DK0515506T3 - Fremgangsmåde til påvisning af carcinogene humane papillomavirus - Google Patents

Fremgangsmåde til påvisning af carcinogene humane papillomavirus

Info

Publication number
DK0515506T3
DK0515506T3 DK91904707T DK91904707T DK0515506T3 DK 0515506 T3 DK0515506 T3 DK 0515506T3 DK 91904707 T DK91904707 T DK 91904707T DK 91904707 T DK91904707 T DK 91904707T DK 0515506 T3 DK0515506 T3 DK 0515506T3
Authority
DK
Denmark
Prior art keywords
pcr
enzymatic
pcr reagents
amplification
mixtures
Prior art date
Application number
DK91904707T
Other languages
Danish (da)
English (en)
Inventor
Will Bloch
Jonathan Raymond
Alan R Read
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of DK0515506T3 publication Critical patent/DK0515506T3/da

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C59/00—Surface shaping of articles, e.g. embossing; Apparatus therefor
    • B29C59/10—Surface shaping of articles, e.g. embossing; Apparatus therefor by electric discharge treatment
    • B29C59/103—Surface shaping of articles, e.g. embossing; Apparatus therefor by electric discharge treatment of profiled articles, e.g. hollow or tubular articles
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
    • C08J7/04—Coating
    • C08J7/0427—Coating with only one layer of a composition containing a polymer binder
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
    • C08J7/04—Coating
    • C08J7/046—Forming abrasion-resistant coatings; Forming surface-hardening coatings
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2471/00—Characterised by the use of polyethers obtained by reactions forming an ether link in the main chain; Derivatives of such polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
    • G01N2035/00247—Microvalves
    • G01N2035/00267—Meltable plugs
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00277—Special precautions to avoid contamination (e.g. enclosures, glove- boxes, sealed sample carriers, disposal of contaminated material)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DK91904707T 1990-02-16 1991-02-15 Fremgangsmåde til påvisning af carcinogene humane papillomavirus DK0515506T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48150190A 1990-02-16 1990-02-16
PCT/US1991/001039 WO1991012342A1 (fr) 1990-02-16 1991-02-15 Ameliorations apportees a la specificite et a l'applicabilite de la reaction en chaine de polymerases

Publications (1)

Publication Number Publication Date
DK0515506T3 true DK0515506T3 (da) 2000-05-08

Family

ID=23912174

Family Applications (1)

Application Number Title Priority Date Filing Date
DK91904707T DK0515506T3 (da) 1990-02-16 1991-02-15 Fremgangsmåde til påvisning af carcinogene humane papillomavirus

Country Status (10)

Country Link
US (1) US5411876A (fr)
EP (1) EP0515506B1 (fr)
JP (2) JP3087907B2 (fr)
AT (1) ATE188509T1 (fr)
AU (1) AU653712B2 (fr)
CA (1) CA2075050C (fr)
DE (1) DE69131891T2 (fr)
DK (1) DK0515506T3 (fr)
ES (1) ES2141088T3 (fr)
WO (1) WO1991012342A1 (fr)

