AU736111C - Expression cloning in filamentous fungi - Google Patents
Expression cloning in filamentous fungiInfo
- Publication number
- AU736111C AU736111C AU25151/99A AU2515199A AU736111C AU 736111 C AU736111 C AU 736111C AU 25151/99 A AU25151/99 A AU 25151/99A AU 2515199 A AU2515199 A AU 2515199A AU 736111 C AU736111 C AU 736111C
- Authority
- AU
- Australia
- Prior art keywords
- protein
- expression
- interest
- vector
- properties
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Mycology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Mushroom Cultivation (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP97204079 | 1997-12-22 | ||
| EP97204079 | 1997-12-22 | ||
| PCT/EP1998/008577 WO1999032617A2 (en) | 1997-12-22 | 1998-12-22 | Expression cloning in filamentous fungi |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| AU2515199A AU2515199A (en) | 1999-07-12 |
| AU736111B2 AU736111B2 (en) | 2001-07-26 |
| AU736111C true AU736111C (en) | 2004-02-19 |
Family
ID=8229117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU25151/99A Ceased AU736111C (en) | 1997-12-22 | 1998-12-22 | Expression cloning in filamentous fungi |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US20020155536A1 (https=) |
| EP (2) | EP1854890A1 (https=) |
| JP (3) | JP4410413B2 (https=) |
| CN (1) | CN1198924C (https=) |
| AT (1) | ATE367439T1 (https=) |
| AU (1) | AU736111C (https=) |
| BR (1) | BR9814404A (https=) |
| CA (1) | CA2313445C (https=) |
| DE (1) | DE69838106T2 (https=) |
| DK (1) | DK1042461T3 (https=) |
| ES (1) | ES2287989T3 (https=) |
| PL (1) | PL341760A1 (https=) |
| WO (1) | WO1999032617A2 (https=) |
Families Citing this family (66)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7883872B2 (en) | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
| CN100378218C (zh) | 1999-10-01 | 2008-04-02 | 诺沃奇梅兹有限公司 | 喷雾干燥的酶产品 |
| KR20020026456A (ko) | 2000-04-13 | 2002-04-10 | 에말파브 마크 아론 | 사상균에서 발현된 dna 라이브러리의 고산출량 스크리닝 |
| AU5354401A (en) * | 2000-04-13 | 2001-10-30 | Emalfarb, Mark Aaron | High-throughput screening of expressed DNA libraries in filamentous fungi |
| US7514110B1 (en) | 2000-09-21 | 2009-04-07 | Basf Aktiengesellschaft | Talaromyces xylanases |
| ATE475324T1 (de) | 2000-12-07 | 2010-08-15 | Dsm Ip Assets Bv | Mit carboxy-terminal prolin peptide angereicherte proteinhydrolysate |
| AU2002358179A1 (en) | 2001-12-21 | 2003-07-09 | Dsm Ip Assets B.V. | New rennets |
| TWI328612B (en) | 2002-07-25 | 2010-08-11 | Dsm Ip Assets Bv | Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12 |
| WO2004078919A2 (en) * | 2003-02-27 | 2004-09-16 | Midwest Research Institute | Superactive cellulase formulation using cellobiohydrolase-1 from penicillium funiculosum |
| US20050059024A1 (en) | 2003-07-25 | 2005-03-17 | Ambion, Inc. | Methods and compositions for isolating small RNA molecules |
| EP1776461A2 (en) * | 2004-04-16 | 2007-04-25 | DSMIP Assets B.V. | Fungal promoters for expressing a gene in a fungal cell |
| US9045748B2 (en) | 2004-05-27 | 2015-06-02 | Novozymes, Inc. | Methods for transforming and expression screening of filamentous fungal cells with a DNA library |
| EA014853B1 (ru) * | 2004-10-15 | 2011-02-28 | ДСМ АйПи АССЕТС Б.В. | Амидаза из aspergillus niger и применение амидазы для получения пищевого продукта с пониженным содержанием акриламида |
| US7888489B2 (en) | 2005-01-24 | 2011-02-15 | Dsm Ip Assets B.V. | Method for producing a compound of interest in a filamentous fungal cell |
