 1990-07-09 Fremgangsmåde til fremstilling af et protein ved anvendelse af en svamp transformeret ved multikopi-integration af en ekspr
DK90911878.8T DK0481008T3 (da) 1989-07-07 1990-07-09 Fremgangsmåde til fremstilling af et protein ved anvendelse af en svamp transformeret ved multikopiintegration af en ekspressionsvektor.

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK90911878.8T DK0481008T3 (da) 1989-07-07 1990-07-09 Fremgangsmåde til fremstilling af et protein ved anvendelse af en svamp transformeret ved multikopiintegration af en ekspressionsvektor.

Country Status (10)

Country Link
US (1) US6090574A (ja)
EP (2) EP0778348B1 (ja)
JP (1) JP3273609B2 (ja)
AT (2) ATE160176T1 (ja)
CA (1) CA2063592C (ja)
DE (2) DE69033633T2 (ja)
DK (2) DK0778348T3 (ja)
ES (2) ES2109238T3 (ja)
IE (1) IE81016B1 (ja)
WO (1) WO1991000920A2 (ja)

Families Citing this family (99)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991014781A1 (en) * 1990-03-19 1991-10-03 Henkel Research Corporation METHOD FOR INCREASING THE OMEGA-HYDROXYLASE ACTIVITY IN $i(CANDIDA TROPICALIS)
JP3133774B2 (ja) * 1990-10-04 2001-02-13 日本食品化工株式会社 新規な宿主−ベクター系
DE69121159T2 (de) * 1990-10-04 1997-03-13 Japan Maize Prod Wirt-Vektor-System
IT1251435B (it) * 1991-09-04 1995-05-09 Sclavo Spa Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono.
AU5811194A (en) * 1992-12-11 1994-07-04 Quest International B.V. The use of the (kluyveromyces marxianus) inulinase gene promoter for protein production
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
AU725390B2 (en) * 1996-09-24 2000-10-12 Andreas Bachmair DNA-constructs comprising intergenic ribosomal DNA and methods to produce proteins using these DNA-constructs
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
EP0870835A1 (en) * 1997-04-07 1998-10-14 Unilever N.V. Agrobacterium mediated transformation of moulds, in particular those belonging to the genus Aspergillus
JP4307563B2 (ja) 1997-04-07 2009-08-05 ユニリーバー・ナームローゼ・ベンノートシャープ 糸状菌、特にアスペルギルス属に属する糸状菌のアグロバクテリウム媒介性形質転換
ATE398181T1 (de) * 1997-04-11 2008-07-15 Dsm Ip Assets Bv Hefezellen welche zumindest zwei kopien von einem erwünschten gens enthalt und integrierte in dem chromosomal genom auf mehr als einem nicht- ribosomaler rns kodierende bereich, ins besonders mit kluyveromyces
DK0979294T3 (en) * 1997-04-11 2015-08-31 Dsm Ip Assets Bv Gene conversion AS A TOOL FOR CONSTRUCTION OF RECOMBINANT INDUSTRIAL filamentous fungi
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
DE69732378D1 (de) * 1997-05-27 2005-03-03 Hanil Synthetic Fiber Co Ltd Verfahren zur herstellung von rekombinanten eiweissen durch verwendung von hocheffizienten expressionsvektoren aus saccharomyces cerevisiae
US6228583B1 (en) 1997-08-04 2001-05-08 Massachusetts Institute Of Technology Assays for compounds which extend life span
BR9814404A (pt) * 1997-12-22 2000-10-10 Dsm Nv " clonagem de expressão em fungos filamentosos "
CN1284998A (zh) 1997-12-22 2001-02-21 尤尼利弗公司 多拷贝基因在霉菌中的定点整合方法
AU3669499A (en) * 1998-04-28 1999-11-16 Axys Pharmaceuticals, Inc. Novel serine protease capable of selective cleavage of insulin-like growth factor binding protein
DE69922978T2 (de) 1998-10-06 2005-12-08 Emalfarb, Mark Aaron, Jupiter Transformationsystem in filamentösen fungiziden chrysosporium-wirtszellen
US7220542B2 (en) 2000-07-17 2007-05-22 Van Den Brink Johannes Maarten Expression cloning in filamentous fungi
WO2001020007A1 (en) * 1999-09-13 2001-03-22 Concordia University A multifunctional system for the efficient manipulation of protein expression in filamentous fungi and method using same
DE19963690A1 (de) * 1999-11-23 2001-06-13 Rhein Biotech Proz & Prod Gmbh Vektoren und Verfahren zur Herstellung rekombinanter Proteine in Pilzen
WO2002092757A2 (en) * 2000-11-15 2002-11-21 Kemin Industries, Inc. Enzyme over-producing transgenic microorganisms
WO2002048382A2 (en) 2000-12-13 2002-06-20 Unilever N.V. Method for the production of a heterologous protein by a fungus