Families Citing this family (172)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5674679A (en) * 1991-09-27 1997-10-07 Amersham Life Science, Inc. DNA cycle sequencing
WO1993015222A1 (fr) * 1992-01-29 1993-08-05 Institut National De La Sante Et De La Recherche Medicale (Inserm) Procede pour eviter les risques de contamination, notamment applicable aux techniques d'amplification de l'adn ou de l'arn
US5413924A (en) * 1992-02-13 1995-05-09 Kosak; Kenneth M. Preparation of wax beads containing a reagent for release by heating
EP0572057A1 (fr) * 1992-05-11 1993-12-01 Johnson & Johnson Clinical Diagnostics, Inc. Composition et kit de réactifs de PCR et procédés pour amplification et détection avec amplification non-spécifique d'acides nucléiques réduite
GB9211979D0 (en) * 1992-06-05 1992-07-15 Buchard Ole Uses of nucleic acid analogues
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
AU6029694A (en) * 1993-01-19 1994-08-15 Pharmacia P-L Biochemicals, Inc. Storage and delivery of purified protein reagents with carrier wax
US5599660A (en) * 1993-01-19 1997-02-04 Pharmacia Biotech Inc. Method and preparation for sequential delivery of wax-embedded, inactivated biological and chemical reagents
WO1995011315A1 (fr) * 1993-10-22 1995-04-27 Invitrogen Corporation Incorporation dans de la cire de composants reactifs en vue de leur liberation a des temperatures specifiees
EP1245286B1 (fr) * 1993-10-22 2009-11-25 Abbott Laboratories Tube de réaction et méthode d'utilisation de celui-ci minimisant les contaminations
AU2871095A (en) * 1994-06-24 1996-01-19 University Of Houston, The Encapsulated pcr reagents
ZA956776B (en) * 1994-08-25 1996-03-20 Akzo Nobel Nv A process for making the product of a nucleic acid amplification reaction incapable of being a target for further amplification a diagnostic assay employing said process a kit and a container suitable for carrying out said process for assay
US5622822A (en) 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5576197A (en) * 1995-04-07 1996-11-19 Molecular Bio-Products Polymerase chain reaction container and methods of using the same
US5837442A (en) 1995-11-29 1998-11-17 Roche Molecular Systems, Inc. Oligonucleotide primers for amplifying HCV nucleic acid
US5721123A (en) * 1996-01-05 1998-02-24 Microfab Technology, Inc. Methods and apparatus for direct heating of biological material
US5763184A (en) 1996-01-30 1998-06-09 Roche Molecular Systems, Inc. Nucleotide sequence variation in the ABO glycosyltransferase gene
AU4065397A (en) * 1996-08-14 1998-03-06 Life Technologies, Inc. Stable compositions for nucleic acid amplification and sequencing
JP3182527B2 (ja) * 1996-09-03 2001-07-03 株式会社ヤトロン 化学発光の測定方法及びキット
US6110710A (en) * 1996-10-15 2000-08-29 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Sequence modification of oligonucleotide primers to manipulate non-templated nucleotide addition
CA2792122C (fr) * 1997-07-07 2015-09-08 Medical Research Council Procede de selection in vitro
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
US8337753B2 (en) 1998-05-01 2012-12-25 Gen-Probe Incorporated Temperature-controlled incubator having a receptacle mixing mechanism
CA2754182A1 (fr) 1998-05-01 1999-11-11 Gen-Probe Incorporated Analyseur automatise pour diagnostics et procede correspondant