| JP2008532535A (ja) * | 2005-03-18 | 2008-08-21 | ディーエスエム アイピー アセッツ ビー.ブイ. | 宿主細胞における改善された発現クローニング法 |
| EP1954812B1 (en) | 2005-11-29 | 2012-11-21 | DSM IP Assets B.V. | Dna binding site of a transcriptional activator useful in gene expression |
| WO2007063133A2 (en) | 2005-12-01 | 2007-06-07 | Dsm Ip Assets B.V. | Genes useful for the industrial production of citric acid |
| WO2008000632A1 (en) | 2006-06-29 | 2008-01-03 | Dsm Ip Assets B.V. | A method for achieving improved polypeptide expression |
| EP2511372A1 (en) | 2006-11-02 | 2012-10-17 | DSM IP Assets B.V. | Improved production of secreted proteins by filamentous fungi |
| EP2505651A3 (en) | 2006-12-10 | 2013-01-09 | Dyadic International, Inc. | Isolated fungus with reduced protease activity |
| EA018840B1 (ru) | 2007-02-15 | 2013-11-29 | ДСМ АйПи АССЕТС Б.В. | Рекомбинантная клетка-хозяин для получения соединения, представляющего интерес |
| CA2676686C (en) | 2007-02-20 | 2015-12-15 | Dsm Ip Assets B.V. | Novel sialidase |
| US8551751B2 (en) | 2007-09-07 | 2013-10-08 | Dyadic International, Inc. | BX11 enzymes having xylosidase activity |
| CN102741421B (zh) | 2009-03-10 | 2015-03-25 | 帝斯曼知识产权资产管理有限公司 | 用于提高多肽产率的方法 |
| DK2406372T3 (da) | 2009-03-10 | 2017-11-27 | Dsm Ip Assets Bv | Prægastrisk esterase og derivater deraf |
| EP2421986A1 (en) | 2009-04-22 | 2012-02-29 | DSM IP Assets B.V. | Process for the production of a recombinant polypeptide of interest |
| EP3293264A1 (en) | 2009-07-22 | 2018-03-14 | DSM IP Assets B.V. | Improved host cell for the production of a compound of interest |
| IN2012DN03186A (https=) | 2009-11-04 | 2015-09-25 | Dsm Ip Assets Bv | |
| EP2531590A2 (en) | 2010-02-04 | 2012-12-12 | DSM IP Assets B.V. | Process for preparing filamentous fungal strains having a sexual cycle and a process for preparing sexually crossed filamentous fungal strains |
| EA201201125A1 (ru) | 2010-02-11 | 2013-03-29 | ДСМ АйПи АССЕТС Б.В. | Клетка-хозяин, способная продуцировать ферменты, пригодные для деградации лигноцеллюлозного материала |
| AU2011273351A1 (en) | 2010-07-01 | 2013-01-17 | Dsm Ip Assets B.V. | A method for the production of a compound of interest |
| WO2012007446A1 (en) | 2010-07-13 | 2012-01-19 | Dsm Ip Assets B.V. | Use of a mutarotase in the production of dried powders |
| WO2012007445A1 (en) | 2010-07-13 | 2012-01-19 | Dsm Ip Assets B.V. | Mutarotase in crystallization |
| US20150197760A1 (en) | 2012-06-19 | 2015-07-16 | Dsm Ip Assets B.V. | Promoters for expressing a gene in a cell |
| JP6468561B2 (ja) | 2012-07-19 | 2019-02-13 | ディーエスエム アイピー アセッツ ビー.ブイ.Dsm Ip Assets B.V. | AgsE欠損株 |
| PL3686280T3 (pl) | 2013-02-04 | 2022-09-19 | Dsm Ip Assets B.V. | Polipeptyd degradujący węglowodany i jego zastosowania |
| WO2014142647A1 (en) | 2013-03-14 | 2014-09-18 | Wageningen Universiteit | Fungals strains with improved citric acid and itaconic acid production |
| WO2014202624A2 (en) | 2013-06-19 | 2014-12-24 | Dsm Ip Assets B.V. | Rasamsonia gene and use thereof |
| WO2014202620A2 (en) | 2013-06-19 | 2014-12-24 | Dsm Ip Assets B.V. | Rasamsonia gene and use thereof |
| WO2014202622A2 (en) | 2013-06-19 | 2014-12-24 | Dsm Ip Assets B.V. | Rasamsonia gene and use thereof |
| WO2014202621A1 (en) | 2013-06-20 | 2014-12-24 | Dsm Ip Assets B.V. | Carbohydrate degrading polypeptide and uses thereof |
| DK3077517T3 (en) | 2013-12-02 | 2019-02-11 | Dsm Ip Assets Bv | ISSTRUCTURING PROTEIN |
| CN105940103B (zh) * | 2014-01-31 | 2021-06-15 | 株式会社Api | 哌可酸4位羟化酶和利用该酶的4-羟基氨基酸的制造法 |
| WO2015177153A1 (en) | 2014-05-19 | 2015-11-26 | Dsm Ip Assets B.V. | Proline-specific endoprotease |
