DE69838106T2 - Expressionsklonierung in filamentösen pilzen - Google Patents

Expressionsklonierung in filamentösen pilzen Download PDF

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Publication number
DE69838106T2
DE69838106T2 DE69838106T DE69838106T DE69838106T2 DE 69838106 T2 DE69838106 T2 DE 69838106T2 DE 69838106 T DE69838106 T DE 69838106T DE 69838106 T DE69838106 T DE 69838106T DE 69838106 T2 DE69838106 T2 DE 69838106T2
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Germany
Prior art keywords
vector
expression
dna
cloning
cdna
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Expired - Lifetime
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DE69838106T
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German (de)
English (en)
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DE69838106D1 (de
Inventor
Johannes Maarten Van Den Brink
Gerard Cornelis Selten
Johannes Petrus T. W. Van Den Hombergh
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DSM IP Assets BV
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DSM IP Assets BV
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8229117&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69838106(T2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
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Publication of DE69838106T2 publication Critical patent/DE69838106T2/de
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1086Preparation or screening of expression libraries, e.g. reporter assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Mushroom Cultivation (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Peptides Or Proteins (AREA)
DE69838106T 1997-12-22 1998-12-22 Expressionsklonierung in filamentösen pilzen Expired - Lifetime DE69838106T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP97204079 1997-12-22
EP97204079 1997-12-22
PCT/EP1998/008577 WO1999032617A2 (en) 1997-12-22 1998-12-22 Expression cloning in filamentous fungi

Publications (2)

Publication Number Publication Date
DE69838106D1 DE69838106D1 (de) 2007-08-30
DE69838106T2 true DE69838106T2 (de) 2008-04-03

Family

ID=8229117

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69838106T Expired - Lifetime DE69838106T2 (de) 1997-12-22 1998-12-22 Expressionsklonierung in filamentösen pilzen

Country Status (13)

Country Link
US (1) US20020155536A1 (https=)
EP (2) EP1854890A1 (https=)
JP (3) JP4410413B2 (https=)
CN (1) CN1198924C (https=)
AT (1) ATE367439T1 (https=)
AU (1) AU736111C (https=)
BR (1) BR9814404A (https=)
CA (1) CA2313445C (https=)
DE (1) DE69838106T2 (https=)
DK (1) DK1042461T3 (https=)
ES (1) ES2287989T3 (https=)
PL (1) PL341760A1 (https=)
WO (1) WO1999032617A2 (https=)