AU2002226307A1 (en) * 2001-01-25 2002-08-06 Evolva Biotech A/S Artificial chromosomes comprising concatemers of expressible nucleotide sequences
ATE501251T1 (de) 2001-01-25 2011-03-15 Evolva Ltd Zellbibliothek
CA2444960C (en) 2001-05-18 2011-08-09 Danisco A/S Method of improving dough and bread quality
ITMI20011728A1 (it) * 2001-08-06 2003-02-06 Consorzio Per Le Ricerche E Lo Vettore per l'integrazione sito-specifica di sequenze di dna eterologhe in lieviti metilotrofi
WO2004072225A2 (en) * 2003-02-12 2004-08-26 Ramot At Tel Aviv University Ltd. Transgenic fungi expressing bcl-2 and methods of using bcl-2 or portions thereof for improving biomass production, survival, longevity, stress resistance and pathogenicity of fungi
ES2539006T3 (es) 2004-07-16 2015-06-25 Dupont Nutrition Biosciences Aps Enzima lipolítica, usos de la misma en la industria alimentaria
WO2008073914A2 (en) 2006-12-10 2008-06-19 Dyadic International Inc. Expression and high-throughput screening of complex expressed dna libraries in filamentous fungi
CA2736661A1 (en) 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes
WO2009065777A1 (en) 2007-11-20 2009-05-28 Dsm Ip Assets B.V. Dicarboxylic acid production in a filamentous fungus
EP2220232B1 (en) 2007-11-20 2014-09-03 DSM IP Assets B.V. Succinic acid production in a eukaryotic cell
ES2389989T3 (es) 2007-11-20 2012-11-05 Dsm Ip Assets B.V. Producción de ácido dicarboxílico en levadura recombinante
EP2116136A1 (en) 2008-05-08 2009-11-11 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO Novel phytases
NZ591972A (en) * 2008-10-03 2013-04-26 Agrisoma Biosciences Inc Production of modified fatty acids in plants
EP2408907B8 (en) 2009-03-17 2018-04-18 Dutch DNA Biotech B.V. Use of cellulose and ligno-cellulose active proteins
EP2419518A1 (en) 2009-04-15 2012-02-22 DSM IP Assets B.V. Dicarboxylic acid production process
UA108853C2 (uk) 2009-07-10 2015-06-25 Спосіб ферментації галактози
DE102009035671A1 (de) * 2009-07-30 2011-02-03 Dechema Gesellschaft Für Chemische Technik Und Biotechnologie E.V. Verfahren zur Expression von homologen Proteinen und/oder Peptiden in Pilzen der Klasse Dothideomycetes
PL2561064T3 (pl) 2010-04-21 2018-03-30 Dsm Ip Assets B.V. Komórka odpowiednia do fermentacji kompozycji mieszanych cukrów
EP2388331A1 (en) 2010-05-21 2011-11-23 Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO Constitutive promoter
MY160995A (en) 2010-10-13 2017-03-28 Dsm Ip Assets Bv Polypeptides with permease activity
MX2013012269A (es) 2011-04-22 2013-11-22 Dsm Ip Assets Bv Celula de levadura capaz de convertir azucares que incluyen arabinosa y xilosa.
AR087423A1 (es) 2011-08-04 2014-03-19 Dsm Ip Assets Bv Celula capaz de fermentar azucares pentosas
EP2554668A1 (en) 2011-08-04 2013-02-06 DSM IP Assets B.V. A pentose sugar fermenting cell
CA2862980A1 (en) 2012-01-23 2013-08-01 Dsm Ip Assets B.V. Diterpene production
US20130273619A1 (en) 2012-04-16 2013-10-17 Basf Se Process for the Preparation of (3E, 7E)-Homofarnesol
AR093025A1 (es) 2012-10-16 2015-05-13 Dsm Ip Assets Bv Celulas con conversion mejorada de pentosas
EP2772545A1 (en) 2013-03-01 2014-09-03 Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO Starch active proteins
US9885066B2 (en) 2013-05-02 2018-02-06 Dutch Dna Biotech B.V. Organic acid pathway
MX367033B (es) 2013-05-31 2019-08-02 Dsm Ip Assets Bv Microorganismos para la produccion de diterpeno.
CA2914900A1 (en) 2013-07-15 2015-01-22 Dsm Ip Assets B.V. Recombinant microorganism and process for the production of steviol glycosides
WO2015014959A1 (en) 2013-07-31 2015-02-05 Dsm Ip Assets B.V. Recovery of steviol glycosides
US10443077B2 (en) 2015-02-16 2019-10-15 Dsm Ip Assets B.V. Fermentation process for producing itaconic acid under nitrogen free conditions
EP3277829B1 (en) 2015-04-03 2020-07-08 DSM IP Assets B.V. Steviol glycosides