| US10450554B2 (en) | 2014-05-19 | 2019-10-22 | Dsm Ip Assets B.V. | Proline specific endoprotease |
| WO2015177152A1 (en) | 2014-05-19 | 2015-11-26 | Dsm Ip Assets B.V. | Proline-specific endoprotease |
| WO2016110453A1 (en) | 2015-01-06 | 2016-07-14 | Dsm Ip Assets B.V. | A crispr-cas system for a filamentous fungal host cell |
| AR104205A1 (es) | 2015-04-09 | 2017-07-05 | Dsm Ip Assets Bv | Fosfolipasa c |
| WO2016193292A1 (en) | 2015-06-02 | 2016-12-08 | Dsm Ip Assets B.V. | Use of ice structuring protein afp19 expressed in filamentous fungal strains for preparing food |
| EP4361240A3 (en) | 2015-11-17 | 2024-07-31 | DSM IP Assets B.V. | Preparation of a stable beer |
| WO2018019948A1 (en) | 2016-07-29 | 2018-02-01 | Dsm Ip Assets B.V. | Polypeptides having cellulolytic enhancing activity and uses thereof |
| ES2784318T3 (es) * | 2016-09-20 | 2020-09-24 | Clariant Int Ltd | Método para una expresión selectiva independiente de la fuente de carbono de secuencias que codifican una proteína en una célula de hongo filamentoso |
| US20200199599A1 (en) | 2016-09-23 | 2020-06-25 | Dsm Ip Assets B.V. | A guide-rna expression system for a host cell |
| WO2018114938A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| WO2018114941A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| WO2018114912A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| WO2018114940A1 (en) | 2016-12-21 | 2018-06-28 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| US20200063166A1 (en) | 2017-03-13 | 2020-02-27 | Dsm Ip Assets B.V. | Zinc binuclear cluster transcriptional regulator-deficient strain |
| CN110462044A (zh) | 2017-04-06 | 2019-11-15 | 帝斯曼知识产权资产管理有限公司 | 自引导整合构建体(sgic) |
| WO2019215078A1 (en) | 2018-05-07 | 2019-11-14 | Dsm Ip Assets B.V. | Process for enzymatic oil degumming |
| BR112020025589A2 (pt) | 2018-06-19 | 2021-03-23 | Dsm Ip Assets B.V. | variantes de enzima lipolítica |
| EP4055170A1 (en) | 2019-11-04 | 2022-09-14 | DSM IP Assets B.V. | Low volume transfection |
| JP2021093966A (ja) * | 2019-12-18 | 2021-06-24 | 本田技研工業株式会社 | 糸状菌への遺伝子導入方法及びその利用 |
| AU2022297881A1 (en) | 2021-06-24 | 2024-01-18 | Fonterra Co-Operative Group Limited | Recombinant beta-lactoglobulin |
| US20250304938A1 (en) | 2022-05-16 | 2025-10-02 | Dsm Ip Assets B.V. | Lipolytic enzyme variants |
| WO2025012325A1 (en) | 2023-07-11 | 2025-01-16 | Dsm Ip Assets B.V. | Protein arginine deiminase |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK122686D0 (da) * | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| DE69032616T2 (de) * | 1989-06-16 | 1999-03-11 | Genencor International, Inc., Rochester, N.Y. | Dna-sequenzen, vektoren und fusionspolypeptide zur erhöhung der sekretion gewünschter polypeptide von filamentösen fungi |
| WO1991000920A2 (en) * | 1989-07-07 | 1991-01-24 | Unilever N.V. | Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector |
| JPH0595787A (ja) * | 1991-10-04 | 1993-04-20 | Jozo Shigen Kenkyusho:Kk | ホスホグリセリン酸キナ−ゼ遺伝子プロモ−タ−、 同タ−ミネ−タ−または/及びこれらからなる 遺伝子発現用ユニツト、並びに、ホスホグリセリ ン酸キナ−ゼのゲノム遺伝子及びcDNA遺伝子 |
| MX9206979A (es) * | 1991-12-04 | 1993-07-01 | Novo Nordisk As | Metodo de clonacion de proteinas en levaduras |
| JPH05268964A (ja) * | 1992-03-30 | 1993-10-19 | Meiji Milk Prod Co Ltd | 糸状菌プロモーター |
| DE69432543T2 (de) * | 1993-07-23 | 2003-12-24 | Dsm N.V., Te Heerlen | Selektionmarker-genfreie rekombinante Stämme: Verfahren zur ihrer Herstellung und die Verwendung dieser Stämme |
| JP3638657B2 (ja) * | 1995-03-29 | 2005-04-13 | 株式会社資生堂 | Dna修復酵素 |