US6183998B1 (en) 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
US20020055118A1 (en) * 1998-06-24 2002-05-09 Yong-Bin Eym Method of preparing objects containing DNA
ES2153745B1 (es) * 1998-07-31 2001-11-16 Ivia Dispositivo compartimentado y metodo para la captura y doble amplificacion enzimatica de secuencias diana de acidos nucleicos en un solo dispositivo compartimentado cerrado.
US6413780B1 (en) * 1998-10-14 2002-07-02 Abbott Laboratories Structure and method for performing a determination of an item of interest in a sample
US6200757B1 (en) 1999-01-19 2001-03-13 Dade Behring Inc. Method for controlling the extension of an oligonucleotide
BR0009164A (pt) * 1999-03-19 2001-12-26 Genencor Int Placa de teste de múltiplos furos vazados paraseparação de alto desempenho
ES2228520T3 (es) 1999-05-04 2005-04-16 Ortho-Clinical Diagnostics, Inc. Capatura rapida y eficaz de adn de una muestra sin usar reactivo de lisis celular.
US6643787B1 (en) 1999-10-19 2003-11-04 Rambus Inc. Bus system optimization
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
US7262006B1 (en) 2000-05-01 2007-08-28 Ortho-Clinical Diagnostics, Inc. Rapid and efficient capture of DNA from sample without using cell lysing reagent
ES2300346T3 (es) * 2000-08-03 2008-06-16 F. Hoffmann-La Roche Ag Compuestos de enlace de acidos nucleicos que contienen analogos de pirazolo-3,4-d pirimidina de purina 2,6-diamina y sus usos.
IL155450A0 (en) * 2000-10-20 2003-11-23 Expression Diagnostics Inc Leukocyte expression profiling
AU2002212691A1 (en) * 2000-10-27 2002-05-06 Supercritical Co., Ltd. Method of gene amplification
FR2817961B1 (fr) * 2000-12-08 2003-08-01 Diagast Barriere de separation temporaire, recipient la comprenant et procede de mise en oeuvre d'un test dans ce recipient
US6428962B1 (en) 2001-02-12 2002-08-06 Dna Analysis, Inc. Nucleic acid collection barrier method and apparatus
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en) 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
WO2002101042A1 (fr) * 2001-06-12 2002-12-19 Takara Bio Inc. Procede de stabilisation d'un reactif pour amplifier ou detecter l'acide nucleique et procede de memorisation
US7226732B2 (en) * 2001-07-16 2007-06-05 Cepheid Methods, apparatus, and computer programs for verifying the integrity of a probe
US6403341B1 (en) 2001-08-02 2002-06-11 Wayne M. Barnes Magnesium precipitate hot start method for PCR
US20030027196A1 (en) * 2001-08-02 2003-02-06 Barnes Wayne M. Magnesium precipitate methods for magnesium dependent enzymes
WO2004011666A2 (fr) * 2002-07-26 2004-02-05 Applera Corporation Reactions biochimiques a demarrage a chaud induit par mg
US8277753B2 (en) * 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
EP1403379A1 (fr) * 2002-09-24 2004-03-31 QIAGEN GmbH Coamplification améliorée d'acides nucléiques
EP1608952B1 (fr) 2002-12-20 2016-08-10 Life Technologies Corporation Appareil et procede de dosage utilisant des reseaux microfluidiques
CA2727850C (fr) * 2003-01-29 2013-04-30 454 Corporation Procede d'amplification et de sequencage d'acides nucleiques
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
WO2004094986A2 (fr) * 2003-04-16 2004-11-04 Handylab, Inc. Systeme et procede de detection electrochimique de composes biologiques