WO2016172282A1 (en) 2015-04-21 2016-10-27 Dsm Ip Assets B.V. Microbial production of terpenoids
CA2983776A1 (en) 2015-05-22 2016-12-01 Paul Klaassen Acetate consuming yeast cell
US11066688B2 (en) 2015-07-10 2021-07-20 Dsm Ip Assets B.V. Method for preparing a steviol glycoside composition
US20180230505A1 (en) 2015-08-13 2018-08-16 Dsm Ip Assets B.V. Steviol glycoside transport
WO2017050652A1 (en) 2015-09-25 2017-03-30 Dsm Ip Assets B.V. Asparaginase
EP3359655B1 (en) 2015-10-06 2019-11-20 DSM IP Assets B.V. Eukaryotic cell with increased production of fermentation product
WO2017167847A1 (en) 2016-03-31 2017-10-05 Dsm Ip Assets B.V. Production of milk with low lactose content
WO2017167849A1 (en) 2016-03-31 2017-10-05 Dsm Ip Assets B.V. Enzyme composition and preparation of a dairy product with improved properties
EP3435771A1 (en) 2016-03-31 2019-02-06 DSM IP Assets B.V. Enzyme composition and preparation of a dairy product with improved properties
CN109312296B (zh) 2016-06-14 2023-05-05 帝斯曼知识产权资产管理有限公司 重组酵母细胞
DK3485004T3 (da) 2016-07-13 2021-12-13 Dsm Ip Assets Bv Malatdehyrogenaser
WO2018029274A1 (en) 2016-08-09 2018-02-15 Dsm Ip Assets B.V. Crystallization of steviol glycosides
WO2018029272A1 (en) 2016-08-09 2018-02-15 Dsm Ip Assets B.V. Crystallization of steviol glycosides
US11066681B2 (en) 2016-08-26 2021-07-20 Lesaffre Et Compagnie Production of itaconic acid
WO2018073107A1 (en) 2016-10-19 2018-04-26 Dsm Ip Assets B.V. Eukaryotic cell comprising xylose isomerase
WO2018114912A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
EP3559222A1 (en) 2016-12-21 2019-10-30 DSM IP Assets B.V. Lipolytic enzyme variants
WO2018114940A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114938A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114995A1 (en) 2016-12-22 2018-06-28 Dsm Ip Assets B.V. Fermentation process for producing steviol glycosides
CN111032854A (zh) 2017-06-13 2020-04-17 帝斯曼知识产权资产管理有限公司 重组酵母细胞
WO2019006312A1 (en) * 2017-06-30 2019-01-03 Myriant Corporation MICROORGANISM HAVING A NUMBER OF STABILIZED COPIES OF A FUNCTIONAL DNA SEQUENCE AND ASSOCIATED METHODS
US11414683B2 (en) 2017-09-26 2022-08-16 Dsm Ip Assets B.V. Acetic acid consuming strain
EP3533878A1 (en) 2018-02-28 2019-09-04 Dutch DNA Biotech B.V. Process for producing citramalic acid employing aspergillus
EP3809852A1 (en) 2018-06-19 2021-04-28 DSM IP Assets B.V. Lipolytic enzyme variants
CN111254128A (zh) * 2018-11-30 2020-06-09 康码（上海）生物科技有限公司 蛋白激酶a催化亚基tpk1基因改造方法及其应用
CN111254127B (zh) * 2018-11-30 2022-06-10 康码（上海）生物科技有限公司 蛋白激酶a催化亚基tpk2基因改造方法及其应用
WO2022084482A1 (en) 2020-10-22 2022-04-28 Dsm Ip Assets B.V. Microorganisms for diterpene production
FR3122436A1 (fr) * 2021-04-30 2022-11-04 IFP Energies Nouvelles Insertion multicopies d’un gène d’intérêt dans le génome d’un champignon
EP4359426A2 (en) 2021-06-24 2024-05-01 Fonterra Co-Operative Group Limited Recombinant beta-lactoglobulin
WO2023285280A1 (en) 2021-07-12 2023-01-19 Dsm Ip Assets B.V. Recombinant yeast cell
WO2023285294A1 (en) 2021-07-12 2023-01-19 Dsm Ip Assets B.V. Recombinant yeast cell
EP4370651A1 (en) 2021-07-12 2024-05-22 Danisco Us Inc Recombinant yeast cell
EP4370690A1 (en) 2021-07-12 2024-05-22 Danisco Us Inc Recombinant yeast cell
EP4370691A1 (en) 2021-07-12 2024-05-22 Danisco US Inc. Recombinant yeast cell
CN117916381A (zh) 2021-07-12 2024-04-19 丹尼斯科美国公司 重组酵母细胞
CN113564206B (zh) * 2021-07-29 2023-09-26 山东大学 一种目的基因多拷贝整合到酿酒酵母染色体rDNA的方法
WO2023079050A1 (en) 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Recombinant yeast cell
WO2023079049A1 (en) 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Variant polypeptide and recombinant yeast cell
WO2023079048A1 (en) 2021-11-04 2023-05-11 Dsm Ip Assets B.V. Process for the production of ethanol and recombinant yeast cell
AU2022388727A1 (en) 2021-11-09 2024-05-16 Amgen Inc. Production of therapeutic proteins
WO2023222614A1 (en) 2022-05-16 2023-11-23 Dsm Ip Assets B.V. Lipolytic enzyme variants