| CA2219136A1 (en) * | 1995-04-24 | 1996-10-31 | Chromaxome Corp. | Methods for generating and screening novel metabolic pathways |
| JP3343567B2 (ja) * | 1995-06-29 | 2002-11-11 | 大関株式会社 | 糸状菌のエンハンサーdna塩基配列およびその用途 |
| DK0873398T3 (da) * | 1996-01-10 | 2005-08-15 | Novozymes As | Fremgangsmåde til in vivo produktion af et mutantbibliotek i celler |
-
1998
- 1998-12-22 EP EP07112086A patent/EP1854890A1/en not_active Withdrawn
- 1998-12-22 EP EP98966868A patent/EP1042461B1/en not_active Revoked
- 1998-12-22 WO PCT/EP1998/008577 patent/WO1999032617A2/en not_active Ceased
- 1998-12-22 AT AT98966868T patent/ATE367439T1/de active
- 1998-12-22 DE DE69838106T patent/DE69838106T2/de not_active Expired - Lifetime
- 1998-12-22 DK DK98966868T patent/DK1042461T3/da active
- 1998-12-22 CN CN98812563.3A patent/CN1198924C/zh not_active Expired - Lifetime
- 1998-12-22 PL PL98341760A patent/PL341760A1/xx unknown
- 1998-12-22 CA CA2313445A patent/CA2313445C/en not_active Expired - Fee Related
- 1998-12-22 AU AU25151/99A patent/AU736111C/en not_active Ceased
- 1998-12-22 ES ES98966868T patent/ES2287989T3/es not_active Expired - Lifetime
- 1998-12-22 JP JP2000525536A patent/JP4410413B2/ja not_active Expired - Fee Related
- 1998-12-22 BR BR9814404-9A patent/BR9814404A/pt not_active Application Discontinuation
-
2001
- 2001-11-05 US US09/993,164 patent/US20020155536A1/en active Pending
-
2005
- 2005-05-25 JP JP2005152703A patent/JP2005261438A/ja active Pending
-
2009
- 2009-10-20 JP JP2009241780A patent/JP2010046080A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN1198924C (zh) | 2005-04-27 |
| JP2002509698A (ja) | 2002-04-02 |
| EP1042461B1 (en) | 2007-07-18 |
| CA2313445A1 (en) | 1999-07-01 |
| WO1999032617A2 (en) | 1999-07-01 |
| JP2005261438A (ja) | 2005-09-29 |
| US20020155536A1 (en) | 2002-10-24 |
| EP1042461A2 (en) | 2000-10-11 |
| AU2515199A (en) | 1999-07-12 |
| ATE367439T1 (de) | 2007-08-15 |
| DK1042461T3 (da) | 2007-09-24 |
| JP4410413B2 (ja) | 2010-02-03 |
| EP1854890A1 (en) | 2007-11-14 |
| CA2313445C (en) | 2012-03-20 |
| ES2287989T3 (es) | 2007-12-16 |
| BR9814404A (pt) | 2000-10-10 |
| JP2010046080A (ja) | 2010-03-04 |
| DE69838106D1 (de) | 2007-08-30 |
| CN1283227A (zh) | 2001-02-07 |
| WO1999032617A3 (en) | 1999-09-10 |
| AU736111B2 (en) | 2001-07-26 |
| PL341760A1 (en) | 2001-05-07 |
| DE69838106T2 (de) | 2008-04-03 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| CB | Opposition filed |
Opponent name: NOVOZYMES A/S |
|
| DA2 | Applications for amendment section 104 |
Free format text: THE NATURE OF THE PROPOSED AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 20030304 |
|
| DA3 | Amendments made section 104 |
Free format text: THE NATURE OF THE AMENDMENT IS AS WAS NOTIFIED IN THE OFFICIAL JOURNAL DATED 20030925 |
|
| PC1 | Assignment before grant (sect. 113) |
Owner name: DSM IP ASSETS B.V. Free format text: THE FORMER OWNER WAS: DSM N.V. |
|
| ON | Decision of a delegate or deputy of the commissioner of patents (result of patent office hearing) |
Free format text: CLAIMS LACK FAIR BASIS. BECAUSE THE CLAIMS OMITTED AN ESSENTIAL FEATURE OF THE INVENTION, SOME OF THEM ALSO DEFINED THE PRIOR ART AND WERE RENDERED NOT NOVEL. THE PRIOR ART ALSO DEPRIVED MOST OF THE CLAIMS OF THEIR INVENTIVE STEP. THE OPPONENT OTHERWISE FAILED TO ESTABLISH THAT ANY OF THE CLAIMS LACKED INVENTIVE STEP IN LIGHT OF THE COMMON GENERAL KNOWLEDGE OR WERE NOT FOR A MANNER OF MANUFACTURE. Opponent name: NOVOZYMES A/S Effective date: 20051011 |