US7892745B2 (en) * 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
WO2004101733A1 (fr) * 2003-05-01 2004-11-25 Southeast University Dispositif d'amplification pcr pour reaction a etapes multiples et tube d'amplification pcr sans lavage pour detection de genes
EP1953246A3 (fr) 2003-05-01 2008-11-19 Veridex, LLC Analyse de nýud lymphoïde intraopératif
WO2005070027A2 (fr) * 2004-01-23 2005-08-04 Biomerieux, Inc. Nouveau mélange de nucléotides permettant d'améliorer l'amplification des acides nucléiques
EP1559478B1 (fr) * 2004-01-23 2010-06-16 Qiagen GmbH Barrière contre contamination
JP2007529015A (ja) 2004-03-12 2007-10-18 バイオトローブ， インコーポレイテッド ナノリットルのアレイローディング
DE102004021822B3 (de) * 2004-04-30 2005-11-17 Siemens Ag Verfahren und Anordnung zur DNA-Amplifikation mittels PCR unter Einsatz von Trockenreagenzien
US20090298052A1 (en) * 2004-06-04 2009-12-03 David Atkins Diagnosing or Predicting the Course of Breast Cancer
US20060019366A1 (en) * 2004-06-09 2006-01-26 Wanli Bi Reversibly modified thermostable enzyme compositions and methods of making and using the same
US20070154889A1 (en) * 2004-06-25 2007-07-05 Veridex, Llc Methods and reagents for the detection of melanoma
US7700281B2 (en) * 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
GB0414815D0 (en) * 2004-07-02 2004-08-04 Secr Defence Method for stabilising reagents which are useful for nucleic acid amplification
GB2416352B (en) * 2004-07-21 2009-01-28 Bioline Ltd A method for performing the hot start of enzymatic reactions
US20060029801A1 (en) * 2004-08-03 2006-02-09 O'brien Robert Method for making a coated powder for reducing evaporative water loss
US7645575B2 (en) * 2004-09-08 2010-01-12 Xdx, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
EP2418018B1 (fr) * 2004-12-23 2013-05-22 Abbott Point of Care Inc. Procédés de séparation d'acides nucléiques
CN101155917A (zh) * 2005-03-30 2008-04-02 株式会社岛津制作所 基因多态性诊断用装置
EP1885889A4 (fr) * 2005-05-11 2010-01-20 Expression Diagnostics Inc Procedes de surveillance de l'etat fonctionnel de transplants a l'aide de panels de genes
WO2007002588A2 (fr) * 2005-06-23 2007-01-04 Applera Corporation Pointe de pipette a cyclage thermique
EP2405024A1 (fr) 2005-07-15 2012-01-11 Life Technologies Corporation Transcription inverse à amorçage chaud par une conception d'amorce
US7745122B2 (en) * 2005-07-15 2010-06-29 Applied Biosystems, Llc Analyzing messenger RNA and micro RNA in the same reaction mixture
US7727473B2 (en) 2005-10-19 2010-06-01 Progentech Limited Cassette for sample preparation
US7754148B2 (en) 2006-12-27 2010-07-13 Progentech Limited Instrument for cassette for sample preparation
GB0600228D0 (en) 2006-01-06 2006-02-15 Fermentas Uab Inactivation method
ATE510027T1 (de) * 2006-02-27 2011-06-15 Hoffmann La Roche Pcr-heissstart durch magnesium-sequestrierung
EP3167900B1 (fr) 2006-03-29 2018-11-21 Merial Limited Vaccin contre des streptocoques
CA2648580A1 (fr) * 2006-04-07 2008-05-02 Xdx, Inc. Expression d'acide nucleique en reponse aux steroides et prevision de l'activite d'une maladie
ES2376202T3 (es) * 2006-07-31 2012-03-09 Wanli Bi Amplificación de �?cidos nucleicos usando un oligonucleótido modificado reversiblemente.
US9045522B2 (en) 2006-07-31 2015-06-02 Wanli Bi Nucleic acid amplification using a reversibly modified oligonucleotide