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU600885B2 (en) * 1984-05-25 1990-08-30 Zymogenetics Inc. Stable DNA constructs
GB8415186D0 (en) * 1984-06-14 1984-07-18 Ciba Geigy Ag Polypeptides
CA1341130C (en) * 1984-07-27 2000-10-31 Adrianus Marinus Ledeboer Use of oxidoreductases in bleaching and/or detergent compositions and their preparation by microorganisms engineered by recombinant dna technology
JPS61134325A (ja) * 1984-12-04 1986-06-21 Teijin Ltd ハイブリツド抗体遺伝子の発現方法
JPH0614865B2 (ja) * 1985-12-13 1994-03-02 麒麟麦酒株式会社 α―アセト乳酸脱炭酸酵素をコードするＤＮＡ鎖およびこのＤＮＡ鎖により形質転換された酵母
KR910002604B1 (ko) 1986-05-28 1991-04-27 미쓰비시덴기 가부시기가이샤 영상 재생 장치
CA1339101C (en) * 1986-06-03 1997-07-29 Nicolaas Overbeeke Production of guar alpha-galactosidase and immunologically related alpha-galactosidases by host organisms transformed with recombinant dna methods
GB8629536D0 (en) 1986-12-10 1987-01-21 Unilever Plc Enzymatic detergent composition
PT88556B (pt) * 1987-09-28 1992-11-30 Smithkline Biolog Processo de integracao genomica estavel e de expressao de uma sequencia de cadificacao estranha, em leveduras
DK571587D0 (da) 1987-11-02 1987-11-02 Novo Industri As Enzymatisk detergentsammensaetning
EP0318775B1 (en) * 1987-12-03 1994-02-02 Chisso Corporation A lipase gene
US5001230A (en) * 1988-02-18 1991-03-19 Schering Corporation T cell activation markers
JP3079276B2 (ja) * 1988-02-28 2000-08-21 天野製薬株式会社 組換え体ｄｎａ、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法
JP2639677B2 (ja) * 1988-03-14 1997-08-13 旭化成工業株式会社 リパーゼの遺伝情報を有するｄｎａ