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AU5354401A (en) * 2000-04-13 2001-10-30 Emalfarb, Mark Aaron High-throughput screening of expressed DNA libraries in filamentous fungi
US7514110B1 (en) 2000-09-21 2009-04-07 Basf Aktiengesellschaft Talaromyces xylanases
ATE475324T1 (de) 2000-12-07 2010-08-15 Dsm Ip Assets Bv Mit carboxy-terminal prolin peptide angereicherte proteinhydrolysate
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WO2004078919A2 (en) * 2003-02-27 2004-09-16 Midwest Research Institute Superactive cellulase formulation using cellobiohydrolase-1 from penicillium funiculosum
US20050059024A1 (en) 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
EP1776461A2 (en) * 2004-04-16 2007-04-25 DSMIP Assets B.V. Fungal promoters for expressing a gene in a fungal cell
US9045748B2 (en) 2004-05-27 2015-06-02 Novozymes, Inc. Methods for transforming and expression screening of filamentous fungal cells with a DNA library
EA014853B1 (ru) * 2004-10-15 2011-02-28 ДСМ АйПи АССЕТС Б.В. Амидаза из aspergillus niger и применение амидазы для получения пищевого продукта с пониженным содержанием акриламида
US7888489B2 (en) 2005-01-24 2011-02-15 Dsm Ip Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
JP2008532535A (ja) * 2005-03-18 2008-08-21 ディーエスエム アイピー アセッツ ビー.ブイ. 宿主細胞における改善された発現クローニング法
EP1954812B1 (en) 2005-11-29 2012-11-21 DSM IP Assets B.V. Dna binding site of a transcriptional activator useful in gene expression
WO2007063133A2 (en) 2005-12-01 2007-06-07 Dsm Ip Assets B.V. Genes useful for the industrial production of citric acid
WO2008000632A1 (en) 2006-06-29 2008-01-03 Dsm Ip Assets B.V. A method for achieving improved polypeptide expression
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EP2505651A3 (en) 2006-12-10 2013-01-09 Dyadic International, Inc. Isolated fungus with reduced protease activity
EA018840B1 (ru) 2007-02-15 2013-11-29 ДСМ АйПи АССЕТС Б.В. Рекомбинантная клетка-хозяин для получения соединения, представляющего интерес
CA2676686C (en) 2007-02-20 2015-12-15 Dsm Ip Assets B.V. Novel sialidase
US8551751B2 (en) 2007-09-07 2013-10-08 Dyadic International, Inc. BX11 enzymes having xylosidase activity
CN102741421B (zh) 2009-03-10 2015-03-25 帝斯曼知识产权资产管理有限公司 用于提高多肽产率的方法
DK2406372T3 (da) 2009-03-10 2017-11-27 Dsm Ip Assets Bv Prægastrisk esterase og derivater deraf
EP2421986A1 (en) 2009-04-22 2012-02-29 DSM IP Assets B.V. Process for the production of a recombinant polypeptide of interest
EP3293264A1 (en) 2009-07-22 2018-03-14 DSM IP Assets B.V. Improved host cell for the production of a compound of interest
IN2012DN03186A (https=) 2009-11-04 2015-09-25 Dsm Ip Assets Bv
EP2531590A2 (en) 2010-02-04 2012-12-12 DSM IP Assets B.V. Process for preparing filamentous fungal strains having a sexual cycle and a process for preparing sexually crossed filamentous fungal strains
EA201201125A1 (ru) 2010-02-11 2013-03-29 ДСМ АйПи АССЕТС Б.В. Клетка-хозяин, способная продуцировать ферменты, пригодные для деградации лигноцеллюлозного материала
AU2011273351A1 (en) 2010-07-01 2013-01-17 Dsm Ip Assets B.V. A method for the production of a compound of interest
WO2012007446A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Use of a mutarotase in the production of dried powders
WO2012007445A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Mutarotase in crystallization
US20150197760A1 (en) 2012-06-19 2015-07-16 Dsm Ip Assets B.V. Promoters for expressing a gene in a cell
JP6468561B2 (ja) 2012-07-19 2019-02-13 ディーエスエム アイピー アセッツ ビー.ブイ.Dsm Ip Assets B.V. AgsE欠損株
PL3686280T3 (pl) 2013-02-04 2022-09-19 Dsm Ip Assets B.V. Polipeptyd degradujący węglowodany i jego zastosowania
WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
WO2014202624A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202620A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202622A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202621A1 (en) 2013-06-20 2014-12-24 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
DK3077517T3 (en) 2013-12-02 2019-02-11 Dsm Ip Assets Bv ISSTRUCTURING PROTEIN
CN105940103B (zh) * 2014-01-31 2021-06-15 株式会社Api 哌可酸4位羟化酶和利用该酶的4-羟基氨基酸的制造法
WO2015177153A1 (en) 2014-05-19 2015-11-26 Dsm Ip Assets B.V. Proline-specific endoprotease
US10450554B2 (en) 2014-05-19 2019-10-22 Dsm Ip Assets B.V. Proline specific endoprotease
WO2015177152A1 (en) 2014-05-19 2015-11-26 Dsm Ip Assets B.V. Proline-specific endoprotease
WO2016110453A1 (en) 2015-01-06 2016-07-14 Dsm Ip Assets B.V. A crispr-cas system for a filamentous fungal host cell
AR104205A1 (es) 2015-04-09 2017-07-05 Dsm Ip Assets Bv Fosfolipasa c
WO2016193292A1 (en) 2015-06-02 2016-12-08 Dsm Ip Assets B.V. Use of ice structuring protein afp19 expressed in filamentous fungal strains for preparing food
EP4361240A3 (en) 2015-11-17 2024-07-31 DSM IP Assets B.V. Preparation of a stable beer
WO2018019948A1 (en) 2016-07-29 2018-02-01 Dsm Ip Assets B.V. Polypeptides having cellulolytic enhancing activity and uses thereof
ES2784318T3 (es) * 2016-09-20 2020-09-24 Clariant Int Ltd Método para una expresión selectiva independiente de la fuente de carbono de secuencias que codifican una proteína en una célula de hongo filamentoso
US20200199599A1 (en) 2016-09-23 2020-06-25 Dsm Ip Assets B.V. A guide-rna expression system for a host cell
WO2018114938A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114941A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114912A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114940A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
US20200063166A1 (en) 2017-03-13 2020-02-27 Dsm Ip Assets B.V. Zinc binuclear cluster transcriptional regulator-deficient strain
CN110462044A (zh) 2017-04-06 2019-11-15 帝斯曼知识产权资产管理有限公司 自引导整合构建体(sgic)
WO2019215078A1 (en) 2018-05-07 2019-11-14 Dsm Ip Assets B.V. Process for enzymatic oil degumming
BR112020025589A2 (pt) 2018-06-19 2021-03-23 Dsm Ip Assets B.V. variantes de enzima lipolítica
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Also Published As

Publication number Publication date
CN1198924C (zh) 2005-04-27
JP2002509698A (ja) 2002-04-02
EP1042461B1 (en) 2007-07-18
CA2313445A1 (en) 1999-07-01
WO1999032617A2 (en) 1999-07-01
JP2005261438A (ja) 2005-09-29
US20020155536A1 (en) 2002-10-24
EP1042461A2 (en) 2000-10-11
AU2515199A (en) 1999-07-12
ATE367439T1 (de) 2007-08-15
DK1042461T3 (da) 2007-09-24
JP4410413B2 (ja) 2010-02-03
EP1854890A1 (en) 2007-11-14
CA2313445C (en) 2012-03-20
ES2287989T3 (es) 2007-12-16
BR9814404A (pt) 2000-10-10
JP2010046080A (ja) 2010-03-04
DE69838106D1 (de) 2007-08-30
CN1283227A (zh) 2001-02-07
WO1999032617A3 (en) 1999-09-10
AU736111B2 (en) 2001-07-26
AU736111C (en) 2004-02-19
PL341760A1 (en) 2001-05-07

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