US7993832B2 (en) * 2006-08-14 2011-08-09 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
WO2008140484A2 (fr) * 2006-11-09 2008-11-20 Xdx, Inc. Procédés pour diagnostiquer et surveiller l'état d'un lupus érythémateux systémique
WO2008065996A1 (fr) * 2006-11-28 2008-06-05 Shimadzu Corporation Plaque de réaction
JP4307478B2 (ja) * 2006-12-05 2009-08-05 キヤノン株式会社 生化学反応用カートリッジ
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
WO2008122843A2 (fr) * 2007-04-10 2008-10-16 Pontes Luis Andre Utilisation de barrières liquides pour effectuer une pcr à démarrage à chaud
WO2008134470A2 (fr) * 2007-04-25 2008-11-06 3M Innovative Properties Company Procédés d'amplification d'acides nucléiques
JPWO2008146686A1 (ja) * 2007-05-25 2010-08-19 ベックマン・コールター・インコーポレーテッド 酵素含有カプセル及び核酸の増幅キット
WO2008146649A1 (fr) * 2007-05-25 2008-12-04 Olympus Corporation Gel contenant une enzyme, et coffret d'amplification d'acide nucléique
JP2010537637A (ja) 2007-08-27 2010-12-09 アプライド バイオシステムズ インコーポレイテッド Ｐｃｒのための方法および組成物
WO2009054473A1 (fr) * 2007-10-26 2009-04-30 Toppan Printing Co., Ltd. Puce de réaction, procédé de réaction, unité de régulation de température pour un appareil de traitement de gène et appareil de traitement de gène
CA2658520C (fr) 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Amplification d'acide nucleique en presence d'oligonucleotides randomises modifies
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
AU2009242546B2 (en) 2008-04-30 2015-01-22 Integrated Dna Technologies, Inc. RNase-H-based assays utilizing modified RNA monomers
EP2990487A1 (fr) 2008-05-08 2016-03-02 Asuragen, INC. Compositions et procédés relatifs à la modulation de miarn de néovascularisation ou angiogenèse
US7910720B2 (en) 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
IT1397110B1 (it) * 2008-12-29 2012-12-28 St Microelectronics Rousset Microreattore autosigillante e metodo per eseguire una reazione
JP5608998B2 (ja) * 2009-03-31 2014-10-22 東洋紡株式会社 保存安定性に優れた核酸増幅検出試薬キット
JP5608997B2 (ja) * 2009-03-31 2014-10-22 東洋紡株式会社 保存安定性に優れた核酸増幅検出試薬キット
WO2010117461A2 (fr) * 2009-04-08 2010-10-14 Applied Biosystems, Llc Système et procédé de préparation et d'utilisation d'une émulsion en vrac
EP2432895A2 (fr) 2009-05-22 2012-03-28 Asuragen, INC. Biomarqueurs miarn d'une maladie prostatique
IL206810A (en) 2009-07-08 2015-07-30 Janssen Diagnostics Llc Methods for identifying melanoma and kits for using these methods
JP5229160B2 (ja) * 2009-08-26 2013-07-03 株式会社島津製作所 反応容器
US8735074B2 (en) 2009-08-28 2014-05-27 Asuragen, Inc. MiRNA biomarkers of lung disease
WO2011060014A1 (fr) * 2009-11-13 2011-05-19 Integrated Dna Technologies, Inc. Essais de détection de petits arn
US9238832B2 (en) 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
EP4043546A1 (fr) 2010-02-23 2022-08-17 Luminex Corporation Appareil et méthodes pour la préparation, la réaction et la détection intégrées d'échantillons
SI23321A (sl) 2010-03-03 2011-09-30 Inštitut za fizikalno biologijo d.o.o. Izboljĺ ano zbiranje fluorescentne svetlobe iz vzorca med pomnoĺ˝evanjem v rt-pcr
DK201000356A (en) * 2010-04-22 2011-10-23 Statens Seruminstitut New nucleotide sequence amplification Method
JP5454338B2 (ja) * 2010-04-28 2014-03-26 株式会社島津製作所 液滴操作によるリアルタイム核酸増幅法