Also Published As

Publication number Publication date
EP0481008B1 (en) 1997-11-12
ATE196505T1 (de) 2000-10-15
EP0481008A1 (en) 1992-04-22
DE69031710D1 (de) 1997-12-18
WO1991000920A3 (en) 1991-03-07
ATE160176T1 (de) 1997-11-15
DK0481008T3 (da) 1998-05-04
US6090574A (en) 2000-07-18
ES2109238T3 (es) 1998-01-16
JP3273609B2 (ja) 2002-04-08
EP0778348A1 (en) 1997-06-11
JPH05501949A (ja) 1993-04-15
DE69033633T2 (de) 2001-05-03
CA2063592A1 (en) 1991-01-08
WO1991000920A2 (en) 1991-01-24
CA2063592C (en) 2003-01-21
ES2150188T3 (es) 2000-11-16
IE81016B1 (en) 1999-09-22
DE69031710T2 (de) 1998-03-05
EP0778348B1 (en) 2000-09-20
IE902496A1 (en) 1991-02-13
DE69033633D1 (de) 2000-10-26

Similar Documents

Publication Publication Date Title
DK0778348T3 (da) Fremgangsmåde til fremstilling af et protein ved anvendelse af en svamp transformeret ved multikopi-integration af en ekspr
ATE86663T1 (de) Glucoamylasegen.
KR950701348A (ko) 재조합 사람 락토페린 생산방법
BR8200191A (pt) Celula viva renina preprorrenina prorrenina processo para obter expressao de preporrenina prorrenina e renina gene de prorrenina preprorrenina e renina e coli material dna recombinador saccharomyces cerevisiae polipetidio processo para exprimir um gene
ATE125865T1 (de) Rekombinante humicola-lipase und verfahren zur herstellung von rekombinanten humicola-lipasen.
EP0945515A3 (en) Production of proteins using homologous recombination
DE3584149D1 (de) Verfahren zur herstellung von alkohol.
IE880558L (en) Stable gene amplification in prokaryotic chromosomal dna
PL326087A1 (en) Improved integration of exogenic dna introduced into eucariotic cells
IE802742L (en) Alpha-galactosidase
CA2037677A1 (en) Process for production of exogenous gene or its product in plant cells
EP0128743A3 (en) Expression of foreign genes in yeast
DE69409354T3 (de) Dextranase, Herstellungsverfahren und dafür kodierende DNS
DE3570694D1 (en) Recombinant saccharomyces
FI871584A (fi) Uppbyggande av nya -galaktosidas producerande jaeststammar och foerfaranden foer industriellt utnyttjande av dylika jaeststammar.
ES8602933A1 (es) Un metodo para clonar por tecnicas de dna recombinante penicilinacilasas e incrementar su produccion por fermentacion
IT1228924B (it) Clonaggio del gene dell'isoamilasi.