CA2798123C (fr) 2010-05-05 2020-06-23 The Governing Council Of The University Of Toronto Procede de traitement d'echantillons seches utilisant un dispositif microfluidique numerique
KR20130087492A (ko) 2010-06-22 2013-08-06 유니바사루 바이오 리사치 가부시키가이샤 핵산 증폭 반응 중의 반응 용액의 증발을 방지하기 위한 조성물
CN103589627B (zh) 2010-07-23 2015-11-18 贝克曼考尔特公司 用于在pcr反应容器内进行实时pcr的热循环器模块和系统
US20120045799A1 (en) * 2010-08-17 2012-02-23 Rheonix, Inc. Thermocycler seal composition, method, and application
WO2012068400A2 (fr) 2010-11-17 2012-05-24 Asuragen, Inc. Micro-arn utilisés comme biomarqueurs pour faire la distinction entre des néoplasmes thyroïdiens bénins et malins
AU2012236896A1 (en) 2011-03-25 2013-05-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
US9469871B2 (en) 2011-04-14 2016-10-18 Corporos Inc. Methods and apparatus for point-of-care nucleic acid amplification and detection
US8911941B2 (en) 2011-04-14 2014-12-16 Kenneth J. Michlitsch Methods and apparatus for point-of-care nucleic acid amplification and detection
EP2705130B1 (fr) 2011-05-04 2016-07-06 Luminex Corporation Appareil et procédé pour la préparation, la réaction et la détection intégrées d'échantillons
US20120288866A1 (en) * 2011-05-11 2012-11-15 Genturadx, Inc. Systems and methods for producing an evaporation barrier in a reaction chamber
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
US8475739B2 (en) * 2011-09-25 2013-07-02 Theranos, Inc. Systems and methods for fluid handling
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
US20140170735A1 (en) 2011-09-25 2014-06-19 Elizabeth A. Holmes Systems and methods for multi-analysis
MX2014002990A (es) 2011-09-19 2014-05-21 Genentech Inc Tratamientos combinados que comprenden antagonistas de c-met y antagonistas de b-raf.
JP6050820B2 (ja) 2011-09-23 2016-12-21 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改良された対立遺伝子特異的ｐｃｒのためのｇ−クランプの使用
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
US10012664B2 (en) 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
GB2561425B (en) 2012-03-16 2019-01-16 Cambridge Entpr Ltd Apparatus for obtaining liquid from a solid phase
US9354159B2 (en) 2012-05-02 2016-05-31 Nanoscopia (Cayman), Inc. Opto-fluidic system with coated fluid channels
US9085761B1 (en) 2012-06-14 2015-07-21 Affymetrix, Inc. Methods and compositions for amplification of nucleic acids
DE102012222351A1 (de) 2012-12-05 2014-06-05 Gna Biosolutions Gmbh Reaktionsgefäß mit magnetischem Verschluss
CA2905949A1 (fr) 2013-03-15 2014-09-25 Baylor Research Institute Marqueurs d'une neoplasie colorectale associee a la colite ulcereuse (uc)
WO2014145612A1 (fr) 2013-03-15 2014-09-18 Ajay Goel Biomarqueurs de miarn à base de tissu et de sang pour le diagnostic, le pronostic et le potentiel prédictif de métastases dans le cancer colorectal
US11545241B1 (en) 2013-09-07 2023-01-03 Labrador Diagnostics Llc Systems and methods for analyte testing and data management
JP6706208B2 (ja) 2014-01-05 2020-06-03 バイオマーナ ホールディングス リミテッド Ｍｉｒｎａ比率を使用する肺がん決定
WO2015120382A1 (fr) 2014-02-07 2015-08-13 The Johns Hopkins University Prédiction de réponse à un traitement utilisant des médicaments épigénétiques
CN106604774B (zh) 2014-06-16 2019-10-25 生命技术公司 试剂混合器及流体控制装置
EP3636342B1 (fr) 2014-06-17 2021-04-28 Life Technologies Corporation Procédé de préparation d'une cartouche réactive
WO2016037051A1 (fr) 2014-09-04 2016-03-10 Theranos, Inc. Pathogène et test de résistance antimicrobienne
WO2016094330A2 (fr) 2014-12-08 2016-06-16 20/20 Genesystems, Inc Procédés et systèmes d'apprentissage par machine pour prédire la probabilité ou le risque d'avoir le cancer
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
CN104845967B (zh) 2015-04-15 2020-12-11 苏州新海生物科技股份有限公司 寡聚核苷酸片段及使用其的选择性扩增目标核酸序列变异体的方法及应用
CN107849603B (zh) 2015-04-24 2022-01-28 阿提拉生物系统公司 利用有限核苷酸组成的引物扩增
WO2016177774A1 (fr) 2015-05-04 2016-11-10 Academisch Medisch Centrum Procédé de quantification des mi-arn à l'aide de la normalisation
US10464067B2 (en) 2015-06-05 2019-11-05 Miroculus Inc. Air-matrix digital microfluidics apparatuses and methods for limiting evaporation and surface fouling
CN208562324U (zh) 2015-06-05 2019-03-01 米罗库鲁斯公司 空气基质数字微流控(dmf)装置
WO2017040171A1 (fr) * 2015-08-31 2017-03-09 Siemens Healthcare Diagnostics Inc. Procédé et dispositif destinés à la prévention d'éclaboussures d'un échantillon de fluide non recouvert pendant le transport sur un équipement de laboratoire de diagnostic
KR20180103945A (ko) * 2016-02-05 2018-09-19 젠-프로브 인코포레이티드 건조된 증폭 조성물
WO2017223026A1 (fr) * 2016-06-20 2017-12-28 Miroculus Inc. Détection d'arn à l'aide de procédés d'amplification facilitée par boucle et induite par ligature et microfluidique numérique
CN109715781A (zh) 2016-08-22 2019-05-03 米罗库鲁斯公司 用于数字微流控设备中的并行液滴控制的反馈系统
US10427162B2 (en) 2016-12-21 2019-10-01 Quandx Inc. Systems and methods for molecular diagnostics
CN110383061A (zh) 2016-12-28 2019-10-25 米罗库鲁斯公司 数字微流控设备和方法
US11623219B2 (en) 2017-04-04 2023-04-11 Miroculus Inc. Digital microfluidics apparatuses and methods for manipulating and processing encapsulated droplets
US20180320218A1 (en) * 2017-05-08 2018-11-08 Smobio Technology, Inc. Kit for polymerase chain reaction
US11413617B2 (en) 2017-07-24 2022-08-16 Miroculus Inc. Digital microfluidics systems and methods with integrated plasma collection device
JP7341124B2 (ja) 2017-09-01 2023-09-08 ミロキュラス インコーポレイテッド デジタルマイクロ流体デバイスおよびその使用方法
CA3096855A1 (fr) 2018-05-23 2019-11-28 Miroculus Inc. Controle de l'evaporation dans la microfluidique numerique
EP3719144A1 (fr) 2019-04-05 2020-10-07 Fundación para la Investigación Biomédica del Hospital Universitario de la Paz (FIBHULP) Mir-151a-3p en tant que contrôle endogène universel pour la normalisation d'un chargement d'exosomes
US11738345B2 (en) 2019-04-08 2023-08-29 Miroculus Inc. Multi-cartridge digital microfluidics apparatuses and methods of use
WO2021013592A1 (fr) 2019-07-19 2021-01-28 Fundación Para La Investigación Biomédica Del Hospital Universitario La Paz (Fibhulp) Procédé de détermination de la réponse au traitement d'un patient atteint d'un carcinome pulmonaire non à petites cellules (nsclc)
US11524298B2 (en) 2019-07-25 2022-12-13 Miroculus Inc. Digital microfluidics devices and methods of use thereof
CN113373202A (zh) * 2020-03-10 2021-09-10 苏州先达基因科技有限公司 防污染单管核酸恒温扩增检测系统
CN114657236B (zh) * 2020-12-23 2024-06-18 广州达安基因股份有限公司 一种分隔核酸扩增体系的方法和分隔剂
CN113680406B (zh) * 2021-08-26 2022-04-15 清华大学 一种微流控芯片多指标检测方法
US11772093B2 (en) 2022-01-12 2023-10-03 Miroculus Inc. Methods of mechanical microfluidic manipulation

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE100617C (fr) *
US3919085A (en) * 1974-02-27 1975-11-11 Becton Dickinson Co Plasma separator assembly
US4032492A (en) * 1976-02-25 1977-06-28 H. B. Fuller Company Hot melt container closure sealant
AU520409B2 (en) * 1977-05-25 1982-01-28 Commonwealth Scientific And Industrial Research Organisation Controlled release composition
US4522786A (en) * 1983-08-10 1985-06-11 E. I. Du Pont De Nemours And Company Multilayered test device for detecting analytes in liquid test samples
DE3416094A1 (de) * 1984-04-30 1985-10-31 Hilti Ag, Schaan Zerstoerbare patrone fuer mehrkomponenten-massen
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
CA1338457C (fr) * 1986-08-22 1996-07-16 Henry A. Erlich Enzyme thermostable purifiee
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
ATE121794T1 (de) * 1987-02-26 1995-05-15 Univ Sydney Verfahren zum nachweis des karzinogenen menschlichen papillomavirus.
CA1338505C (fr) * 1989-02-03 1996-08-06 John Bruce Findlay Cuvette de retenue pour pcr et methode d'utilisation

Also Published As

Publication number Publication date
JP3532826B2 (ja) 2004-05-31
JP3087907B2 (ja) 2000-09-18
DE69131891T2 (de) 2000-06-15
ATE188509T1 (de) 2000-01-15
ES2141088T3 (es) 2000-03-16
CA2075050C (fr) 1998-10-06
EP0515506B1 (fr) 2000-01-05
JP2000316561A (ja) 2000-11-21
DE69131891D1 (de) 2000-02-10
JPH05506143A (ja) 1993-09-16
CA2075050A1 (fr) 1991-08-17
WO1991012342A1 (fr) 1991-08-22
AU7307391A (en) 1991-09-03
EP0515506A1 (fr) 1992-12-02
US5411876A (en) 1995-05-02
AU653712B2 (en) 1994-10-13

Similar Documents

Publication Publication Date Title
DK0515506T3 (da) Fremgangsmåde til påvisning af carcinogene humane papillomavirus
Malorny et al. Standardization of diagnostic PCR for the detection of foodborne pathogens
Koo et al. CRISPR/dCas9-mediated biosensor for detection of tick-borne diseases
Morris et al. Reconstruction of bacteriophage T4 DNA replication apparatus from purified components: rolling circle replication following de novo chain initiation on a single-stranded circular DNA template.
Lowery et al. Characterization of the nucleoside triphosphate phosphohydrolase (ATPase) activity of RNA synthesis termination factor p. II. Influence of synthetic RNA homopolymers and random copolymers on the reaction.
DK10787A (da) Fremgangsmaade til detektering eller undersoegelse af tilstedevaerelse eller fravaer af en nucleinsyresekvens, som i det vaesentlige er bevaret, blandt isolater fra virus samt kit til anvendelse ved udoebelse af fremgangsmaaden
EP0875584A3 (fr) Analyse des acides nucléiques
Miyaoka et al. Detection and quantification of HDR and NHEJ induced by genome editing at endogenous gene loci using droplet digital PCR
DK0726324T3 (da) Anvendelse af exonuklease og/eller glycosylase som supplementer til antipolymeraseantistof til forøgelse af specification i polymerasekædereaktion
WO1999060159A3 (fr) Generation de produits d'amplification de l'acide nucleique par utilisation de concentrations inegales d'amorces
EP0379369A3 (fr) Amplification d'acides nucléiques utilisant une amorce
WO2006076017A2 (fr) Procedes et kits d'identification de nucleotides cibles dans des populations melangees
CA2074069A1 (fr) Amorces et procede de depistage des genotypes du papillomavirus humain par pcr
WO2001092569A3 (fr) Formulation pour amplification en chaine par polymerase et recipient contenant celle-ci
KR900016473A (ko) 진단 키트, 프라이머 조성물 및 이것을 이용해 핵산을 복제하거나 검사하는 방법
KR910006497A (ko) 프라이머 3' 말단에서의 프라이머-표적 오합(mismatch)을 극복한 진단 및 증폭방법
WO1996007100A3 (fr) Technique de marquage a la luciferase
WO2019136034A8 (fr) Test basé sur une amplification isothermique à médiation par boucle (lamp) pour la détection de microbes
ATE417129T1 (de) Nukleinsäureamplifikation mit nichtstandardbasen
DeWit et al. Simple method for production of internal control DNA for Mycobacterium tuberculosis polymerase chain reaction assays
Theunissen et al. DNA profiling of sperm cells by using micromanipulation and whole genome amplification
Tews et al. Application of the C4'-alkylated deoxyribose primer system (CAPS) in allele-specific real-time PCR for increased selectivity in discrimination of single nucleotide sequence variants
Saunders Quantitative real-time PCR
Yang et al. ATPase activity measurement of DNA replicative helicase from Bacillus stearothermophilus by malachite green method
GB9918237D0 (en) Detection system